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The map label for this gene is topA [H]

Identifier: 220917984

GI number: 220917984

Start: 3186497

End: 3188992

Strand: Reverse

Name: topA [H]

Synonym: A2cp1_2885

Alternate gene names: 220917984

Gene position: 3188992-3186497 (Counterclockwise)

Preceding gene: 220917985

Following gene: 220917982

Centisome position: 63.41

GC content: 69.47

Gene sequence:

>2496_bases
ATGGCGAGAGAGCCGAAGACGGGCGCGGTCGCGGTCAAGAAGAAGAAGGCCGCGGCGGCCCCGAAGGAAGCGACGGCCGT
CGCCGAGGCGGAAGCCCCGAAGACCCCCCGCCGCCCAGCGCGGAAGACCTCCACCGGGACCAGCCTGGTGGTGGTGGAGT
CGCCCGCGAAGGCGAAGACCATCAAGAAGTACCTGGGGCGGTCGTTCGACGTGAAGGCGTCGGTCGGTCACGTGAAGGAT
CTGCCCAAGTCGAAGATCGGCGTGGACGTCGAGAAGGGCTTCCTCCCCGAGTACGACGTCATCAAGGGGAAGGCGAAGGT
CCTCTCGGAGATCAAGCGCGCGGCGCGGACCGCCGACCACGTCTTCCTCGCGACCGACCCCGATCGCGAGGGCGAGGCCA
TCGCCTGGCACATCTCCGAGGAGCTCGGCGCCGAGGGCGGCGACGCCCGGGTGCGCCGGGTGCTCTTCAACGAGATCACC
AAGACGGCCATCCAGAAGGCCATCGAGAAGCCGCTCGACCTCGACCGCAAGAAGTTCGACTCGCAGCAGGCCCGCCGGAT
CCTCGATCGCCTGGTCGGCTACCAGATCAGCCCCATCCTCTGGAAGAAGGTCCGCCGCGGCCTGTCCGCCGGCCGGGTGC
AGTCGGTGGCGGTGCGGCTGGTGGTGGAGCGCGAGCGCGAGATCGAGGCGTTCGTGCCGGTGGAGTACTGGTCGCTCGAC
GCCGACCTCGCCGCGCGCGTCCCGCCCGACTTCCGCGCCAAGATCTTCAAGGTGGACGGCCAGAAGGCCGACCTGAAGCA
GGGCGAGGAGACCCAGGCGCTGGTGAAGGACCTGGAGGGCGCGCGCTACCTGGTCGCGTCGGTGGACCGCAAGGAGCGCA
AGCGCAACCCGCCGCCGCCCTTCACCACCGCGAAGCTGCAGCAGGAGGCGGCGAACCGCCTCGGCTTCAGCCCGAAGAAG
ACCATGACGCTCGCGCAGAAGCTCTACGAGGGCGTCGAGCTCGGCGACGAGGGGGCCATCGGCCTCATCACGTACATGCG
CACCGACTCGGTGCGGCTCGCCACCGAGGCGGTGGACGCGGTGCGCGCCCACATCCAGCAGGCGTACGGGACGGACCACC
TGCCGGAGCAGGCCAACGTCTACAAGACGAAGGCGAAGGCCGCCCAGGAGGCGCACGAGGCGGTGCGCCCCACCTCGATG
GACTGGACGCCGGACCGCGTGGCGCCGTTCTTCGACCAGATGGGCGAGCGCGACATGTACCGGCTCTACGAGCTCATCTG
GAACCGGTTCGTGGCGTGCCAGATGGTGCCGGCGATCTACGACCAGACCTCGGCCGACATCGCCGCCGGCCGCGCGGTGT
TCCGGGCCACCGGGTCGATCCTCAAGTTCCCCGGCTACCTCGCCGTCTACGGGGCGAAGCCGCCCGAGGAGGAGGCCGGG
GCCGAGGAGGAGAAGACGAACGGCGACGCCGCCGAGCCGGTGGCCCGCGCCGAGGACCGCCAGCTCCCGCCGCTCGAGGC
CGGGATGGAGCTGCGCCTGCTGAAGCTGCTGCCCGAGCAGCACTTCACGCAGCCGCCGCCGCGGTTCAACGAGTCGTCGC
TGGTGAAGGAGATGGAGGAGCGCGGCATCGGCCGGCCCTCCACGTACGCGGCCATCCTCGACACGATCCAGGAGAAGGGC
TACGTCGAGAAGCTCGAGAAGCAGTTCAAGCCGACCCACCTCGGCGTCCTGGTGACCGACGAGCTGGTGCGGGCGTTCCC
GCGCGAGATGGACATCGCGTTCACCGCCGGGATGGAGGAGAAGCTCGACGGCATCGAGGAGGGCGCCGCCGGCTGGCAGC
AGGTGCTGGAGGAGTTCTACTCGAAGTTCAAGGAGGACCTCGCCAAGGCGGACGAGGCCATGCGCAACGTGAAGGCGCAG
GCCATCGAGACCGACATCACCTGCGAGAAGTGCGGCCAGCACAAGATGGTCATCAAGTGGGGCCGCAACGGCGAGTTCCT
CGCCTGCCCCGGGTACCCGGAGTGCCGGAACACCATGAACTTCCGGCGCGAGGACGGGAACATCGTCCCGGAGAAGGAGG
AGGACGTTCCGGTCGAGGACAAGTGCCCGGAGTGCGGCGCGGACATGGTGATGAAGCGCGGCCGCTTCGGCCGGTTCCTC
GCCTGCACGCGCTACCCGGACTGCAAGGGCACCAAGCCCGTCTCGATCGGCGTGACCTGCCCGAAGGGCTGCGGCGGCTA
CATCAGCGAGAAGCGCTCCCGCCGCGGCAAGACGTTCTACGGCTGCTCGTCGTATCCCAAGTGCGACTTCGTGGCCTGGG
ACCGCCCGCGCAACGAGACCTGCCCGCAGTGCGGCAGCGCCTACCTGCTGGACAAGTACTCGAAGAAGACCGGCCCGTTC
GTCGCGTGCCCGAACAAGGAGTGCGGCTACCGGCGCGAGGCCGAGCCGCGCGAGGGCGGCGCGGAGGCGGCCCGGGCGGA
GCCGGTCAAGGCGTAG

Upstream 100 bases:

>100_bases
AGCCGCGCTGGCGGGGCTCCTGACGCTCGAGCTGGAAGGTCTGTGCGAGCAGCGGCCGGGCCATTACTTTCTGCGCCGCA
GTCGAGAGGGGATCTAGAGG

Downstream 100 bases:

>100_bases
CGATCCGCGACCGCCGCGGACAGGCGAAGGCCGGCGCCGGGGCTCCCCGGGCCGGCCTTCGTCGCGCCCGGGACACGGGC
TACATCGTGGGCGGCTCGCC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 831; Mature: 830

Protein sequence:

>831_residues
MAREPKTGAVAVKKKKAAAAPKEATAVAEAEAPKTPRRPARKTSTGTSLVVVESPAKAKTIKKYLGRSFDVKASVGHVKD
LPKSKIGVDVEKGFLPEYDVIKGKAKVLSEIKRAARTADHVFLATDPDREGEAIAWHISEELGAEGGDARVRRVLFNEIT
KTAIQKAIEKPLDLDRKKFDSQQARRILDRLVGYQISPILWKKVRRGLSAGRVQSVAVRLVVEREREIEAFVPVEYWSLD
ADLAARVPPDFRAKIFKVDGQKADLKQGEETQALVKDLEGARYLVASVDRKERKRNPPPPFTTAKLQQEAANRLGFSPKK
TMTLAQKLYEGVELGDEGAIGLITYMRTDSVRLATEAVDAVRAHIQQAYGTDHLPEQANVYKTKAKAAQEAHEAVRPTSM
DWTPDRVAPFFDQMGERDMYRLYELIWNRFVACQMVPAIYDQTSADIAAGRAVFRATGSILKFPGYLAVYGAKPPEEEAG
AEEEKTNGDAAEPVARAEDRQLPPLEAGMELRLLKLLPEQHFTQPPPRFNESSLVKEMEERGIGRPSTYAAILDTIQEKG
YVEKLEKQFKPTHLGVLVTDELVRAFPREMDIAFTAGMEEKLDGIEEGAAGWQQVLEEFYSKFKEDLAKADEAMRNVKAQ
AIETDITCEKCGQHKMVIKWGRNGEFLACPGYPECRNTMNFRREDGNIVPEKEEDVPVEDKCPECGADMVMKRGRFGRFL
ACTRYPDCKGTKPVSIGVTCPKGCGGYISEKRSRRGKTFYGCSSYPKCDFVAWDRPRNETCPQCGSAYLLDKYSKKTGPF
VACPNKECGYRREAEPREGGAEAARAEPVKA

Sequences:

>Translated_831_residues
MAREPKTGAVAVKKKKAAAAPKEATAVAEAEAPKTPRRPARKTSTGTSLVVVESPAKAKTIKKYLGRSFDVKASVGHVKD
LPKSKIGVDVEKGFLPEYDVIKGKAKVLSEIKRAARTADHVFLATDPDREGEAIAWHISEELGAEGGDARVRRVLFNEIT
KTAIQKAIEKPLDLDRKKFDSQQARRILDRLVGYQISPILWKKVRRGLSAGRVQSVAVRLVVEREREIEAFVPVEYWSLD
ADLAARVPPDFRAKIFKVDGQKADLKQGEETQALVKDLEGARYLVASVDRKERKRNPPPPFTTAKLQQEAANRLGFSPKK
TMTLAQKLYEGVELGDEGAIGLITYMRTDSVRLATEAVDAVRAHIQQAYGTDHLPEQANVYKTKAKAAQEAHEAVRPTSM
DWTPDRVAPFFDQMGERDMYRLYELIWNRFVACQMVPAIYDQTSADIAAGRAVFRATGSILKFPGYLAVYGAKPPEEEAG
AEEEKTNGDAAEPVARAEDRQLPPLEAGMELRLLKLLPEQHFTQPPPRFNESSLVKEMEERGIGRPSTYAAILDTIQEKG
YVEKLEKQFKPTHLGVLVTDELVRAFPREMDIAFTAGMEEKLDGIEEGAAGWQQVLEEFYSKFKEDLAKADEAMRNVKAQ
AIETDITCEKCGQHKMVIKWGRNGEFLACPGYPECRNTMNFRREDGNIVPEKEEDVPVEDKCPECGADMVMKRGRFGRFL
ACTRYPDCKGTKPVSIGVTCPKGCGGYISEKRSRRGKTFYGCSSYPKCDFVAWDRPRNETCPQCGSAYLLDKYSKKTGPF
VACPNKECGYRREAEPREGGAEAARAEPVKA
>Mature_830_residues
AREPKTGAVAVKKKKAAAAPKEATAVAEAEAPKTPRRPARKTSTGTSLVVVESPAKAKTIKKYLGRSFDVKASVGHVKDL
PKSKIGVDVEKGFLPEYDVIKGKAKVLSEIKRAARTADHVFLATDPDREGEAIAWHISEELGAEGGDARVRRVLFNEITK
TAIQKAIEKPLDLDRKKFDSQQARRILDRLVGYQISPILWKKVRRGLSAGRVQSVAVRLVVEREREIEAFVPVEYWSLDA
DLAARVPPDFRAKIFKVDGQKADLKQGEETQALVKDLEGARYLVASVDRKERKRNPPPPFTTAKLQQEAANRLGFSPKKT
MTLAQKLYEGVELGDEGAIGLITYMRTDSVRLATEAVDAVRAHIQQAYGTDHLPEQANVYKTKAKAAQEAHEAVRPTSMD
WTPDRVAPFFDQMGERDMYRLYELIWNRFVACQMVPAIYDQTSADIAAGRAVFRATGSILKFPGYLAVYGAKPPEEEAGA
EEEKTNGDAAEPVARAEDRQLPPLEAGMELRLLKLLPEQHFTQPPPRFNESSLVKEMEERGIGRPSTYAAILDTIQEKGY
VEKLEKQFKPTHLGVLVTDELVRAFPREMDIAFTAGMEEKLDGIEEGAAGWQQVLEEFYSKFKEDLAKADEAMRNVKAQA
IETDITCEKCGQHKMVIKWGRNGEFLACPGYPECRNTMNFRREDGNIVPEKEEDVPVEDKCPECGADMVMKRGRFGRFLA
CTRYPDCKGTKPVSIGVTCPKGCGGYISEKRSRRGKTFYGCSSYPKCDFVAWDRPRNETCPQCGSAYLLDKYSKKTGPFV
ACPNKECGYRREAEPREGGAEAARAEPVKA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=607, Percent_Identity=25.3706754530478, Blast_Score=137, Evalue=5e-32,
Organism=Homo sapiens, GI4507635, Length=627, Percent_Identity=23.9234449760766, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1787529, Length=833, Percent_Identity=40.8163265306122, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI48994931, Length=179, Percent_Identity=37.4301675977654, Blast_Score=118, Evalue=2e-27,
Organism=Escherichia coli, GI1788061, Length=575, Percent_Identity=24.695652173913, Blast_Score=106, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=769, Percent_Identity=22.8868660598179, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17555378, Length=576, Percent_Identity=23.9583333333333, Blast_Score=105, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323263, Length=606, Percent_Identity=25.9075907590759, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24585251, Length=683, Percent_Identity=24.0117130307467, Blast_Score=155, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24640096, Length=535, Percent_Identity=23.9252336448598, Blast_Score=117, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 92569; Mature: 92437

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREPKTGAVAVKKKKAAAAPKEATAVAEAEAPKTPRRPARKTSTGTSLVVVESPAKAKT
CCCCCCCCCEEEEHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHHH
IKKYLGRSFDVKASVGHVKDLPKSKIGVDVEKGFLPEYDVIKGKAKVLSEIKRAARTADH
HHHHHCCCCCCCCCCCHHHCCCCHHCCCCHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCE
VFLATDPDREGEAIAWHISEELGAEGGDARVRRVLFNEITKTAIQKAIEKPLDLDRKKFD
EEEECCCCCCCCEEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
SQQARRILDRLVGYQISPILWKKVRRGLSAGRVQSVAVRLVVEREREIEAFVPVEYWSLD
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEECCHHHEECC
ADLAARVPPDFRAKIFKVDGQKADLKQGEETQALVKDLEGARYLVASVDRKERKRNPPPP
CHHHHCCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHCCCEEHHHHCCHHHHCCCCCCC
FTTAKLQQEAANRLGFSPKKTMTLAQKLYEGVELGDEGAIGLITYMRTDSVRLATEAVDA
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHH
VRAHIQQAYGTDHLPEQANVYKTKAKAAQEAHEAVRPTSMDWTPDRVAPFFDQMGERDMY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
RLYELIWNRFVACQMVPAIYDQTSADIAAGRAVFRATGSILKFPGYLAVYGAKPPEEEAG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCHHHCC
AEEEKTNGDAAEPVARAEDRQLPPLEAGMELRLLKLLPEQHFTQPPPRFNESSLVKEMEE
CCHHHCCCCHHCHHHHHCCCCCCCHHCCHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHH
RGIGRPSTYAAILDTIQEKGYVEKLEKQFKPTHLGVLVTDELVRAFPREMDIAFTAGMEE
CCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEHHHHHHHCCHHCCEEEECCCHH
KLDGIEEGAAGWQQVLEEFYSKFKEDLAKADEAMRNVKAQAIETDITCEKCGQHKMVIKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEE
GRNGEFLACPGYPECRNTMNFRREDGNIVPEKEEDVPVEDKCPECGADMVMKRGRFGRFL
CCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEE
ACTRYPDCKGTKPVSIGVTCPKGCGGYISEKRSRRGKTFYGCSSYPKCDFVAWDRPRNET
EEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCEEECCCCCCCC
CPQCGSAYLLDKYSKKTGPFVACPNKECGYRREAEPREGGAEAARAEPVKA
CCHHCCHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
AREPKTGAVAVKKKKAAAAPKEATAVAEAEAPKTPRRPARKTSTGTSLVVVESPAKAKT
CCCCCCCCEEEEHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHHH
IKKYLGRSFDVKASVGHVKDLPKSKIGVDVEKGFLPEYDVIKGKAKVLSEIKRAARTADH
HHHHHCCCCCCCCCCCHHHCCCCHHCCCCHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCE
VFLATDPDREGEAIAWHISEELGAEGGDARVRRVLFNEITKTAIQKAIEKPLDLDRKKFD
EEEECCCCCCCCEEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
SQQARRILDRLVGYQISPILWKKVRRGLSAGRVQSVAVRLVVEREREIEAFVPVEYWSLD
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEECCHHHEECC
ADLAARVPPDFRAKIFKVDGQKADLKQGEETQALVKDLEGARYLVASVDRKERKRNPPPP
CHHHHCCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHCCCEEHHHHCCHHHHCCCCCCC
FTTAKLQQEAANRLGFSPKKTMTLAQKLYEGVELGDEGAIGLITYMRTDSVRLATEAVDA
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHH
VRAHIQQAYGTDHLPEQANVYKTKAKAAQEAHEAVRPTSMDWTPDRVAPFFDQMGERDMY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
RLYELIWNRFVACQMVPAIYDQTSADIAAGRAVFRATGSILKFPGYLAVYGAKPPEEEAG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCHHHCC
AEEEKTNGDAAEPVARAEDRQLPPLEAGMELRLLKLLPEQHFTQPPPRFNESSLVKEMEE
CCHHHCCCCHHCHHHHHCCCCCCCHHCCHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHH
RGIGRPSTYAAILDTIQEKGYVEKLEKQFKPTHLGVLVTDELVRAFPREMDIAFTAGMEE
CCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEHHHHHHHCCHHCCEEEECCCHH
KLDGIEEGAAGWQQVLEEFYSKFKEDLAKADEAMRNVKAQAIETDITCEKCGQHKMVIKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEE
GRNGEFLACPGYPECRNTMNFRREDGNIVPEKEEDVPVEDKCPECGADMVMKRGRFGRFL
CCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEE
ACTRYPDCKGTKPVSIGVTCPKGCGGYISEKRSRRGKTFYGCSSYPKCDFVAWDRPRNET
EEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCEEECCCCCCCC
CPQCGSAYLLDKYSKKTGPFVACPNKECGYRREAEPREGGAEAARAEPVKA
CCHHCCHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]