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The map label for this gene is bamHIM [H]

Identifier: 220905504

GI number: 220905504

Start: 2702638

End: 2703495

Strand: Reverse

Name: bamHIM [H]

Synonym: Ddes_2243

Alternate gene names: 220905504

Gene position: 2703495-2702638 (Counterclockwise)

Preceding gene: 220905506

Following gene: 220905502

Centisome position: 94.09

GC content: 35.43

Gene sequence:

>858_bases
ATGAACAATAATGACTTAACTGATGACTTATTCTTGACTTCACCCACAATAACACCTCATTTTCAAACCAAATATGGAAT
CATGCTAGAAGGTGACTGCATAAATATTTTAAAGAATATTAAATCAAATAGTGTGGATACATTTTTTGCAGATCCACCAT
TTAATCTAAATAAAAAATATGGTGTAAAGTCAAACGATAACAAGTATGATAAAGAATATATAGAATGGTGTTATAGTTGG
ATTTCTGAAGGAATTCGCATTTTAAAGCCAGGTGGTGCCTTCTTTATATATAACTTGCCAAAATGGAACATGATCCTAGG
GAGTTATTTGACTTCAGCAGGACTGCTGTTTCGGCATTGGATTGCTGTAAGCATGAAGCTTTCCCTTCCAATTCCCAAGC
GGTTATATCCCGCACATTATTCACTGCTTTACTTTACAAAAGGTGCCCCAAAAACATTTAACAAAATTAGAACGCCAATA
GAACTATGTAGACATTGTGGAAAGGAAATCAAGGATTATGGCGGACACCGCAAATCAATGAACCCCAATGGAGTAAATTT
ATCTGATGTTTGGAATGACATTGCACCTGTTCGCCACCACAAATTCAAACACAACAAAAGGACTGAAAATCAACTTTCCA
CAAAATTACTTGAACGAATAATACTAATGACAACAAATGAAGGTGATATTGTTGTTGATCCATTTGGTGGCGGTGGCACC
ACTTTTGCTGTGTGTGAAGCCAAGCTCCGCAGATGGATAGGTATAGAAATAGAAGACATCACCCCTATCAAAGAAAGAAT
CGAATTAAAATATGATTTGCATTACAATAGTGATTATGTTGAAGAGTCTTCAAAATAG

Upstream 100 bases:

>100_bases
CAAGCATCACACTCAATTATTAGGTAATTTTATGAATATTGCTCGCAATGTGTACTTTTTTAGTGTGTTTATTTAATTTA
GAGATATTAATGGAGGACAT

Downstream 100 bases:

>100_bases
CGCTCTGCCATCGTTCCCTTTCTGAAACCGAGGGGCTGATGTGGACGTTGTGTCATGTTCAATTCCTAATATATATAGTG
CTCCTTCATGACAATTGTAT

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.BamHI; N(4)- cytosine-specific methyltransferase BamHI [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNNNDLTDDLFLTSPTITPHFQTKYGIMLEGDCINILKNIKSNSVDTFFADPPFNLNKKYGVKSNDNKYDKEYIEWCYSW
ISEGIRILKPGGAFFIYNLPKWNMILGSYLTSAGLLFRHWIAVSMKLSLPIPKRLYPAHYSLLYFTKGAPKTFNKIRTPI
ELCRHCGKEIKDYGGHRKSMNPNGVNLSDVWNDIAPVRHHKFKHNKRTENQLSTKLLERIILMTTNEGDIVVDPFGGGGT
TFAVCEAKLRRWIGIEIEDITPIKERIELKYDLHYNSDYVEESSK

Sequences:

>Translated_285_residues
MNNNDLTDDLFLTSPTITPHFQTKYGIMLEGDCINILKNIKSNSVDTFFADPPFNLNKKYGVKSNDNKYDKEYIEWCYSW
ISEGIRILKPGGAFFIYNLPKWNMILGSYLTSAGLLFRHWIAVSMKLSLPIPKRLYPAHYSLLYFTKGAPKTFNKIRTPI
ELCRHCGKEIKDYGGHRKSMNPNGVNLSDVWNDIAPVRHHKFKHNKRTENQLSTKLLERIILMTTNEGDIVVDPFGGGGT
TFAVCEAKLRRWIGIEIEDITPIKERIELKYDLHYNSDYVEESSK
>Mature_285_residues
MNNNDLTDDLFLTSPTITPHFQTKYGIMLEGDCINILKNIKSNSVDTFFADPPFNLNKKYGVKSNDNKYDKEYIEWCYSW
ISEGIRILKPGGAFFIYNLPKWNMILGSYLTSAGLLFRHWIAVSMKLSLPIPKRLYPAHYSLLYFTKGAPKTFNKIRTPI
ELCRHCGKEIKDYGGHRKSMNPNGVNLSDVWNDIAPVRHHKFKHNKRTENQLSTKLLERIILMTTNEGDIVVDPFGGGGT
TFAVCEAKLRRWIGIEIEDITPIKERIELKYDLHYNSDYVEESSK

Specific function: This methylase recognizes the double-stranded sequence GGATCC, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BamHI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=240, Percent_Identity=32.0833333333333, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 32892; Mature: 32892

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNDLTDDLFLTSPTITPHFQTKYGIMLEGDCINILKNIKSNSVDTFFADPPFNLNKKY
CCCCCCCCCEEEECCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCCC
GVKSNDNKYDKEYIEWCYSWISEGIRILKPGGAFFIYNLPKWNMILGSYLTSAGLLFRHW
CCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
IAVSMKLSLPIPKRLYPAHYSLLYFTKGAPKTFNKIRTPIELCRHCGKEIKDYGGHRKSM
HHHEEEECCCCCHHHCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHCHHHHHCCCCCCCC
NPNGVNLSDVWNDIAPVRHHKFKHNKRTENQLSTKLLERIILMTTNEGDIVVDPFGGGGT
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCC
TFAVCEAKLRRWIGIEIEDITPIKERIELKYDLHYNSDYVEESSK
CHHHHHHHHHHHHCCEEECCCCHHHHEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNNNDLTDDLFLTSPTITPHFQTKYGIMLEGDCINILKNIKSNSVDTFFADPPFNLNKKY
CCCCCCCCCEEEECCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCCC
GVKSNDNKYDKEYIEWCYSWISEGIRILKPGGAFFIYNLPKWNMILGSYLTSAGLLFRHW
CCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
IAVSMKLSLPIPKRLYPAHYSLLYFTKGAPKTFNKIRTPIELCRHCGKEIKDYGGHRKSM
HHHEEEECCCCCHHHCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHCHHHHHCCCCCCCC
NPNGVNLSDVWNDIAPVRHHKFKHNKRTENQLSTKLLERIILMTTNEGDIVVDPFGGGGT
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCC
TFAVCEAKLRRWIGIEIEDITPIKERIELKYDLHYNSDYVEESSK
CHHHHHHHHHHHHCCEEECCCCHHHHEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1901989; 2235511 [H]