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The map label for this gene is rlmI [H]

Identifier: 220916689

GI number: 220916689

Start: 1770598

End: 1771800

Strand: Direct

Name: rlmI [H]

Synonym: A2cp1_1583

Alternate gene names: 220916689

Gene position: 1770598-1771800 (Clockwise)

Preceding gene: 220916688

Following gene: 220916693

Centisome position: 35.21

GC content: 75.23

Gene sequence:

>1203_bases
ATGAAGGCCGAGGCCGAGCTGCCGCTGCGCAAGGACCTGGCGCGCCACCTGCGCGCCGGGCACCCCTGGGTGTTCCGCAA
GGCGGTGGAGCGCGCGCCGGCGCGGCTGCCGGCGGGCACGATCGTGGACGTCACCGAGGACGGCCGCTTCGTGGCCCGCG
GCTACTACGATCCGCACTCCGCCATCTCGGTCCGCATCCTCACCCGCGAGCCGGCCGAGGCCATCGACGCCGCGTTCTGG
CGCCGGCGCGTGGCGCGGGCGGTGGCGCTGCGGAAGGAGCTGGTGCGCGACACCACCGGCTACCGGGTGGTGCACGGCGA
GGGCGACGCGCTGCCCGGCGTCGTCGCCGATCGCTACGACCGCCACGTGGTGCTGAAGCTCTACTCCGCCGGCCTCACCC
CGCACCGCGCCGAGATCGTCGAGGCGCTCCACGCCGAGCAGGAGGGCGTGGCCGGCGTGTTCGGCCGCGACGAGATCCCG
CGCGACGACCAGGACGAGGGCGAGGGCGACGGGAGCGGGCGCGTGCTGTGGGGCGCCGAGCCGCCGGAGCGGATCGCGAT
CGACGAGCACGGGATGAAGGTGCTGGTGGACGTGCGCCGCGGGCAGAAGACCGGCCACTTCCTCGACCAGCGCGAGAACC
GGCGCATGGTGCGCGACCTGGCCCGCGGGCGGGCCGAGGCGCTCAACCTGTTCGGCTACACCGGCGGCTTCTCGGTGGCG
GCGGCGCTGGGCGGCGCCGGGCACGTGGTCACGGTGGACGTGGACCGCGACGCCATCGCGCTCGCGCGCGAGAACTTCCG
CGCCAACGGCCTCGACGCGGCCGACCACGCGTTCGCCGCCGAGGACTGCTTCGAGCTGCTCGCCCGCTACAAGCGCGAGG
GGCGCCGCTTCGACCTGGTGGTGTGCGATCCGCCCGCGTTCGCGAAGTCGCAGAAGGCGGTGGAGGGCGCGCTGGCCGGC
TACGCCTCGCTGAACCGGGCCGCGCTCGCCGTGCTCGCGCCGGGCGGGCTGCTCGTCACCGCGAGCTGCTCGGCGCGCGT
CTCGGCGGAGCAGTTCGCCGACGCGGTCAAGGAGGCGGCCTACAAGGCGCGGGTGGACCTCGCGCTGGTGGCCGAGACCC
GGCAGCCGCCCGATCACCCGGTCTCGCTGCAGTTCCGCGAGGGCCGCTACCTGAAGTGCATGGTGCTGCGCCGCGCGGGG
TAG

Upstream 100 bases:

>100_bases
GGCCGGGGCTGGTGGTGGGCAACTTCTCCCGCGCGCGGAGCGAGCGCGGGGCGCGGCGACGGCCGGCCTCGCCGGCCCGG
CGCCGCCGCACGGAGGCGAG

Downstream 100 bases:

>100_bases
CTACCGGCGCGACGGCGGGGCGTGCCCGCCGAGCGCGCCGGCGAGCGCCTCGGGCTCGTCCAGCGTCACCTCCACGAAGC
CCTGCGCCGGCCGCGCCGAG

Product: PUA domain containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKAEAELPLRKDLARHLRAGHPWVFRKAVERAPARLPAGTIVDVTEDGRFVARGYYDPHSAISVRILTREPAEAIDAAFW
RRRVARAVALRKELVRDTTGYRVVHGEGDALPGVVADRYDRHVVLKLYSAGLTPHRAEIVEALHAEQEGVAGVFGRDEIP
RDDQDEGEGDGSGRVLWGAEPPERIAIDEHGMKVLVDVRRGQKTGHFLDQRENRRMVRDLARGRAEALNLFGYTGGFSVA
AALGGAGHVVTVDVDRDAIALARENFRANGLDAADHAFAAEDCFELLARYKREGRRFDLVVCDPPAFAKSQKAVEGALAG
YASLNRAALAVLAPGGLLVTASCSARVSAEQFADAVKEAAYKARVDLALVAETRQPPDHPVSLQFREGRYLKCMVLRRAG

Sequences:

>Translated_400_residues
MKAEAELPLRKDLARHLRAGHPWVFRKAVERAPARLPAGTIVDVTEDGRFVARGYYDPHSAISVRILTREPAEAIDAAFW
RRRVARAVALRKELVRDTTGYRVVHGEGDALPGVVADRYDRHVVLKLYSAGLTPHRAEIVEALHAEQEGVAGVFGRDEIP
RDDQDEGEGDGSGRVLWGAEPPERIAIDEHGMKVLVDVRRGQKTGHFLDQRENRRMVRDLARGRAEALNLFGYTGGFSVA
AALGGAGHVVTVDVDRDAIALARENFRANGLDAADHAFAAEDCFELLARYKREGRRFDLVVCDPPAFAKSQKAVEGALAG
YASLNRAALAVLAPGGLLVTASCSARVSAEQFADAVKEAAYKARVDLALVAETRQPPDHPVSLQFREGRYLKCMVLRRAG
>Mature_400_residues
MKAEAELPLRKDLARHLRAGHPWVFRKAVERAPARLPAGTIVDVTEDGRFVARGYYDPHSAISVRILTREPAEAIDAAFW
RRRVARAVALRKELVRDTTGYRVVHGEGDALPGVVADRYDRHVVLKLYSAGLTPHRAEIVEALHAEQEGVAGVFGRDEIP
RDDQDEGEGDGSGRVLWGAEPPERIAIDEHGMKVLVDVRRGQKTGHFLDQRENRRMVRDLARGRAEALNLFGYTGGFSVA
AALGGAGHVVTVDVDRDAIALARENFRANGLDAADHAFAAEDCFELLARYKREGRRFDLVVCDPPAFAKSQKAVEGALAG
YASLNRAALAVLAPGGLLVTASCSARVSAEQFADAVKEAAYKARVDLALVAETRQPPDHPVSLQFREGRYLKCMVLRRAG

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=394, Percent_Identity=38.0710659898477, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI1787181, Length=133, Percent_Identity=37.593984962406, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43762; Mature: 43762

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAEAELPLRKDLARHLRAGHPWVFRKAVERAPARLPAGTIVDVTEDGRFVARGYYDPHS
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCEEEECCCCCCC
AISVRILTREPAEAIDAAFWRRRVARAVALRKELVRDTTGYRVVHGEGDALPGVVADRYD
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCC
RHVVLKLYSAGLTPHRAEIVEALHAEQEGVAGVFGRDEIPRDDQDEGEGDGSGRVLWGAE
CEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCC
PPERIAIDEHGMKVLVDVRRGQKTGHFLDQRENRRMVRDLARGRAEALNLFGYTGGFSVA
CCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHH
AALGGAGHVVTVDVDRDAIALARENFRANGLDAADHAFAAEDCFELLARYKREGRRFDLV
HHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VCDPPAFAKSQKAVEGALAGYASLNRAALAVLAPGGLLVTASCSARVSAEQFADAVKEAA
EECCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
YKARVDLALVAETRQPPDHPVSLQFREGRYLKCMVLRRAG
HHHHCEEEEEEECCCCCCCCEEEEECCCCEEEEEEEECCC
>Mature Secondary Structure
MKAEAELPLRKDLARHLRAGHPWVFRKAVERAPARLPAGTIVDVTEDGRFVARGYYDPHS
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCEEEECCCCCCC
AISVRILTREPAEAIDAAFWRRRVARAVALRKELVRDTTGYRVVHGEGDALPGVVADRYD
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCC
RHVVLKLYSAGLTPHRAEIVEALHAEQEGVAGVFGRDEIPRDDQDEGEGDGSGRVLWGAE
CEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCC
PPERIAIDEHGMKVLVDVRRGQKTGHFLDQRENRRMVRDLARGRAEALNLFGYTGGFSVA
CCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHH
AALGGAGHVVTVDVDRDAIALARENFRANGLDAADHAFAAEDCFELLARYKREGRRFDLV
HHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VCDPPAFAKSQKAVEGALAGYASLNRAALAVLAPGGLLVTASCSARVSAEQFADAVKEAA
EECCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
YKARVDLALVAETRQPPDHPVSLQFREGRYLKCMVLRRAG
HHHHCEEEEEEECCCCCCCCEEEEECCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA