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The map label for this gene is trmL [H]

Identifier: 221229609

GI number: 221229609

Start: 518423

End: 518899

Strand: Reverse

Name: trmL [H]

Synonym: MLBr_00419

Alternate gene names: 221229609

Gene position: 518899-518423 (Counterclockwise)

Preceding gene: 221229610

Following gene: 221229607

Centisome position: 15.88

GC content: 61.43

Gene sequence:

>477_bases
ATGATCAGCAAGATGTTCCGATTGCTGTTTTATACCCCGCGCATTCCGCCAAACACGGGCAACGCCATCCGGACCGCGGC
AGTCACCGGCTGCGCACTACATCTGGTCGAGCCGATTGGCTTCGACCTGTCCAACCCCAAACTTCGACGGGCCGGGCTAG
ACTATCACGACCTGGCTTCGGTCACCGTCCACACCTCACTATCTGAAGCATGGAACGCGCTATCGCCGGCGCGGGTCTTT
GCGTTCAGCACCCTCGCGAACACCTTGTTCACCGACGTCAGCTACCAGGCCGGCGACGTGCTGATGTTCGGCCCCGAACC
GACCGGACTAGACGCGGCAACCCTGGCCGACGACCATATCACTCAGCAGGTGCGCATTCCCATGCTGTCGGGCCGGCGTT
CATTGAACCTGTCCAACGCCGCGGCGGTAGCAGTGTACGAAGCGTGGCGACAACACGGGTATGCCGGGTTGGTCTAA

Upstream 100 bases:

>100_bases
GCGGGAAAACGCCTGGCAATTCAACAAGTATGACGGCTCGCGGCCTATGTAACCAGTTTCCGGTACAATGATACGCAAGC
CTCACACAAAACCACGCGTA

Downstream 100 bases:

>100_bases
AGCCTGCCCTCACCTCGCGGAGTGAAGCGCCGGCGGCGACCATGGTACCTCTAGCAGCGACAAAGCAGGAGGAACCAGCA
CTGGTAACACTGACAGCTCA

Product: putative tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MISKMFRLLFYTPRIPPNTGNAIRTAAVTGCALHLVEPIGFDLSNPKLRRAGLDYHDLASVTVHTSLSEAWNALSPARVF
AFSTLANTLFTDVSYQAGDVLMFGPEPTGLDAATLADDHITQQVRIPMLSGRRSLNLSNAAAVAVYEAWRQHGYAGLV

Sequences:

>Translated_158_residues
MISKMFRLLFYTPRIPPNTGNAIRTAAVTGCALHLVEPIGFDLSNPKLRRAGLDYHDLASVTVHTSLSEAWNALSPARVF
AFSTLANTLFTDVSYQAGDVLMFGPEPTGLDAATLADDHITQQVRIPMLSGRRSLNLSNAAAVAVYEAWRQHGYAGLV
>Mature_158_residues
MISKMFRLLFYTPRIPPNTGNAIRTAAVTGCALHLVEPIGFDLSNPKLRRAGLDYHDLASVTVHTSLSEAWNALSPARVF
AFSTLANTLFTDVSYQAGDVLMFGPEPTGLDAATLADDHITQQVRIPMLSGRRSLNLSNAAAVAVYEAWRQHGYAGLV

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=154, Percent_Identity=51.2987012987013, Blast_Score=159, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17145; Mature: 17145

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKMFRLLFYTPRIPPNTGNAIRTAAVTGCALHLVEPIGFDLSNPKLRRAGLDYHDLAS
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHH
VTVHTSLSEAWNALSPARVFAFSTLANTLFTDVSYQAGDVLMFGPEPTGLDAATLADDHI
EEEECCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHH
TQQVRIPMLSGRRSLNLSNAAAVAVYEAWRQHGYAGLV
HHHEECCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MISKMFRLLFYTPRIPPNTGNAIRTAAVTGCALHLVEPIGFDLSNPKLRRAGLDYHDLAS
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHH
VTVHTSLSEAWNALSPARVFAFSTLANTLFTDVSYQAGDVLMFGPEPTGLDAATLADDHI
EEEECCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHH
TQQVRIPMLSGRRSLNLSNAAAVAVYEAWRQHGYAGLV
HHHEECCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]