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The map label for this gene is dpnA [H]

Identifier: 220918133

GI number: 220918133

Start: 3358519

End: 3359379

Strand: Direct

Name: dpnA [H]

Synonym: A2cp1_3036

Alternate gene names: 220918133

Gene position: 3358519-3359379 (Clockwise)

Preceding gene: 220918128

Following gene: 220918134

Centisome position: 66.78

GC content: 72.94

Gene sequence:

>861_bases
ATGGCGACTCGGCTCGAGCTCCTTCACGAGTGGAACGACCTGCCCGGCGTGGCCGAGCGAGGGCGCGGCTGGGCGCTCGT
CCAGGGCGACTGCGTCGAGGCGCTGGAGCGCCTCCCCCCGCACTCGGTGGACGTCGCCTTCGCCGACCCGCCCTACATGC
TCTCGAACGGCGGCAGCACCTGCCAGGGCGGGCGCCGCACCTCCGTGAACAAGGGGAGCTGGGACGCCTCCGGCGGCTTC
GCCGCGGACCACGCGTTCCAGGCGCGCTGGCTCCAGGCGGTCCGCAAGGTGCTGAAGCCGTCCGGCACGCTGTGGGTCTC
GGGCACGCAGCACGTCATCTTCTCCATCGGCTACGCGATGCAGGAGCTGGGCTTCCACCTGCTCAACACCGTCACCTGGT
ACAAGCCCAACGCCTCACCCAACCTGGCCTGCCGCTTCTTCACGCACTCGACCGAGATCCTGCTCTGGGCGAGCCCGATG
AAGGCGCGCCCGCTGGCGCACCGGTTCAACTACCGCGCGATGAAGACCGCCAACGGCGGCAAGCAGATGCGCGACCTGTG
GGAGATCGCCGACCGCCCGGCGCCGGGCGGCGACCAGGTGGTGTGGTCGGTGCCCACGCCCGGGCCGCGCGAGAAGGTGC
ACGGCCGCCACCCCACGCAGAAGCCGCTCGCGCTGCTCGAGCGCGTGCTCGCCGCCAGCGCCGCGCCCGGCGACCTGGTG
CTGGACCCGTTCTCGGGCTCCGCGACCACCGGCGTCGCCGCGCTCCGTGCGGGCTGCCGCTTCCTCGGGCTGGAGCGCGA
TCCCGGGTACGTGGACCTCGCCGCCCGCCGCCTCCGCGCCACCACGATCGATCCGGAGTGA

Upstream 100 bases:

>100_bases
CTTAGCACCGGACGCGTCCCGCCGCCGGGGTGCCCTCTCGTTCTCTGGCAGTGCGGCCGAAAACTGGCGATATCGATCGG
CCCCTCTACGATGCGGCGAT

Downstream 100 bases:

>100_bases
CGCACCCGCTGTGAGCAGCCCGGGCGGCGCGCGAGCAGCCGCGCACGCGTGCGCCCGTCGGGCGACTGCCTTGTTCCGGG
CGAGGTGCGTACATTCGTCG

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.DpnIIB; Adenine-specific methyltransferase DpnIIB; M.DpnII 2 [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MATRLELLHEWNDLPGVAERGRGWALVQGDCVEALERLPPHSVDVAFADPPYMLSNGGSTCQGGRRTSVNKGSWDASGGF
AADHAFQARWLQAVRKVLKPSGTLWVSGTQHVIFSIGYAMQELGFHLLNTVTWYKPNASPNLACRFFTHSTEILLWASPM
KARPLAHRFNYRAMKTANGGKQMRDLWEIADRPAPGGDQVVWSVPTPGPREKVHGRHPTQKPLALLERVLAASAAPGDLV
LDPFSGSATTGVAALRAGCRFLGLERDPGYVDLAARRLRATTIDPE

Sequences:

>Translated_286_residues
MATRLELLHEWNDLPGVAERGRGWALVQGDCVEALERLPPHSVDVAFADPPYMLSNGGSTCQGGRRTSVNKGSWDASGGF
AADHAFQARWLQAVRKVLKPSGTLWVSGTQHVIFSIGYAMQELGFHLLNTVTWYKPNASPNLACRFFTHSTEILLWASPM
KARPLAHRFNYRAMKTANGGKQMRDLWEIADRPAPGGDQVVWSVPTPGPREKVHGRHPTQKPLALLERVLAASAAPGDLV
LDPFSGSATTGVAALRAGCRFLGLERDPGYVDLAARRLRATTIDPE
>Mature_285_residues
ATRLELLHEWNDLPGVAERGRGWALVQGDCVEALERLPPHSVDVAFADPPYMLSNGGSTCQGGRRTSVNKGSWDASGGFA
ADHAFQARWLQAVRKVLKPSGTLWVSGTQHVIFSIGYAMQELGFHLLNTVTWYKPNASPNLACRFFTHSTEILLWASPMK
ARPLAHRFNYRAMKTANGGKQMRDLWEIADRPAPGGDQVVWSVPTPGPREKVHGRHPTQKPLALLERVLAASAAPGDLVL
DPFSGSATTGVAALRAGCRFLGLERDPGYVDLAARRLRATTIDPE

Specific function: Essential for establishment of a previously unmethylated plasmid transformed into the cell as single stranded DNA. Recognizes the single- or double-stranded sequence GATC, causes specific methylation on A-2 on one or both strands (respectively), and prote

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=259, Percent_Identity=29.3436293436293, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31240; Mature: 31109

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRLELLHEWNDLPGVAERGRGWALVQGDCVEALERLPPHSVDVAFADPPYMLSNGGST
CCHHHHHHHHHCCCCCHHHCCCCEEEECCHHHHHHHHCCCCCEEEEECCCCEEECCCCCC
CQGGRRTSVNKGSWDASGGFAADHAFQARWLQAVRKVLKPSGTLWVSGTQHVIFSIGYAM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEHHHHHHH
QELGFHLLNTVTWYKPNASPNLACRFFTHSTEILLWASPMKARPLAHRFNYRAMKTANGG
HHHHHHHHHHEEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCHHHHCCCEEEECCCCH
KQMRDLWEIADRPAPGGDQVVWSVPTPGPREKVHGRHPTQKPLALLERVLAASAAPGDLV
HHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEE
LDPFSGSATTGVAALRAGCRFLGLERDPGYVDLAARRLRATTIDPE
EECCCCCCHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ATRLELLHEWNDLPGVAERGRGWALVQGDCVEALERLPPHSVDVAFADPPYMLSNGGST
CHHHHHHHHHCCCCCHHHCCCCEEEECCHHHHHHHHCCCCCEEEEECCCCEEECCCCCC
CQGGRRTSVNKGSWDASGGFAADHAFQARWLQAVRKVLKPSGTLWVSGTQHVIFSIGYAM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEHHHHHHH
QELGFHLLNTVTWYKPNASPNLACRFFTHSTEILLWASPMKARPLAHRFNYRAMKTANGG
HHHHHHHHHHEEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCHHHHCCCEEEECCCCH
KQMRDLWEIADRPAPGGDQVVWSVPTPGPREKVHGRHPTQKPLALLERVLAASAAPGDLV
HHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEE
LDPFSGSATTGVAALRAGCRFLGLERDPGYVDLAARRLRATTIDPE
EECCCCCCHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019562; 10712690; 2824782 [H]