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The map label for this gene is wcaJ [H]

Identifier: 220917927

GI number: 220917927

Start: 3127443

End: 3128351

Strand: Reverse

Name: wcaJ [H]

Synonym: A2cp1_2828

Alternate gene names: 220917927

Gene position: 3128351-3127443 (Counterclockwise)

Preceding gene: 220917928

Following gene: 220917926

Centisome position: 62.2

GC content: 74.81

Gene sequence:

>909_bases
GTGGAGCGCGAGGCGGACGGCCTGTGGGCGGTGGCCGGGTTCGTGGTGCCGGAGGCCAGCCGCGCGCCGGCGGTGGTGCC
GCCGGAGGCGCTGCGGCAGGGGGCGGTGCTGGACGTGGTCCGGCAGGCGCGGGCACAGGTGGTGGTGGTGGCCATGGAGG
ACCGGCGCGAGGGGCTGCCGGTGGACGCGCTGCTGGCGCTGCGCACCCGCGGCCTGCGGGTGGTGGACGACGTGGGCTTC
GCGGAGGCCACCCTGCAGCGCATCCCGCTGGCGCTGGTCCGGCCCAGCGCGCTCATCTTCGACGAGGGCTTCCGGGTCTC
GCGCCTGACCCGCACCGTGAAGCGGGCGCTCGACCTGGGCGTGTCCGCGGTGGGCCTGCTGGTGGGCGCGCCGGTGATGG
CCGCGGTGGCCGCCGCGATCTGGCTCTCCGACGGCCGGCCGGTGCTGTACTCGCAGGAGCGCACCGGCCGCGGCGGCCGC
GCGTACCGGATCTGGAAGTTCCGCACCATGCGCCGCGACGCCGAGAAGCTCGGCGCCGCCTGGGCGACCGACGACGACCC
GCGCGTCCTGCCGGTGGGACGCTTCCTGCGGCGCGCCCGGCTGGACGAGCTCCCGCAGCTCTGGAACGTGCTCGGCGGGC
AGATGAGCCTGGTCGGCCCGCGGCCCGAGCGCGCCGTGTTCCTGTCCGAGCTGAAGGCGCGGTGGCCGCTGTTCGCGCTG
CGCGAGCTGGTGAAGCCCGGCCTGACCGGGTGGGCCCAGCTCCGCTACGGCTACGGCTCGACCATGGAGGAGCAGGCGAA
GAAGCTCGAGTACGACCTGTACTACATCAAGAACACCACCCTGTTCCTCGACCTGGTGTGCCTGCTCGCCACGGCGAAGG
TGGTGCTGCTGGGACGGGGGGCGCGATGA

Upstream 100 bases:

>100_bases
GTCATCGGCGCCCGCGCGCTGATGCCGGCGGTGGTGGGGGCGCCCACCCGCGTGCTGTTCCTGGGGGCCGGGCCCCCGTG
CGCGCGACCTGGCGCGGGCG

Downstream 100 bases:

>100_bases
GGGTGCTCGAGGACACGGTGGACCGCCTCGCCGCGGTGCCGGCGGCGCGGCCGCTGCGCGTGGTGGCGGCGGGCGGCGGC
ACCGGGCTGCCGCGGGTGCT

Product: exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MEREADGLWAVAGFVVPEASRAPAVVPPEALRQGAVLDVVRQARAQVVVVAMEDRREGLPVDALLALRTRGLRVVDDVGF
AEATLQRIPLALVRPSALIFDEGFRVSRLTRTVKRALDLGVSAVGLLVGAPVMAAVAAAIWLSDGRPVLYSQERTGRGGR
AYRIWKFRTMRRDAEKLGAAWATDDDPRVLPVGRFLRRARLDELPQLWNVLGGQMSLVGPRPERAVFLSELKARWPLFAL
RELVKPGLTGWAQLRYGYGSTMEEQAKKLEYDLYYIKNTTLFLDLVCLLATAKVVLLGRGAR

Sequences:

>Translated_302_residues
MEREADGLWAVAGFVVPEASRAPAVVPPEALRQGAVLDVVRQARAQVVVVAMEDRREGLPVDALLALRTRGLRVVDDVGF
AEATLQRIPLALVRPSALIFDEGFRVSRLTRTVKRALDLGVSAVGLLVGAPVMAAVAAAIWLSDGRPVLYSQERTGRGGR
AYRIWKFRTMRRDAEKLGAAWATDDDPRVLPVGRFLRRARLDELPQLWNVLGGQMSLVGPRPERAVFLSELKARWPLFAL
RELVKPGLTGWAQLRYGYGSTMEEQAKKLEYDLYYIKNTTLFLDLVCLLATAKVVLLGRGAR
>Mature_302_residues
MEREADGLWAVAGFVVPEASRAPAVVPPEALRQGAVLDVVRQARAQVVVVAMEDRREGLPVDALLALRTRGLRVVDDVGF
AEATLQRIPLALVRPSALIFDEGFRVSRLTRTVKRALDLGVSAVGLLVGAPVMAAVAAAIWLSDGRPVLYSQERTGRGGR
AYRIWKFRTMRRDAEKLGAAWATDDDPRVLPVGRFLRRARLDELPQLWNVLGGQMSLVGPRPERAVFLSELKARWPLFAL
RELVKPGLTGWAQLRYGYGSTMEEQAKKLEYDLYYIKNTTLFLDLVCLLATAKVVLLGRGAR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=188, Percent_Identity=40.4255319148936, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 33425; Mature: 33425

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEREADGLWAVAGFVVPEASRAPAVVPPEALRQGAVLDVVRQARAQVVVVAMEDRREGLP
CCCCCCCCEEEHHEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCEEEEEECCCCCCCC
VDALLALRTRGLRVVDDVGFAEATLQRIPLALVRPSALIFDEGFRVSRLTRTVKRALDLG
HHHHHHHHHCCCEEECCCCHHHHHHHHCCHHHCCCCEEEECCCCHHHHHHHHHHHHHHCC
VSAVGLLVGAPVMAAVAAAIWLSDGRPVLYSQERTGRGGRAYRIWKFRTMRRDAEKLGAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHCCC
WATDDDPRVLPVGRFLRRARLDELPQLWNVLGGQMSLVGPRPERAVFLSELKARWPLFAL
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHH
RELVKPGLTGWAQLRYGYGSTMEEQAKKLEYDLYYIKNTTLFLDLVCLLATAKVVLLGRG
HHHHCCCCCHHHHEECCCCCCHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHHHHEECCC
AR
CC
>Mature Secondary Structure
MEREADGLWAVAGFVVPEASRAPAVVPPEALRQGAVLDVVRQARAQVVVVAMEDRREGLP
CCCCCCCCEEEHHEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCEEEEEECCCCCCCC
VDALLALRTRGLRVVDDVGFAEATLQRIPLALVRPSALIFDEGFRVSRLTRTVKRALDLG
HHHHHHHHHCCCEEECCCCHHHHHHHHCCHHHCCCCEEEECCCCHHHHHHHHHHHHHHCC
VSAVGLLVGAPVMAAVAAAIWLSDGRPVLYSQERTGRGGRAYRIWKFRTMRRDAEKLGAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHCCC
WATDDDPRVLPVGRFLRRARLDELPQLWNVLGGQMSLVGPRPERAVFLSELKARWPLFAL
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHH
RELVKPGLTGWAQLRYGYGSTMEEQAKKLEYDLYYIKNTTLFLDLVCLLATAKVVLLGRG
HHHHCCCCCHHHHEECCCCCCHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHHHHEECCC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]