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The map label for this gene is braE [H]

Identifier: 220916638

GI number: 220916638

Start: 1711140

End: 1712246

Strand: Reverse

Name: braE [H]

Synonym: A2cp1_1532

Alternate gene names: 220916638

Gene position: 1712246-1711140 (Counterclockwise)

Preceding gene: 220916639

Following gene: 220916637

Centisome position: 34.05

GC content: 68.2

Gene sequence:

>1107_bases
ATGCGCACCCGACTCCTCTCTCCCCTCCCCTCCCGCGCCCCGCGCGGCTTCTCGGTGGTGGGCCTGCTCGTGTTCCTGGC
GGTCGTCGCCGCGCTGGCGGTCTTCCCGCAGTTCGTGAAGAAGCCGTACGTGCTGCACATGGGCGTGCTGCTGTTCCTGG
CGGTGATCCAGGGCCAGGCCTGGAACGTGGTGGGCGGCTACGCCGGGCAGTACTCGGTCGGCCACGCCGCGTACTTCGGC
GTGGGCGCGTACGTCACCGTGATGCTGCTCGAGGTGAACAAGATCGCGCCGTGGTGGGGCGTGTGGGCGGCCATGGCGGC
GTCGGTGCTGCTGTCGCTCGTCATCGGCAGCATCACCTTCCGCCTGCGCGGGCCGTACTTCGTGCTCGCCTCGATCTCGG
TCGCCGAGATCATCCGGCTCGCCACCCTGTACTTCAAGGACCTCACCCGCGGCGCCGAGGGCTTCCTGCTCGGCGAGATC
CCGACGCTGAACCTGTTCGGCAACGAGATCATGTTCTTCGGCAAGCGGCCCTTCTACTTCGTGGGCCTGGGTCTCGCGGT
GGTGGCCGTCGCGGTCAACTGGATCGTCCAGCACTCCAAGCTCGGCTACTACTTCCAGGCCATCCGGGAGGACCAGGACG
CGGCGCACTCGCTCGGCATCAACCTGTCCTTCTACAAGAACGTGGCGCTCTCCATCTCGGCCGCCTTCACCGCGCTGGCC
GGCGGCTTCTACGCCATGTTCGTGAAGTTCATCGACCCGAACACGGTGTTCGGCCTGGACGTGTCGGTGCAGATCGTCCT
GCTCTGCATCATCGGCGGCATCGGGACCATCGTCGGCCCGGTGATCGGCGCGGTGGTGCTGGTGCCGCTCTCCGAGATCC
TGCGCAACCCGAAGGGTCTGGTGCAGGTCGGCGTGCTCTCGCCGGACTCGGGCTTCGTGACGTTCGTGGAGCGCTACCTC
TCCAACGCCCACCTGCTCGTGTACGGCATCCTGGTGGTGCTCGTGATCCTGTTCGCGCCCGAGGGCGTGCTCGGCGTCAT
CCGGCGCGTCACGGCGCGCTGGGGCCGCCGTCCGCAGGACGTCCCGCCCGCCGCGGCCAAGGCCTAG

Upstream 100 bases:

>100_bases
ATCGCCTCGGGGCTGAAGGAGCTGGTCACCTTCGTGCTGTTCCTCGCCCTGCTCCTCTTCAAGCCCGCCGGCCTGCTCGG
CAAGACGAGGGCCTGATCCG

Downstream 100 bases:

>100_bases
GAGAGACCGCCATGGCGATCCTCGAGATCAAGCACGTCTCCAAGTTCTTCGGCGGCCTCGCCGCGAACTCCGACGTCTCC
TTCACGATGGAGAAGGGGAT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MRTRLLSPLPSRAPRGFSVVGLLVFLAVVAALAVFPQFVKKPYVLHMGVLLFLAVIQGQAWNVVGGYAGQYSVGHAAYFG
VGAYVTVMLLEVNKIAPWWGVWAAMAASVLLSLVIGSITFRLRGPYFVLASISVAEIIRLATLYFKDLTRGAEGFLLGEI
PTLNLFGNEIMFFGKRPFYFVGLGLAVVAVAVNWIVQHSKLGYYFQAIREDQDAAHSLGINLSFYKNVALSISAAFTALA
GGFYAMFVKFIDPNTVFGLDVSVQIVLLCIIGGIGTIVGPVIGAVVLVPLSEILRNPKGLVQVGVLSPDSGFVTFVERYL
SNAHLLVYGILVVLVILFAPEGVLGVIRRVTARWGRRPQDVPPAAAKA

Sequences:

>Translated_368_residues
MRTRLLSPLPSRAPRGFSVVGLLVFLAVVAALAVFPQFVKKPYVLHMGVLLFLAVIQGQAWNVVGGYAGQYSVGHAAYFG
VGAYVTVMLLEVNKIAPWWGVWAAMAASVLLSLVIGSITFRLRGPYFVLASISVAEIIRLATLYFKDLTRGAEGFLLGEI
PTLNLFGNEIMFFGKRPFYFVGLGLAVVAVAVNWIVQHSKLGYYFQAIREDQDAAHSLGINLSFYKNVALSISAAFTALA
GGFYAMFVKFIDPNTVFGLDVSVQIVLLCIIGGIGTIVGPVIGAVVLVPLSEILRNPKGLVQVGVLSPDSGFVTFVERYL
SNAHLLVYGILVVLVILFAPEGVLGVIRRVTARWGRRPQDVPPAAAKA
>Mature_368_residues
MRTRLLSPLPSRAPRGFSVVGLLVFLAVVAALAVFPQFVKKPYVLHMGVLLFLAVIQGQAWNVVGGYAGQYSVGHAAYFG
VGAYVTVMLLEVNKIAPWWGVWAAMAASVLLSLVIGSITFRLRGPYFVLASISVAEIIRLATLYFKDLTRGAEGFLLGEI
PTLNLFGNEIMFFGKRPFYFVGLGLAVVAVAVNWIVQHSKLGYYFQAIREDQDAAHSLGINLSFYKNVALSISAAFTALA
GGFYAMFVKFIDPNTVFGLDVSVQIVLLCIIGGIGTIVGPVIGAVVLVPLSEILRNPKGLVQVGVLSPDSGFVTFVERYL
SNAHLLVYGILVVLVILFAPEGVLGVIRRVTARWGRRPQDVPPAAAKA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=326, Percent_Identity=25.1533742331288, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39661; Mature: 39661

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRLLSPLPSRAPRGFSVVGLLVFLAVVAALAVFPQFVKKPYVLHMGVLLFLAVIQGQA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
WNVVGGYAGQYSVGHAAYFGVGAYVTVMLLEVNKIAPWWGVWAAMAASVLLSLVIGSITF
CEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHE
RLRGPYFVLASISVAEIIRLATLYFKDLTRGAEGFLLGEIPTLNLFGNEIMFFGKRPFYF
EECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCEEEEECCCCHHH
VGLGLAVVAVAVNWIVQHSKLGYYFQAIREDQDAAHSLGINLSFYKNVALSISAAFTALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GGFYAMFVKFIDPNTVFGLDVSVQIVLLCIIGGIGTIVGPVIGAVVLVPLSEILRNPKGL
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VQVGVLSPDSGFVTFVERYLSNAHLLVYGILVVLVILFAPEGVLGVIRRVTARWGRRPQD
EEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
VPPAAAKA
CCCCCCCC
>Mature Secondary Structure
MRTRLLSPLPSRAPRGFSVVGLLVFLAVVAALAVFPQFVKKPYVLHMGVLLFLAVIQGQA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
WNVVGGYAGQYSVGHAAYFGVGAYVTVMLLEVNKIAPWWGVWAAMAASVLLSLVIGSITF
CEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHE
RLRGPYFVLASISVAEIIRLATLYFKDLTRGAEGFLLGEIPTLNLFGNEIMFFGKRPFYF
EECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCEEEEECCCCHHH
VGLGLAVVAVAVNWIVQHSKLGYYFQAIREDQDAAHSLGINLSFYKNVALSISAAFTALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GGFYAMFVKFIDPNTVFGLDVSVQIVLLCIIGGIGTIVGPVIGAVVLVPLSEILRNPKGL
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VQVGVLSPDSGFVTFVERYLSNAHLLVYGILVVLVILFAPEGVLGVIRRVTARWGRRPQD
EEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
VPPAAAKA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]