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The map label for this gene is tilS [H]

Identifier: 220917561

GI number: 220917561

Start: 2719106

End: 2720458

Strand: Reverse

Name: tilS [H]

Synonym: A2cp1_2461

Alternate gene names: 220917561

Gene position: 2720458-2719106 (Counterclockwise)

Preceding gene: 220917566

Following gene: 220917560

Centisome position: 54.09

GC content: 81.6

Gene sequence:

>1353_bases
ATGCGACGCCCTGACCTCCACCCGTACCCCGCCGCCGTGCTCGAGACCGCCCGCCGCCGGCGGCTGTTCGGCCCCGCGGA
CCGCGTGCTCGTGGCGCTCTCGGGCGGCGCCGACTCGACGGCCCTGCTCGCCGCCCTCGCGGCGCTGCGCGACGCCGGCG
CGCTCGCGGAGGTGCGGGCCCTGTACCTGGACCACGGGCTGCGCGCGGGCAGCGAGCCCGAGGCGGCGTCGGCCCGCGCC
GCGTGCGCGCGGCTCGGCGTGCCGCTCGAGGAGCGGCGCCTCGCGGTGGGGGCCGGCAACGTGCAGGCCGAGGCCCGCCG
GGTGCGCTACGCGGCGCTGCGCGAGGCGGCGGCGCGCGCCGGCGCGTCGCGGATCGCCACCGGGCACACGCTGGGCGACC
AGGCCGAGACCGTGCTGCTGCGCCTGCTCCGCGGCGCCGGCGCGCGCGGGCTCGCCGCGATCCCGCCGCGGCGCGGCCCG
GTGATCCGGCCCCTCATCGATCGCACGCGCGAGGAGGGGAACGCCTACCTGCGCGCGATGGGGCTGGGCTGGGCCGACGA
CCCCACCAACGCCGTCCCGGGCTACGCGCGGAACCGGCTCCGGCTCGAGCTCTGGCCGCTGCTGCGGGCGGCGGCGCCGG
CGGCGGACCGGGCGCTGGCGCGCGCGGCCGACCTGGCGCGCGAGGACGAGCGCGCGCTCGATCGCCGGGCGCGCGCGGTC
GCGGGGGGCGGCACCGCGGTGGACGTGGCCGCGCTCCGGGGCGAGCCGCTCGCGGTGCGGCGGCGGGTGGTGCGGCGGCT
CTGGCGCGCCGCGACCGGCAGCGGCCGGGCGCTCGAGGCGCAGCACGTGGCGGCGGTGCTGGCGCTCCTGCGCCGCGGGC
GGCCGGGCGAGGTCGCGCTCCCGGGCGGGCGGCGCGCCCGGTGCCGCTACGGGCGCCTCGAGCTCGCCGGCCCCGCGGCG
CCCGTCCCGGTGGTGGCGCCGGTGGAGGTGCCGGGGCCGGGGAGGTATGCGCTCCCGGCACTGGACGCCTGGGTGGAGGT
CGGCGCCGCGCGGCCGGCCGAGGTGCCCTGGCCGCTCACCCTCCGGACCCGCCGCCCGGGGGACCGCTTCCGGCCGGAGG
GCGGGCCGGGCGGGACGAAGCTGAAGCGCTGGCTCATCGACCGGAAGGTGCCGCGCGAGGCGCGGGATCGGCTGGTGCTC
CTCGCCGCACCCGGGGGCGCGGTGCTGGCGGTTCCGGAGCTGGGCGCCGTCGCGGAAGGGCTGGGCCCTTCGGGCGCCGG
GTTGCGCGTGCGGATTTCGACCGCGCCCGGCCCGGACGGCTCGTACTGCAAAGGGGGTGAGGGCCTGTTATAA

Upstream 100 bases:

>100_bases
TCGAACCCGCCGGCCGGCGCTCGCATTCCCGGCGCGGGTCGAGGTCCGGTGCACGCCCGACCCGCGCACCTCCGCGCCGG
GGCCGAGGAGCCCCTCCTGC

Downstream 100 bases:

>100_bases
CGGGCACCTGGCCCGGGCGTCGCCTGGCCGGGCGCCTGGCGGCGCGGGGCGCGGTGCGGAAGAGTCGGACCTCGCCGTCG
GTTCCGGTCGATCGACGCTC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MRRPDLHPYPAAVLETARRRRLFGPADRVLVALSGGADSTALLAALAALRDAGALAEVRALYLDHGLRAGSEPEAASARA
ACARLGVPLEERRLAVGAGNVQAEARRVRYAALREAAARAGASRIATGHTLGDQAETVLLRLLRGAGARGLAAIPPRRGP
VIRPLIDRTREEGNAYLRAMGLGWADDPTNAVPGYARNRLRLELWPLLRAAAPAADRALARAADLAREDERALDRRARAV
AGGGTAVDVAALRGEPLAVRRRVVRRLWRAATGSGRALEAQHVAAVLALLRRGRPGEVALPGGRRARCRYGRLELAGPAA
PVPVVAPVEVPGPGRYALPALDAWVEVGAARPAEVPWPLTLRTRRPGDRFRPEGGPGGTKLKRWLIDRKVPREARDRLVL
LAAPGGAVLAVPELGAVAEGLGPSGAGLRVRISTAPGPDGSYCKGGEGLL

Sequences:

>Translated_450_residues
MRRPDLHPYPAAVLETARRRRLFGPADRVLVALSGGADSTALLAALAALRDAGALAEVRALYLDHGLRAGSEPEAASARA
ACARLGVPLEERRLAVGAGNVQAEARRVRYAALREAAARAGASRIATGHTLGDQAETVLLRLLRGAGARGLAAIPPRRGP
VIRPLIDRTREEGNAYLRAMGLGWADDPTNAVPGYARNRLRLELWPLLRAAAPAADRALARAADLAREDERALDRRARAV
AGGGTAVDVAALRGEPLAVRRRVVRRLWRAATGSGRALEAQHVAAVLALLRRGRPGEVALPGGRRARCRYGRLELAGPAA
PVPVVAPVEVPGPGRYALPALDAWVEVGAARPAEVPWPLTLRTRRPGDRFRPEGGPGGTKLKRWLIDRKVPREARDRLVL
LAAPGGAVLAVPELGAVAEGLGPSGAGLRVRISTAPGPDGSYCKGGEGLL
>Mature_450_residues
MRRPDLHPYPAAVLETARRRRLFGPADRVLVALSGGADSTALLAALAALRDAGALAEVRALYLDHGLRAGSEPEAASARA
ACARLGVPLEERRLAVGAGNVQAEARRVRYAALREAAARAGASRIATGHTLGDQAETVLLRLLRGAGARGLAAIPPRRGP
VIRPLIDRTREEGNAYLRAMGLGWADDPTNAVPGYARNRLRLELWPLLRAAAPAADRALARAADLAREDERALDRRARAV
AGGGTAVDVAALRGEPLAVRRRVVRRLWRAATGSGRALEAQHVAAVLALLRRGRPGEVALPGGRRARCRYGRLELAGPAA
PVPVVAPVEVPGPGRYALPALDAWVEVGAARPAEVPWPLTLRTRRPGDRFRPEGGPGGTKLKRWLIDRKVPREARDRLVL
LAAPGGAVLAVPELGAVAEGLGPSGAGLRVRISTAPGPDGSYCKGGEGLL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=209, Percent_Identity=37.3205741626794, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 47370; Mature: 47370

Theoretical pI: Translated: 11.88; Mature: 11.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPDLHPYPAAVLETARRRRLFGPADRVLVALSGGADSTALLAALAALRDAGALAEVRA
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHH
LYLDHGLRAGSEPEAASARAACARLGVPLEERRLAVGAGNVQAEARRVRYAALREAAARA
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHC
GASRIATGHTLGDQAETVLLRLLRGAGARGLAAIPPRRGPVIRPLIDRTREEGNAYLRAM
CHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCEEEEEE
GLGWADDPTNAVPGYARNRLRLELWPLLRAAAPAADRALARAADLAREDERALDRRARAV
CCCCCCCCCCCCCCHHHCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGGTAVDVAALRGEPLAVRRRVVRRLWRAATGSGRALEAQHVAAVLALLRRGRPGEVAL
CCCCCEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCEEC
PGGRRARCRYGRLELAGPAAPVPVVAPVEVPGPGRYALPALDAWVEVGAARPAEVPWPLT
CCCCCCCEECCCEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEE
LRTRRPGDRFRPEGGPGGTKLKRWLIDRKVPREARDRLVLLAAPGGAVLAVPELGAVAEG
EECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCEEEEECCCCCEEECCCHHHHHHC
LGPSGAGLRVRISTAPGPDGSYCKGGEGLL
CCCCCCCEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRPDLHPYPAAVLETARRRRLFGPADRVLVALSGGADSTALLAALAALRDAGALAEVRA
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHH
LYLDHGLRAGSEPEAASARAACARLGVPLEERRLAVGAGNVQAEARRVRYAALREAAARA
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHC
GASRIATGHTLGDQAETVLLRLLRGAGARGLAAIPPRRGPVIRPLIDRTREEGNAYLRAM
CHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCEEEEEE
GLGWADDPTNAVPGYARNRLRLELWPLLRAAAPAADRALARAADLAREDERALDRRARAV
CCCCCCCCCCCCCCHHHCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGGTAVDVAALRGEPLAVRRRVVRRLWRAATGSGRALEAQHVAAVLALLRRGRPGEVAL
CCCCCEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCEEC
PGGRRARCRYGRLELAGPAAPVPVVAPVEVPGPGRYALPALDAWVEVGAARPAEVPWPLT
CCCCCCCEECCCEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEE
LRTRRPGDRFRPEGGPGGTKLKRWLIDRKVPREARDRLVLLAAPGGAVLAVPELGAVAEG
EECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCEEEEECCCCCEEECCCHHHHHHC
LGPSGAGLRVRISTAPGPDGSYCKGGEGLL
CCCCCCCEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]