Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 220915878

GI number: 220915878

Start: 854164

End: 854721

Strand: Reverse

Name: def [H]

Synonym: A2cp1_0762

Alternate gene names: 220915878

Gene position: 854721-854164 (Counterclockwise)

Preceding gene: 220915879

Following gene: 220915865

Centisome position: 16.99

GC content: 69.71

Gene sequence:

>558_bases
ATGGTTCGTGAAATCGTCATCTGGCCCGACCCCATCCTGAAGGAAGTCGCCCATCCCGTGGACCGGGTGGACGACGGCAT
CCGCCGCCTCCTCGACGACATGGCCGAGACCATGTACGCCGCGGACGGCGTCGGCCTCGCCGCCCCCCAGATCGCGGTGA
AGAAGCGCGTCATCGTGATCGACACGTCCCCCCGCCAGGAGGGGCAGACGCTCATCCACCTGGTGAACCCGGAGATCGTC
CGCGGCGAGGGCGAGCTCACCTACACCGAGGGCTGCCTGTCGATCCCCGGCGAGGCCGAGGACGTGGACCGCTTCGCGCG
CGTGTGGGTGCGGGCGCTCGACTACCACGGCAAGCCGTTCGAGCTGGAGGCGGAGGGGCTGCTCGCGGTGGCGCTGCAGC
ACGAGACCGACCACCTGAACGGCACCGTGTTCGTGGACCACCTCTCCAGCCTCAAGCGCGAGCTCATCCGCAAGCGGATG
AAGAAGCTGAAGGCGCAGCGCGCCGCCGAGCCCGCCGCCGAGGTGAAGACCGCGGCGCGGCACGAGTCGGCGCTGTAG

Upstream 100 bases:

>100_bases
CCGCTGATCGGCCGGGCGAGCGCCTCCGGGCGCCCGATTCCGCGGGGATGCGCGGCGGCCCCTTCCCCGTTAGATGTGGG
ATCCGGTAGAAGGTGCTCGA

Downstream 100 bases:

>100_bases
CGCGTCCGGCGGCCCGCGCCGGAACGAGAGACGGCAGGCCCCGCGCGCGGGACCTGCCGTTCTCGCGTCTCGGGGGCGTG
GCCCTGGCTACAGCTCGCCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MVREIVIWPDPILKEVAHPVDRVDDGIRRLLDDMAETMYAADGVGLAAPQIAVKKRVIVIDTSPRQEGQTLIHLVNPEIV
RGEGELTYTEGCLSIPGEAEDVDRFARVWVRALDYHGKPFELEAEGLLAVALQHETDHLNGTVFVDHLSSLKRELIRKRM
KKLKAQRAAEPAAEVKTAARHESAL

Sequences:

>Translated_185_residues
MVREIVIWPDPILKEVAHPVDRVDDGIRRLLDDMAETMYAADGVGLAAPQIAVKKRVIVIDTSPRQEGQTLIHLVNPEIV
RGEGELTYTEGCLSIPGEAEDVDRFARVWVRALDYHGKPFELEAEGLLAVALQHETDHLNGTVFVDHLSSLKRELIRKRM
KKLKAQRAAEPAAEVKTAARHESAL
>Mature_185_residues
MVREIVIWPDPILKEVAHPVDRVDDGIRRLLDDMAETMYAADGVGLAAPQIAVKKRVIVIDTSPRQEGQTLIHLVNPEIV
RGEGELTYTEGCLSIPGEAEDVDRFARVWVRALDYHGKPFELEAEGLLAVALQHETDHLNGTVFVDHLSSLKRELIRKRM
KKLKAQRAAEPAAEVKTAARHESAL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=159, Percent_Identity=34.5911949685535, Blast_Score=84, Evalue=8e-17,
Organism=Escherichia coli, GI1789682, Length=167, Percent_Identity=48.502994011976, Blast_Score=159, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=31.8471337579618, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=32.484076433121, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20641; Mature: 20641

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVREIVIWPDPILKEVAHPVDRVDDGIRRLLDDMAETMYAADGVGLAAPQIAVKKRVIVI
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCEEEEE
DTSPRQEGQTLIHLVNPEIVRGEGELTYTEGCLSIPGEAEDVDRFARVWVRALDYHGKPF
ECCCCCCCCEEEEECCCEEEECCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHCCCCCC
ELEAEGLLAVALQHETDHLNGTVFVDHLSSLKRELIRKRMKKLKAQRAAEPAAEVKTAAR
EECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
HESAL
HHCCC
>Mature Secondary Structure
MVREIVIWPDPILKEVAHPVDRVDDGIRRLLDDMAETMYAADGVGLAAPQIAVKKRVIVI
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCEEEEE
DTSPRQEGQTLIHLVNPEIVRGEGELTYTEGCLSIPGEAEDVDRFARVWVRALDYHGKPF
ECCCCCCCCEEEEECCCEEEECCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHCCCCCC
ELEAEGLLAVALQHETDHLNGTVFVDHLSSLKRELIRKRMKKLKAQRAAEPAAEVKTAAR
EECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
HESAL
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA