Click here to switch to the map view.

The map label for this gene is pilJ [H]

Identifier: 220917871

GI number: 220917871

Start: 3057050

End: 3058693

Strand: Direct

Name: pilJ [H]

Synonym: A2cp1_2772

Alternate gene names: 220917871

Gene position: 3057050-3058693 (Clockwise)

Preceding gene: 220917870

Following gene: 220917872

Centisome position: 60.78

GC content: 73.66

Gene sequence:

>1644_bases
ATGCAGACGCAGATCCGCCGGAGCCGATCGCTCGGCTTCCGCCTCGGGGGCTCCGCCCTCGCGCTGTTCGCCGCGGCCGC
GGTGCTCATCGGCCTGAACCTGTGGATGCTCGCCGGCTTGCACGGCGAGGAGGAGTGGACCGCCAAGCTCGTCGAGGGCG
CGGTGCTGCGGTACCAGGCGCTCGATCTCTCGGAGCGGCTGTTCGACGCGCCGGCCGAGCGCGCCCGCACCCGCGAGGCG
CTGGTGCACACCATGACCGCGCTGGACGAGCGCTACCGCGTGCTGCTCCAGGGCGATCCCGAGGCAGGCACCGAGCCCAT
CCGCGACCCGCGGGTGGTGGCCTCGCTCCGGGCGCGAGAGGGGACGTGGACGAACGAGCTCCGGCCCCTGCTCGCCCGCG
TCATGGCCGCGCCCGGCCGCGCCGAGGCGCAGGCCGACCTCGACCGGTTCCGGGAGATCCACGAGCGCTACCTGACCCAG
CTGCAGGGCGACTCGGCGGTGGCCGCGCAGGTGACCGCCGGCCGCGTGCGCCAGTTCCTGGTGGTGCAGGTGCTGTTCGC
GATCCTGGTGATCGCCGTCCTCGCGGCGGTGCTGACGGTCACGCGCGACACCACGCGCCGCATCGGCCGGCTCGCCGCCG
CAGCCGACCGGCTCGCCACGGGCGACCTCGAGGCCCGCGCCGCGGTGCCCGGCCAGGACGAGGTCGCCGCGCTCGGGCGC
TCGTTCGACGCCATGACCGAGAAGGTCCGCAGCGGCCTCACCAAGGAGCAGTCGATGCGGCGCCAGGTGGAGGAACTCCT
CGCGGCGGTGCGGGACACCGCCACGCGGCTCGCCACCGGCACGAACCAGATCGTCACCTCCACCAACCAGCAGGCCACCG
GGGCGCAGGAGCAGGCGGGCGCGGTCACCGAGACGGTCGCGGTGGTCGAGGAGCTGGCACAGATGTCGTCGCAGGCGGCG
CAGCGCGCGCAGGCGGCGGCCGAGACGGCGCGGCGCTCCGACGAGATCGGGCAGGACGGCCGCCGCGCGGTGGAGCGCGC
GGTCGGCTCGATGACCGCGGCGAAGGAGCAGGCCGACTCGGTGGCCGAGAGCATCGTCCGGCTGGCCGAGCAGACGCAGG
CGGTGGGCGACATCGTCAACCTGGTGAACGAGATCTCCGACCAGACCAACCTGCTCGCGCTCAACGCGGCCATCGAGGCG
TCGCGCGCCGGCGAGCAGGGCAAGGGCTTCTCGGTGGTCGCGGCCGAGGTGAAGTCGCTCTCGGAGCAGGCCAAGAAGGC
CACCGTGGAGGTGCGGCGCATCCTGGGCGAGATCCAGAAGATGGCGAGCCGCTCGGTGGTCTCGACCGAGCAGTACTCGA
AGGGGATGGCCCAGGCGACCGAAGCGGCCCGCAGCGCCGGCGAGACCATCCACTCGCTCGCCGGCGCCATCGCCGAGCTG
GCCGAGACCTCGAGCCACATGTCCGCCACCGCCACGCAGCAGGCCCAGGGGCTCGGGCAGATCAACCAGGCCATGCGCGA
CGTGAAGCAGACCACCGACCAGACCGTCCAGGCCATCCGCCAGACCGAGCAGGCGGTCCAGGACCTCTCCACGCTCGCGG
TGCGGCTCACGCAGCTGCTCTCCGAGCCGGACCGGGCCGCCTAG

Upstream 100 bases:

>100_bases
GCGTCACCGCCGCCGGCCTGGCGCTGCTCGACGGCCCGGCCCTGCTCCGTGACCCACGGCTCGTCGTGGACGCCCCTTCC
CTCCCGACCGAGGTGACCTG

Downstream 100 bases:

>100_bases
CGCCGCGCCCGCCATGGACGCCGATCGCCTCCAGGCGCTGCTGCGGGCCACGTTCCTCGGCGAGCTCGAGGACCACGTGC
GCAGCCTGACCGCCGACCTG

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MQTQIRRSRSLGFRLGGSALALFAAAAVLIGLNLWMLAGLHGEEEWTAKLVEGAVLRYQALDLSERLFDAPAERARTREA
LVHTMTALDERYRVLLQGDPEAGTEPIRDPRVVASLRAREGTWTNELRPLLARVMAAPGRAEAQADLDRFREIHERYLTQ
LQGDSAVAAQVTAGRVRQFLVVQVLFAILVIAVLAAVLTVTRDTTRRIGRLAAAADRLATGDLEARAAVPGQDEVAALGR
SFDAMTEKVRSGLTKEQSMRRQVEELLAAVRDTATRLATGTNQIVTSTNQQATGAQEQAGAVTETVAVVEELAQMSSQAA
QRAQAAAETARRSDEIGQDGRRAVERAVGSMTAAKEQADSVAESIVRLAEQTQAVGDIVNLVNEISDQTNLLALNAAIEA
SRAGEQGKGFSVVAAEVKSLSEQAKKATVEVRRILGEIQKMASRSVVSTEQYSKGMAQATEAARSAGETIHSLAGAIAEL
AETSSHMSATATQQAQGLGQINQAMRDVKQTTDQTVQAIRQTEQAVQDLSTLAVRLTQLLSEPDRAA

Sequences:

>Translated_547_residues
MQTQIRRSRSLGFRLGGSALALFAAAAVLIGLNLWMLAGLHGEEEWTAKLVEGAVLRYQALDLSERLFDAPAERARTREA
LVHTMTALDERYRVLLQGDPEAGTEPIRDPRVVASLRAREGTWTNELRPLLARVMAAPGRAEAQADLDRFREIHERYLTQ
LQGDSAVAAQVTAGRVRQFLVVQVLFAILVIAVLAAVLTVTRDTTRRIGRLAAAADRLATGDLEARAAVPGQDEVAALGR
SFDAMTEKVRSGLTKEQSMRRQVEELLAAVRDTATRLATGTNQIVTSTNQQATGAQEQAGAVTETVAVVEELAQMSSQAA
QRAQAAAETARRSDEIGQDGRRAVERAVGSMTAAKEQADSVAESIVRLAEQTQAVGDIVNLVNEISDQTNLLALNAAIEA
SRAGEQGKGFSVVAAEVKSLSEQAKKATVEVRRILGEIQKMASRSVVSTEQYSKGMAQATEAARSAGETIHSLAGAIAEL
AETSSHMSATATQQAQGLGQINQAMRDVKQTTDQTVQAIRQTEQAVQDLSTLAVRLTQLLSEPDRAA
>Mature_547_residues
MQTQIRRSRSLGFRLGGSALALFAAAAVLIGLNLWMLAGLHGEEEWTAKLVEGAVLRYQALDLSERLFDAPAERARTREA
LVHTMTALDERYRVLLQGDPEAGTEPIRDPRVVASLRAREGTWTNELRPLLARVMAAPGRAEAQADLDRFREIHERYLTQ
LQGDSAVAAQVTAGRVRQFLVVQVLFAILVIAVLAAVLTVTRDTTRRIGRLAAAADRLATGDLEARAAVPGQDEVAALGR
SFDAMTEKVRSGLTKEQSMRRQVEELLAAVRDTATRLATGTNQIVTSTNQQATGAQEQAGAVTETVAVVEELAQMSSQAA
QRAQAAAETARRSDEIGQDGRRAVERAVGSMTAAKEQADSVAESIVRLAEQTQAVGDIVNLVNEISDQTNLLALNAAIEA
SRAGEQGKGFSVVAAEVKSLSEQAKKATVEVRRILGEIQKMASRSVVSTEQYSKGMAQATEAARSAGETIHSLAGAIAEL
AETSSHMSATATQQAQGLGQINQAMRDVKQTTDQTVQAIRQTEQAVQDLSTLAVRLTQLLSEPDRAA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=353, Percent_Identity=25.7790368271955, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI2367378, Length=418, Percent_Identity=25.5980861244019, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788195, Length=335, Percent_Identity=26.865671641791, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788194, Length=365, Percent_Identity=25.7534246575342, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789453, Length=341, Percent_Identity=26.099706744868, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 58660; Mature: 58660

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQIRRSRSLGFRLGGSALALFAAAAVLIGLNLWMLAGLHGEEEWTAKLVEGAVLRYQA
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LDLSERLFDAPAERARTREALVHTMTALDERYRVLLQGDPEAGTEPIRDPRVVASLRARE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
GTWTNELRPLLARVMAAPGRAEAQADLDRFREIHERYLTQLQGDSAVAAQVTAGRVRQFL
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VVQVLFAILVIAVLAAVLTVTRDTTRRIGRLAAAADRLATGDLEARAAVPGQDEVAALGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SFDAMTEKVRSGLTKEQSMRRQVEELLAAVRDTATRLATGTNQIVTSTNQQATGAQEQAG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHCCCHHHHH
AVTETVAVVEELAQMSSQAAQRAQAAAETARRSDEIGQDGRRAVERAVGSMTAAKEQADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VAESIVRLAEQTQAVGDIVNLVNEISDQTNLLALNAAIEASRAGEQGKGFSVVAAEVKSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHH
SEQAKKATVEVRRILGEIQKMASRSVVSTEQYSKGMAQATEAARSAGETIHSLAGAIAEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AETSSHMSATATQQAQGLGQINQAMRDVKQTTDQTVQAIRQTEQAVQDLSTLAVRLTQLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEPDRAA
CCCCCCC
>Mature Secondary Structure
MQTQIRRSRSLGFRLGGSALALFAAAAVLIGLNLWMLAGLHGEEEWTAKLVEGAVLRYQA
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LDLSERLFDAPAERARTREALVHTMTALDERYRVLLQGDPEAGTEPIRDPRVVASLRARE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
GTWTNELRPLLARVMAAPGRAEAQADLDRFREIHERYLTQLQGDSAVAAQVTAGRVRQFL
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VVQVLFAILVIAVLAAVLTVTRDTTRRIGRLAAAADRLATGDLEARAAVPGQDEVAALGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SFDAMTEKVRSGLTKEQSMRRQVEELLAAVRDTATRLATGTNQIVTSTNQQATGAQEQAG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHCCCHHHHH
AVTETVAVVEELAQMSSQAAQRAQAAAETARRSDEIGQDGRRAVERAVGSMTAAKEQADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VAESIVRLAEQTQAVGDIVNLVNEISDQTNLLALNAAIEASRAGEQGKGFSVVAAEVKSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHH
SEQAKKATVEVRRILGEIQKMASRSVVSTEQYSKGMAQATEAARSAGETIHSLAGAIAEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AETSSHMSATATQQAQGLGQINQAMRDVKQTTDQTVQAIRQTEQAVQDLSTLAVRLTQLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEPDRAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]