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The map label for this gene is oprM [H]

Identifier: 220915664

GI number: 220915664

Start: 622311

End: 623723

Strand: Reverse

Name: oprM [H]

Synonym: A2cp1_0545

Alternate gene names: 220915664

Gene position: 623723-622311 (Counterclockwise)

Preceding gene: 220915665

Following gene: 220915662

Centisome position: 12.4

GC content: 73.18

Gene sequence:

>1413_bases
ATGCGTAGCCCTGGCCTCGCGACGCTCGTCGCCGCCTCCGCCCTCGCCGTCTCCGGGTGCACGCTCGCGCCCCGGTACCA
GCGCCCCGCCGCGCCCGTGGCCGCCGAGTTCCCCGCCGGCGCCGGCGCGGCGACCGGCCCCGCCGCTTCCGACCTGGGCT
GGCGCGACGTGTTCCAGGATCCCGGCCTCCAGGCCGTGATCGACCAGGCGCTCCGCGAGAACCGCGACCTCCGCGTCGCC
GCGCTCAACGTGGAGCTGGCCCGTGCGCAGTACGGCATCCAGCGCTCGTACCTGCTGCCCACGGTCGGCGCGACCGGGAG
CGGCAGCCGCGCGCGCACGGCGAAGGACCTCAGCTTCACCGGCGAGCCCTCCGTCGGGAACACCTTCTCGGCCGGCGTGG
GCCTCACGTCGTTCGAGATCGACCTGTTCGGCCGCGTCCGCAGCCTGCGGAACCAGGCGCTCGAGCAGTACCTCTCGACC
GAGGAGGCCCACCGCAGCGCGCACCTGGCGCTCGTCTCCGAGGTGGCGGTCCAGTACCTCGCCTCGCGCGCGCTGCAGTC
GCAGGTGGCGCTCGCGGAGCAGACGCTGCAGACCGTGCAGCAGTCGTACGACCTCGCGAAGCGCACCTTCGAGGCCGGCC
GCACCTCCGAGCTCGACTTCCGCACCGCCGAGGCGCAGGTCGAGACCGCGCGGGTGAACCTGTCCGCCTACCAGCAGCAG
CTCGCGCAGGCCGAGAACGCGCTGGTGCTGCTCGCCGGCGGACCGCTGCCGAAGGACCTGCCGACGGCGCCGCTCGAGTC
GCAGCCTCCCGTGGCGGAGCTGCCGGCGGGGCTGCCGTCCGACCTGCTGCAGCGCCGTCCCGACATCCTCTCGGCCGAGC
ATCAGCTCAAGGCGGCGAACGCCAGCATCGGCGCGGCGCGCGCGGCGTTCTTCCCCTCCATCTCGCTCACCGCCTCGGTG
GGCACCGCCTCGACCGAGCTGTCCAACCTGTTCACCGCCGGCTCGGGCACCTGGGCGTTCTCGCCGCGCATCGACCTGCC
CATCTTCACCGGCGGCCGCAACAAGGCGAACCTCGACGCGGCGAACGTCCGCAAGCAGATCGAGATCGCGAGCTACGAGA
AGGCGATCCAGGTGGCGTTCCGCGAGGTGGCCGACGCGCTGGTGGCGCGCGGGCGCCTCGACGAGCAGCTCGAGGCCCAG
ACCCGGCGCGTCCAGGCGGAGCAGCGGCGCTACGACATCTCCGAGCTGCGCTACCGCAAGGGCGTGGACAGCTACCTGAA
CGTGCTCACCGCGCAGCGCGACCTGTACGGCGCGCAGCAGCAGCTCATCCAGACCCGGCTCACCCGCTTCTCGAACTCCA
TCGGCCTGTACCGCGCCCTCGGCGGCGGATGGCTGGAGCACGGGAAGTCCTGA

Upstream 100 bases:

>100_bases
TTCTTCGTGAAGATCGCCGGCAAGCGGAAGACGCCGCCGCCCGACCTGGTGGCCGGACCGCGCGACGACGAGGACGAGGC
CGCGCCGAAGGAGATCGCCC

Downstream 100 bases:

>100_bases
CGGGGCTGCGCCCCGCCCCACGGAGCAGAGCTCCGCGGGGCCCCACCCTGCTCGCCGGGTCCCCTGCGCCGCCGCGGGCG
ACTTCGCCGCTGACGCGGCG

Product: NodT family RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRSPGLATLVAASALAVSGCTLAPRYQRPAAPVAAEFPAGAGAATGPAASDLGWRDVFQDPGLQAVIDQALRENRDLRVA
ALNVELARAQYGIQRSYLLPTVGATGSGSRARTAKDLSFTGEPSVGNTFSAGVGLTSFEIDLFGRVRSLRNQALEQYLST
EEAHRSAHLALVSEVAVQYLASRALQSQVALAEQTLQTVQQSYDLAKRTFEAGRTSELDFRTAEAQVETARVNLSAYQQQ
LAQAENALVLLAGGPLPKDLPTAPLESQPPVAELPAGLPSDLLQRRPDILSAEHQLKAANASIGAARAAFFPSISLTASV
GTASTELSNLFTAGSGTWAFSPRIDLPIFTGGRNKANLDAANVRKQIEIASYEKAIQVAFREVADALVARGRLDEQLEAQ
TRRVQAEQRRYDISELRYRKGVDSYLNVLTAQRDLYGAQQQLIQTRLTRFSNSIGLYRALGGGWLEHGKS

Sequences:

>Translated_470_residues
MRSPGLATLVAASALAVSGCTLAPRYQRPAAPVAAEFPAGAGAATGPAASDLGWRDVFQDPGLQAVIDQALRENRDLRVA
ALNVELARAQYGIQRSYLLPTVGATGSGSRARTAKDLSFTGEPSVGNTFSAGVGLTSFEIDLFGRVRSLRNQALEQYLST
EEAHRSAHLALVSEVAVQYLASRALQSQVALAEQTLQTVQQSYDLAKRTFEAGRTSELDFRTAEAQVETARVNLSAYQQQ
LAQAENALVLLAGGPLPKDLPTAPLESQPPVAELPAGLPSDLLQRRPDILSAEHQLKAANASIGAARAAFFPSISLTASV
GTASTELSNLFTAGSGTWAFSPRIDLPIFTGGRNKANLDAANVRKQIEIASYEKAIQVAFREVADALVARGRLDEQLEAQ
TRRVQAEQRRYDISELRYRKGVDSYLNVLTAQRDLYGAQQQLIQTRLTRFSNSIGLYRALGGGWLEHGKS
>Mature_470_residues
MRSPGLATLVAASALAVSGCTLAPRYQRPAAPVAAEFPAGAGAATGPAASDLGWRDVFQDPGLQAVIDQALRENRDLRVA
ALNVELARAQYGIQRSYLLPTVGATGSGSRARTAKDLSFTGEPSVGNTFSAGVGLTSFEIDLFGRVRSLRNQALEQYLST
EEAHRSAHLALVSEVAVQYLASRALQSQVALAEQTLQTVQQSYDLAKRTFEAGRTSELDFRTAEAQVETARVNLSAYQQQ
LAQAENALVLLAGGPLPKDLPTAPLESQPPVAELPAGLPSDLLQRRPDILSAEHQLKAANASIGAARAAFFPSISLTASV
GTASTELSNLFTAGSGTWAFSPRIDLPIFTGGRNKANLDAANVRKQIEIASYEKAIQVAFREVADALVARGRLDEQLEAQ
TRRVQAEQRRYDISELRYRKGVDSYLNVLTAQRDLYGAQQQLIQTRLTRFSNSIGLYRALGGGWLEHGKS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=456, Percent_Identity=43.859649122807, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI2367348, Length=476, Percent_Identity=26.4705882352941, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50447; Mature: 50447

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPGLATLVAASALAVSGCTLAPRYQRPAAPVAAEFPAGAGAATGPAASDLGWRDVFQD
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC
PGLQAVIDQALRENRDLRVAALNVELARAQYGIQRSYLLPTVGATGSGSRARTAKDLSFT
CCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
GEPSVGNTFSAGVGLTSFEIDLFGRVRSLRNQALEQYLSTEEAHRSAHLALVSEVAVQYL
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRALQSQVALAEQTLQTVQQSYDLAKRTFEAGRTSELDFRTAEAQVETARVNLSAYQQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
LAQAENALVLLAGGPLPKDLPTAPLESQPPVAELPAGLPSDLLQRRPDILSAEHQLKAAN
HHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHCCCCHHCHHHHHHHHC
ASIGAARAAFFPSISLTASVGTASTELSNLFTAGSGTWAFSPRIDLPIFTGGRNKANLDA
CCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCEEECCCCCCEEEECCCCCCCCCH
ANVRKQIEIASYEKAIQVAFREVADALVARGRLDEQLEAQTRRVQAEQRRYDISELRYRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
GVDSYLNVLTAQRDLYGAQQQLIQTRLTRFSNSIGLYRALGGGWLEHGKS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MRSPGLATLVAASALAVSGCTLAPRYQRPAAPVAAEFPAGAGAATGPAASDLGWRDVFQD
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC
PGLQAVIDQALRENRDLRVAALNVELARAQYGIQRSYLLPTVGATGSGSRARTAKDLSFT
CCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
GEPSVGNTFSAGVGLTSFEIDLFGRVRSLRNQALEQYLSTEEAHRSAHLALVSEVAVQYL
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRALQSQVALAEQTLQTVQQSYDLAKRTFEAGRTSELDFRTAEAQVETARVNLSAYQQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
LAQAENALVLLAGGPLPKDLPTAPLESQPPVAELPAGLPSDLLQRRPDILSAEHQLKAAN
HHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHCCCCHHCHHHHHHHHC
ASIGAARAAFFPSISLTASVGTASTELSNLFTAGSGTWAFSPRIDLPIFTGGRNKANLDA
CCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCEEECCCCCCEEEECCCCCCCCCH
ANVRKQIEIASYEKAIQVAFREVADALVARGRLDEQLEAQTRRVQAEQRRYDISELRYRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
GVDSYLNVLTAQRDLYGAQQQLIQTRLTRFSNSIGLYRALGGGWLEHGKS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]