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The map label for this gene is miaB [H]

Identifier: 220916715

GI number: 220916715

Start: 1798720

End: 1800114

Strand: Direct

Name: miaB [H]

Synonym: A2cp1_1609

Alternate gene names: 220916715

Gene position: 1798720-1800114 (Clockwise)

Preceding gene: 220916714

Following gene: 220916716

Centisome position: 35.76

GC content: 74.41

Gene sequence:

>1395_bases
ATGTCCGATCTCGTGCCCCTCTCCAGGAAGCCGGCCCCCGTCGCGGGCGACCCCGTCCCGTCGCCGGCGGCGCCGCCGCG
CAAGGTCTACGTCCACACCTTCGGCTGCCAGATGAACGAGTCCGACTCCGACCGGATGGTCGAGCTGCTCGGGCGCCACG
CCTACGCCCGCGCCGCCAGCGCGGACGAGGCCGACCTCATCCTGCTCAACACCTGCGCGGTGCGCGAGAAGGCCGAGCAG
AAGCTGCTCTCGGCGCTGGGGCGCTATCGCGAGGTGAAGGCGCGGCGCGGCGCGCTCATCGCGGTGTCCGGGTGCGTCGC
GCAGCAGGAGAAGGACCGGCTGCTCGCGCGCGTGCCGTACGTGGACTTCGTGTTCGGGCCGGACAACATCGGCCGGCTCC
CGGAGATGGTCGAGCGCGCCCGCGACGAGCGCTTCGCCGAGACCGGCTGGATGGACTCGGAGGAGTACGTGTTCCCGCGC
GCCGACCCCGAGGCGGCCCGCGGCCGGGTGACCGCGTTCGTCACCGCCATGAAGGGCTGCGACAACGTCTGCGCGTTCTG
CATCGTGCCGCACACCCGCGGGCGCGAGGTGTCGCGGCCGTTCCCCGACGTGGTGGCCGAGTGCGCGGCGCTCGCCGGGG
TGGGCGTGCGCGAGGTGACGCTCATCGGGCAGAACGTGAACTCGTACACCGGCGGCTGCACGTTCGCCGACCTGCTCCGG
CGCGTGGCGGCCGTGCCCGGGATCGACCGCATCCGCTTCACCACCAGCCACCCGCACGACCTGTCCGACGCGCTCGTCGA
GGTGTTCCGCGACGAGCCGAAGGTGATGCCGCACTTCCACCTCCCGGTGCAGTCCGGCTCCGACGCCGTGCTGCGCCGCA
TGCGCCGCGACTACTCGGTGGCCGAGTACCTCGATCGCTTCGACCGCCTGCGCGCCGCCCGGCCCGGCATCGCCATCACC
ACCGACTTCATCGTCGGGTTCCCCGGCGAGACCGACGCCGACTTCGAGGCCTCGCTGGCGCTGCTGGAGCGCGCGCGCTT
CGAGCAGAGCTTCAGCTTCGTGTTCAGCCCGCGGCCGCACACCGTCGCGGCGGTGCGGCTCGGCAGCGCGCCGGAGTGGC
AGGACGTGCCGCGCGGGACCGCGGTGGCGCGGCTCGAGCGGCTGCTCGCGGCGCAGCGGCGCATCGCCGGGGAGATCCTG
GCCGGGGAGCTCGGGAAGGTGGTCGAGGTGCTGGTGGAGGGCCCGTCCGACGAGCCGGGGGAGCGGCTCGGGCGGACGCC
CGAGAACCGCGTGGTCCACCTGGCGGCGGACGAGGCGGCCGCGCCGGCGGGCGCGCGCGTGGCGGTGCGCATCACGCGCG
CCGGCGGCAGCTCGCTCTCGGGCACGCTCGCGTGA

Upstream 100 bases:

>100_bases
ACGCGCTGGTGCCCGCCCGCTGACCCGCCGGCCCGCCCCCCGCCGTGCCCGGCCGCCCGGCGATTGACTCCCCGCCCACC
GTTCCATAGGTTCCGCGCCG

Downstream 100 bases:

>100_bases
GCGCGGACCTCCCGGCCGCGGCTGGGGCCGTCGCCGCCCCCGTGGGGAGGGGCTGGCGGGCGCTCGCGGCCGCGGGCGCG
GCCGCGTACGCCGGGTTCAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSDLVPLSRKPAPVAGDPVPSPAAPPRKVYVHTFGCQMNESDSDRMVELLGRHAYARAASADEADLILLNTCAVREKAEQ
KLLSALGRYREVKARRGALIAVSGCVAQQEKDRLLARVPYVDFVFGPDNIGRLPEMVERARDERFAETGWMDSEEYVFPR
ADPEAARGRVTAFVTAMKGCDNVCAFCIVPHTRGREVSRPFPDVVAECAALAGVGVREVTLIGQNVNSYTGGCTFADLLR
RVAAVPGIDRIRFTTSHPHDLSDALVEVFRDEPKVMPHFHLPVQSGSDAVLRRMRRDYSVAEYLDRFDRLRAARPGIAIT
TDFIVGFPGETDADFEASLALLERARFEQSFSFVFSPRPHTVAAVRLGSAPEWQDVPRGTAVARLERLLAAQRRIAGEIL
AGELGKVVEVLVEGPSDEPGERLGRTPENRVVHLAADEAAAPAGARVAVRITRAGGSSLSGTLA

Sequences:

>Translated_464_residues
MSDLVPLSRKPAPVAGDPVPSPAAPPRKVYVHTFGCQMNESDSDRMVELLGRHAYARAASADEADLILLNTCAVREKAEQ
KLLSALGRYREVKARRGALIAVSGCVAQQEKDRLLARVPYVDFVFGPDNIGRLPEMVERARDERFAETGWMDSEEYVFPR
ADPEAARGRVTAFVTAMKGCDNVCAFCIVPHTRGREVSRPFPDVVAECAALAGVGVREVTLIGQNVNSYTGGCTFADLLR
RVAAVPGIDRIRFTTSHPHDLSDALVEVFRDEPKVMPHFHLPVQSGSDAVLRRMRRDYSVAEYLDRFDRLRAARPGIAIT
TDFIVGFPGETDADFEASLALLERARFEQSFSFVFSPRPHTVAAVRLGSAPEWQDVPRGTAVARLERLLAAQRRIAGEIL
AGELGKVVEVLVEGPSDEPGERLGRTPENRVVHLAADEAAAPAGARVAVRITRAGGSSLSGTLA
>Mature_463_residues
SDLVPLSRKPAPVAGDPVPSPAAPPRKVYVHTFGCQMNESDSDRMVELLGRHAYARAASADEADLILLNTCAVREKAEQK
LLSALGRYREVKARRGALIAVSGCVAQQEKDRLLARVPYVDFVFGPDNIGRLPEMVERARDERFAETGWMDSEEYVFPRA
DPEAARGRVTAFVTAMKGCDNVCAFCIVPHTRGREVSRPFPDVVAECAALAGVGVREVTLIGQNVNSYTGGCTFADLLRR
VAAVPGIDRIRFTTSHPHDLSDALVEVFRDEPKVMPHFHLPVQSGSDAVLRRMRRDYSVAEYLDRFDRLRAARPGIAITT
DFIVGFPGETDADFEASLALLERARFEQSFSFVFSPRPHTVAAVRLGSAPEWQDVPRGTAVARLERLLAAQRRIAGEILA
GELGKVVEVLVEGPSDEPGERLGRTPENRVVHLAADEAAAPAGARVAVRITRAGGSSLSGTLA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=35.1464435146443, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=35.1464435146443, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI93277076, Length=358, Percent_Identity=31.5642458100559, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=45.2115812917595, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1787057, Length=462, Percent_Identity=27.7056277056277, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=433, Percent_Identity=34.1801385681293, Blast_Score=237, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI71996771, Length=260, Percent_Identity=32.3076923076923, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=37.319587628866, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI19922432, Length=471, Percent_Identity=30.1486199575372, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50532; Mature: 50401

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLVPLSRKPAPVAGDPVPSPAAPPRKVYVHTFGCQMNESDSDRMVELLGRHAYARAAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
ADEADLILLNTCAVREKAEQKLLSALGRYREVKARRGALIAVSGCVAQQEKDRLLARVPY
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCC
VDFVFGPDNIGRLPEMVERARDERFAETGWMDSEEYVFPRADPEAARGRVTAFVTAMKGC
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHCHHHHHHHHHHCC
DNVCAFCIVPHTRGREVSRPFPDVVAECAALAGVGVREVTLIGQNVNSYTGGCTFADLLR
CCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHH
RVAAVPGIDRIRFTTSHPHDLSDALVEVFRDEPKVMPHFHLPVQSGSDAVLRRMRRDYSV
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCH
AEYLDRFDRLRAARPGIAITTDFIVGFPGETDADFEASLALLERARFEQSFSFVFSPRPH
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCC
TVAAVRLGSAPEWQDVPRGTAVARLERLLAAQRRIAGEILAGELGKVVEVLVEGPSDEPG
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ERLGRTPENRVVHLAADEAAAPAGARVAVRITRAGGSSLSGTLA
HHHCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SDLVPLSRKPAPVAGDPVPSPAAPPRKVYVHTFGCQMNESDSDRMVELLGRHAYARAAS
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
ADEADLILLNTCAVREKAEQKLLSALGRYREVKARRGALIAVSGCVAQQEKDRLLARVPY
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCC
VDFVFGPDNIGRLPEMVERARDERFAETGWMDSEEYVFPRADPEAARGRVTAFVTAMKGC
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHCHHHHHHHHHHCC
DNVCAFCIVPHTRGREVSRPFPDVVAECAALAGVGVREVTLIGQNVNSYTGGCTFADLLR
CCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHH
RVAAVPGIDRIRFTTSHPHDLSDALVEVFRDEPKVMPHFHLPVQSGSDAVLRRMRRDYSV
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCH
AEYLDRFDRLRAARPGIAITTDFIVGFPGETDADFEASLALLERARFEQSFSFVFSPRPH
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCC
TVAAVRLGSAPEWQDVPRGTAVARLERLLAAQRRIAGEILAGELGKVVEVLVEGPSDEPG
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ERLGRTPENRVVHLAADEAAAPAGARVAVRITRAGGSSLSGTLA
HHHCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA