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The map label for this gene is sigB [H]

Identifier: 220904711

GI number: 220904711

Start: 1729654

End: 1730955

Strand: Direct

Name: sigB [H]

Synonym: Ddes_1443

Alternate gene names: 220904711

Gene position: 1729654-1730955 (Clockwise)

Preceding gene: 220904706

Following gene: 220904712

Centisome position: 60.19

GC content: 55.76

Gene sequence:

>1302_bases
ATGAAAAAAAATAGCAAGATAGCCCCGAGCAAAACCAAGGGCGCCGCAGCAGCGGAAAAAAGCAGAAAAACCACGCTTCC
GGTGGAGGTTGTCGGGCCTGAAGATGCCCCCAGGCAGCCCTCTGCGGCTGCCGGAAAAACACGAACCCGCGTTGCCGCAG
ACACTGCCGCCAAAACATCCGCCGGGAACGCCGGCAAAAGCCGAAAAAAAGAAACGTCTTCAGCTCTTGAGCCCGACGAT
GGCAGCCGCAGCTCCGGCACGGTGCTGGATGTGGACGAACTTGAACATGAGCGCGATACGGACGAGCTTGATATCGAATC
GCCCGACAGCGAAGAAGAACTGCTCGACTCCGACATGGACGAAGACGATGAGCCGCGCCTGACAGATGAAGATGAACAGG
ACCGCAGCGTTCCCGCGCCCTCTTCTTCACGGGTGCCTGCCGTGGGTACTCGCGACAGCCTGCACCTGTATCTTCGCGAA
GTAAGCCGCTTCCCCATGCTTAAACCCGAAGAGGAGCATGAACTGGCTGTGCGCGTGCGCGATCATAATGATGCGGACGC
CGCCTTTCGCCTGGTTTCATCCCACCTGCGGCTGGTCGTGCGCATCGCCATGGATTTTCAGCGCCGCTGGATGCAGAATG
TGCTTGACCTTGTGCAAGAAGGAAATGTGGGCCTCATGCGCGCGGTCAACAAGTTTGACCCCGACAAGGGCATCAAGTTT
TCCTATTATGCCTCGTTCTGGATCAAAGCCTATATTCTCAAGTTCATCATGGACAACTGGCGCATGGTCAAGATCGGCAC
GACCCAGGTGCAGCGCAAGCTGTTCTACAACCTGAACAGAGAACGCCAGAAGCTCATCATGCAGGGCTACGACCCGGATG
CCGCCATGCTTTCGGAACGCCTGGGCGTAACCGAAGAGCAGATAAATGAAATGGACCAGCGCCTTGCCTCTACAGACATG
TCGCTTAACGCCCAGGTGGGCGAAGATTCTGGCGGCGCCACCCGCATGGACTTTCTGCCAGCCCTGGGACCAGGGATCGA
AGACAGTCTGGCATCTACGGAAATAGCGGGAATTGTGCGCTCAAAGCTGAAAACCATCATGCCGAAGCTCAACGAGAAAG
AACAGTATATCCTGCACAACAGGCTGCTTTCTGACGAGCCGATCACCTTGCGTGAGATAGGCGAACGGTATAACATCACA
AGAGAAAGAGTGCGCCAGCTTGAGGCGCGCCTGGTGGAAAAAATTGGCCAGCACATGAAAAATGACATCGAAGACTTCTC
CGATCACTGGATTCAATCCTAG

Upstream 100 bases:

>100_bases
TCTTTGCCCCCGATGAATAATATTGAAAAGAACAAACCAAAACTATAGCATACAGACATTGCAGCGCTTTTGGAAGCGCT
AATGCCTGAGTATGGATAAA

Downstream 100 bases:

>100_bases
AGCATTTCACGTATGAAACGCTCTGCAAGGGTTTGCCTTCAAATCCTTGCCGCCAAATGATTGCAGGACGACTTTGCCGG
CCGGTAAGCACTCATTTGAG

Product: putative RNA polymerase, sigma 70 family subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKKNSKIAPSKTKGAAAAEKSRKTTLPVEVVGPEDAPRQPSAAAGKTRTRVAADTAAKTSAGNAGKSRKKETSSALEPDD
GSRSSGTVLDVDELEHERDTDELDIESPDSEEELLDSDMDEDDEPRLTDEDEQDRSVPAPSSSRVPAVGTRDSLHLYLRE
VSRFPMLKPEEEHELAVRVRDHNDADAAFRLVSSHLRLVVRIAMDFQRRWMQNVLDLVQEGNVGLMRAVNKFDPDKGIKF
SYYASFWIKAYILKFIMDNWRMVKIGTTQVQRKLFYNLNRERQKLIMQGYDPDAAMLSERLGVTEEQINEMDQRLASTDM
SLNAQVGEDSGGATRMDFLPALGPGIEDSLASTEIAGIVRSKLKTIMPKLNEKEQYILHNRLLSDEPITLREIGERYNIT
RERVRQLEARLVEKIGQHMKNDIEDFSDHWIQS

Sequences:

>Translated_433_residues
MKKNSKIAPSKTKGAAAAEKSRKTTLPVEVVGPEDAPRQPSAAAGKTRTRVAADTAAKTSAGNAGKSRKKETSSALEPDD
GSRSSGTVLDVDELEHERDTDELDIESPDSEEELLDSDMDEDDEPRLTDEDEQDRSVPAPSSSRVPAVGTRDSLHLYLRE
VSRFPMLKPEEEHELAVRVRDHNDADAAFRLVSSHLRLVVRIAMDFQRRWMQNVLDLVQEGNVGLMRAVNKFDPDKGIKF
SYYASFWIKAYILKFIMDNWRMVKIGTTQVQRKLFYNLNRERQKLIMQGYDPDAAMLSERLGVTEEQINEMDQRLASTDM
SLNAQVGEDSGGATRMDFLPALGPGIEDSLASTEIAGIVRSKLKTIMPKLNEKEQYILHNRLLSDEPITLREIGERYNIT
RERVRQLEARLVEKIGQHMKNDIEDFSDHWIQS
>Mature_433_residues
MKKNSKIAPSKTKGAAAAEKSRKTTLPVEVVGPEDAPRQPSAAAGKTRTRVAADTAAKTSAGNAGKSRKKETSSALEPDD
GSRSSGTVLDVDELEHERDTDELDIESPDSEEELLDSDMDEDDEPRLTDEDEQDRSVPAPSSSRVPAVGTRDSLHLYLRE
VSRFPMLKPEEEHELAVRVRDHNDADAAFRLVSSHLRLVVRIAMDFQRRWMQNVLDLVQEGNVGLMRAVNKFDPDKGIKF
SYYASFWIKAYILKFIMDNWRMVKIGTTQVQRKLFYNLNRERQKLIMQGYDPDAAMLSERLGVTEEQINEMDQRLASTDM
SLNAQVGEDSGGATRMDFLPALGPGIEDSLASTEIAGIVRSKLKTIMPKLNEKEQYILHNRLLSDEPITLREIGERYNIT
RERVRQLEARLVEKIGQHMKNDIEDFSDHWIQS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=275, Percent_Identity=37.8181818181818, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789098, Length=263, Percent_Identity=32.319391634981, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1789448, Length=235, Percent_Identity=34.0425531914894, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 48915; Mature: 48915

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNSKIAPSKTKGAAAAEKSRKTTLPVEVVGPEDAPRQPSAAAGKTRTRVAADTAAKTS
CCCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCC
AGNAGKSRKKETSSALEPDDGSRSSGTVLDVDELEHERDTDELDIESPDSEEELLDSDMD
CCCCCCHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCHHHHHHCCCC
EDDEPRLTDEDEQDRSVPAPSSSRVPAVGTRDSLHLYLREVSRFPMLKPEEEHELAVRVR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
DHNDADAAFRLVSSHLRLVVRIAMDFQRRWMQNVLDLVQEGNVGLMRAVNKFDPDKGIKF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEE
SYYASFWIKAYILKFIMDNWRMVKIGTTQVQRKLFYNLNRERQKLIMQGYDPDAAMLSER
HHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHH
LGVTEEQINEMDQRLASTDMSLNAQVGEDSGGATRMDFLPALGPGIEDSLASTEIAGIVR
HCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHH
SKLKTIMPKLNEKEQYILHNRLLSDEPITLREIGERYNITRERVRQLEARLVEKIGQHMK
HHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
NDIEDFSDHWIQS
HHHHHHHHHHCCC
>Mature Secondary Structure
MKKNSKIAPSKTKGAAAAEKSRKTTLPVEVVGPEDAPRQPSAAAGKTRTRVAADTAAKTS
CCCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCC
AGNAGKSRKKETSSALEPDDGSRSSGTVLDVDELEHERDTDELDIESPDSEEELLDSDMD
CCCCCCHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCHHHHHHCCCC
EDDEPRLTDEDEQDRSVPAPSSSRVPAVGTRDSLHLYLREVSRFPMLKPEEEHELAVRVR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
DHNDADAAFRLVSSHLRLVVRIAMDFQRRWMQNVLDLVQEGNVGLMRAVNKFDPDKGIKF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEE
SYYASFWIKAYILKFIMDNWRMVKIGTTQVQRKLFYNLNRERQKLIMQGYDPDAAMLSER
HHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHH
LGVTEEQINEMDQRLASTDMSLNAQVGEDSGGATRMDFLPALGPGIEDSLASTEIAGIVR
HCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHH
SKLKTIMPKLNEKEQYILHNRLLSDEPITLREIGERYNITRERVRQLEARLVEKIGQHMK
HHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
NDIEDFSDHWIQS
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1475207 [H]