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The map label for this gene is mazG [H]

Identifier: 220918007

GI number: 220918007

Start: 3220987

End: 3221844

Strand: Direct

Name: mazG [H]

Synonym: A2cp1_2908

Alternate gene names: 220918007

Gene position: 3220987-3221844 (Clockwise)

Preceding gene: 220917999

Following gene: 220918008

Centisome position: 64.04

GC content: 73.19

Gene sequence:

>858_bases
ATGTCGCGCGCCGCCGAGGCCATCGATCGCCTGCTCTCCATCATGGAGCGCCTGCGCGGCCCGGGGGGCTGCCCCTGGGA
CCGCGAGCAGACGCTCCACACGCTGCGCCCGTACGTGCTGGAGGAGACGTACGAGGTCCTCGAGGCCATCGACGCGGGCG
ACGTCCGCGAGCACCGCGAGGAGCTGGGCGACCTGCTGCTGCAGGTCGTCTTCCAGGCGCAGCTCCGTCGCGAGGAGGGC
GCGTTCGAGTTCGCGGACGTGGCGGACGCCATCTCGGACAAGCTCGTCTCGCGCCACCCGCACGTGTTCGGCGACGCGCA
GGTGAAGGACGCCGAGGGCGTGCTGCGGCAGTGGGCGGCGCTGAAGCGCGAGGAGAAGAAGGCGAAGGGCGCCGGCCACA
GCGTGCTGGAGGGCGTGCCGCGCGAGATGCCGGCGCTGGCGCGGGCCGACCGGCTCACCGAGAAGGCGAGCCGCATCGGG
TTCGACTGGCCCGACGCGGGCGGCGCGCGCGAGAAGGTGCGCGAGGAGCTCGCCGAGCTGGACCAGGCGGTGGCGGGCGG
CGACCGCGCGGCCGTCGAGCACGAGCTGGGCGACGCGCTGTTCGCGCTCGCGAACTACGCCCGCAAGCTCGGCGTCCCGC
CGGAGGAGGCGCTCCGCGGGAGCCTGGGCCGATTCGTCGCACGCTTCGAGCACGTCGAGCGCGAGCTGGAGCGCCGCGGC
GTGCCCCACGGCAGCGCCACGCTGGCGGAGATGGACGCGCTCTGGGACGAGGCGAAGGAGCTCGAGCGTCAAGCGAAATC
GCCCGGCACCGCCCCTTCGGCTGGTTCTCAACGGGGTTTGGGGAAAGATGGTGGATAA

Upstream 100 bases:

>100_bases
CGCGGCCAGCACGGCGAACAGGGTCGGGAGGGCGGTACCGCGGGTGGACACGCGAGAACGGGATGCTAGCACGCCCGGTC
CCGCGCTAAGAAGACGAACC

Downstream 100 bases:

>100_bases
GTCGGTGGGCGGGGCGCCTCGACGCAGGGAATTCACCCCATCGGCACGTGGCGCCGCGTTCCGCAGCACCAGAAATCTCG
TGTGATTTCAGCGTGCTCAC

Product: MazG family protein

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSRAAEAIDRLLSIMERLRGPGGCPWDREQTLHTLRPYVLEETYEVLEAIDAGDVREHREELGDLLLQVVFQAQLRREEG
AFEFADVADAISDKLVSRHPHVFGDAQVKDAEGVLRQWAALKREEKKAKGAGHSVLEGVPREMPALARADRLTEKASRIG
FDWPDAGGAREKVREELAELDQAVAGGDRAAVEHELGDALFALANYARKLGVPPEEALRGSLGRFVARFEHVERELERRG
VPHGSATLAEMDALWDEAKELERQAKSPGTAPSAGSQRGLGKDGG

Sequences:

>Translated_285_residues
MSRAAEAIDRLLSIMERLRGPGGCPWDREQTLHTLRPYVLEETYEVLEAIDAGDVREHREELGDLLLQVVFQAQLRREEG
AFEFADVADAISDKLVSRHPHVFGDAQVKDAEGVLRQWAALKREEKKAKGAGHSVLEGVPREMPALARADRLTEKASRIG
FDWPDAGGAREKVREELAELDQAVAGGDRAAVEHELGDALFALANYARKLGVPPEEALRGSLGRFVARFEHVERELERRG
VPHGSATLAEMDALWDEAKELERQAKSPGTAPSAGSQRGLGKDGG
>Mature_284_residues
SRAAEAIDRLLSIMERLRGPGGCPWDREQTLHTLRPYVLEETYEVLEAIDAGDVREHREELGDLLLQVVFQAQLRREEGA
FEFADVADAISDKLVSRHPHVFGDAQVKDAEGVLRQWAALKREEKKAKGAGHSVLEGVPREMPALARADRLTEKASRIGF
DWPDAGGAREKVREELAELDQAVAGGDRAAVEHELGDALFALANYARKLGVPPEEALRGSLGRFVARFEHVERELERRGV
PHGSATLAEMDALWDEAKELERQAKSPGTAPSAGSQRGLGKDGG

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=260, Percent_Identity=48.4615384615385, Blast_Score=234, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 31323; Mature: 31192

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00268 CECROPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAAEAIDRLLSIMERLRGPGGCPWDREQTLHTLRPYVLEETYEVLEAIDAGDVREHRE
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ELGDLLLQVVFQAQLRREEGAFEFADVADAISDKLVSRHPHVFGDAQVKDAEGVLRQWAA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LKREEKKAKGAGHSVLEGVPREMPALARADRLTEKASRIGFDWPDAGGAREKVREELAEL
HHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DQAVAGGDRAAVEHELGDALFALANYARKLGVPPEEALRGSLGRFVARFEHVERELERRG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPHGSATLAEMDALWDEAKELERQAKSPGTAPSAGSQRGLGKDGG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRAAEAIDRLLSIMERLRGPGGCPWDREQTLHTLRPYVLEETYEVLEAIDAGDVREHRE
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ELGDLLLQVVFQAQLRREEGAFEFADVADAISDKLVSRHPHVFGDAQVKDAEGVLRQWAA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LKREEKKAKGAGHSVLEGVPREMPALARADRLTEKASRIGFDWPDAGGAREKVREELAEL
HHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DQAVAGGDRAAVEHELGDALFALANYARKLGVPPEEALRGSLGRFVARFEHVERELERRG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPHGSATLAEMDALWDEAKELERQAKSPGTAPSAGSQRGLGKDGG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]