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The map label for this gene is rpoE [H]

Identifier: 220916687

GI number: 220916687

Start: 1768311

End: 1768955

Strand: Reverse

Name: rpoE [H]

Synonym: A2cp1_1581

Alternate gene names: 220916687

Gene position: 1768955-1768311 (Counterclockwise)

Preceding gene: 220916690

Following gene: 220916686

Centisome position: 35.17

GC content: 65.27

Gene sequence:

>645_bases
ATGTTCGACCTCCGACAGAACGACCTGACCCGCGACGAGTTCCAGGAGCTCGCGCTCAAGCACATCGACCCCCTCTACTC
GGCGGCGCTGCGGCTCACCAAGAACGACCGCGACGCCGAGGATCTCGTCCAGGACACGTTCCTGCGCGCCTACCGCTTCT
TCGACAAGTTCGAGCGCGGCACGAACATCAAGGCGTGGCTGTTCAAGATCCTGACCAACACCTTCATCAACCAGTATCGA
CGGTCGGTGAAGGAGCGCAACATCGTCGAGGGGTCGGAGCGCGACGCCGTTCACGAGCGGTTCGTGTCGCGCGAGGCGGC
CGAGTACGCGGCCAACCCGGAGCAGTACTTCTTCGACCGGCTCCTCTCCGACGACGTCCTGAAGGCGGTGGACTCGCTGC
CCATCGACTTCCGGATGGTCGTGATCCTCGCGGACCTGCAGGAGTTCTCGTACAAGGAGATCGCCGAGATCCTCGACGTC
CCGGTCGGCACGGTGATGAGCCGGCTGTACCGTGGACGCCGGCTGTTGCAGAAGGCGCTGGCGAGCTACGCCGTGGTCTC
CGGGGTCATCCAGCTCCCGGAGTCCGAGGACGGCGAGAGCATCGACCTCGAGACGTACCGGCGCCGCCGCGGCAAGTCGG
CCTAG

Upstream 100 bases:

>100_bases
GCGCGGGAATCTCCCCGGGCGATGGGCCGTTTCCCAGGTCGAGTGGCCTGATTCGGAAGCGGGTGTGCGTCGTCCGGGAT
TAGAAGAGATCAAGCCCGAA

Downstream 100 bases:

>100_bases
GATCCCGGCCAGAGGGCCTCGAATGCGGATCGACTCCACGCTCATCGCCGCCATGGACTGCCGCGAGCTCGAGCGATCGA
TCGACGCGTACCTGGACGGC

Product: ECF subfamily RNA polymerase sigma-24 subunit

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MFDLRQNDLTRDEFQELALKHIDPLYSAALRLTKNDRDAEDLVQDTFLRAYRFFDKFERGTNIKAWLFKILTNTFINQYR
RSVKERNIVEGSERDAVHERFVSREAAEYAANPEQYFFDRLLSDDVLKAVDSLPIDFRMVVILADLQEFSYKEIAEILDV
PVGTVMSRLYRGRRLLQKALASYAVVSGVIQLPESEDGESIDLETYRRRRGKSA

Sequences:

>Translated_214_residues
MFDLRQNDLTRDEFQELALKHIDPLYSAALRLTKNDRDAEDLVQDTFLRAYRFFDKFERGTNIKAWLFKILTNTFINQYR
RSVKERNIVEGSERDAVHERFVSREAAEYAANPEQYFFDRLLSDDVLKAVDSLPIDFRMVVILADLQEFSYKEIAEILDV
PVGTVMSRLYRGRRLLQKALASYAVVSGVIQLPESEDGESIDLETYRRRRGKSA
>Mature_214_residues
MFDLRQNDLTRDEFQELALKHIDPLYSAALRLTKNDRDAEDLVQDTFLRAYRFFDKFERGTNIKAWLFKILTNTFINQYR
RSVKERNIVEGSERDAVHERFVSREAAEYAANPEQYFFDRLLSDDVLKAVDSLPIDFRMVVILADLQEFSYKEIAEILDV
PVGTVMSRLYRGRRLLQKALASYAVVSGVIQLPESEDGESIDLETYRRRRGKSA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=141, Percent_Identity=38.2978723404255, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 25016; Mature: 25016

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDLRQNDLTRDEFQELALKHIDPLYSAALRLTKNDRDAEDLVQDTFLRAYRFFDKFERG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
TNIKAWLFKILTNTFINQYRRSVKERNIVEGSERDAVHERFVSREAAEYAANPEQYFFDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLSDDVLKAVDSLPIDFRMVVILADLQEFSYKEIAEILDVPVGTVMSRLYRGRRLLQKAL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ASYAVVSGVIQLPESEDGESIDLETYRRRRGKSA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MFDLRQNDLTRDEFQELALKHIDPLYSAALRLTKNDRDAEDLVQDTFLRAYRFFDKFERG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
TNIKAWLFKILTNTFINQYRRSVKERNIVEGSERDAVHERFVSREAAEYAANPEQYFFDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLSDDVLKAVDSLPIDFRMVVILADLQEFSYKEIAEILDVPVGTVMSRLYRGRRLLQKAL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ASYAVVSGVIQLPESEDGESIDLETYRRRRGKSA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]