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The map label for this gene is cysS

Identifier: 220904715

GI number: 220904715

Start: 1734350

End: 1735801

Strand: Direct

Name: cysS

Synonym: Ddes_1447

Alternate gene names: 220904715

Gene position: 1734350-1735801 (Clockwise)

Preceding gene: 220904714

Following gene: 220904716

Centisome position: 60.36

GC content: 58.95

Gene sequence:

>1452_bases
ATGCTGCTCTACAATACCCTGGGCCGCCAGAAAGAAGAATTCACTCCCCTCCGCCCCGGCAAGGTACACATGTACGTCTG
CGGCATCACCGCTTACGACTATTGCCACATCGGCCATGCCCGGTCGGCCCTGGTTTTTGACGTACTTGTGCGCCAGTTGC
GCCATATGGGCCTGGATGTGACCTTTGTACGCAATTTTACGGACGTGGACGACAAGATCATCAACCGGGCCAACAAAGAA
GGGCTTGACTGGCGCGAAGTGGCCCAGACCTATATCAACGCCTTTTATGAAGATATGGACAGGCTCGGCGTGCAGCGCGC
GGACGTGGAACCCCGGGCCACGGACCATATACAGGAAATTCAAGACCTCTGCGCCAAGCTGGTAGCTGAAGGCAAGGCCT
ATGCCACGACATCGGGCGACGTGTATTTCAGGGTGCGCTCCTACCCGCCCTACGGCAAGCTCTCGGGCCGCAGCCTGGAC
GAGCTGCTTTCCGGCGCGCGCGTGGCCCCCGGCGAAGAAAAGGAAGATCCGCTGGACTTCGCCCTGTGGAAGGCCGCCAA
GCCCGGCGAACCCTCCTGGGAAAGCCCCTGGGGTCCGGGCAGGCCCGGCTGGCACATTGAATGCTCGGCCATGAGCGAAT
CTTACCTGCCGCTGGATATCCACGGCGGCGGGCAGGACCTGGTGTTTCCCCACCACGAAAACGAAATCGCCCAGACAGAG
GCCGTGTGCCATTGCCATCTGGCCCGGTATTGGGTGCACAACGGCTTTGTGCAGGTCAATGCCGAAAAAATGTCAAAATC
ACTTGGCAACTTCAAGACCATCCGCGATATTCTTGAAAGCTACCTGCCAGAAACATTGCGCTTTTTTCTTCTCGGCAAGC
ATTATCGCAGCCCCATTGACTTCACTGCGGAGGGTATGGACGAGGCGGAAAAAGCCTTGCACAGAGTGTATACCGCCCTG
CTGGAAACGCAAAAAGCCCTCACGCGCGAAAAATGGAAAAAATCTCCCCTGCCCGCGCAACTGACAGAAGATTGGGCCGC
ACAGGCCGAAGCTCTGGACGAGGCAATGAATGACGACCTCAACACCGCGCAGGCTCTGGGCCATATCTTCACACAGGTAC
GCCTTGTAAACCGGTTGCTGGAAGACAAAACACTGCGTGCCGCCGAGGCAGGACGGGATCTTTTGCAGGACTTTCTGGCC
CGGGCAGAACAGTGGAATACGCGACTGGGGCTTTTCGGCCAGCAGCCTGAAGCGTTTCTGGCCGACCTGCGCCGCATTCG
CGCCGCCCGCAGAAACATTGACATCCCCCGCGTGGAGGCTCTGCTGCAAGAACGGCAGGAAGCCCGCGCAAACAAGGATT
TCGCCCGTTCCGACGCTCTGCGTCAGGCGCTGCTTGATCTCGGGGTTAGCGTACAAGACACCCCCGAAGGGCAGAACTGG
GACCTGGAGTAG

Upstream 100 bases:

>100_bases
GGCGCAGGCCATAGTGGATGCTTTTCTGGAAGCCCCTTTTGAAGGCGGCCGCCACCAGCGTCGCCTGGATATGCTTAACC
TCCCCGCATAAAGGAAGATC

Downstream 100 bases:

>100_bases
GCTCGGCCCGGGTACCGACGGGAGAACCTATGCTGCATTATTGCGCCCCGCGCCGCTGGACGGCCCTGCTTGTGTTGCTG
GCTTTTCTGCTGCCGCAGAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MLLYNTLGRQKEEFTPLRPGKVHMYVCGITAYDYCHIGHARSALVFDVLVRQLRHMGLDVTFVRNFTDVDDKIINRANKE
GLDWREVAQTYINAFYEDMDRLGVQRADVEPRATDHIQEIQDLCAKLVAEGKAYATTSGDVYFRVRSYPPYGKLSGRSLD
ELLSGARVAPGEEKEDPLDFALWKAAKPGEPSWESPWGPGRPGWHIECSAMSESYLPLDIHGGGQDLVFPHHENEIAQTE
AVCHCHLARYWVHNGFVQVNAEKMSKSLGNFKTIRDILESYLPETLRFFLLGKHYRSPIDFTAEGMDEAEKALHRVYTAL
LETQKALTREKWKKSPLPAQLTEDWAAQAEALDEAMNDDLNTAQALGHIFTQVRLVNRLLEDKTLRAAEAGRDLLQDFLA
RAEQWNTRLGLFGQQPEAFLADLRRIRAARRNIDIPRVEALLQERQEARANKDFARSDALRQALLDLGVSVQDTPEGQNW
DLE

Sequences:

>Translated_483_residues
MLLYNTLGRQKEEFTPLRPGKVHMYVCGITAYDYCHIGHARSALVFDVLVRQLRHMGLDVTFVRNFTDVDDKIINRANKE
GLDWREVAQTYINAFYEDMDRLGVQRADVEPRATDHIQEIQDLCAKLVAEGKAYATTSGDVYFRVRSYPPYGKLSGRSLD
ELLSGARVAPGEEKEDPLDFALWKAAKPGEPSWESPWGPGRPGWHIECSAMSESYLPLDIHGGGQDLVFPHHENEIAQTE
AVCHCHLARYWVHNGFVQVNAEKMSKSLGNFKTIRDILESYLPETLRFFLLGKHYRSPIDFTAEGMDEAEKALHRVYTAL
LETQKALTREKWKKSPLPAQLTEDWAAQAEALDEAMNDDLNTAQALGHIFTQVRLVNRLLEDKTLRAAEAGRDLLQDFLA
RAEQWNTRLGLFGQQPEAFLADLRRIRAARRNIDIPRVEALLQERQEARANKDFARSDALRQALLDLGVSVQDTPEGQNW
DLE
>Mature_483_residues
MLLYNTLGRQKEEFTPLRPGKVHMYVCGITAYDYCHIGHARSALVFDVLVRQLRHMGLDVTFVRNFTDVDDKIINRANKE
GLDWREVAQTYINAFYEDMDRLGVQRADVEPRATDHIQEIQDLCAKLVAEGKAYATTSGDVYFRVRSYPPYGKLSGRSLD
ELLSGARVAPGEEKEDPLDFALWKAAKPGEPSWESPWGPGRPGWHIECSAMSESYLPLDIHGGGQDLVFPHHENEIAQTE
AVCHCHLARYWVHNGFVQVNAEKMSKSLGNFKTIRDILESYLPETLRFFLLGKHYRSPIDFTAEGMDEAEKALHRVYTAL
LETQKALTREKWKKSPLPAQLTEDWAAQAEALDEAMNDDLNTAQALGHIFTQVRLVNRLLEDKTLRAAEAGRDLLQDFLA
RAEQWNTRLGLFGQQPEAFLADLRRIRAARRNIDIPRVEALLQERQEARANKDFARSDALRQALLDLGVSVQDTPEGQNW
DLE

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=505, Percent_Identity=35.049504950495, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI10835051, Length=418, Percent_Identity=32.2966507177034, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI21269877, Length=418, Percent_Identity=32.2966507177034, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI62240992, Length=418, Percent_Identity=32.2966507177034, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI303304987, Length=418, Percent_Identity=32.2966507177034, Blast_Score=185, Evalue=1e-46,
Organism=Escherichia coli, GI1786737, Length=484, Percent_Identity=46.6942148760331, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17509795, Length=439, Percent_Identity=31.4350797266515, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17509797, Length=439, Percent_Identity=31.4350797266515, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6324082, Length=305, Percent_Identity=36.0655737704918, Blast_Score=179, Evalue=8e-46,
Organism=Drosophila melanogaster, GI19922218, Length=517, Percent_Identity=34.0425531914894, Blast_Score=250, Evalue=2e-66,
Organism=Drosophila melanogaster, GI19922364, Length=320, Percent_Identity=35, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_DESDA (B8J0S2)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480027.1
- GeneID:   7285143
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1447
- HOGENOM:   HBG327651
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54988; Mature: 54988

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLYNTLGRQKEEFTPLRPGKVHMYVCGITAYDYCHIGHARSALVFDVLVRQLRHMGLDV
CCCCCCCCCCHHHCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCE
TFVRNFTDVDDKIINRANKEGLDWREVAQTYINAFYEDMDRLGVQRADVEPRATDHIQEI
EEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
QDLCAKLVAEGKAYATTSGDVYFRVRSYPPYGKLSGRSLDELLSGARVAPGEEKEDPLDF
HHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHH
ALWKAAKPGEPSWESPWGPGRPGWHIECSAMSESYLPLDIHGGGQDLVFPHHENEIAQTE
HHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHH
AVCHCHLARYWVHNGFVQVNAEKMSKSLGNFKTIRDILESYLPETLRFFLLGKHYRSPID
HHHHHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FTAEGMDEAEKALHRVYTALLETQKALTREKWKKSPLPAQLTEDWAAQAEALDEAMNDDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCH
NTAQALGHIFTQVRLVNRLLEDKTLRAAEAGRDLLQDFLARAEQWNTRLGLFGQQPEAFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ADLRRIRAARRNIDIPRVEALLQERQEARANKDFARSDALRQALLDLGVSVQDTPEGQNW
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DLE
CCC
>Mature Secondary Structure
MLLYNTLGRQKEEFTPLRPGKVHMYVCGITAYDYCHIGHARSALVFDVLVRQLRHMGLDV
CCCCCCCCCCHHHCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCE
TFVRNFTDVDDKIINRANKEGLDWREVAQTYINAFYEDMDRLGVQRADVEPRATDHIQEI
EEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
QDLCAKLVAEGKAYATTSGDVYFRVRSYPPYGKLSGRSLDELLSGARVAPGEEKEDPLDF
HHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHH
ALWKAAKPGEPSWESPWGPGRPGWHIECSAMSESYLPLDIHGGGQDLVFPHHENEIAQTE
HHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHH
AVCHCHLARYWVHNGFVQVNAEKMSKSLGNFKTIRDILESYLPETLRFFLLGKHYRSPID
HHHHHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FTAEGMDEAEKALHRVYTALLETQKALTREKWKKSPLPAQLTEDWAAQAEALDEAMNDDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCH
NTAQALGHIFTQVRLVNRLLEDKTLRAAEAGRDLLQDFLARAEQWNTRLGLFGQQPEAFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ADLRRIRAARRNIDIPRVEALLQERQEARANKDFARSDALRQALLDLGVSVQDTPEGQNW
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DLE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA