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The map label for this gene is mexA [H]

Identifier: 220915666

GI number: 220915666

Start: 626973

End: 628205

Strand: Reverse

Name: mexA [H]

Synonym: A2cp1_0547

Alternate gene names: 220915666

Gene position: 628205-626973 (Counterclockwise)

Preceding gene: 220915669

Following gene: 220915665

Centisome position: 12.49

GC content: 75.26

Gene sequence:

>1233_bases
ATGAACCCCTCCCTCCGCTCCCCTCGCGCCGCCACGCGGCGCGCTCACGGCCTCCTTTTCGGCAAGCGCGCGCTCGCGCT
CGCCGCGCTCGCGCTCGTGGCCGCCGGCTGCGGCAAGAAGCCGGAGGCGCGCCCCCCCGGCCCGGTCGAGGTCGGCGTGA
TCACCGTCTCCCCGCAGGCGGTGACGCTCACCACCGAGCTCCCCGGCCGCGTCTCCGCGTTCAAGGTCGCCGAGGTCCGC
GCCCGCGTGAACGGCATCGTGCTGAAGCGCCTGTTCACCGAGGGCAGCGACGTGAAGCAGGGCCAGCCGCTGTTCAAGAT
CGATCCGGCGCCCTACCAGGCCGCGCTCGACAGCGCGCGGGCGCAGCTCGCCCGCGCCGACGCGACGCTCGCGTCGGCCA
AGCTCACCTCCGAGCGCTACACCGAGCTCGTCGCCGCGAACGCGGTGAGCCGGCAGGAGCACGAGGACGCCGTCGCCGCG
CTGAAGAAGGCCGAGGCCGACGTCGCCGCCGGCAAGGCCGCGGTGGACGCCGCCCGCATCAACCTCGGCTACACCACCGT
CACCTCGCCGGTGAGCGGCCGGATCGGCCGCTCGGGGGTCACCGAGGGCGCCTACGTCCAGGCCGCGCAGGCCACGCTGC
TCGCGACCGTGCAGCAGCTCGACCCGGTCTACGTGGACGTGACGCAGTCCAGCGCCGAGCTGCTCCGGATGCGGCGCGAT
CTCGAGGCCGGCAAGCTCCAGTCCGCCGGCGCCGGCAAGGCGAAGGTCGCGCTCACCACCGAGGATGGCCGCGAGTACGG
CCTCCCCGGCACGCTGCAGTTCGCCGACGTCACCGTGGATCCCGGCACTGGCTCGGTGGCGCTGCGCGCGATCTTCCCGA
ACCCGCGCGCCGAGCTGCTCCCCGGCACCTACGTGCGGGCGCGCCTGGCCGAGGGCGTGAACCCGGCGGCGCTTCTCGTC
CCCCAGCGCGCCGTCGCCCGCAACAACAAGGGCGAGCCCACGGCGCTGATCGTCGCCGGCGGCAAGGTCGAGCTGCGCAA
CCTGGTCACCGAGCGCGTCATCGGCGACGCGTGGCTCGTGACCTCCGGCGTGAAGCCCGGCGAGCAGGTGATCGTCGAGG
GCCTCCAGAAGGTGCGCCCGGGCGCCCCCGTCAACCCGGTCCCGGCCGGCGGCGCGAAGCAGGCGAACGCCGCGACCCCG
GGCCAGCAGGCCCCGGCGGGCGCGGCCCGCTGA

Upstream 100 bases:

>100_bases
TGAACTGAACGGTTTAGTGTTGACCCCGTCCGGCCGCATGGCTATCAGTACCGCCCTCCCGGGCTCACGGAACCGTTCCC
CGGGCCTCAAAGGAATCGAG

Downstream 100 bases:

>100_bases
GGCGCCCGACAGCGTGACCGCGCGCGCGGCCGGACCCGAGCCGGCCGCCCGCGCACCCCTTCCCTTTTTTCAGTCGAGGA
GTCGTCGTGGCCAGGTTCTT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNPSLRSPRAATRRAHGLLFGKRALALAALALVAAGCGKKPEARPPGPVEVGVITVSPQAVTLTTELPGRVSAFKVAEVR
ARVNGIVLKRLFTEGSDVKQGQPLFKIDPAPYQAALDSARAQLARADATLASAKLTSERYTELVAANAVSRQEHEDAVAA
LKKAEADVAAGKAAVDAARINLGYTTVTSPVSGRIGRSGVTEGAYVQAAQATLLATVQQLDPVYVDVTQSSAELLRMRRD
LEAGKLQSAGAGKAKVALTTEDGREYGLPGTLQFADVTVDPGTGSVALRAIFPNPRAELLPGTYVRARLAEGVNPAALLV
PQRAVARNNKGEPTALIVAGGKVELRNLVTERVIGDAWLVTSGVKPGEQVIVEGLQKVRPGAPVNPVPAGGAKQANAATP
GQQAPAGAAR

Sequences:

>Translated_410_residues
MNPSLRSPRAATRRAHGLLFGKRALALAALALVAAGCGKKPEARPPGPVEVGVITVSPQAVTLTTELPGRVSAFKVAEVR
ARVNGIVLKRLFTEGSDVKQGQPLFKIDPAPYQAALDSARAQLARADATLASAKLTSERYTELVAANAVSRQEHEDAVAA
LKKAEADVAAGKAAVDAARINLGYTTVTSPVSGRIGRSGVTEGAYVQAAQATLLATVQQLDPVYVDVTQSSAELLRMRRD
LEAGKLQSAGAGKAKVALTTEDGREYGLPGTLQFADVTVDPGTGSVALRAIFPNPRAELLPGTYVRARLAEGVNPAALLV
PQRAVARNNKGEPTALIVAGGKVELRNLVTERVIGDAWLVTSGVKPGEQVIVEGLQKVRPGAPVNPVPAGGAKQANAATP
GQQAPAGAAR
>Mature_410_residues
MNPSLRSPRAATRRAHGLLFGKRALALAALALVAAGCGKKPEARPPGPVEVGVITVSPQAVTLTTELPGRVSAFKVAEVR
ARVNGIVLKRLFTEGSDVKQGQPLFKIDPAPYQAALDSARAQLARADATLASAKLTSERYTELVAANAVSRQEHEDAVAA
LKKAEADVAAGKAAVDAARINLGYTTVTSPVSGRIGRSGVTEGAYVQAAQATLLATVQQLDPVYVDVTQSSAELLRMRRD
LEAGKLQSAGAGKAKVALTTEDGREYGLPGTLQFADVTVDPGTGSVALRAIFPNPRAELLPGTYVRARLAEGVNPAALLV
PQRAVARNNKGEPTALIVAGGKVELRNLVTERVIGDAWLVTSGVKPGEQVIVEGLQKVRPGAPVNPVPAGGAKQANAATP
GQQAPAGAAR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=381, Percent_Identity=53.8057742782152, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=52.0215633423181, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1789929, Length=355, Percent_Identity=48.4507042253521, Blast_Score=327, Evalue=8e-91,
Organism=Escherichia coli, GI87082036, Length=345, Percent_Identity=29.5652173913043, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081796, Length=185, Percent_Identity=29.1891891891892, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42380; Mature: 42380

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSLRSPRAATRRAHGLLFGKRALALAALALVAAGCGKKPEARPPGPVEVGVITVSPQA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCE
VTLTTELPGRVSAFKVAEVRARVNGIVLKRLFTEGSDVKQGQPLFKIDPAPYQAALDSAR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHH
AQLARADATLASAKLTSERYTELVAANAVSRQEHEDAVAALKKAEADVAAGKAAVDAARI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEE
NLGYTTVTSPVSGRIGRSGVTEGAYVQAAQATLLATVQQLDPVYVDVTQSSAELLRMRRD
ECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
LEAGKLQSAGAGKAKVALTTEDGREYGLPGTLQFADVTVDPGTGSVALRAIFPNPRAELL
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHCC
PGTYVRARLAEGVNPAALLVPQRAVARNNKGEPTALIVAGGKVELRNLVTERVIGDAWLV
CCHHHHHHHHCCCCCCEEEECCHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEE
TSGVKPGEQVIVEGLQKVRPGAPVNPVPAGGAKQANAATPGQQAPAGAAR
ECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPSLRSPRAATRRAHGLLFGKRALALAALALVAAGCGKKPEARPPGPVEVGVITVSPQA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCE
VTLTTELPGRVSAFKVAEVRARVNGIVLKRLFTEGSDVKQGQPLFKIDPAPYQAALDSAR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHH
AQLARADATLASAKLTSERYTELVAANAVSRQEHEDAVAALKKAEADVAAGKAAVDAARI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEE
NLGYTTVTSPVSGRIGRSGVTEGAYVQAAQATLLATVQQLDPVYVDVTQSSAELLRMRRD
ECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
LEAGKLQSAGAGKAKVALTTEDGREYGLPGTLQFADVTVDPGTGSVALRAIFPNPRAELL
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHCC
PGTYVRARLAEGVNPAALLVPQRAVARNNKGEPTALIVAGGKVELRNLVTERVIGDAWLV
CCHHHHHHHHCCCCCCEEEECCHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEE
TSGVKPGEQVIVEGLQKVRPGAPVNPVPAGGAKQANAATPGQQAPAGAAR
ECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]