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The map label for this gene is livJ [C]

Identifier: 220916640

GI number: 220916640

Start: 1713330

End: 1714481

Strand: Reverse

Name: livJ [C]

Synonym: A2cp1_1534

Alternate gene names: 220916640

Gene position: 1714481-1713330 (Counterclockwise)

Preceding gene: 220916641

Following gene: 220916639

Centisome position: 34.09

GC content: 66.75

Gene sequence:

>1152_bases
ATGACGAAGAAGCTCGCGATGGCCGTGGCGGCGCTCGCCCTCGCGCTGCCCGGCACCGGCAGCGCGCAGGTGAAGATCGG
CTTCATCAACTCCATCACCGGCCCCGAGGCGCCCATCGGCGAGAACCTCACCAACGGCGTGGACATGGCCGTCGAGGATC
TCAAGAAGCAGGGCGTGAGCGTCACGATCGTGAAGCAGGACGACACCGGCAAGCCGCAGGTGGCCATGAGCGCCATGGAG
CAGCTCGCCACCGGCGACGAGGTCGCCGCCGTGGTCGGCCCGTACACCTCCGCCACCGCGAACGCCGTCGCGAAGCTGGC
CCAGCAGTACAAGGTCCCGCAGGTCGTTCCGGCCGCCGCGAAGGAGGAGATCACCAAGCAGGGCTACGACTGGGTGTTCC
GCCTGAACGCCCCGGCGCACCAGTACGCGCAGTCGCTCATCGACGCGGCGCTGACGTTCGGCAAGCCGAAGTCGATCGCG
TTCATCTACGAGTCCACCGACTTCGGCGCGTCGGTCGCCACCGCCGGCAAGGACTACGCGCAGAAGAAGGGCCTGAAGAT
CGTGGCGGACGAGGCCTACCAGAAGGGCGCGCCCGACTACCGCTCCACGCTCACCAAGATCAAGGCGCAGAACCCCGACC
TCGTCTTCATGGTCTCGTACGTCGCCGACGCCATCCTGCTGATGCGGCAGTCGCGCGAGGTGGGCCTGAAGCCGCAGGCG
TTCCTGGGCGGCGGCGCCGGCTTCGACACCGCGCAGTTCGAGAGCGAGAAGGACATCTCCAACCTGGTCTACTCGGTCAC
CCAGTGGACGCCCGAGTCGTCCAAGGCGGCCACCGCGTTCGCCGATCGCTACAAGGCGAAGTACGGCAAGAAGCCCACGT
ACCACGCGGCCTGCGCCTACGCGGCGATGATGATCGTGGGCGAGACCGCCGCGAAGAACGGCGGCGACCGGAAGAAGATC
CGAGACGCGCTCGCGAACGGCTCGTGGACCGGGATCATGGGCAAGGTGGACTTCGAGACGTACGAGGGCTTCACCAACCA
GAACAAGCTCGTCATGCCGGTGATCCAGTACCAGGGCGGCAAGTCGGAGACCGTCTTCCCTAAGGAAGCGGCGCAGAAGA
AGGCCGTCTACCCGTTCCCCGGCTGGAAGTAG

Upstream 100 bases:

>100_bases
CTCCTCGCAGCTGAAGTTCTAGCTTCTCCTCGAGGGGCGCCCGGGCGACGCCCGGGCGCCCTTCGTGTTTTCAGCGCACC
CCTTCACCCAGGAGCGAATC

Downstream 100 bases:

>100_bases
ATCGGCGCGCGCCGCGCGCGCACGCAGCGTGAAGCAGCTCGGAGCCGGGGGGCGGCGCGACCGCTCCCCGGCTCTCTTTT
TTCCGGCCCGGGCGGCCGTG

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTKKLAMAVAALALALPGTGSAQVKIGFINSITGPEAPIGENLTNGVDMAVEDLKKQGVSVTIVKQDDTGKPQVAMSAME
QLATGDEVAAVVGPYTSATANAVAKLAQQYKVPQVVPAAAKEEITKQGYDWVFRLNAPAHQYAQSLIDAALTFGKPKSIA
FIYESTDFGASVATAGKDYAQKKGLKIVADEAYQKGAPDYRSTLTKIKAQNPDLVFMVSYVADAILLMRQSREVGLKPQA
FLGGGAGFDTAQFESEKDISNLVYSVTQWTPESSKAATAFADRYKAKYGKKPTYHAACAYAAMMIVGETAAKNGGDRKKI
RDALANGSWTGIMGKVDFETYEGFTNQNKLVMPVIQYQGGKSETVFPKEAAQKKAVYPFPGWK

Sequences:

>Translated_383_residues
MTKKLAMAVAALALALPGTGSAQVKIGFINSITGPEAPIGENLTNGVDMAVEDLKKQGVSVTIVKQDDTGKPQVAMSAME
QLATGDEVAAVVGPYTSATANAVAKLAQQYKVPQVVPAAAKEEITKQGYDWVFRLNAPAHQYAQSLIDAALTFGKPKSIA
FIYESTDFGASVATAGKDYAQKKGLKIVADEAYQKGAPDYRSTLTKIKAQNPDLVFMVSYVADAILLMRQSREVGLKPQA
FLGGGAGFDTAQFESEKDISNLVYSVTQWTPESSKAATAFADRYKAKYGKKPTYHAACAYAAMMIVGETAAKNGGDRKKI
RDALANGSWTGIMGKVDFETYEGFTNQNKLVMPVIQYQGGKSETVFPKEAAQKKAVYPFPGWK
>Mature_382_residues
TKKLAMAVAALALALPGTGSAQVKIGFINSITGPEAPIGENLTNGVDMAVEDLKKQGVSVTIVKQDDTGKPQVAMSAMEQ
LATGDEVAAVVGPYTSATANAVAKLAQQYKVPQVVPAAAKEEITKQGYDWVFRLNAPAHQYAQSLIDAALTFGKPKSIAF
IYESTDFGASVATAGKDYAQKKGLKIVADEAYQKGAPDYRSTLTKIKAQNPDLVFMVSYVADAILLMRQSREVGLKPQAF
LGGGAGFDTAQFESEKDISNLVYSVTQWTPESSKAATAFADRYKAKYGKKPTYHAACAYAAMMIVGETAAKNGGDRKKIR
DALANGSWTGIMGKVDFETYEGFTNQNKLVMPVIQYQGGKSETVFPKEAAQKKAVYPFPGWK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=243, Percent_Identity=25.1028806584362, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789867, Length=237, Percent_Identity=24.4725738396624, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40991; Mature: 40860

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLAMAVAALALALPGTGSAQVKIGFINSITGPEAPIGENLTNGVDMAVEDLKKQGVS
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
VTIVKQDDTGKPQVAMSAMEQLATGDEVAAVVGPYTSATANAVAKLAQQYKVPQVVPAAA
EEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHH
KEEITKQGYDWVFRLNAPAHQYAQSLIDAALTFGKPKSIAFIYESTDFGASVATAGKDYA
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCHHHH
QKKGLKIVADEAYQKGAPDYRSTLTKIKAQNPDLVFMVSYVADAILLMRQSREVGLKPQA
HHCCCEEEECHHHHCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCC
FLGGGAGFDTAQFESEKDISNLVYSVTQWTPESSKAATAFADRYKAKYGKKPTYHAACAY
EECCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
AAMMIVGETAAKNGGDRKKIRDALANGSWTGIMGKVDFETYEGFTNQNKLVMPVIQYQGG
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCCEEEEEEEECCC
KSETVFPKEAAQKKAVYPFPGWK
CCCCCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TKKLAMAVAALALALPGTGSAQVKIGFINSITGPEAPIGENLTNGVDMAVEDLKKQGVS
CHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
VTIVKQDDTGKPQVAMSAMEQLATGDEVAAVVGPYTSATANAVAKLAQQYKVPQVVPAAA
EEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHH
KEEITKQGYDWVFRLNAPAHQYAQSLIDAALTFGKPKSIAFIYESTDFGASVATAGKDYA
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCHHHH
QKKGLKIVADEAYQKGAPDYRSTLTKIKAQNPDLVFMVSYVADAILLMRQSREVGLKPQA
HHCCCEEEECHHHHCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCC
FLGGGAGFDTAQFESEKDISNLVYSVTQWTPESSKAATAFADRYKAKYGKKPTYHAACAY
EECCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
AAMMIVGETAAKNGGDRKKIRDALANGSWTGIMGKVDFETYEGFTNQNKLVMPVIQYQGG
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCCEEEEEEEECCC
KSETVFPKEAAQKKAVYPFPGWK
CCCCCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA