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The map label for this gene is greB [H]

Identifier: 220917962

GI number: 220917962

Start: 3166833

End: 3167324

Strand: Direct

Name: greB [H]

Synonym: A2cp1_2863

Alternate gene names: 220917962

Gene position: 3166833-3167324 (Clockwise)

Preceding gene: 220917960

Following gene: 220917963

Centisome position: 62.97

GC content: 72.76

Gene sequence:

>492_bases
ATGTCCAGGACGCCCCGATACATCACGCCCGAAGGGTTCCGGCGCCTCGCCGCCGAGCACGCCCGCATCTGGACCGAGCT
GCGGCCGCGCATCGTGGCCGAGGTCGAGGCCGCCGCCGCGCTGGGCGACCGCAGCGAGAACGCCGAGTACATCTACGGCA
AGAAGAAGCTGCGGGAGCTGGATCGCCGCCTGCGCTTCCTCTCGGAGAAGATGGACTCGCTCTCGGTGGTCGAGCCGCGG
GCCCACCCGGACGGCCGGGCGTTCTTCGGCGCCTGGGTGACCGTCGAGCAGGAGGACGGCCAGGCGCGGAGGGTCCGCCT
GGTCGGGCCGGACGAGTTCGACGTCGCCGCCGGGCTCATCAGCGTGGACGCGCCGCTCGGCCGGGCGCTGCTCGGCAAGC
GCGAGGGCGACGTGGTGGTGGTGCACCGGCCCGCCGGCATGGTGGAATTGACCGTGGTGGAGGTCTCCTGGTCGGACCCG
GAGGGCGGATGA

Upstream 100 bases:

>100_bases
TCCGCACAGGCCCGCGAGAGTAGCCCGGCCCGGCCGCGCGGCGCCAGCTTCCTGCGGTTGACAGCGGCGCCGCTCCGCGG
GAATCGTTCGTCGCACCACC

Downstream 100 bases:

>100_bases
CGATCGAGACGGATCGGGCGGCGCAGCGCGAGCGCGTCGAGCGCTTCGCCCGCGAGCACCCCGGCAGCGTGATGTACGAC
GGCGCCACCCTGCTCGACGT

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MSRTPRYITPEGFRRLAAEHARIWTELRPRIVAEVEAAAALGDRSENAEYIYGKKKLRELDRRLRFLSEKMDSLSVVEPR
AHPDGRAFFGAWVTVEQEDGQARRVRLVGPDEFDVAAGLISVDAPLGRALLGKREGDVVVVHRPAGMVELTVVEVSWSDP
EGG

Sequences:

>Translated_163_residues
MSRTPRYITPEGFRRLAAEHARIWTELRPRIVAEVEAAAALGDRSENAEYIYGKKKLRELDRRLRFLSEKMDSLSVVEPR
AHPDGRAFFGAWVTVEQEDGQARRVRLVGPDEFDVAAGLISVDAPLGRALLGKREGDVVVVHRPAGMVELTVVEVSWSDP
EGG
>Mature_162_residues
SRTPRYITPEGFRRLAAEHARIWTELRPRIVAEVEAAAALGDRSENAEYIYGKKKLRELDRRLRFLSEKMDSLSVVEPRA
HPDGRAFFGAWVTVEQEDGQARRVRLVGPDEFDVAAGLISVDAPLGRALLGKREGDVVVVHRPAGMVELTVVEVSWSDPE
GG

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=44.3037974683544, Blast_Score=143, Evalue=7e-36,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=36.1842105263158, Blast_Score=91, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18177; Mature: 18046

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTPRYITPEGFRRLAAEHARIWTELRPRIVAEVEAAAALGDRSENAEYIYGKKKLREL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHH
DRRLRFLSEKMDSLSVVEPRAHPDGRAFFGAWVTVEQEDGQARRVRLVGPDEFDVAAGLI
HHHHHHHHHHHHCCEEECCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCHHHHHHEE
SVDAPLGRALLGKREGDVVVVHRPAGMVELTVVEVSWSDPEGG
EECCCCCHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
SRTPRYITPEGFRRLAAEHARIWTELRPRIVAEVEAAAALGDRSENAEYIYGKKKLREL
CCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHH
DRRLRFLSEKMDSLSVVEPRAHPDGRAFFGAWVTVEQEDGQARRVRLVGPDEFDVAAGLI
HHHHHHHHHHHHCCEEECCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCHHHHHHEE
SVDAPLGRALLGKREGDVVVVHRPAGMVELTVVEVSWSDPEGG
EECCCCCHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]