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The map label for this gene is cysS

Identifier: 220917782

GI number: 220917782

Start: 2960701

End: 2962146

Strand: Reverse

Name: cysS

Synonym: A2cp1_2683

Alternate gene names: 220917782

Gene position: 2962146-2960701 (Counterclockwise)

Preceding gene: 220917783

Following gene: 220917778

Centisome position: 58.9

GC content: 72.68

Gene sequence:

>1446_bases
ATGAGCATCCAGGTCCACGACACCCTGACCGCGCAGAAGCGCGAGCTGGTCCCCCTCGAGCCGGGCAAGCTCCGGCTCTA
CGTGTGCGGCCCCACCGTCTACGACTACTCGCACCTCGGCCACGCCCGCTGCTACGTGGTCTGGGACGTGGTGGTCCGTC
ACCTGCGCGCGCGCGGCCTCGAGGTGCGCTTCGTCCGCAACTTCACCGACGTGGACGACAAGATCATCCAGCGCGCCAAC
GAGCGCGGCGAGGACCCCATCGCGCTCGCGTCGCGCTTCGCCGACGCGTTCCACGAGGACATGGACGCGCTCGGCAACCT
GCGCCCCGACGTCGAGCCGCGGGTGTCCGGCCACATCCCGGAGATCGTCGCGCTGATCGCCCGGCTGGTGGAGCGAGGCT
TCGCCTACGCGCCCGGGAACGGCGACGTCTACTACGCGGTCCGCAAGTTCCCCGAGTACGGGCGGCTCTCGAAGCGCAAC
CTGGACGACCTCATCGCCGGCGCGCGGGTGGAGCCGGGCGAGGCCAAGCGCGACCCGCTCGACTTCGCGCTGTGGAAGGC
GGCGAAGCCGGGCGAGCCGGCCTGGGAGTCGCCCTGGGGCAAGGGCCGGCCGGGCTGGCACATCGAGTGCTCGGCCATGA
CCCAGAAGCACCTGGGCGCGCCCATCGACCTGCACGCCGGCGGCAAGGACCTGGTCTTCCCGCACCACACCAACGAGATC
GCGCAGTCGGTGGCGGCCACCAGCGACGGCCTGCACGCCGAGGACTTCGCGCGGTACTGGATGCACAACGGCTTCGTCCA
GATCGACGACGAGAAGATGTCGAAGTCGCTCGGCAACTTCTTCACCATCCGCGACGTGCTGGCCCGCTTCGACGGCGAGG
CCCTGCGCTTCTTCCTGCTCGGCACGCACTACCGCCGCGACTTCAACTTCTCCGACCAGGTGCTGGCCGAGGCCGAGCGG
CGCCTCTCCGCGCTCTACGAGACGGTGGAGAAGGCCGAGCGGCTGGGCGCGGGCGCGGCGCCGGCCGCGGAGCCCGCGTT
CGTGGAGCGGGCGCGCGCCGCGCTCGACGACGACTTCAACACCCCGCAGGTGCTGGGCATCGTGGCCGAGGCGTTCACCG
AGGCGAACGCCCTCGCGGATCGCAAGGGCAAGAAGTCGTCCGAGGAGAAGGCGCGGCTGGCCGCCTTCGCCCGCGGCGCG
CGCGCGGTGGGCGCGGTGCTCGGGATCCTGGACCGCCCGCCGGCGCAGGCGCTCACGGCCATCCGCGACCGGGCGGCGGC
GCGGCGGGGCATCGACGGCGGCGAGGTGGAGCGGTCCATCGCGGAGCGCGCGGCGGCGCGGGCGGCGAAGGACTTCGCGC
GCTCCGACGCCATCCGCGACGCGCTCCTGGCCCGCGGCGTCGTCCTGATGGACGGGCCGCAGGGCACGACCTGGAAGGTG
GAGTAG

Upstream 100 bases:

>100_bases
TTCGTCGGCCGCGAGGAGGGCGTCGCCGCGCACGCGATCGCCCTGCTGGTGCGCGCGGCCGGCTGAGCCGGACCCCGCCG
ACGAACGGGACACGACAGAG

Downstream 100 bases:

>100_bases
GGGCAGGCGCGCGGCCGCGCGCCTCGCCGGCTCATTCCTCGAGCGGCGGCTGCGGGCCGGGGACGATGCCGGCGATGTCC
GGATCCTCGCCGGGGGCGAG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSIQVHDTLTAQKRELVPLEPGKLRLYVCGPTVYDYSHLGHARCYVVWDVVVRHLRARGLEVRFVRNFTDVDDKIIQRAN
ERGEDPIALASRFADAFHEDMDALGNLRPDVEPRVSGHIPEIVALIARLVERGFAYAPGNGDVYYAVRKFPEYGRLSKRN
LDDLIAGARVEPGEAKRDPLDFALWKAAKPGEPAWESPWGKGRPGWHIECSAMTQKHLGAPIDLHAGGKDLVFPHHTNEI
AQSVAATSDGLHAEDFARYWMHNGFVQIDDEKMSKSLGNFFTIRDVLARFDGEALRFFLLGTHYRRDFNFSDQVLAEAER
RLSALYETVEKAERLGAGAAPAAEPAFVERARAALDDDFNTPQVLGIVAEAFTEANALADRKGKKSSEEKARLAAFARGA
RAVGAVLGILDRPPAQALTAIRDRAAARRGIDGGEVERSIAERAAARAAKDFARSDAIRDALLARGVVLMDGPQGTTWKV
E

Sequences:

>Translated_481_residues
MSIQVHDTLTAQKRELVPLEPGKLRLYVCGPTVYDYSHLGHARCYVVWDVVVRHLRARGLEVRFVRNFTDVDDKIIQRAN
ERGEDPIALASRFADAFHEDMDALGNLRPDVEPRVSGHIPEIVALIARLVERGFAYAPGNGDVYYAVRKFPEYGRLSKRN
LDDLIAGARVEPGEAKRDPLDFALWKAAKPGEPAWESPWGKGRPGWHIECSAMTQKHLGAPIDLHAGGKDLVFPHHTNEI
AQSVAATSDGLHAEDFARYWMHNGFVQIDDEKMSKSLGNFFTIRDVLARFDGEALRFFLLGTHYRRDFNFSDQVLAEAER
RLSALYETVEKAERLGAGAAPAAEPAFVERARAALDDDFNTPQVLGIVAEAFTEANALADRKGKKSSEEKARLAAFARGA
RAVGAVLGILDRPPAQALTAIRDRAAARRGIDGGEVERSIAERAAARAAKDFARSDAIRDALLARGVVLMDGPQGTTWKV
E
>Mature_480_residues
SIQVHDTLTAQKRELVPLEPGKLRLYVCGPTVYDYSHLGHARCYVVWDVVVRHLRARGLEVRFVRNFTDVDDKIIQRANE
RGEDPIALASRFADAFHEDMDALGNLRPDVEPRVSGHIPEIVALIARLVERGFAYAPGNGDVYYAVRKFPEYGRLSKRNL
DDLIAGARVEPGEAKRDPLDFALWKAAKPGEPAWESPWGKGRPGWHIECSAMTQKHLGAPIDLHAGGKDLVFPHHTNEIA
QSVAATSDGLHAEDFARYWMHNGFVQIDDEKMSKSLGNFFTIRDVLARFDGEALRFFLLGTHYRRDFNFSDQVLAEAERR
LSALYETVEKAERLGAGAAPAAEPAFVERARAALDDDFNTPQVLGIVAEAFTEANALADRKGKKSSEEKARLAAFARGAR
AVGAVLGILDRPPAQALTAIRDRAAARRGIDGGEVERSIAERAAARAAKDFARSDAIRDALLARGVVLMDGPQGTTWKVE

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=379, Percent_Identity=41.688654353562, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI303304987, Length=253, Percent_Identity=43.0830039525692, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI10835051, Length=253, Percent_Identity=43.0830039525692, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI62240992, Length=253, Percent_Identity=43.0830039525692, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI21269877, Length=253, Percent_Identity=43.0830039525692, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI1786737, Length=477, Percent_Identity=48.0083857442348, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17509795, Length=248, Percent_Identity=39.1129032258064, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17509797, Length=248, Percent_Identity=39.1129032258064, Blast_Score=177, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6324082, Length=400, Percent_Identity=31.75, Blast_Score=206, Evalue=7e-54,
Organism=Drosophila melanogaster, GI19922218, Length=503, Percent_Identity=33.3996023856859, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI19922364, Length=296, Percent_Identity=37.8378378378378, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ANAD2 (B8JDH1)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493086.1
- ProteinModelPortal:   B8JDH1
- GeneID:   7299684
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2683
- HOGENOM:   HBG327651
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53137; Mature: 53005

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQVHDTLTAQKRELVPLEPGKLRLYVCGPTVYDYSHLGHARCYVVWDVVVRHLRARGL
CEEEECCCHHHHHCCCCCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EVRFVRNFTDVDDKIIQRANERGEDPIALASRFADAFHEDMDALGNLRPDVEPRVSGHIP
EEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
EIVALIARLVERGFAYAPGNGDVYYAVRKFPEYGRLSKRNLDDLIAGARVEPGEAKRDPL
HHHHHHHHHHHCCCEECCCCCCEEEHEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
DFALWKAAKPGEPAWESPWGKGRPGWHIECSAMTQKHLGAPIDLHAGGKDLVFPHHTNEI
HHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCEEECCCCCCCCCCCCHHHH
AQSVAATSDGLHAEDFARYWMHNGFVQIDDEKMSKSLGNFFTIRDVLARFDGEALRFFLL
HHHHHHCCCCCCHHHHHHHHHHCCCEEECHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHH
GTHYRRDFNFSDQVLAEAERRLSALYETVEKAERLGAGAAPAAEPAFVERARAALDDDFN
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC
TPQVLGIVAEAFTEANALADRKGKKSSEEKARLAAFARGARAVGAVLGILDRPPAQALTA
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IRDRAAARRGIDGGEVERSIAERAAARAAKDFARSDAIRDALLARGVVLMDGPQGTTWKV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEC
E
C
>Mature Secondary Structure 
SIQVHDTLTAQKRELVPLEPGKLRLYVCGPTVYDYSHLGHARCYVVWDVVVRHLRARGL
EEEECCCHHHHHCCCCCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EVRFVRNFTDVDDKIIQRANERGEDPIALASRFADAFHEDMDALGNLRPDVEPRVSGHIP
EEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
EIVALIARLVERGFAYAPGNGDVYYAVRKFPEYGRLSKRNLDDLIAGARVEPGEAKRDPL
HHHHHHHHHHHCCCEECCCCCCEEEHEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
DFALWKAAKPGEPAWESPWGKGRPGWHIECSAMTQKHLGAPIDLHAGGKDLVFPHHTNEI
HHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCEEECCCCCCCCCCCCHHHH
AQSVAATSDGLHAEDFARYWMHNGFVQIDDEKMSKSLGNFFTIRDVLARFDGEALRFFLL
HHHHHHCCCCCCHHHHHHHHHHCCCEEECHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHH
GTHYRRDFNFSDQVLAEAERRLSALYETVEKAERLGAGAAPAAEPAFVERARAALDDDFN
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC
TPQVLGIVAEAFTEANALADRKGKKSSEEKARLAAFARGARAVGAVLGILDRPPAQALTA
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IRDRAAARRGIDGGEVERSIAERAAARAAKDFARSDAIRDALLARGVVLMDGPQGTTWKV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA