Click here to switch to the map view.

The map label for this gene is cysL [H]

Identifier: 220916855

GI number: 220916855

Start: 1956677

End: 1957579

Strand: Direct

Name: cysL [H]

Synonym: A2cp1_1751

Alternate gene names: 220916855

Gene position: 1956677-1957579 (Clockwise)

Preceding gene: 220916853

Following gene: 220916856

Centisome position: 38.91

GC content: 75.42

Gene sequence:

>903_bases
GTGCGCCGCCTCGATCCCCGCAAGCTCGAGACCTTCCGCGTGGTCGCGCAGGCCCGGAAGATCTCCACCGCCGCCAAGCT
GCTCCACCTGTCGCAGCCGGCGGTGACCGCGCAGATCCGCGCGCTCGAGGAGGAGTGCGGCCGCGCGCTGCTGGTCCGCT
CGTCGAAGGGCGTCACCCCCAACGACTGGGGGCTGCGGCTGCTCGACACCGCGAAGCAGGTGCACGCCCTGCTCGGCGAG
GTGGAGGGCGCGTTCCAGGACGAGCCGGGCGTGGGCGAGGAGGTGGTGCTCGGCGCGAGCATGACCACCGCCTCCTACGT
CGTCCCGCCGCTCGTCGCCGGCTACCGGGCGGTGCACGGGCGCGCCCCGTTCCGGATGCAGGTCGCGAACACCGCGCGCG
TGCTGGAGTGGGTGGCCGACGGGAAGGTGCCGCTCGGGATCGTGGAGGGCCGGCTGCGCTCGCCCCGCGTCCACCTGGAG
CGATACCTGGAGGACGAGCTGGTGGCGGTCGCCGCCGCGTCCGCCCGCGATCTCCTGCGCATCTCCCGCGCGGCCGACCT
CGCCTCGGCCCCGCTGCTGCTGCGCGAGCCCGGGTCCAACACCCGCGCGCTGGTGGAGGAGGCGCTGGCCGGCGTCCTCG
GGGCTCGCTCGGTGCGGCGGTCGGAGCTCCTGTTCGGCTCCAACCAGTCGATCAAGATGGCCGCGGTGGCGGGGCTGGGC
GTCGCGTTCGTCTCGCGCTGGAGCGTCCAGCTGGAGGTGGCCGCCGGCACGCTGCGCATCCTTCCGCTGCGCGACCTGCG
GCTCACCCGGTCCTTCTCCTGGGCGACCGCCTCGCCCCGCCTCCAGGGGGCCGCCGGACGGTTCCAGGCCTGGGCCCGCC
AGCACCCGCCGCCCAGGCCCTAG

Upstream 100 bases:

>100_bases
CGCCCGCGGGCGCGCCGCGCCTCCACGCAAGATCGGATGGACCATAGGCAGAGCTGATGGCCGGCTCCCGGCCGCGCAGG
GGATCTGATAGCCTGCGCGG

Downstream 100 bases:

>100_bases
CCCGGCGCGGAGCCGGGTCCCCGCGGCGCTTTGTCGGCGCCGGCCGATGGTGTATGGCTACGGCCCCATGAAGACCCTGA
CCGAGCTGAGCGGCACCCTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRRLDPRKLETFRVVAQARKISTAAKLLHLSQPAVTAQIRALEEECGRALLVRSSKGVTPNDWGLRLLDTAKQVHALLGE
VEGAFQDEPGVGEEVVLGASMTTASYVVPPLVAGYRAVHGRAPFRMQVANTARVLEWVADGKVPLGIVEGRLRSPRVHLE
RYLEDELVAVAAASARDLLRISRAADLASAPLLLREPGSNTRALVEEALAGVLGARSVRRSELLFGSNQSIKMAAVAGLG
VAFVSRWSVQLEVAAGTLRILPLRDLRLTRSFSWATASPRLQGAAGRFQAWARQHPPPRP

Sequences:

>Translated_300_residues
MRRLDPRKLETFRVVAQARKISTAAKLLHLSQPAVTAQIRALEEECGRALLVRSSKGVTPNDWGLRLLDTAKQVHALLGE
VEGAFQDEPGVGEEVVLGASMTTASYVVPPLVAGYRAVHGRAPFRMQVANTARVLEWVADGKVPLGIVEGRLRSPRVHLE
RYLEDELVAVAAASARDLLRISRAADLASAPLLLREPGSNTRALVEEALAGVLGARSVRRSELLFGSNQSIKMAAVAGLG
VAFVSRWSVQLEVAAGTLRILPLRDLRLTRSFSWATASPRLQGAAGRFQAWARQHPPPRP
>Mature_300_residues
MRRLDPRKLETFRVVAQARKISTAAKLLHLSQPAVTAQIRALEEECGRALLVRSSKGVTPNDWGLRLLDTAKQVHALLGE
VEGAFQDEPGVGEEVVLGASMTTASYVVPPLVAGYRAVHGRAPFRMQVANTARVLEWVADGKVPLGIVEGRLRSPRVHLE
RYLEDELVAVAAASARDLLRISRAADLASAPLLLREPGSNTRALVEEALAGVLGARSVRRSELLFGSNQSIKMAAVAGLG
VAFVSRWSVQLEVAAGTLRILPLRDLRLTRSFSWATASPRLQGAAGRFQAWARQHPPPRP

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=31.5436241610738, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790399, Length=252, Percent_Identity=26.1904761904762, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790208, Length=145, Percent_Identity=28.9655172413793, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32513; Mature: 32513

Theoretical pI: Translated: 11.41; Mature: 11.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLDPRKLETFRVVAQARKISTAAKLLHLSQPAVTAQIRALEEECGRALLVRSSKGVTP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
NDWGLRLLDTAKQVHALLGEVEGAFQDEPGVGEEVVLGASMTTASYVVPPLVAGYRAVHG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCC
RAPFRMQVANTARVLEWVADGKVPLGIVEGRLRSPRVHLERYLEDELVAVAAASARDLLR
CCCEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ISRAADLASAPLLLREPGSNTRALVEEALAGVLGARSVRRSELLFGSNQSIKMAAVAGLG
HHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHH
VAFVSRWSVQLEVAAGTLRILPLRDLRLTRSFSWATASPRLQGAAGRFQAWARQHPPPRP
HHHHHHCEEEEEEECCEEEEEEECHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRRLDPRKLETFRVVAQARKISTAAKLLHLSQPAVTAQIRALEEECGRALLVRSSKGVTP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
NDWGLRLLDTAKQVHALLGEVEGAFQDEPGVGEEVVLGASMTTASYVVPPLVAGYRAVHG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCC
RAPFRMQVANTARVLEWVADGKVPLGIVEGRLRSPRVHLERYLEDELVAVAAASARDLLR
CCCEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ISRAADLASAPLLLREPGSNTRALVEEALAGVLGARSVRRSELLFGSNQSIKMAAVAGLG
HHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHH
VAFVSRWSVQLEVAAGTLRILPLRDLRLTRSFSWATASPRLQGAAGRFQAWARQHPPPRP
HHHHHHCEEEEEEECCEEEEEEECHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]