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The map label for this gene is algU [H]

Identifier: 220918414

GI number: 220918414

Start: 3696483

End: 3697487

Strand: Direct

Name: algU [H]

Synonym: A2cp1_3318

Alternate gene names: 220918414

Gene position: 3696483-3697487 (Clockwise)

Preceding gene: 220918413

Following gene: 220918418

Centisome position: 73.5

GC content: 77.01

Gene sequence:

>1005_bases
ATGGGGGCCGCGCTGGCGCTTCGCACCGGCCTGGTCCTCCGCTATGACCTCGGTCGCATGACCACGGTCGATCAGCTGGA
GACGCACCGGGCGGCGCTCACCGGTCACTGCTACCGGATGCTGGGCTCCGCCGCCGAGGCGGACGACGCGGTCCAGGAGA
CGCTGCTGCGCGCCTGGCGCAGCCTCGAGCGCTTCGAGGGACGCTCGTCGCTCCGCACCTGGCTGTACCGGATCGCCACC
CGGGTGTGCCTCGACGCGCTGGAGGACCGGGCGCGGCGGGCCCGCCCGGTGGATCTCGGGCCGGCCGGCACCGCGGCGGA
CGCGCTCGAGGCGCTCCCTGGGGAGCGCTGGATCGAGCCCATCCCCGACGCGCAGGCGATCCCGGCCGACGTCGATCCCG
CCGAGCAGGTGCTGCTGCGCGAGAGCATCCGGCTCGCGTTCGTGGCCGCGCTCCAGCAGCTGCCGCCGCGCCAGCGGGCC
GCGCTGCTCCTGGCGGACGTGCTGGGCTGGTCGGCCGCGGAGATCGCCGGCGACCTCGACACGTCCGTGGCGGCCGTGAA
CAGCATGCTGCAGCGGGCCCGCGCGACGCTGCCGGCGCGGGATCCCTCCGGCCCCGGCGCCGCGCTGTCGCAGGCCGAGG
CCGTGCTGCTCGAGCGCTACGTGGACGCCTTCGAGCGCTACGACGTGGCGGCGCTCACCGCGACGCTCCGCGAGGACGCC
TGCTTCTCCATGCCGCCGTTCTCGCTCTGGCTGCGGGGGCGCGAGGCGGTGGCGGCCTGGCTCACGGGCCCGGGCGCCGG
CTGCCGCGGCTCGCGCCTGGTGCCCACCGCCGCGAGCGGCCTGCCCGCGTTCGCGCAGTACCGGCCCGCCGGCCCCGGCG
TCCACCGCCCCTGGGCGCTCCTCGTGCTGGAGCCGGCGCCCGGCGGGATCGCCGCCTGGACCGCGTTCCTCGACACGGCC
ACCCTGTTCCCGCGCTTCGGCCTCCCGTCCGAGCTCACCTCGTAG

Upstream 100 bases:

>100_bases
CGGGCAGGTGCGCGGCGTCCGCCGGATCGACAACCGGCTCGAGGTGCACGACGCGCCGGGCAAGCACCCGGGCCTGCAGA
GCTAGGCGGGTGGCGCCCGC

Downstream 100 bases:

>100_bases
CCGCGCTCAGGGCCGGCTCGGCGATGGCCCGCAGGTCCACCTCCGGGCACCCCACGGCCACCCCGTAGCGCTCGGCCCAG
CCGCGCGCCTCGGCCAGCGA

Product: RNA polymerase factor sigma-70

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MGAALALRTGLVLRYDLGRMTTVDQLETHRAALTGHCYRMLGSAAEADDAVQETLLRAWRSLERFEGRSSLRTWLYRIAT
RVCLDALEDRARRARPVDLGPAGTAADALEALPGERWIEPIPDAQAIPADVDPAEQVLLRESIRLAFVAALQQLPPRQRA
ALLLADVLGWSAAEIAGDLDTSVAAVNSMLQRARATLPARDPSGPGAALSQAEAVLLERYVDAFERYDVAALTATLREDA
CFSMPPFSLWLRGREAVAAWLTGPGAGCRGSRLVPTAASGLPAFAQYRPAGPGVHRPWALLVLEPAPGGIAAWTAFLDTA
TLFPRFGLPSELTS

Sequences:

>Translated_334_residues
MGAALALRTGLVLRYDLGRMTTVDQLETHRAALTGHCYRMLGSAAEADDAVQETLLRAWRSLERFEGRSSLRTWLYRIAT
RVCLDALEDRARRARPVDLGPAGTAADALEALPGERWIEPIPDAQAIPADVDPAEQVLLRESIRLAFVAALQQLPPRQRA
ALLLADVLGWSAAEIAGDLDTSVAAVNSMLQRARATLPARDPSGPGAALSQAEAVLLERYVDAFERYDVAALTATLREDA
CFSMPPFSLWLRGREAVAAWLTGPGAGCRGSRLVPTAASGLPAFAQYRPAGPGVHRPWALLVLEPAPGGIAAWTAFLDTA
TLFPRFGLPSELTS
>Mature_333_residues
GAALALRTGLVLRYDLGRMTTVDQLETHRAALTGHCYRMLGSAAEADDAVQETLLRAWRSLERFEGRSSLRTWLYRIATR
VCLDALEDRARRARPVDLGPAGTAADALEALPGERWIEPIPDAQAIPADVDPAEQVLLRESIRLAFVAALQQLPPRQRAA
LLLADVLGWSAAEIAGDLDTSVAAVNSMLQRARATLPARDPSGPGAALSQAEAVLLERYVDAFERYDVAALTATLREDAC
FSMPPFSLWLRGREAVAAWLTGPGAGCRGSRLVPTAASGLPAFAQYRPAGPGVHRPWALLVLEPAPGGIAAWTAFLDTAT
LFPRFGLPSELTS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=152, Percent_Identity=32.2368421052632, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 35882; Mature: 35751

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAALALRTGLVLRYDLGRMTTVDQLETHRAALTGHCYRMLGSAAEADDAVQETLLRAWR
CCCHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SLERFEGRSSLRTWLYRIATRVCLDALEDRARRARPVDLGPAGTAADALEALPGERWIEP
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCC
IPDAQAIPADVDPAEQVLLRESIRLAFVAALQQLPPRQRAALLLADVLGWSAAEIAGDLD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCHH
TSVAAVNSMLQRARATLPARDPSGPGAALSQAEAVLLERYVDAFERYDVAALTATLREDA
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CFSMPPFSLWLRGREAVAAWLTGPGAGCRGSRLVPTAASGLPAFAQYRPAGPGVHRPWAL
CCCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEE
LVLEPAPGGIAAWTAFLDTATLFPRFGLPSELTS
EEECCCCCCHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
GAALALRTGLVLRYDLGRMTTVDQLETHRAALTGHCYRMLGSAAEADDAVQETLLRAWR
CCHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SLERFEGRSSLRTWLYRIATRVCLDALEDRARRARPVDLGPAGTAADALEALPGERWIEP
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCC
IPDAQAIPADVDPAEQVLLRESIRLAFVAALQQLPPRQRAALLLADVLGWSAAEIAGDLD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCHH
TSVAAVNSMLQRARATLPARDPSGPGAALSQAEAVLLERYVDAFERYDVAALTATLREDA
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CFSMPPFSLWLRGREAVAAWLTGPGAGCRGSRLVPTAASGLPAFAQYRPAGPGVHRPWAL
CCCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEE
LVLEPAPGGIAAWTAFLDTATLFPRFGLPSELTS
EEECCCCCCHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]