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The map label for this gene is rimO [H]

Identifier: 220915232

GI number: 220915232

Start: 123434

End: 124843

Strand: Reverse

Name: rimO [H]

Synonym: A2cp1_0111

Alternate gene names: 220915232

Gene position: 124843-123434 (Counterclockwise)

Preceding gene: 220915233

Following gene: 220915226

Centisome position: 2.48

GC content: 73.19

Gene sequence:

>1410_bases
ATGGCCACCCGCGTCTACATGCACACCCTCGGCTGCCCGAAGAACCGGGTCGACAGCGAGGTGATGCTCGGCACGCTCGC
CGAGGCCGGCTACCGGCTGGTGCAGGACCCGGCGCAGGCCGAGGTCATCGTCGTCAACACCTGCGGCTTCATCGAGAGCG
CCAAGGAGGAGTCGGTCGAGGCCATCGTCGAGCTCGCCGACCAGAAGCGCGAGGGGCGGTGCCGCAAGCTGGTCGTGACC
GGGTGCCTGGTGCAGCGGCACGCCGAGGAGCTCGCCCGCGAGCTGCCCGAGGTCGACCACTTCCTCGGCACCGGCGCCTA
CCAGGACGTGGCCCGGATCGTCTCCGACGCGCAGGCGAAGCGCCTGGTCGTCCCCGACCCCGACTTCGTGCACTCGTCCG
CGACGCCGCGGGTGAACTCGCTGCCGTCGCACACCGCCTACCTCAAGATCGCGGAGGGCTGCGACAACGCCTGCGCGTTC
TGCATCATCCCCAAGCTGCGCGGCGGCCAGCGCTCGCGCCCCATCGACGACCTGGTGGCCGAGGCCGCCGCGCTCGCCGC
GCAGGGGACGGTGGAGCTGTCGCTCGTCGCGCAGGACCTCACCGCGTACGGGCAGGACCTGCCCGGCAAGGTGCGCCTGC
ACCACCTGCTGCCCGAGCTCGCGAAGGTGGACGGCATCCGCTGGATCCGCCTGCACTACGCCTACCCCCGCGACGTGCCC
GACGCGCTGGTGGCCGCGATCGCCGACGAGCCCAAGATCGTGAAGTACCTGGACATGCCGCTCCAGCACAGCTCGGACCG
GCTGCTCCGGGCCATGAAGCGCGGCCGCGACTCGGTGTTCCTGCGCGACCTGCTCGCGCGGCTCCGCTCCCGGATCCCCG
GCCTCGCGCTGCGCACCGCGCTGATCGTCGGCCTGCCCGGCGAGACCGAGGCCGACTTCGAGGACCTGCTCCGCTTCGTG
GAGGAGCAGCGCTTCGAGCGGCTCGGCGTCTTCGAGTACTCCGCCGAGGAGGGCACCCCCGCCGCCGAGATGGCGGACCA
GGTGCCCGACGCGCTGAAGCGCGAGCGGCGCGACCGGATCATGGCCGTGCAGCAGGCGATCTCGCGCGCCCACCAGCAGG
CCATGATCGGCCGCCGCGTCGAGGTGCTGGTGGAGGGTCGCGCCGAGGAGACCGAGCACCTGCTCGCCGGCCGCCACGCC
CAGCAGGCGCCCGAGATCGACGGGCTCACGTACATCAACGACGGCGTGGCGTACCCCGGCGAGATCGTGACCGTGGAGAT
CACCGACGCCGCCGAGTACGACCTCGTCGGCCGCGTGGTCGCCCGCGACCCGTCGCGCGCCGCCCGCCCGCTGCCCGCCG
CCCCGCGCCCCCCCGCGCCGCGCAAGGGCGGCCTGAACGTGCTCGGCTAG

Upstream 100 bases:

>100_bases
GAACTTCCGGGACTGACCCGGCGCCGCGCCGCCCCTCCCCCGGCCTGGGGAGGGGGCTGGATTGCCCCGGGCCCCCCGGT
CTGTTAGATCGCGCCTCCCC

Downstream 100 bases:

>100_bases
CCGGCGCCCGCGTCAACCCTCCCCCCCGCCCCGCCACCGGTCGTCGAGCAAGTCCTCCGCCCGGACGTGATCGGCGAGCT
GCCGATCCGTGAGCAGCACG

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MATRVYMHTLGCPKNRVDSEVMLGTLAEAGYRLVQDPAQAEVIVVNTCGFIESAKEESVEAIVELADQKREGRCRKLVVT
GCLVQRHAEELARELPEVDHFLGTGAYQDVARIVSDAQAKRLVVPDPDFVHSSATPRVNSLPSHTAYLKIAEGCDNACAF
CIIPKLRGGQRSRPIDDLVAEAAALAAQGTVELSLVAQDLTAYGQDLPGKVRLHHLLPELAKVDGIRWIRLHYAYPRDVP
DALVAAIADEPKIVKYLDMPLQHSSDRLLRAMKRGRDSVFLRDLLARLRSRIPGLALRTALIVGLPGETEADFEDLLRFV
EEQRFERLGVFEYSAEEGTPAAEMADQVPDALKRERRDRIMAVQQAISRAHQQAMIGRRVEVLVEGRAEETEHLLAGRHA
QQAPEIDGLTYINDGVAYPGEIVTVEITDAAEYDLVGRVVARDPSRAARPLPAAPRPPAPRKGGLNVLG

Sequences:

>Translated_469_residues
MATRVYMHTLGCPKNRVDSEVMLGTLAEAGYRLVQDPAQAEVIVVNTCGFIESAKEESVEAIVELADQKREGRCRKLVVT
GCLVQRHAEELARELPEVDHFLGTGAYQDVARIVSDAQAKRLVVPDPDFVHSSATPRVNSLPSHTAYLKIAEGCDNACAF
CIIPKLRGGQRSRPIDDLVAEAAALAAQGTVELSLVAQDLTAYGQDLPGKVRLHHLLPELAKVDGIRWIRLHYAYPRDVP
DALVAAIADEPKIVKYLDMPLQHSSDRLLRAMKRGRDSVFLRDLLARLRSRIPGLALRTALIVGLPGETEADFEDLLRFV
EEQRFERLGVFEYSAEEGTPAAEMADQVPDALKRERRDRIMAVQQAISRAHQQAMIGRRVEVLVEGRAEETEHLLAGRHA
QQAPEIDGLTYINDGVAYPGEIVTVEITDAAEYDLVGRVVARDPSRAARPLPAAPRPPAPRKGGLNVLG
>Mature_468_residues
ATRVYMHTLGCPKNRVDSEVMLGTLAEAGYRLVQDPAQAEVIVVNTCGFIESAKEESVEAIVELADQKREGRCRKLVVTG
CLVQRHAEELARELPEVDHFLGTGAYQDVARIVSDAQAKRLVVPDPDFVHSSATPRVNSLPSHTAYLKIAEGCDNACAFC
IIPKLRGGQRSRPIDDLVAEAAALAAQGTVELSLVAQDLTAYGQDLPGKVRLHHLLPELAKVDGIRWIRLHYAYPRDVPD
ALVAAIADEPKIVKYLDMPLQHSSDRLLRAMKRGRDSVFLRDLLARLRSRIPGLALRTALIVGLPGETEADFEDLLRFVE
EQRFERLGVFEYSAEEGTPAAEMADQVPDALKRERRDRIMAVQQAISRAHQQAMIGRRVEVLVEGRAEETEHLLAGRHAQ
QAPEIDGLTYINDGVAYPGEIVTVEITDAAEYDLVGRVVARDPSRAARPLPAAPRPPAPRKGGLNVLG

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=29.1060291060291, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=29.1060291060291, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI93277076, Length=458, Percent_Identity=27.292576419214, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=41.2556053811659, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=30.5986696230599, Blast_Score=215, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=27.2727272727273, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71996771, Length=317, Percent_Identity=30.5993690851735, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21356207, Length=429, Percent_Identity=29.3706293706294, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI19922432, Length=465, Percent_Identity=27.9569892473118, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51570; Mature: 51438

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRVYMHTLGCPKNRVDSEVMLGTLAEAGYRLVQDPAQAEVIVVNTCGFIESAKEESVE
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
AIVELADQKREGRCRKLVVTGCLVQRHAEELARELPEVDHFLGTGAYQDVARIVSDAQAK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHC
RLVVPDPDFVHSSATPRVNSLPSHTAYLKIAEGCDNACAFCIIPKLRGGQRSRPIDDLVA
EEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHH
EAAALAAQGTVELSLVAQDLTAYGQDLPGKVRLHHLLPELAKVDGIRWIRLHYAYPRDVP
HHHHHHHCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCH
DALVAAIADEPKIVKYLDMPLQHSSDRLLRAMKRGRDSVFLRDLLARLRSRIPGLALRTA
HHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHH
LIVGLPGETEADFEDLLRFVEEQRFERLGVFEYSAEEGTPAAEMADQVPDALKRERRDRI
HEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
MAVQQAISRAHQQAMIGRRVEVLVEGRAEETEHLLAGRHAQQAPEIDGLTYINDGVAYPG
HHHHHHHHHHHHHHHHHCHHHEEECCCCHHHHHHHHCCHHHCCCCCCCCEEECCCCCCCC
EIVTVEITDAAEYDLVGRVVARDPSRAARPLPAAPRPPAPRKGGLNVLG
CEEEEEECCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATRVYMHTLGCPKNRVDSEVMLGTLAEAGYRLVQDPAQAEVIVVNTCGFIESAKEESVE
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
AIVELADQKREGRCRKLVVTGCLVQRHAEELARELPEVDHFLGTGAYQDVARIVSDAQAK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHC
RLVVPDPDFVHSSATPRVNSLPSHTAYLKIAEGCDNACAFCIIPKLRGGQRSRPIDDLVA
EEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHH
EAAALAAQGTVELSLVAQDLTAYGQDLPGKVRLHHLLPELAKVDGIRWIRLHYAYPRDVP
HHHHHHHCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCH
DALVAAIADEPKIVKYLDMPLQHSSDRLLRAMKRGRDSVFLRDLLARLRSRIPGLALRTA
HHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHH
LIVGLPGETEADFEDLLRFVEEQRFERLGVFEYSAEEGTPAAEMADQVPDALKRERRDRI
HEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
MAVQQAISRAHQQAMIGRRVEVLVEGRAEETEHLLAGRHAQQAPEIDGLTYINDGVAYPG
HHHHHHHHHHHHHHHHHCHHHEEECCCCHHHHHHHHCCHHHCCCCCCCCEEECCCCCCCC
EIVTVEITDAAEYDLVGRVVARDPSRAARPLPAAPRPPAPRKGGLNVLG
CEEEEEECCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA