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The map label for this gene is glyS

Identifier: 220917749

GI number: 220917749

Start: 2927589

End: 2929694

Strand: Reverse

Name: glyS

Synonym: A2cp1_2650

Alternate gene names: 220917749

Gene position: 2929694-2927589 (Counterclockwise)

Preceding gene: 220917750

Following gene: 220917748

Centisome position: 58.25

GC content: 74.79

Gene sequence:

>2106_bases
ATGGCTGACCTTCTGTTCGAGATCGGTGCGGAGGAGATCCCGGCGGGCTTCGTGCCGGGCGCGCTCAGGCAGCTCGAGGA
CGACCTGTCGAAGGCGCTCGCCGACGCGCGGCTGGCGCACGGTGAGGTGAGGTCCGTCGGCACGCCGCGCCGCCTGGCGG
TCTGGGCGCGCGACGTGGCGCCGAAGCAGACCGACGCGCGCACCGAGGCGTTCGGGCCCCCGGTGGCGCAGGCCTACGAC
GCCGAGGGCAAGCCTACCCCGGCCGCGACCGGCTTCGCGCGCAGCCAGGGCGTGGAGGTCTCGGCGCTGGTCCGCGCCCA
GACGCCCAAGGGCGAGCGCGTCGCGGTCACGAAGGTGGAGAAGGGCCGCAGGGCGGAGCAGGTCCTGCCCGCGCTGCTGG
AGCGGCTCGTCGGCGGCCTGCGCTTCCGCAAGGCGATGCGCTCGCGCTTCGACGAGGCGACCTTCGCGCGCCCGGTGCGC
TGGATGGTGGCGCTCCTCGGCGGGCGCCCGCTCAAGGTGCGCCACGGCGAGGTCACCTCGGGCAAGGTCACCTACGGCCA
CCGCTTCCTCGCGCCGAAGGCGATCGCGCTGAAGGGCACGCCGGACGACTACCTGGCGAAGCTGCGCCGGGCGCACGTCC
TCGCCGACCCGGTGGAGCGCCGCGCCGCGCTGCTCGCCGAGCTGGCCCGCGCCGGCAAGGAGGCCGCGGGGAAGGTCCGC
GACGATCCCGCGCTGGTCGAGCAGGTGCTGTACCTGGTGGAGGAGCCGACCGCGGTGGTCGGCGAGTTCGAGAAGTCGAA
CCTCGAGCTGCCGCCGGAGGTGGTGATCTCCGAGATGCGCAACCACCAGCGCTACTTCGCGGTGGTGGACGGCAAGGGGC
GGCTCAAGAACCGCTTCGTGGCCGTCTCCGCCACGCGGGTGAAGGATCCCGCGGTGGCGCGGCACGGCTACGAGCGCGTG
CTCCGCGCGCGCCTCGCCGACGCGCGCTTCTTCTTCGAGGAGGACCGCAAGCGCAGGCTGCACGAGCGCATCGAGGACCT
GGGTCGCCGCACGTTCCAGGCGAAGCTGGGGAGCGAGCTGGATCGCGCCCAGCGCATCGGCGCGGTGGCGAGCGCGCTGG
CCCGCGCGCTCGGGAAGGACGCGCTGGTCGCCGACCTGCTCGAGGCCTCGCGGCTCGCCAAGGTGGACCTCAACACCGGC
ATGGTGGGCGAGTTCCCCGAGCTGCAGGGCACCATGGGCGCGCACTACGCGCGGCTGGAGGGGCTGAAGCCGGAGATCGC
CGACGCGATCGAGGACCACTACAAGCCCATCGGCGCGGCCGAGGAGCTGCCGCGCAGCGACCTGGGCGCGCTGGTGGCGG
TGGCCGACCGCCTCCACTCGCTCGTGGGCATCATCGGCGTGGGCGAGAAGGCGACCGGCGCCGCCGATCCGTTCGGCCTG
CGCCGCTCCGCCATCGGCATCCTGCGCATCGTCATCGCCCGCGGGTACCACCTGTCGCTCGCCGCGGCGGTGGAGCAGAC
GCTCGACGCGCTCTCCGGGGTGAAGCTCGCCGCCGGCCGCGCGGTGGTGGCCGAGCAGGTGCTGGACTTCCTGCGCGGGC
GCGTGCGCGCCGCCTGGACCGAGCGGTTCGACGCCGACCTGGTGGAGGCGGTGCTCGCGGCCGGCTCCGACGACGTGGTG
GACGCCCGCCGGCGCCTGGAGGCGCTCGCCGACGCCAAGGCGCGACCCGACTTCGGCTCGCTCGCCGTCGCGTTCAAGCG
CGTCGCGAACATCCAGGAGAAGGCGGGCGGCTCCGGCGCGGCCGCGGTGGATCCGGCGCTGCTGCGCGACGCGGCGGAGA
AGGACCTGCTGGCGGCGCTCGAGAAGGTGGAGCAGGAGGTGGTGGCGCGCCGCGCGGCCCGCGACTACCCGGCCGTGCTC
CGCACGGTGGCGACGCTGGAGCCGGCGGTGGCGCGCTTCTTCGACGGCGTGCTGGTGATGGCCGAGGACCCCGCGCTCCG
CGCCAACCGGCTCGGCCTGATGCGGCGGGTGGCGGCGCTGTTCTCCGACCTCGCCGACTTCCGCAAGATCCAGGCCGAGG
CGCCCGCCCAGGCCAGGGCCGGGTAG

Upstream 100 bases:

>100_bases
TGCGCGGGCGGCCCGCGAGGCGCGCGAGTCCAGGTGACGACCCCTCACCCCCGGGCCTCCCTCGCCGCGCGCGGCGGGGG
AGCGGGGACGGAACGAGATC

Downstream 100 bases:

>100_bases
GTGGAGCGCTTGACCCTCTCTCGGACGCCGGATTACGGTTCGCACCCCGTGCACCACGTCGCTCGACGCAGCGCCCCGAT
CCTCCTGATGTGTCTCGCGG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MADLLFEIGAEEIPAGFVPGALRQLEDDLSKALADARLAHGEVRSVGTPRRLAVWARDVAPKQTDARTEAFGPPVAQAYD
AEGKPTPAATGFARSQGVEVSALVRAQTPKGERVAVTKVEKGRRAEQVLPALLERLVGGLRFRKAMRSRFDEATFARPVR
WMVALLGGRPLKVRHGEVTSGKVTYGHRFLAPKAIALKGTPDDYLAKLRRAHVLADPVERRAALLAELARAGKEAAGKVR
DDPALVEQVLYLVEEPTAVVGEFEKSNLELPPEVVISEMRNHQRYFAVVDGKGRLKNRFVAVSATRVKDPAVARHGYERV
LRARLADARFFFEEDRKRRLHERIEDLGRRTFQAKLGSELDRAQRIGAVASALARALGKDALVADLLEASRLAKVDLNTG
MVGEFPELQGTMGAHYARLEGLKPEIADAIEDHYKPIGAAEELPRSDLGALVAVADRLHSLVGIIGVGEKATGAADPFGL
RRSAIGILRIVIARGYHLSLAAAVEQTLDALSGVKLAAGRAVVAEQVLDFLRGRVRAAWTERFDADLVEAVLAAGSDDVV
DARRRLEALADAKARPDFGSLAVAFKRVANIQEKAGGSGAAAVDPALLRDAAEKDLLAALEKVEQEVVARRAARDYPAVL
RTVATLEPAVARFFDGVLVMAEDPALRANRLGLMRRVAALFSDLADFRKIQAEAPAQARAG

Sequences:

>Translated_701_residues
MADLLFEIGAEEIPAGFVPGALRQLEDDLSKALADARLAHGEVRSVGTPRRLAVWARDVAPKQTDARTEAFGPPVAQAYD
AEGKPTPAATGFARSQGVEVSALVRAQTPKGERVAVTKVEKGRRAEQVLPALLERLVGGLRFRKAMRSRFDEATFARPVR
WMVALLGGRPLKVRHGEVTSGKVTYGHRFLAPKAIALKGTPDDYLAKLRRAHVLADPVERRAALLAELARAGKEAAGKVR
DDPALVEQVLYLVEEPTAVVGEFEKSNLELPPEVVISEMRNHQRYFAVVDGKGRLKNRFVAVSATRVKDPAVARHGYERV
LRARLADARFFFEEDRKRRLHERIEDLGRRTFQAKLGSELDRAQRIGAVASALARALGKDALVADLLEASRLAKVDLNTG
MVGEFPELQGTMGAHYARLEGLKPEIADAIEDHYKPIGAAEELPRSDLGALVAVADRLHSLVGIIGVGEKATGAADPFGL
RRSAIGILRIVIARGYHLSLAAAVEQTLDALSGVKLAAGRAVVAEQVLDFLRGRVRAAWTERFDADLVEAVLAAGSDDVV
DARRRLEALADAKARPDFGSLAVAFKRVANIQEKAGGSGAAAVDPALLRDAAEKDLLAALEKVEQEVVARRAARDYPAVL
RTVATLEPAVARFFDGVLVMAEDPALRANRLGLMRRVAALFSDLADFRKIQAEAPAQARAG
>Mature_700_residues
ADLLFEIGAEEIPAGFVPGALRQLEDDLSKALADARLAHGEVRSVGTPRRLAVWARDVAPKQTDARTEAFGPPVAQAYDA
EGKPTPAATGFARSQGVEVSALVRAQTPKGERVAVTKVEKGRRAEQVLPALLERLVGGLRFRKAMRSRFDEATFARPVRW
MVALLGGRPLKVRHGEVTSGKVTYGHRFLAPKAIALKGTPDDYLAKLRRAHVLADPVERRAALLAELARAGKEAAGKVRD
DPALVEQVLYLVEEPTAVVGEFEKSNLELPPEVVISEMRNHQRYFAVVDGKGRLKNRFVAVSATRVKDPAVARHGYERVL
RARLADARFFFEEDRKRRLHERIEDLGRRTFQAKLGSELDRAQRIGAVASALARALGKDALVADLLEASRLAKVDLNTGM
VGEFPELQGTMGAHYARLEGLKPEIADAIEDHYKPIGAAEELPRSDLGALVAVADRLHSLVGIIGVGEKATGAADPFGLR
RSAIGILRIVIARGYHLSLAAAVEQTLDALSGVKLAAGRAVVAEQVLDFLRGRVRAAWTERFDADLVEAVLAAGSDDVVD
ARRRLEALADAKARPDFGSLAVAFKRVANIQEKAGGSGAAAVDPALLRDAAEKDLLAALEKVEQEVVARRAARDYPAVLR
TVATLEPAVARFFDGVLVMAEDPALRANRLGLMRRVAALFSDLADFRKIQAEAPAQARAG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=694, Percent_Identity=35.7348703170029, Blast_Score=371, Evalue=1e-103,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_ANAD2 (B8JDD8)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493053.1
- ProteinModelPortal:   B8JDD8
- GeneID:   7299651
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2650
- HOGENOM:   HBG586599
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75778; Mature: 75647

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLLFEIGAEEIPAGFVPGALRQLEDDLSKALADARLAHGEVRSVGTPRRLAVWARDVA
CCCHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHCC
PKQTDARTEAFGPPVAQAYDAEGKPTPAATGFARSQGVEVSALVRAQTPKGERVAVTKVE
CCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCCEEEEEECH
KGRRAEQVLPALLERLVGGLRFRKAMRSRFDEATFARPVRWMVALLGGRPLKVRHGEVTS
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
GKVTYGHRFLAPKAIALKGTPDDYLAKLRRAHVLADPVERRAALLAELARAGKEAAGKVR
CEEECCCHHHCCCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCHHHCCCCC
DDPALVEQVLYLVEEPTAVVGEFEKSNLELPPEVVISEMRNHQRYFAVVDGKGRLKNRFV
CCHHHHHHHHHHHCCCHHHHHCHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEE
AVSATRVKDPAVARHGYERVLRARLADARFFFEEDRKRRLHERIEDLGRRTFQAKLGSEL
EEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRAQRIGAVASALARALGKDALVADLLEASRLAKVDLNTGMVGEFPELQGTMGAHYARLE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCCCHHHCHHHHHHHHHC
GLKPEIADAIEDHYKPIGAAEELPRSDLGALVAVADRLHSLVGIIGVGEKATGAADPFGL
CCCHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RRSAIGILRIVIARGYHLSLAAAVEQTLDALSGVKLAAGRAVVAEQVLDFLRGRVRAAWT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
ERFDADLVEAVLAAGSDDVVDARRRLEALADAKARPDFGSLAVAFKRVANIQEKAGGSGA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AAVDPALLRDAAEKDLLAALEKVEQEVVARRAARDYPAVLRTVATLEPAVARFFDGVLVM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEE
AEDPALRANRLGLMRRVAALFSDLADFRKIQAEAPAQARAG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADLLFEIGAEEIPAGFVPGALRQLEDDLSKALADARLAHGEVRSVGTPRRLAVWARDVA
CCHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHCC
PKQTDARTEAFGPPVAQAYDAEGKPTPAATGFARSQGVEVSALVRAQTPKGERVAVTKVE
CCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCCEEEEEECH
KGRRAEQVLPALLERLVGGLRFRKAMRSRFDEATFARPVRWMVALLGGRPLKVRHGEVTS
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
GKVTYGHRFLAPKAIALKGTPDDYLAKLRRAHVLADPVERRAALLAELARAGKEAAGKVR
CEEECCCHHHCCCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCHHHCCCCC
DDPALVEQVLYLVEEPTAVVGEFEKSNLELPPEVVISEMRNHQRYFAVVDGKGRLKNRFV
CCHHHHHHHHHHHCCCHHHHHCHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEE
AVSATRVKDPAVARHGYERVLRARLADARFFFEEDRKRRLHERIEDLGRRTFQAKLGSEL
EEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRAQRIGAVASALARALGKDALVADLLEASRLAKVDLNTGMVGEFPELQGTMGAHYARLE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCCCHHHCHHHHHHHHHC
GLKPEIADAIEDHYKPIGAAEELPRSDLGALVAVADRLHSLVGIIGVGEKATGAADPFGL
CCCHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RRSAIGILRIVIARGYHLSLAAAVEQTLDALSGVKLAAGRAVVAEQVLDFLRGRVRAAWT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
ERFDADLVEAVLAAGSDDVVDARRRLEALADAKARPDFGSLAVAFKRVANIQEKAGGSGA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AAVDPALLRDAAEKDLLAALEKVEQEVVARRAARDYPAVLRTVATLEPAVARFFDGVLVM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEE
AEDPALRANRLGLMRRVAALFSDLADFRKIQAEAPAQARAG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA