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The map label for this gene is otsB2

Identifier: 221229607

GI number: 221229607

Start: 511993

End: 513282

Strand: Reverse

Name: otsB2

Synonym: MLBr_00414

Alternate gene names: 221229607

Gene position: 513282-511993 (Counterclockwise)

Preceding gene: 221229609

Following gene: 221229601

Centisome position: 15.71

GC content: 61.32

Gene sequence:

>1290_bases
ATGCCGGTCACCATAGACCCGCGGCGCCACAGCGCAGCACTGTTCGACCTCGACGCCGTAGTGACCGACACCCCACTCGA
CTCCACAGTGACGCTGGTCCGGCAACTTCAGGGGATCGGTGTGGGTACCGCCGTTTTTTCGACCAACCGCAACAGCCAGG
GCGTGCTGACTGCAACCGGCCTCGACCATCTGTTCCCCGTCCACGTTGACGGCCTAGCTAGCGGAAAAGTCACCATCCTA
GTCGCCGCGGCCAACCGCTTAATGGCGCAACCAGGCCGGTGCGTCGTCGTCGCAGTCGACGCGGCCGGCATAACGGCCGC
ACGCTATGGCGGTTTCGCGCTGGTGCTCGGCCTAGTAATAGGGGAAGATCAGACAGGCCACCGCGACACACTGCGTAACT
CCGGCGCCGACACGGTAGTTGCCGACCTAGGTGAGGTCATCGTCCGAACCGGGGACCGCCGGATGTCGGAACTTCCCGAC
GCGCTGCAGACCCTCGGCTTAACTGACGACCTCACCGCTCGGCAGCCGGCGGTGTTCTTCGACTTCGACGGCACACTGTC
AGACATCGTCGATGATCCCGACTCGGCCAGACCCGTTCCCGGCGCGACCGAAGCATTGCAGAAGTTGGCCACACATTGTC
CAGTGGCCATACTGTCTGGTCGCGATCTCGCAGATGTAATCAAGCGCATCGGCGTGCCCGGAATCTGGTATTCCGGCAGT
CATGGTTTTGAGTCAACTGCACCCGACGGCACACATCATCAAAACGACGCAGCGGAAGCGACGATACCCATACTGGAGCA
AGCGGCGACCCAGCTACGCGACCAACTCGGACCAATTCCGGGAGTCATGGTGGAACACAAGCGTTTTGGCGTAGCAGTGC
ACTACCGTAACGTCGCGCGTGACCGCGTCAACGAGGTCGCAGTGGCGGTGCGCACAGCAGGCCAGCGCAACGCACTTCGG
GTGACAACAGGTCGCGAGGTCATCGAGCTGCGTCCCGACATCGACTGGGACAAGGGAAAAACACTCCATTGGGTGATCGA
TCGCCTGCACCATGCCGGCACTCAAGTAGGCTCAGCTTCGCTCATGCCGATCTGTCTCGGCGACGACATAACCGACGAGG
ACGCGTTCGACGCAGTGCGCCACACCGACGTTGGGGGTATTCCGATCGTGGTACGACACACTGAAGACGGTAACCGCGCA
ACAGCCGCATTGTTCACGTTGGACAGTCCTATGCACGTCAGTGAATTCACCGAACGGTTGGCACGCCAGCTTAGTGACAC
TCAACGCTAG

Upstream 100 bases:

>100_bases
ACCAGTTCAGACGAGCCTCCACGCCTTATGCACGAGCGCGTCGCTAACCGGACATGGAGCTCCCTGCTGACCACAGTTTC
CACCGTGAGATGGTTAGTTA

Downstream 100 bases:

>100_bases
GCCGGCAGGTACTCCAACCGCCCCGTCTTTCAGCACCACCCGCATTTCGTCGCCGCCAAAGCCAACGACCTCAGTACGAA
ACACCGTCCGCCCTGGCTCG

Product: putative trehalose-6-phosphate phosphatase

Products: NA

Alternate protein names: TPP; Trehalose-6-phosphate phosphatase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MPVTIDPRRHSAALFDLDAVVTDTPLDSTVTLVRQLQGIGVGTAVFSTNRNSQGVLTATGLDHLFPVHVDGLASGKVTIL
VAAANRLMAQPGRCVVVAVDAAGITAARYGGFALVLGLVIGEDQTGHRDTLRNSGADTVVADLGEVIVRTGDRRMSELPD
ALQTLGLTDDLTARQPAVFFDFDGTLSDIVDDPDSARPVPGATEALQKLATHCPVAILSGRDLADVIKRIGVPGIWYSGS
HGFESTAPDGTHHQNDAAEATIPILEQAATQLRDQLGPIPGVMVEHKRFGVAVHYRNVARDRVNEVAVAVRTAGQRNALR
VTTGREVIELRPDIDWDKGKTLHWVIDRLHHAGTQVGSASLMPICLGDDITDEDAFDAVRHTDVGGIPIVVRHTEDGNRA
TAALFTLDSPMHVSEFTERLARQLSDTQR

Sequences:

>Translated_429_residues
MPVTIDPRRHSAALFDLDAVVTDTPLDSTVTLVRQLQGIGVGTAVFSTNRNSQGVLTATGLDHLFPVHVDGLASGKVTIL
VAAANRLMAQPGRCVVVAVDAAGITAARYGGFALVLGLVIGEDQTGHRDTLRNSGADTVVADLGEVIVRTGDRRMSELPD
ALQTLGLTDDLTARQPAVFFDFDGTLSDIVDDPDSARPVPGATEALQKLATHCPVAILSGRDLADVIKRIGVPGIWYSGS
HGFESTAPDGTHHQNDAAEATIPILEQAATQLRDQLGPIPGVMVEHKRFGVAVHYRNVARDRVNEVAVAVRTAGQRNALR
VTTGREVIELRPDIDWDKGKTLHWVIDRLHHAGTQVGSASLMPICLGDDITDEDAFDAVRHTDVGGIPIVVRHTEDGNRA
TAALFTLDSPMHVSEFTERLARQLSDTQR
>Mature_428_residues
PVTIDPRRHSAALFDLDAVVTDTPLDSTVTLVRQLQGIGVGTAVFSTNRNSQGVLTATGLDHLFPVHVDGLASGKVTILV
AAANRLMAQPGRCVVVAVDAAGITAARYGGFALVLGLVIGEDQTGHRDTLRNSGADTVVADLGEVIVRTGDRRMSELPDA
LQTLGLTDDLTARQPAVFFDFDGTLSDIVDDPDSARPVPGATEALQKLATHCPVAILSGRDLADVIKRIGVPGIWYSGSH
GFESTAPDGTHHQNDAAEATIPILEQAATQLRDQLGPIPGVMVEHKRFGVAVHYRNVARDRVNEVAVAVRTAGQRNALRV
TTGREVIELRPDIDWDKGKTLHWVIDRLHHAGTQVGSASLMPICLGDDITDEDAFDAVRHTDVGGIPIVVRHTEDGNRAT
AALFTLDSPMHVSEFTERLARQLSDTQR

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family

Homologues:

Organism=Escherichia coli, GI1788207, Length=247, Percent_Identity=27.5303643724696, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI221473368, Length=237, Percent_Identity=31.6455696202532, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20129307, Length=237, Percent_Identity=31.6455696202532, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582490, Length=237, Percent_Identity=31.6455696202532, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19920676, Length=230, Percent_Identity=28.695652173913, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI20129309, Length=208, Percent_Identity=25, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTSB_MYCLE (Q49734)

Other databases:

- EMBL:   Z97179
- EMBL:   U00015
- EMBL:   AL583918
- PIR:   S72829
- RefSeq:   NP_301392.1
- ProteinModelPortal:   Q49734
- EnsemblBacteria:   EBMYCT00000029170
- GeneID:   909153
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0414
- NMPDR:   fig|272631.1.peg.264
- Leproma:   ML0414
- GeneTree:   EBGT00050000017221
- HOGENOM:   HBG686849
- OMA:   ATHYRNA
- ProtClustDB:   CLSK792454
- BioCyc:   MLEP272631:ML0414-MONOMER
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01484
- TIGRFAMs:   TIGR00685

Pfam domain/function: PF02358 Trehalose_PPase; SSF56784 SSF56784

EC number: =3.1.3.12

Molecular weight: Translated: 45888; Mature: 45757

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTIDPRRHSAALFDLDAVVTDTPLDSTVTLVRQLQGIGVGTAVFSTNRNSQGVLTATG
CCCCCCCCCCCEEEEEEHHHEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECC
LDHLFPVHVDGLASGKVTILVAAANRLMAQPGRCVVVAVDAAGITAARYGGFALVLGLVI
CCCCCEEEECCCCCCCEEEEEEEHHHHHCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHC
GEDQTGHRDTLRNSGADTVVADLGEVIVRTGDRRMSELPDALQTLGLTDDLTARQPAVFF
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
DFDGTLSDIVDDPDSARPVPGATEALQKLATHCPVAILSGRDLADVIKRIGVPGIWYSGS
ECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHCCCCCEEECCC
HGFESTAPDGTHHQNDAAEATIPILEQAATQLRDQLGPIPGVMVEHKRFGVAVHYRNVAR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCEEEEEEHHHHH
DRVNEVAVAVRTAGQRNALRVTTGREVIELRPDIDWDKGKTLHWVIDRLHHAGTQVGSAS
HHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
LMPICLGDDITDEDAFDAVRHTDVGGIPIVVRHTEDGNRATAALFTLDSPMHVSEFTERL
EEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHH
ARQLSDTQR
HHHHHHCCC
>Mature Secondary Structure 
PVTIDPRRHSAALFDLDAVVTDTPLDSTVTLVRQLQGIGVGTAVFSTNRNSQGVLTATG
CCCCCCCCCCEEEEEEHHHEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECC
LDHLFPVHVDGLASGKVTILVAAANRLMAQPGRCVVVAVDAAGITAARYGGFALVLGLVI
CCCCCEEEECCCCCCCEEEEEEEHHHHHCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHC
GEDQTGHRDTLRNSGADTVVADLGEVIVRTGDRRMSELPDALQTLGLTDDLTARQPAVFF
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
DFDGTLSDIVDDPDSARPVPGATEALQKLATHCPVAILSGRDLADVIKRIGVPGIWYSGS
ECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHCCCCCEEECCC
HGFESTAPDGTHHQNDAAEATIPILEQAATQLRDQLGPIPGVMVEHKRFGVAVHYRNVAR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCEEEEEEHHHHH
DRVNEVAVAVRTAGQRNALRVTTGREVIELRPDIDWDKGKTLHWVIDRLHHAGTQVGSAS
HHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
LMPICLGDDITDEDAFDAVRHTDVGGIPIVVRHTEDGNRATAALFTLDSPMHVSEFTERL
EEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHH
ARQLSDTQR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8446027; 11234002