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The map label for this gene is hybA [C]

Identifier: 220917060

GI number: 220917060

Start: 2210520

End: 2211422

Strand: Direct

Name: hybA [C]

Synonym: A2cp1_1956

Alternate gene names: 220917060

Gene position: 2210520-2211422 (Clockwise)

Preceding gene: 220917059

Following gene: 220917061

Centisome position: 43.95

GC content: 70.43

Gene sequence:

>903_bases
ATGAAGATCGGACGCAGAGGGTTCTTCAAGATCGCGGCAGTGACGGCGGGGGCCGCCGGCGCGTCGGCGGCGACCGCCGC
CGCAGCGGCGCACGGGGCGGCGGAGGACGGGCCAGGCGTGCTCGTCGACACGACCCTCTGCGTCGGCTGCCGCGCGTGCG
AGGCGGCGTGCGCCGAGGCCAACCACCTCGCGCCGCCGCCCGAGGACGCGAACGTCTTCGACCAGAAGCGCGACATGCAC
GAGCAGGCCTTCACGGTGGTGAACCGCACCGAGAAGGCCCCCGGCATCGAGCGCTTCGCGAAGAAGCAGTGCATGCACTG
CCTGGCGCCGGGCTGCGCCTCGGCGTGCCCGGTCAAGGCGATGTCCAAGTCGCCCGAGGGGCCGGTGGTCTACAACCCGA
ACCGCTGCATGGGCTGCCGGTACTGCATGATCGCCTGCCCGTTCGACGTGCCGAAGTACGAGTACGGCTCGAACGCGCCG
CGCGTGCGCAAGTGCAGCTTCTGCGCAGAGCGGCAGGCGAAGGGCCTCAAGCCGGCGTGCACCGAGGTGTGCCCCTCGGG
CGCGCTCACCTTCGGGAAGCGCAGCGAGCTCCTCGAGGCCGCCAAGACCCGCATCTACACGAACCCCGGCCGTTACGTGC
ACCACGTGTACGGCGAGCACGAGGCCGGCGGGACGAGCTGGCTGTACATCACCGACGTCGACATGGAGCAGCTCGCGTTC
CGGACCGACGTCCCCGAGCGGCCCATCCCGGACCTCGCCTCCGGCGCGCTGTCGGTGCCGCCGTTCGTGATGACGCTCTG
GCCGCCGCTGCTGATGGGCATCTACGCGTTCTCCAACCGGCGCGAGGAGATCAAGAACGCCGAGTCGGAGCCGTCGAACG
GGAAGGAGGAGCACCATGGCTAA

Upstream 100 bases:

>100_bases
ATGATGTTCGCATTCGTCGTGATGGTGGTCGGAGTTGCGGTGCTCGGGACCATGAACCTCGTCGCGGCGCACAGCTAGCC
GCACCCGCGCGGAGACAACC

Downstream 100 bases:

>100_bases
CGCCACCGCGGTCGCCCAGCACTCGTTCGTCCGCGAGAAGATCCTGCTCGGGATGAGCTTGAAGGAGTACCTCCGCGCCC
AGCTCACGCCGACGAACGCC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKIGRRGFFKIAAVTAGAAGASAATAAAAAHGAAEDGPGVLVDTTLCVGCRACEAACAEANHLAPPPEDANVFDQKRDMH
EQAFTVVNRTEKAPGIERFAKKQCMHCLAPGCASACPVKAMSKSPEGPVVYNPNRCMGCRYCMIACPFDVPKYEYGSNAP
RVRKCSFCAERQAKGLKPACTEVCPSGALTFGKRSELLEAAKTRIYTNPGRYVHHVYGEHEAGGTSWLYITDVDMEQLAF
RTDVPERPIPDLASGALSVPPFVMTLWPPLLMGIYAFSNRREEIKNAESEPSNGKEEHHG

Sequences:

>Translated_300_residues
MKIGRRGFFKIAAVTAGAAGASAATAAAAAHGAAEDGPGVLVDTTLCVGCRACEAACAEANHLAPPPEDANVFDQKRDMH
EQAFTVVNRTEKAPGIERFAKKQCMHCLAPGCASACPVKAMSKSPEGPVVYNPNRCMGCRYCMIACPFDVPKYEYGSNAP
RVRKCSFCAERQAKGLKPACTEVCPSGALTFGKRSELLEAAKTRIYTNPGRYVHHVYGEHEAGGTSWLYITDVDMEQLAF
RTDVPERPIPDLASGALSVPPFVMTLWPPLLMGIYAFSNRREEIKNAESEPSNGKEEHHG
>Mature_300_residues
MKIGRRGFFKIAAVTAGAAGASAATAAAAAHGAAEDGPGVLVDTTLCVGCRACEAACAEANHLAPPPEDANVFDQKRDMH
EQAFTVVNRTEKAPGIERFAKKQCMHCLAPGCASACPVKAMSKSPEGPVVYNPNRCMGCRYCMIACPFDVPKYEYGSNAP
RVRKCSFCAERQAKGLKPACTEVCPSGALTFGKRSELLEAAKTRIYTNPGRYVHHVYGEHEAGGTSWLYITDVDMEQLAF
RTDVPERPIPDLASGALSVPPFVMTLWPPLLMGIYAFSNRREEIKNAESEPSNGKEEHHG

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=337, Percent_Identity=33.5311572700297, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1787749, Length=273, Percent_Identity=32.2344322344322, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1790326, Length=202, Percent_Identity=36.1386138613861, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787122, Length=159, Percent_Identity=34.5911949685535, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI2367345, Length=234, Percent_Identity=32.9059829059829, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI226510944, Length=230, Percent_Identity=30, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI2367245, Length=158, Percent_Identity=31.6455696202532, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788811, Length=170, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082179, Length=171, Percent_Identity=30.4093567251462, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082180, Length=166, Percent_Identity=30.7228915662651, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789079, Length=197, Percent_Identity=26.3959390862944, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 32298; Mature: 32298

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGRRGFFKIAAVTAGAAGASAATAAAAAHGAAEDGPGVLVDTTLCVGCRACEAACAEA
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHCC
NHLAPPPEDANVFDQKRDMHEQAFTVVNRTEKAPGIERFAKKQCMHCLAPGCASACPVKA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHH
MSKSPEGPVVYNPNRCMGCRYCMIACPFDVPKYEYGSNAPRVRKCSFCAERQAKGLKPAC
HCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
TEVCPSGALTFGKRSELLEAAKTRIYTNPGRYVHHVYGEHEAGGTSWLYITDVDMEQLAF
HHHCCCCCCCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHH
RTDVPERPIPDLASGALSVPPFVMTLWPPLLMGIYAFSNRREEIKNAESEPSNGKEEHHG
HCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIGRRGFFKIAAVTAGAAGASAATAAAAAHGAAEDGPGVLVDTTLCVGCRACEAACAEA
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHCC
NHLAPPPEDANVFDQKRDMHEQAFTVVNRTEKAPGIERFAKKQCMHCLAPGCASACPVKA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHH
MSKSPEGPVVYNPNRCMGCRYCMIACPFDVPKYEYGSNAPRVRKCSFCAERQAKGLKPAC
HCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
TEVCPSGALTFGKRSELLEAAKTRIYTNPGRYVHHVYGEHEAGGTSWLYITDVDMEQLAF
HHHCCCCCCCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHH
RTDVPERPIPDLASGALSVPPFVMTLWPPLLMGIYAFSNRREEIKNAESEPSNGKEEHHG
HCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]