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The map label for this gene is hypF2 [H]

Identifier: 220915623

GI number: 220915623

Start: 578115

End: 580436

Strand: Reverse

Name: hypF2 [H]

Synonym: A2cp1_0504

Alternate gene names: 220915623

Gene position: 580436-578115 (Counterclockwise)

Preceding gene: 220915624

Following gene: 220915622

Centisome position: 11.54

GC content: 78.17

Gene sequence:

>2322_bases
ATGACCACGCAGCGGACCACAGACGGCGGGCCGGACCTCTCCGCCGAAGGGCGACGCATCGCCGTGAGCGGCACGGTGCA
GGGCGTCGGCTTCCGTCCGTTCGTCTACCGGCTCGCGCACCAGACCGGCGTGACCGGCCGCGTCCGGAACGACGCCGCCG
GCGTGACCATCGAGGCGTTCGGCTCGCCCGCCGCGCTCGACGCGTTCCAGGCCCGCCTGGTGAGCGACCGCCCGCCCGCA
GCCTCGATCGCAGCGCTGCGCGCCGAGGTGATCCCCGCCGAGCCGGCGCCGCGCTTCGAGATCGTCGAGAGCGGCAGGTC
GGCCGAGCGCCGGGTCGCCATCCCGCCGGATCTCGCCACCTGCCCCGAGTGCCTGCGCGAGCTCGGCGACCCCGCCGATC
GCCGCCACCGCTACCCGTTCACGAACTGCACCGCCTGCGGCCCGCGCTTCACCATCGCGCTCGACGTCCCTTACGACCGG
CCCGCCACCACCATGGCCGGCTTCCGGATGTGCCCCGCCTGCGCGCGCGAGTACGGCGACCCGCTCGACCGGCGCTTCCA
CGCGCAGCCGAACGCCTGCCCCGCCTGCGGCCCGCGCGTCGCGCTGCGCGACGCCGCCGGCGCGCCACGACCCTCGGCCG
ACCCGATCCGCGACTGCGCCGACCTGCTGCGGGCGGGCGGGATCGCCGCGGTGAAGGGGCTCGGCGGGTACCACCTCGCG
TGCGACGCGAGCGCGCCCGCCGCGGTCCGGACGCTGCGCGAGCGGAAGCGGCGCGAGGAGAAGCCGCTCGCGGTGATGGT
GGCCGACCTCGACGCCGCGCGGGCGCTCGCCGAGGTCTCGCCGGCGGAGGCGGCGCTGCTCACGTCTCCCGAGCGCCCCA
TCGTGCTGTGCCGGCGGCGCCCGGACGCGGCGCTCGCCGCCGAGATCGCGCCGGGCAGCCCGATCGTCGGCCTCATGCTC
GCGTACGCGCCGCTCCACCATCTCCTGCTCGCCGACGCGGGCGTGCCGCTGGTGATGACGAGCGCGAACCTCTCCGACGA
GCCCATCGCCTTCGAGGACGGCGAGGCGCTGGCGCGGCTCGCCGGCATCGCCGACCTGTTCCTGGTGCACGACCGGGCCA
TCGCGTCGCGCTGCGACGACTCGGTGGCGCGGGTGGTGGCGGGGCGGCCCATGGTCATGCGCCGCAGCCGCGGGTTCGTG
CCGCGCCCGGTGCGCACCGCCCGGCGCTTCGCCCGCCCGGTGCTGGCGGCGGGCGCGCAGCTCAAGAACGCCTGCTGCCT
GGCGCGCGGGGACGAGGCCACCCTCGGCCCGCACGTGGGAGACCTCGACGGCCTCGAGACCTACGGCGCGTTCGAGGCGG
CGGTGGCGCGGCTGGAGACGTTCCTCGCCTTCCGCCCCGAGCTGCTCGCCTGCGACCTCCACCCGCTCTACCTCTCCACG
CGCTGGGCCCGCGAGCGCGCCGGGGCGCTGGCGCTCCCGCTCGTCGAGGTCCAGCACCACCACGCGCACGCGGCCGCCTG
CATGGCCGAGCACGGGCTCGAGGGCCCGGCGCTGGCGCTGTGCTGGGACGGCACCGGGCTCGGGACCGACGGCGCGTCCT
GGGGCGGGGAGCTGCTGCTCGCCGAGGCGGCGCGGTTCGACCGGCTCGCGACCTTCCGGCCGGTGCCACTCGCGGGCGGC
GACCGCGCCATCCGCGATCCCTGGCGCATCGCGCTCGCCGCGCTGCTCGACGCGTTCGGCGAGGACGCGCCGCTCGACCG
GCTCCCCCTGTTCGCGGGGGTGCCGGCGCGGGAGCTGGAGGTCGTCCGCCGCATGCTGGCCACCGGCCTGAACGCGCCGC
CCGCGCACGGCGCCGGCCGCGCCTTCGACGCCGCGGGCGCGCTGGCGCTCTGCCGCCCTTCGGCGCGCTTCGAGGGCCAG
GTGGCGCTCGCGCTCGACGCGGCCGCGCACGGCCACGAGGCGGCGCCCTACCCGTTCGACCTCGACGCGTCCGGGCCGGT
GGAGGCGCTCGACCTGCGCCCGCTCTGGCGTGCGCTCGCCGCGGACGTCCTCGCGGCCCGGCCGGCGGGGGAGATGTCGG
CCCGGTTCCACGCGGCGCTCGCCGCGGCCGGCGCCGAGCAGGTCCGGCGCGCGGCGCGCCGGACGGGGAAGCTGCCGGTG
GTGCTGACCGGCGGCTGCTTCCAGAACGCGCGGCTCGCGGAGGCGATCCGCGCGCGGCTGGACGGGGAGTTCGCGGTATA
CCAGCACGGCGAGGTGCCGCCCGGCGACGGCGGGATCGCGCTCGGCCAGGCGGTGGTCGCCGACGCGGTGGCCCGCGCCT
AG

Upstream 100 bases:

>100_bases
TGATCGACCGGGTGGCCGCCGCGCGTTGCGGCGGCGAAACGCGCGGCGGTAAGCTCGCGCCGCCCCAGCACGGAAGGCCG
AGGCGCACGCGCCGCGCCGC

Downstream 100 bases:

>100_bases
CGCCGGCCGCTGGAGGAGACGGAAGACATGTGCCTCGGGGTTCCAGGGAAGGTCATCGCCATCGACGGGCTCGACGCCAC
GGTGGACTTCTTCGGCGTGC

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF2; [NiFe]-hydrogenase maturation factor hypF2; Hydrogenase maturation protein hypF2 [H]

Number of amino acids: Translated: 773; Mature: 772

Protein sequence:

>773_residues
MTTQRTTDGGPDLSAEGRRIAVSGTVQGVGFRPFVYRLAHQTGVTGRVRNDAAGVTIEAFGSPAALDAFQARLVSDRPPA
ASIAALRAEVIPAEPAPRFEIVESGRSAERRVAIPPDLATCPECLRELGDPADRRHRYPFTNCTACGPRFTIALDVPYDR
PATTMAGFRMCPACAREYGDPLDRRFHAQPNACPACGPRVALRDAAGAPRPSADPIRDCADLLRAGGIAAVKGLGGYHLA
CDASAPAAVRTLRERKRREEKPLAVMVADLDAARALAEVSPAEAALLTSPERPIVLCRRRPDAALAAEIAPGSPIVGLML
AYAPLHHLLLADAGVPLVMTSANLSDEPIAFEDGEALARLAGIADLFLVHDRAIASRCDDSVARVVAGRPMVMRRSRGFV
PRPVRTARRFARPVLAAGAQLKNACCLARGDEATLGPHVGDLDGLETYGAFEAAVARLETFLAFRPELLACDLHPLYLST
RWARERAGALALPLVEVQHHHAHAAACMAEHGLEGPALALCWDGTGLGTDGASWGGELLLAEAARFDRLATFRPVPLAGG
DRAIRDPWRIALAALLDAFGEDAPLDRLPLFAGVPARELEVVRRMLATGLNAPPAHGAGRAFDAAGALALCRPSARFEGQ
VALALDAAAHGHEAAPYPFDLDASGPVEALDLRPLWRALAADVLAARPAGEMSARFHAALAAAGAEQVRRAARRTGKLPV
VLTGGCFQNARLAEAIRARLDGEFAVYQHGEVPPGDGGIALGQAVVADAVARA

Sequences:

>Translated_773_residues
MTTQRTTDGGPDLSAEGRRIAVSGTVQGVGFRPFVYRLAHQTGVTGRVRNDAAGVTIEAFGSPAALDAFQARLVSDRPPA
ASIAALRAEVIPAEPAPRFEIVESGRSAERRVAIPPDLATCPECLRELGDPADRRHRYPFTNCTACGPRFTIALDVPYDR
PATTMAGFRMCPACAREYGDPLDRRFHAQPNACPACGPRVALRDAAGAPRPSADPIRDCADLLRAGGIAAVKGLGGYHLA
CDASAPAAVRTLRERKRREEKPLAVMVADLDAARALAEVSPAEAALLTSPERPIVLCRRRPDAALAAEIAPGSPIVGLML
AYAPLHHLLLADAGVPLVMTSANLSDEPIAFEDGEALARLAGIADLFLVHDRAIASRCDDSVARVVAGRPMVMRRSRGFV
PRPVRTARRFARPVLAAGAQLKNACCLARGDEATLGPHVGDLDGLETYGAFEAAVARLETFLAFRPELLACDLHPLYLST
RWARERAGALALPLVEVQHHHAHAAACMAEHGLEGPALALCWDGTGLGTDGASWGGELLLAEAARFDRLATFRPVPLAGG
DRAIRDPWRIALAALLDAFGEDAPLDRLPLFAGVPARELEVVRRMLATGLNAPPAHGAGRAFDAAGALALCRPSARFEGQ
VALALDAAAHGHEAAPYPFDLDASGPVEALDLRPLWRALAADVLAARPAGEMSARFHAALAAAGAEQVRRAARRTGKLPV
VLTGGCFQNARLAEAIRARLDGEFAVYQHGEVPPGDGGIALGQAVVADAVARA
>Mature_772_residues
TTQRTTDGGPDLSAEGRRIAVSGTVQGVGFRPFVYRLAHQTGVTGRVRNDAAGVTIEAFGSPAALDAFQARLVSDRPPAA
SIAALRAEVIPAEPAPRFEIVESGRSAERRVAIPPDLATCPECLRELGDPADRRHRYPFTNCTACGPRFTIALDVPYDRP
ATTMAGFRMCPACAREYGDPLDRRFHAQPNACPACGPRVALRDAAGAPRPSADPIRDCADLLRAGGIAAVKGLGGYHLAC
DASAPAAVRTLRERKRREEKPLAVMVADLDAARALAEVSPAEAALLTSPERPIVLCRRRPDAALAAEIAPGSPIVGLMLA
YAPLHHLLLADAGVPLVMTSANLSDEPIAFEDGEALARLAGIADLFLVHDRAIASRCDDSVARVVAGRPMVMRRSRGFVP
RPVRTARRFARPVLAAGAQLKNACCLARGDEATLGPHVGDLDGLETYGAFEAAVARLETFLAFRPELLACDLHPLYLSTR
WARERAGALALPLVEVQHHHAHAAACMAEHGLEGPALALCWDGTGLGTDGASWGGELLLAEAARFDRLATFRPVPLAGGD
RAIRDPWRIALAALLDAFGEDAPLDRLPLFAGVPARELEVVRRMLATGLNAPPAHGAGRAFDAAGALALCRPSARFEGQV
ALALDAAAHGHEAAPYPFDLDASGPVEALDLRPLWRALAADVLAARPAGEMSARFHAALAAAGAEQVRRAARRTGKLPVV
LTGGCFQNARLAEAIRARLDGEFAVYQHGEVPPGDGGIALGQAVVADAVARA

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=765, Percent_Identity=41.6993464052288, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 81455; Mature: 81324

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQRTTDGGPDLSAEGRRIAVSGTVQGVGFRPFVYRLAHQTGVTGRVRNDAAGVTIEAF
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEEC
GSPAALDAFQARLVSDRPPAASIAALRAEVIPAEPAPRFEIVESGRSAERRVAIPPDLAT
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEECCCCHHH
CPECLRELGDPADRRHRYPFTNCTACGPRFTIALDVPYDRPATTMAGFRMCPACAREYGD
HHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCHHHHHHHCC
PLDRRFHAQPNACPACGPRVALRDAAGAPRPSADPIRDCADLLRAGGIAAVKGLGGYHLA
HHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCHHEECCCCCEEEE
CDASAPAAVRTLRERKRREEKPLAVMVADLDAARALAEVSPAEAALLTSPERPIVLCRRR
ECCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCHHHHEECCCCCCEEEEECC
PDAALAAEIAPGSPIVGLMLAYAPLHHLLLADAGVPLVMTSANLSDEPIAFEDGEALARL
CCHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCHHHHHHH
AGIADLFLVHDRAIASRCDDSVARVVAGRPMVMRRSRGFVPRPVRTARRFARPVLAAGAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCCCHHHHHHHHHHHHHHCHH
LKNACCLARGDEATLGPHVGDLDGLETYGAFEAAVARLETFLAFRPELLACDLHPLYLST
HHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHH
RWARERAGALALPLVEVQHHHAHAAACMAEHGLEGPALALCWDGTGLGTDGASWGGELLL
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHH
AEAARFDRLATFRPVPLAGGDRAIRDPWRIALAALLDAFGEDAPLDRLPLFAGVPARELE
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHH
VVRRMLATGLNAPPAHGAGRAFDAAGALALCRPSARFEGQVALALDAAAHGHEAAPYPFD
HHHHHHHCCCCCCCCCCCCCCHHHCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
LDASGPVEALDLRPLWRALAADVLAARPAGEMSARFHAALAAAGAEQVRRAARRTGKLPV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCE
VLTGGCFQNARLAEAIRARLDGEFAVYQHGEVPPGDGGIALGQAVVADAVARA
EEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQRTTDGGPDLSAEGRRIAVSGTVQGVGFRPFVYRLAHQTGVTGRVRNDAAGVTIEAF
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEEC
GSPAALDAFQARLVSDRPPAASIAALRAEVIPAEPAPRFEIVESGRSAERRVAIPPDLAT
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEECCCCHHH
CPECLRELGDPADRRHRYPFTNCTACGPRFTIALDVPYDRPATTMAGFRMCPACAREYGD
HHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCHHHHHHHCC
PLDRRFHAQPNACPACGPRVALRDAAGAPRPSADPIRDCADLLRAGGIAAVKGLGGYHLA
HHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCHHEECCCCCEEEE
CDASAPAAVRTLRERKRREEKPLAVMVADLDAARALAEVSPAEAALLTSPERPIVLCRRR
ECCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCHHHHEECCCCCCEEEEECC
PDAALAAEIAPGSPIVGLMLAYAPLHHLLLADAGVPLVMTSANLSDEPIAFEDGEALARL
CCHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCHHHHHHH
AGIADLFLVHDRAIASRCDDSVARVVAGRPMVMRRSRGFVPRPVRTARRFARPVLAAGAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCCCHHHHHHHHHHHHHHCHH
LKNACCLARGDEATLGPHVGDLDGLETYGAFEAAVARLETFLAFRPELLACDLHPLYLST
HHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHH
RWARERAGALALPLVEVQHHHAHAAACMAEHGLEGPALALCWDGTGLGTDGASWGGELLL
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHH
AEAARFDRLATFRPVPLAGGDRAIRDPWRIALAALLDAFGEDAPLDRLPLFAGVPARELE
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHH
VVRRMLATGLNAPPAHGAGRAFDAAGALALCRPSARFEGQVALALDAAAHGHEAAPYPFD
HHHHHHHCCCCCCCCCCCCCCHHHCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
LDASGPVEALDLRPLWRALAADVLAARPAGEMSARFHAALAAAGAEQVRRAARRTGKLPV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCE
VLTGGCFQNARLAEAIRARLDGEFAVYQHGEVPPGDGGIALGQAVVADAVARA
EEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9799289; 12948488 [H]