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The map label for this gene is ispDF [H]

Identifier: 220917783

GI number: 220917783

Start: 2962181

End: 2963341

Strand: Reverse

Name: ispDF [H]

Synonym: A2cp1_2684

Alternate gene names: 220917783

Gene position: 2963341-2962181 (Counterclockwise)

Preceding gene: 220917784

Following gene: 220917782

Centisome position: 58.92

GC content: 79.5

Gene sequence:

>1161_bases
GTGATCCGCGGCGAGCGCGTGATCGGGATCCTGGCGGCGGGCGGCTCGGGGCAGCGGGCCGGCGTCGCGAAGCAGTGGCT
ATCCCTGGGCGGCGAGACGGTGCTGCGCCGCTCGGCGCGGGTGCTCGCGGCCTGCGACGCGGTGGACGGGCTGGTGGTGG
TGGTCCCGCCCGGCGACGAGGCGCGCGGCGAGGCGGAGGTGGCCGGGCTGGGGAAGCCGGTGCGGGCCGTGGCCGGCGGC
CCGGCGCGGGCCGACTCGGTGCGCAACGGGCTCGCCGCCGCCGACGGCGCGGTGGTGCTCGTGCACGACGCCGCCCGGCC
GTTCGCGAGCGCGGGGCTGGCGGCGCGGGTGGCCGATGCCGCCGCGCGCGACGGCGCGGCGCTCGCGGCCTTGCCGGCGA
CCGACACCGTGAAGCGCGCCGAGGCCGGCGCCGCGGTGCCGCGCGTCCTCGAGACGCTGGACCGCCGCACGGTGTGGCTG
GCGCAGACGCCGCAGGGCTTCCGCCGCGCGGTGCTCGAGCAGGCCTACGCCGCGGCCGGGCCGTCCGCCTCCGCCGCCAC
CGACGAGTGCGCGCTGGTGGAGGCGGCCGGGGCGCCGGTCACGCTCGTGCCGGGCGAGGCCGGTAACTTCAAGATCACCG
GGCCGGACGACGTGCGCCGCGCCCGCGCGCTGCTCGAGGCGCCGGTCGCGACCGGCGTGGGCTACGACACCCACCGCTTC
GCGCCGGGCCGGCGGCTCGTGCTCGGCGGCGTGGAGTTCGAGGGCGACGGCCTGCTCGGCCACTCCGACGCCGACGTCTG
CGCGCACGCCATCGGCGACGCCATCCTGGGCGCGGCCGGGCTCGGCGACCTCGGCCGGCACTTTCCCGACACCGACCCGC
GCTGGAAGGGCGTCTCCTCGCTGGCGCTGCTCCGCGAGATCGCGGCGAAGGCGGCCGAGCGCGGCTGGCGGGTGGGGAAC
TGCGACGTGACGCTGGCCGCGAGGCGGCCCAAGATCGCGCCGCGCGCCGAGGAGATGCGCGCGCGGCTGGCCGGCGCGCT
CGGGATCTCCCCGGCGCAGGTCAACGTGAAGGCCACCACCGGCGAGGGCATGGGCTTCGTCGGCCGCGAGGAGGGCGTCG
CCGCGCACGCGATCGCCCTGCTGGTGCGCGCGGCCGGCTGA

Upstream 100 bases:

>100_bases
ACGTCTGGGCCCGGGTGCGGCACGCGTCCGGGTGATAGCAGGGGCGGTGCCAGCCGGCGCCGCGGGACGGGCGGCGCGCG
CGCCGCGAGGAGGCGACGAG

Downstream 100 bases:

>100_bases
GCCGGACCCCGCCGACGAACGGGACACGACAGAGATGAGCATCCAGGTCCACGACACCCTGACCGCGCAGAAGCGCGAGC
TGGTCCCCCTCGAGCCGGGC

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MIRGERVIGILAAGGSGQRAGVAKQWLSLGGETVLRRSARVLAACDAVDGLVVVVPPGDEARGEAEVAGLGKPVRAVAGG
PARADSVRNGLAAADGAVVLVHDAARPFASAGLAARVADAAARDGAALAALPATDTVKRAEAGAAVPRVLETLDRRTVWL
AQTPQGFRRAVLEQAYAAAGPSASAATDECALVEAAGAPVTLVPGEAGNFKITGPDDVRRARALLEAPVATGVGYDTHRF
APGRRLVLGGVEFEGDGLLGHSDADVCAHAIGDAILGAAGLGDLGRHFPDTDPRWKGVSSLALLREIAAKAAERGWRVGN
CDVTLAARRPKIAPRAEEMRARLAGALGISPAQVNVKATTGEGMGFVGREEGVAAHAIALLVRAAG

Sequences:

>Translated_386_residues
MIRGERVIGILAAGGSGQRAGVAKQWLSLGGETVLRRSARVLAACDAVDGLVVVVPPGDEARGEAEVAGLGKPVRAVAGG
PARADSVRNGLAAADGAVVLVHDAARPFASAGLAARVADAAARDGAALAALPATDTVKRAEAGAAVPRVLETLDRRTVWL
AQTPQGFRRAVLEQAYAAAGPSASAATDECALVEAAGAPVTLVPGEAGNFKITGPDDVRRARALLEAPVATGVGYDTHRF
APGRRLVLGGVEFEGDGLLGHSDADVCAHAIGDAILGAAGLGDLGRHFPDTDPRWKGVSSLALLREIAAKAAERGWRVGN
CDVTLAARRPKIAPRAEEMRARLAGALGISPAQVNVKATTGEGMGFVGREEGVAAHAIALLVRAAG
>Mature_386_residues
MIRGERVIGILAAGGSGQRAGVAKQWLSLGGETVLRRSARVLAACDAVDGLVVVVPPGDEARGEAEVAGLGKPVRAVAGG
PARADSVRNGLAAADGAVVLVHDAARPFASAGLAARVADAAARDGAALAALPATDTVKRAEAGAAVPRVLETLDRRTVWL
AQTPQGFRRAVLEQAYAAAGPSASAATDECALVEAAGAPVTLVPGEAGNFKITGPDDVRRARALLEAPVATGVGYDTHRF
APGRRLVLGGVEFEGDGLLGHSDADVCAHAIGDAILGAAGLGDLGRHFPDTDPRWKGVSSLALLREIAAKAAERGWRVGN
CDVTLAARRPKIAPRAEEMRARLAGALGISPAQVNVKATTGEGMGFVGREEGVAAHAIALLVRAAG

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=297, Percent_Identity=25.2525252525253, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=49.0322580645161, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1789104, Length=224, Percent_Identity=38.3928571428571, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 39052; Mature: 39052

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRGERVIGILAAGGSGQRAGVAKQWLSLGGETVLRRSARVLAACDAVDGLVVVVPPGDE
CCCCCEEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
ARGEAEVAGLGKPVRAVAGGPARADSVRNGLAAADGAVVLVHDAARPFASAGLAARVADA
CCCCCHHHCCCCCHHHHCCCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHCCHHHHHHHH
AARDGAALAALPATDTVKRAEAGAAVPRVLETLDRRTVWLAQTPQGFRRAVLEQAYAAAG
HHCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCC
PSASAATDECALVEAAGAPVTLVPGEAGNFKITGPDDVRRARALLEAPVATGVGYDTHRF
CCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCC
APGRRLVLGGVEFEGDGLLGHSDADVCAHAIGDAILGAAGLGDLGRHFPDTDPRWKGVSS
CCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHH
LALLREIAAKAAERGWRVGNCDVTLAARRPKIAPRAEEMRARLAGALGISPAQVNVKATT
HHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEC
GEGMGFVGREEGVAAHAIALLVRAAG
CCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRGERVIGILAAGGSGQRAGVAKQWLSLGGETVLRRSARVLAACDAVDGLVVVVPPGDE
CCCCCEEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
ARGEAEVAGLGKPVRAVAGGPARADSVRNGLAAADGAVVLVHDAARPFASAGLAARVADA
CCCCCHHHCCCCCHHHHCCCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHCCHHHHHHHH
AARDGAALAALPATDTVKRAEAGAAVPRVLETLDRRTVWLAQTPQGFRRAVLEQAYAAAG
HHCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCC
PSASAATDECALVEAAGAPVTLVPGEAGNFKITGPDDVRRARALLEAPVATGVGYDTHRF
CCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCC
APGRRLVLGGVEFEGDGLLGHSDADVCAHAIGDAILGAAGLGDLGRHFPDTDPRWKGVSS
CCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHH
LALLREIAAKAAERGWRVGNCDVTLAARRPKIAPRAEEMRARLAGALGISPAQVNVKATT
HHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEC
GEGMGFVGREEGVAAHAIALLVRAAG
CCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA