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The map label for this gene is pleD [H]

Identifier: 220916672

GI number: 220916672

Start: 1751094

End: 1752461

Strand: Reverse

Name: pleD [H]

Synonym: A2cp1_1566

Alternate gene names: 220916672

Gene position: 1752461-1751094 (Counterclockwise)

Preceding gene: 220916685

Following gene: 220916670

Centisome position: 34.84

GC content: 73.03

Gene sequence:

>1368_bases
ATGGCCTACTCGGTCCTCCTCGTGGACGACGAGCGCTTCGCGCGCACCGTGTACTCGGACTACCTGCGCGCCGCGGGGTA
CGAGGTCGAGCTCGCGGAGGACGCGGAGGCGGCGCTCGCGCTCCTGCGCCAGCGGCGCTTCGACGTGCTGCTCACCGACG
TGATCCTCCCGGGCTCGTCCGGCCTCGACCTGCTCTCGGCCGCGAAGCAGCTCGACCCGAACCTCGAGGTCACGGTCATC
ACCGCGCTCGACAAGGTGGACCCGGCCGTGCGCGCCATGAAGTCCGGCGCCTCGGACTACCTGGTGAAGCCGGTGACGCC
GGAGCAGCTCCAGCTCGCGCTGCAGCGCTGCCTCGCCACCCGCGAGCTGCTCGCCGAGAACAAGGTGCTGCGCGCGCACC
TCACGCTGTTCGAGACCGGCCAGCGCATCGCCGCCACGCTCGATCGCGACAAGCTGGTGCCGATGGCCCTCGCCTCGGTC
GCGACGGCGGCGCGCAGCCCGGTCGCGGCGCTCATGGAGAAGGCGGCGGACGGGAGCTGGGTGCCCTCCGGCGCGCACGG
GACCGATCACGCGGTCGCGACCGAGCTGCTCGCCGCCTGCCGCGCGGAGCTCGATGCGCTGGGGGCCGAGCCGACCGCCC
GGGGCGAGCATGCGCCGGGCGAGCTGGGGCCGTTCGGGCCGCGCGCGCTGCTCCTGCCGGTCACCGAGGAGGGCGCGCTG
CTCGGGGCCGTGGCGGTGAACGCGCCGGGCCCGCTCGAGCCGGCCGGCGCCGAGGCGGTGGGGTACCTCTGCCGCAACCT
GGGGCTCGCGCTCCGCAACCTCGGCCGGCTGAGGCAGGTCGAGCACCTCGCCTACCTCGACGACCTCACGCACCTCTACA
ACACCCGCTACCTGGACATGGCGCTCGACCGCGAGATGCAGAGCGGCCGCCCGTTCTCGGTCCTGTTCATGGACCTCGAC
CACTTCAAGACCGTCAACGACCAGCACGGCCACCTGTCCGGCTCGCGCCTGCTGGTCGAGGTGGCGCGGGTGCTCCGCTC
CTGCGTCCGCGACGAGGACGTCCTGGTCCGTTACGGGGGGGACGAGTACGTGGTGCTGCTGGTCGGGATCGACTCCGGCG
GCGGGCTGAAGGTGGCCGAGCGGGTCCGCCGGGCCATCGAGGACCACCGGTTCCTGTCACGGGAGGGCGCCCGGGTGCGG
GTCACGGCGTCCATCGGGCTCGCGAGCTACCCCGAGCACGCCCAGGAGAAGAGCGAGATCCTCGACCTCGCCGACCGCGC
CATGTACCGGGGCAAGCGCTCCAGCCGGAACGTGGTCTACATGGCGTCGAAGGACCTGCCGCCGATTCCGGCCGGCGAGA
GGCCGTAA

Upstream 100 bases:

>100_bases
CTGGGCCAGGCGCAGGGCCAGGCGCACACCACCCGCCGAGAACAGGAGCGCCTTGCGGTCCGAGGGGAGCGACACGGGCC
ATGATATACGACGATCCGCG

Downstream 100 bases:

>100_bases
GCGGCCCGGGTTCCGGGCTCGGGCTCGACGGGCTGACCCGAGCGGCAAAAAAAAACGCGGGGCCAAGGAAGGATCCTTGG
CCCCGCTGACGACGACTGCC

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAYSVLLVDDERFARTVYSDYLRAAGYEVELAEDAEAALALLRQRRFDVLLTDVILPGSSGLDLLSAAKQLDPNLEVTVI
TALDKVDPAVRAMKSGASDYLVKPVTPEQLQLALQRCLATRELLAENKVLRAHLTLFETGQRIAATLDRDKLVPMALASV
ATAARSPVAALMEKAADGSWVPSGAHGTDHAVATELLAACRAELDALGAEPTARGEHAPGELGPFGPRALLLPVTEEGAL
LGAVAVNAPGPLEPAGAEAVGYLCRNLGLALRNLGRLRQVEHLAYLDDLTHLYNTRYLDMALDREMQSGRPFSVLFMDLD
HFKTVNDQHGHLSGSRLLVEVARVLRSCVRDEDVLVRYGGDEYVVLLVGIDSGGGLKVAERVRRAIEDHRFLSREGARVR
VTASIGLASYPEHAQEKSEILDLADRAMYRGKRSSRNVVYMASKDLPPIPAGERP

Sequences:

>Translated_455_residues
MAYSVLLVDDERFARTVYSDYLRAAGYEVELAEDAEAALALLRQRRFDVLLTDVILPGSSGLDLLSAAKQLDPNLEVTVI
TALDKVDPAVRAMKSGASDYLVKPVTPEQLQLALQRCLATRELLAENKVLRAHLTLFETGQRIAATLDRDKLVPMALASV
ATAARSPVAALMEKAADGSWVPSGAHGTDHAVATELLAACRAELDALGAEPTARGEHAPGELGPFGPRALLLPVTEEGAL
LGAVAVNAPGPLEPAGAEAVGYLCRNLGLALRNLGRLRQVEHLAYLDDLTHLYNTRYLDMALDREMQSGRPFSVLFMDLD
HFKTVNDQHGHLSGSRLLVEVARVLRSCVRDEDVLVRYGGDEYVVLLVGIDSGGGLKVAERVRRAIEDHRFLSREGARVR
VTASIGLASYPEHAQEKSEILDLADRAMYRGKRSSRNVVYMASKDLPPIPAGERP
>Mature_454_residues
AYSVLLVDDERFARTVYSDYLRAAGYEVELAEDAEAALALLRQRRFDVLLTDVILPGSSGLDLLSAAKQLDPNLEVTVIT
ALDKVDPAVRAMKSGASDYLVKPVTPEQLQLALQRCLATRELLAENKVLRAHLTLFETGQRIAATLDRDKLVPMALASVA
TAARSPVAALMEKAADGSWVPSGAHGTDHAVATELLAACRAELDALGAEPTARGEHAPGELGPFGPRALLLPVTEEGALL
GAVAVNAPGPLEPAGAEAVGYLCRNLGLALRNLGRLRQVEHLAYLDDLTHLYNTRYLDMALDREMQSGRPFSVLFMDLDH
FKTVNDQHGHLSGSRLLVEVARVLRSCVRDEDVLVRYGGDEYVVLLVGIDSGGGLKVAERVRRAIEDHRFLSREGARVRV
TASIGLASYPEHAQEKSEILDLADRAMYRGKRSSRNVVYMASKDLPPIPAGERP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=34.3195266272189, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=37.3493975903614, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=38.2716049382716, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788381, Length=164, Percent_Identity=35.9756097560976, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=29.8780487804878, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=166, Percent_Identity=30.7228915662651, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=32.258064516129, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=35.7142857142857, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=39.1304347826087, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=34.3949044585987, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788085, Length=186, Percent_Identity=27.9569892473118, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=31.2977099236641, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790299, Length=100, Percent_Identity=41, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786599, Length=148, Percent_Identity=29.0540540540541, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788956, Length=120, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081977, Length=163, Percent_Identity=28.2208588957055, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788394, Length=127, Percent_Identity=31.496062992126, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=29.7619047619048, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49349; Mature: 49218

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSVLLVDDERFARTVYSDYLRAAGYEVELAEDAEAALALLRQRRFDVLLTDVILPGSS
CCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHEEHHHCCCCCC
GLDLLSAAKQLDPNLEVTVITALDKVDPAVRAMKSGASDYLVKPVTPEQLQLALQRCLAT
CHHHHHHHHHCCCCCEEEEEEEHHHCCHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHH
RELLAENKVLRAHLTLFETGQRIAATLDRDKLVPMALASVATAARSPVAALMEKAADGSW
HHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCC
VPSGAHGTDHAVATELLAACRAELDALGAEPTARGEHAPGELGPFGPRALLLPVTEEGAL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCE
LGAVAVNAPGPLEPAGAEAVGYLCRNLGLALRNLGRLRQVEHLAYLDDLTHLYNTRYLDM
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ALDREMQSGRPFSVLFMDLDHFKTVNDQHGHLSGSRLLVEVARVLRSCVRDEDVLVRYGG
HHHHHHHCCCCEEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
DEYVVLLVGIDSGGGLKVAERVRRAIEDHRFLSREGARVRVTASIGLASYPEHAQEKSEI
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHH
LDLADRAMYRGKRSSRNVVYMASKDLPPIPAGERP
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AYSVLLVDDERFARTVYSDYLRAAGYEVELAEDAEAALALLRQRRFDVLLTDVILPGSS
CEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHEEHHHCCCCCC
GLDLLSAAKQLDPNLEVTVITALDKVDPAVRAMKSGASDYLVKPVTPEQLQLALQRCLAT
CHHHHHHHHHCCCCCEEEEEEEHHHCCHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHH
RELLAENKVLRAHLTLFETGQRIAATLDRDKLVPMALASVATAARSPVAALMEKAADGSW
HHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCC
VPSGAHGTDHAVATELLAACRAELDALGAEPTARGEHAPGELGPFGPRALLLPVTEEGAL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCE
LGAVAVNAPGPLEPAGAEAVGYLCRNLGLALRNLGRLRQVEHLAYLDDLTHLYNTRYLDM
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ALDREMQSGRPFSVLFMDLDHFKTVNDQHGHLSGSRLLVEVARVLRSCVRDEDVLVRYGG
HHHHHHHCCCCEEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
DEYVVLLVGIDSGGGLKVAERVRRAIEDHRFLSREGARVRVTASIGLASYPEHAQEKSEI
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHH
LDLADRAMYRGKRSSRNVVYMASKDLPPIPAGERP
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA