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The map label for this gene is outF [H]

Identifier: 220915836

GI number: 220915836

Start: 807020

End: 808249

Strand: Reverse

Name: outF [H]

Synonym: A2cp1_0719

Alternate gene names: 220915836

Gene position: 808249-807020 (Counterclockwise)

Preceding gene: 220915837

Following gene: 220915835

Centisome position: 16.07

GC content: 70.16

Gene sequence:

>1230_bases
ATGCCGGTCTTCGAGTACAAGGCGTACGACGCCCAGGGCAAGGCGATCCAGGGCCTGCGCGAGGCGGACTCGCCGAAGGC
GCTGCGCAGCGTGCTCCGCCGCGACGGCGTGTTCCTCACCGAGGTGCTGGGCGAGCAGCAGGTGAAGGCCCAGCAGGCGC
GCGAGGTGAGCGTGCGCCGCTGGATGGTGGGCCGCGTCGGCCCGGTGAACCTCGCCATCGCCACCCGCCAGCTGTCGGTG
CTGGTGGGCGCGGGCATCCCGCTCGTCGAGGCGCTCACCGCGCTGGTGGACCAGGTCGAGCACGAGCGGCTGAAGCGCGT
GCTCGGCGACGTGAAGCAGCGCGTGAACGAGGGCGCGGCGCTGGCCGACGCGCTCGCCGCCCACCCGCGCGTGTTCTCGA
CGCTGTACGTGAACATGATCCGCGCCGGCGAGCACTCCGGCGCGCTCGAGGTGGTGCTGGTCCGGCTGGCCGACTTCACC
GAGAGCCAGGCCCGGCTGCGCTCGAAGATCATGGGCACGATGGCCTACCCGGCCGCGATGATGGTGATCGGCTCGGTCAT
CCTGGGGGTGCTGTTCACCGTGGTGATCCCGAAGATCACGAAGATCTTCGAGGACACGAAGGCGCAGCTCCCCTGGAACA
CCCGGGCGCTCATCGCCGCCTCGCACTTCGTCGCCGACTACTGGTGGCTCCTGCTCGCGCTCGTCGTGCTCTCGATCTGG
GGCTTCGTGCGGTGGCGGCGGACGCCGGCGGGGCGCGCCGCCTGGGATCGCGCGGCGCTGCGCTTCTGGATCTTCGGGCC
GCTGACGCGGCAGATCGCCATCAGCCGCTTCGCCCGCACGCTCTCCACGCTGCTCCGCAGCGGCGTCCCGCTGCTCACCG
CCATGGAGATCGTGAAGAACATCGTCGGCAACACGCGCCTCGCCGACGTGATCGACCAGGCCCGGGACGCGATCCGGGAG
GGCGAGTCCATCGCCGCCCCGCTGAAGCGCTCGGGCGAGTTCCCGCCGCTCGTCTACCACATGGTGGCGATCGGCGAGCG
CTCCGGCGCGCTCGAGGAGATGCTCGGCAACGTCGCCAAGGCGTACGAGGACCAGGTCGAGACGACGGTGAGCGCGCTCA
CCTCGCTGCTCGAGCCGCTCATGATCGTCGCCATGGGCGTGGTGGTCGCCTTCATCGTGTTCTCCGTCCTTCTTCCCATC
CTCCAGATCAACAACCTCGCAGGAGGCTAG

Upstream 100 bases:

>100_bases
TACAAGGTCGCGCGCGGCATCACCACCGCGGCCGAGGTGCTGTCGGTCACCGCGGAGGACATCCGGTGAGCGGCGCGCCG
CGCGCCCACGGAGCCCCCTG

Downstream 100 bases:

>100_bases
AGATGCACGCCCGCAACCGGCGCCGCGCACAGCGCGGCATGACCCTCATCGAGATCATGGTGGTGATCGTGATCCTCGGC
CTCATCGCCTCCGCGGTGGC

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Pectic enzymes secretion protein outF [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MPVFEYKAYDAQGKAIQGLREADSPKALRSVLRRDGVFLTEVLGEQQVKAQQAREVSVRRWMVGRVGPVNLAIATRQLSV
LVGAGIPLVEALTALVDQVEHERLKRVLGDVKQRVNEGAALADALAAHPRVFSTLYVNMIRAGEHSGALEVVLVRLADFT
ESQARLRSKIMGTMAYPAAMMVIGSVILGVLFTVVIPKITKIFEDTKAQLPWNTRALIAASHFVADYWWLLLALVVLSIW
GFVRWRRTPAGRAAWDRAALRFWIFGPLTRQIAISRFARTLSTLLRSGVPLLTAMEIVKNIVGNTRLADVIDQARDAIRE
GESIAAPLKRSGEFPPLVYHMVAIGERSGALEEMLGNVAKAYEDQVETTVSALTSLLEPLMIVAMGVVVAFIVFSVLLPI
LQINNLAGG

Sequences:

>Translated_409_residues
MPVFEYKAYDAQGKAIQGLREADSPKALRSVLRRDGVFLTEVLGEQQVKAQQAREVSVRRWMVGRVGPVNLAIATRQLSV
LVGAGIPLVEALTALVDQVEHERLKRVLGDVKQRVNEGAALADALAAHPRVFSTLYVNMIRAGEHSGALEVVLVRLADFT
ESQARLRSKIMGTMAYPAAMMVIGSVILGVLFTVVIPKITKIFEDTKAQLPWNTRALIAASHFVADYWWLLLALVVLSIW
GFVRWRRTPAGRAAWDRAALRFWIFGPLTRQIAISRFARTLSTLLRSGVPLLTAMEIVKNIVGNTRLADVIDQARDAIRE
GESIAAPLKRSGEFPPLVYHMVAIGERSGALEEMLGNVAKAYEDQVETTVSALTSLLEPLMIVAMGVVVAFIVFSVLLPI
LQINNLAGG
>Mature_408_residues
PVFEYKAYDAQGKAIQGLREADSPKALRSVLRRDGVFLTEVLGEQQVKAQQAREVSVRRWMVGRVGPVNLAIATRQLSVL
VGAGIPLVEALTALVDQVEHERLKRVLGDVKQRVNEGAALADALAAHPRVFSTLYVNMIRAGEHSGALEVVLVRLADFTE
SQARLRSKIMGTMAYPAAMMVIGSVILGVLFTVVIPKITKIFEDTKAQLPWNTRALIAASHFVADYWWLLLALVVLSIWG
FVRWRRTPAGRAAWDRAALRFWIFGPLTRQIAISRFARTLSTLLRSGVPLLTAMEIVKNIVGNTRLADVIDQARDAIREG
ESIAAPLKRSGEFPPLVYHMVAIGERSGALEEMLGNVAKAYEDQVETTVSALTSLLEPLMIVAMGVVVAFIVFSVLLPIL
QINNLAGG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=403, Percent_Identity=34.7394540942928, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1786295, Length=335, Percent_Identity=27.1641791044776, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44864; Mature: 44733

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PVFEYKAYDAQGKAIQGLREADSPKALRSVLRRDGVFLTEVLGEQQVKAQQAREVSVRR
CCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHH
WMVGRVGPVNLAIATRQLSVLVGAGIPLVEALTALVDQVEHERLKRVLGDVKQRVNEGAA
HHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LADALAAHPRVFSTLYVNMIRAGEHSGALEVVLVRLADFTESQARLRSKIMGTMAYPAAM
HHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MVIGSVILGVLFTVVIPKITKIFEDTKAQLPWNTRALIAASHFVADYWWLLLALVVLSIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GFVRWRRTPAGRAAWDRAALRFWIFGPLTRQIAISRFARTLSTLLRSGVPLLTAMEIVKN
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IVGNTRLADVIDQARDAIREGESIAAPLKRSGEFPPLVYHMVAIGERSGALEEMLGNVAK
HHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
AYEDQVETTVSALTSLLEPLMIVAMGVVVAFIVFSVLLPILQINNLAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]