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The map label for this gene is cbiH [H]

Identifier: 220905276

GI number: 220905276

Start: 2427232

End: 2427993

Strand: Reverse

Name: cbiH [H]

Synonym: Ddes_2014

Alternate gene names: 220905276

Gene position: 2427993-2427232 (Counterclockwise)

Preceding gene: 220905277

Following gene: 220905275

Centisome position: 84.5

GC content: 61.68

Gene sequence:

>762_bases
ATGACGCAAGCCGCCAGTCTTCATGTTGTCGGCCTTGGCCCCGGCGACGCTCAATGCCTCACGCCGCAGGCCCGCGCAGT
GCTGGCCCAGGCCAGTTGTGTGGTGGGCTATCACCTGTACATGGATATGGTTCCCCCCGAATTGCTGGAGGGCAAAGAGC
TGATAAGCACCGGCATGCGCCACGAAACAGAGCGCTGCGCGGCCGCTGTGGATGCGGCCCTGGCCGGTCGGCCCACGGCC
CTTGTCTGCTCGGGCGACCCCGGTATCTACGCCATGGCGGGCCTTGCCCTGGAAATACTTGAACTGCGAGGCCTGGCCGA
TACGGTCCCCTTTGGTGTGGTTCCGGGCGTTCCCGCGGTATGCGCGGCTGCGGCTCTGCTGGGCGCACCCCTGACGCATG
ATTTTGCCTGCGTGAGCCTGAGCGATCTGCTGACGCCGTGGGAAACCATTGTTAAAAGGCTTACCGCGGCTCTGGAGGCG
GATTTTGTGTGCGTTATCTACAATCCGCGCTCCAGGGGGCGCCCCGGCCATCTGGACCATGCGCTGGAACTGGCCCGCCG
TTTTCGCGCGGGGGAATGCCCTGTGGGGCTGGTGCGGCAGGCATACAGGCCTGGACAGAGTGTGAGCGTGCACAGACTGG
ATGCCTTTGATGCCGCACAGGTGGATATGCTTTCTTTGCTGATTATCGGCAACAATGAAAGCAGATCTGTTGGGTCGTAT
ATGCTTACACCCAGGGGATATGCACGTAAAAGACGTTTATGA

Upstream 100 bases:

>100_bases
CCATTTTTCAGTCGCAGTTGACCGTAGCTGTGGCCCTGGCCCTCGAGCAGGAGCCGGACAAGGCCGCAAACGGTGAAAAC
AACAAAAAAGAAGAACAGAT

Downstream 100 bases:

>100_bases
CAGTGTAAATGACTTGAAAATGTGTTTCGGGCTTCCCATTGACACGTTGCCATGCTGGCCGTAAGCTTGCAAAAATTCTC
ATGTGTTGTTTTTTTACCTT

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTQAASLHVVGLGPGDAQCLTPQARAVLAQASCVVGYHLYMDMVPPELLEGKELISTGMRHETERCAAAVDAALAGRPTA
LVCSGDPGIYAMAGLALEILELRGLADTVPFGVVPGVPAVCAAAALLGAPLTHDFACVSLSDLLTPWETIVKRLTAALEA
DFVCVIYNPRSRGRPGHLDHALELARRFRAGECPVGLVRQAYRPGQSVSVHRLDAFDAAQVDMLSLLIIGNNESRSVGSY
MLTPRGYARKRRL

Sequences:

>Translated_253_residues
MTQAASLHVVGLGPGDAQCLTPQARAVLAQASCVVGYHLYMDMVPPELLEGKELISTGMRHETERCAAAVDAALAGRPTA
LVCSGDPGIYAMAGLALEILELRGLADTVPFGVVPGVPAVCAAAALLGAPLTHDFACVSLSDLLTPWETIVKRLTAALEA
DFVCVIYNPRSRGRPGHLDHALELARRFRAGECPVGLVRQAYRPGQSVSVHRLDAFDAAQVDMLSLLIIGNNESRSVGSY
MLTPRGYARKRRL
>Mature_252_residues
TQAASLHVVGLGPGDAQCLTPQARAVLAQASCVVGYHLYMDMVPPELLEGKELISTGMRHETERCAAAVDAALAGRPTAL
VCSGDPGIYAMAGLALEILELRGLADTVPFGVVPGVPAVCAAAALLGAPLTHDFACVSLSDLLTPWETIVKRLTAALEAD
FVCVIYNPRSRGRPGHLDHALELARRFRAGECPVGLVRQAYRPGQSVSVHRLDAFDAAQVDMLSLLIIGNNESRSVGSYM
LTPRGYARKRRL

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 26883; Mature: 26751

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAASLHVVGLGPGDAQCLTPQARAVLAQASCVVGYHLYMDMVPPELLEGKELISTGMR
CCCCCEEEEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHH
HETERCAAAVDAALAGRPTALVCSGDPGIYAMAGLALEILELRGLADTVPFGVVPGVPAV
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHH
CAAAALLGAPLTHDFACVSLSDLLTPWETIVKRLTAALEADFVCVIYNPRSRGRPGHLDH
HHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHH
ALELARRFRAGECPVGLVRQAYRPGQSVSVHRLDAFDAAQVDMLSLLIIGNNESRSVGSY
HHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHEEEEEEEECCCCCCCCCCE
MLTPRGYARKRRL
EECCCCHHHHCCC
>Mature Secondary Structure 
TQAASLHVVGLGPGDAQCLTPQARAVLAQASCVVGYHLYMDMVPPELLEGKELISTGMR
CCCCEEEEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHH
HETERCAAAVDAALAGRPTALVCSGDPGIYAMAGLALEILELRGLADTVPFGVVPGVPAV
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHH
CAAAALLGAPLTHDFACVSLSDLLTPWETIVKRLTAALEADFVCVIYNPRSRGRPGHLDH
HHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHH
ALELARRFRAGECPVGLVRQAYRPGQSVSVHRLDAFDAAQVDMLSLLIIGNNESRSVGSY
HHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHEEEEEEEECCCCCCCCCCE
MLTPRGYARKRRL
EECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]