Click here to switch to the map view.

The map label for this gene is ybiW [H]

Identifier: 220904704

GI number: 220904704

Start: 1718817

End: 1721312

Strand: Reverse

Name: ybiW [H]

Synonym: Ddes_1436

Alternate gene names: 220904704

Gene position: 1721312-1718817 (Counterclockwise)

Preceding gene: 220904707

Following gene: 220904703

Centisome position: 59.9

GC content: 56.61

Gene sequence:

>2496_bases
ATGAGCATGACCACTTGCGAATGCCGGTCCCCCCAGGAGCAGCGCCTGTACGACAAAATTGAAGGTCGGGAAGACAGGTT
CCGTAAGACCCATCCCCGGGTTTTCAGGCTGCTGGAGCGGTTTGAAGGGCAGAAGCCGCGCATTGATATCGAGCGCGCGC
TGTACTTTACCCAGTCCATGCAGGAAACCGAAGGGCAGCCGCTGGTACTGCGCTGGGCCAAGGCCCTCATGCACATTGCC
CGCAACATGACCGTCTATGTGCAGGAAGATCAGTTGCTGCTTGGCCGCGCCGGTTGCGACGGGCGCTACGGCATCCTTTA
TCCGGAGCTGGACGGTGATTTTCTTGATATCGCCGTGCGCGATCTGCCCACCCGCAAAACTTCACCGGCTACCATCACGC
CTGAAGACGCCAGGCGCGTCGTGGAAGAGATCGCCCCGTACTGGAAGGGCAAGACCTACCATGAAGCGCTGAATGCGGCC
CTGCCCGCCGAAGTGCACAAGCTTACCTATGATGACCCCGAAGGCCTGATTTCGCGTTTTATCGTTAATGAAACCTCTTC
GTTCCGCTCTTCCATCCAGTGGGTTCACGACTACGAAAAGATCCTCAAGCGCGGCTTTAACAGCATCAAGAAAGAAGCCC
GGGAAAAACTGGCGGCTCTTGATCCGCTGAGCGCCAAGGATGACCGCGAAAAGCGTCCCTTCCTTGAAGCTGTCATGATA
GTGTGTGACGCCATTGTGCTCTGGGCCAAACGTCACGCAGTGCTTGCCCGCGAAATGGCCGAAAAAGAATCCGATCCCGT
GCGTAAGGCTGAATTGCTGCGCATGGCAGAAAACGCCGAGCACGTTCCCGGTGAGCCTGCCCGCGACTTCTGGGAAGCCT
GCCAGAGCCAGTGGTTTACCCAGATGTTCTCGCGCATCGAGCAGAAGACCGGCACCACCATTTCCAACGGCCGCATGGAC
CAGTACTTTCAGCCCTACTACAAGCAGGACCGTGAGGCCGGGAAGATTACCGAAGCCCAGGCCATGGAGCTGCTGGAATG
CATGTGGGTGGGCATGGCCGAATTTATTGACATGTACATCTCTCCCACCGGCGGCGCCTTTAACGAAGGCTACGCCCACT
GGGAAGCGGTCACCGTTGGCGGGCAGACCCCGGACGGACGCGATGCCAGCAATGACCTTACCTATCTGATTCTCAAGTCC
AAGCGGGAATTTCCGTTGCATTACCCAGACCTTGCGGCCCGCATCCATTCCCGTGCGCCCGAGCGTTATCTCTGGGACGT
GGCCGAGACCATCAAATACGGCTCGGGTTTTCCCAAGCTGATCAATGACGAAGAAATCGTGCCGCTTTACGTTTCCAAGG
GCGCGACCTTTGAAGAAGCGCTGGACTATGCCGTTTCCGGCTGCACCGAGGCGCGCATGCCCAATCGCGACACCTATACG
TCCGGCGGAGCCTACATCAACTTTGCCGCTGCCGTGGAAATGGTGTTGCGCAACGGGCGGATGAAAAAATACGGCGATCA
GAAACTCGGTGTTGAAACAGGCGACCCCCGCAGCTTTACGACCTGGGACCAGTTCTGGAATGCCTATGTTGAGCAGCACC
TGCTCTTCCTCAAGACCGCGTTTACCCAGCAGTACATCATCAATAAACTGCGTGCCGAACACTTTGCACAGCCGATGGGT
TCCGCCATGCATGACCTGTGCATGAAGCACTGCATTGACCTGCATCAGGAGCAGATTCCCGAAGGCATCAACCTCGGCTA
CTTTGAGTATATGGGGCTGGGAACCGTGGTAGATTCCCTGGCGGCCGTAAAAAAACTGGTTTTTGAAGAAAAGAAGCTGA
GCATGGACAAGCTCATTGCTGCCATCGACGCTGACTTTGAAGGCTATGAAGACGTGCGCGCCCTGTTGCGGTCCGCCCCC
TGTTACGGCAACAATGATGAGTACGCCGATGCCATTGGCCGCGACATTGACAGGATTTCGGTGGAATACGGCAACAAGTA
CTCCATGAGCGATCTGGGCATTCATAATGATGTGCGCTACGTCCCCTTCACCTCACATGTGCCTTTCGGCAAGGTGGTTT
CCGCCACGCCCAATGGTCGTACTGACGGTTTTCCCCTCTCCGATGGCTCTTCTGCCTCGCACGGGGCTGATGTGAATGGC
CCGACCGCAGTGCTGCTCTCAAACTGCACCACCAAAAATATGGGCCTGCGCGACCGTGCCGCCCGCATGCTGAACATCAA
GTTTACGCCCAAATGCGTGGAAGGCGAGCAGGGCACAGAAAAATTGGTGTCCTTCATCCGCACGTTCTGCGACCTCAAAC
TCTGGCATGTGCAGTTTAACGTGGTCAACAAGGGCACCCTTGTGGCTGCGCAGAAAGATCCGCAGAAATACCGGAACCTC
ATTGTGCGTATCGCCGGCTACAGCGCCTACTTTGTGGACCTGTCGCCCGATTTGCAGAACGATCTCATCGCCCGTACAGA
ACATGATGTGATGTAA

Upstream 100 bases:

>100_bases
GCCGCCATAACGAGGATAGCCAGCTTTACCAGGCGTTCTCAGCGCGGCGGCATGCAGGCCGTACCGCGCTCTGTAATCCA
TATTATTCAGGAGTAGCGTG

Downstream 100 bases:

>100_bases
GGACGGCATCTGAAGCGGGGAGGAGATTTCCTCCCCGCATTTTTACGACAGTTTTTTCTTGATACCGCCGGTTGCTATAT
AACGGCATTGCGCAAAAAAG

Product: Formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 831; Mature: 830

Protein sequence:

>831_residues
MSMTTCECRSPQEQRLYDKIEGREDRFRKTHPRVFRLLERFEGQKPRIDIERALYFTQSMQETEGQPLVLRWAKALMHIA
RNMTVYVQEDQLLLGRAGCDGRYGILYPELDGDFLDIAVRDLPTRKTSPATITPEDARRVVEEIAPYWKGKTYHEALNAA
LPAEVHKLTYDDPEGLISRFIVNETSSFRSSIQWVHDYEKILKRGFNSIKKEAREKLAALDPLSAKDDREKRPFLEAVMI
VCDAIVLWAKRHAVLAREMAEKESDPVRKAELLRMAENAEHVPGEPARDFWEACQSQWFTQMFSRIEQKTGTTISNGRMD
QYFQPYYKQDREAGKITEAQAMELLECMWVGMAEFIDMYISPTGGAFNEGYAHWEAVTVGGQTPDGRDASNDLTYLILKS
KREFPLHYPDLAARIHSRAPERYLWDVAETIKYGSGFPKLINDEEIVPLYVSKGATFEEALDYAVSGCTEARMPNRDTYT
SGGAYINFAAAVEMVLRNGRMKKYGDQKLGVETGDPRSFTTWDQFWNAYVEQHLLFLKTAFTQQYIINKLRAEHFAQPMG
SAMHDLCMKHCIDLHQEQIPEGINLGYFEYMGLGTVVDSLAAVKKLVFEEKKLSMDKLIAAIDADFEGYEDVRALLRSAP
CYGNNDEYADAIGRDIDRISVEYGNKYSMSDLGIHNDVRYVPFTSHVPFGKVVSATPNGRTDGFPLSDGSSASHGADVNG
PTAVLLSNCTTKNMGLRDRAARMLNIKFTPKCVEGEQGTEKLVSFIRTFCDLKLWHVQFNVVNKGTLVAAQKDPQKYRNL
IVRIAGYSAYFVDLSPDLQNDLIARTEHDVM

Sequences:

>Translated_831_residues
MSMTTCECRSPQEQRLYDKIEGREDRFRKTHPRVFRLLERFEGQKPRIDIERALYFTQSMQETEGQPLVLRWAKALMHIA
RNMTVYVQEDQLLLGRAGCDGRYGILYPELDGDFLDIAVRDLPTRKTSPATITPEDARRVVEEIAPYWKGKTYHEALNAA
LPAEVHKLTYDDPEGLISRFIVNETSSFRSSIQWVHDYEKILKRGFNSIKKEAREKLAALDPLSAKDDREKRPFLEAVMI
VCDAIVLWAKRHAVLAREMAEKESDPVRKAELLRMAENAEHVPGEPARDFWEACQSQWFTQMFSRIEQKTGTTISNGRMD
QYFQPYYKQDREAGKITEAQAMELLECMWVGMAEFIDMYISPTGGAFNEGYAHWEAVTVGGQTPDGRDASNDLTYLILKS
KREFPLHYPDLAARIHSRAPERYLWDVAETIKYGSGFPKLINDEEIVPLYVSKGATFEEALDYAVSGCTEARMPNRDTYT
SGGAYINFAAAVEMVLRNGRMKKYGDQKLGVETGDPRSFTTWDQFWNAYVEQHLLFLKTAFTQQYIINKLRAEHFAQPMG
SAMHDLCMKHCIDLHQEQIPEGINLGYFEYMGLGTVVDSLAAVKKLVFEEKKLSMDKLIAAIDADFEGYEDVRALLRSAP
CYGNNDEYADAIGRDIDRISVEYGNKYSMSDLGIHNDVRYVPFTSHVPFGKVVSATPNGRTDGFPLSDGSSASHGADVNG
PTAVLLSNCTTKNMGLRDRAARMLNIKFTPKCVEGEQGTEKLVSFIRTFCDLKLWHVQFNVVNKGTLVAAQKDPQKYRNL
IVRIAGYSAYFVDLSPDLQNDLIARTEHDVM
>Mature_830_residues
SMTTCECRSPQEQRLYDKIEGREDRFRKTHPRVFRLLERFEGQKPRIDIERALYFTQSMQETEGQPLVLRWAKALMHIAR
NMTVYVQEDQLLLGRAGCDGRYGILYPELDGDFLDIAVRDLPTRKTSPATITPEDARRVVEEIAPYWKGKTYHEALNAAL
PAEVHKLTYDDPEGLISRFIVNETSSFRSSIQWVHDYEKILKRGFNSIKKEAREKLAALDPLSAKDDREKRPFLEAVMIV
CDAIVLWAKRHAVLAREMAEKESDPVRKAELLRMAENAEHVPGEPARDFWEACQSQWFTQMFSRIEQKTGTTISNGRMDQ
YFQPYYKQDREAGKITEAQAMELLECMWVGMAEFIDMYISPTGGAFNEGYAHWEAVTVGGQTPDGRDASNDLTYLILKSK
REFPLHYPDLAARIHSRAPERYLWDVAETIKYGSGFPKLINDEEIVPLYVSKGATFEEALDYAVSGCTEARMPNRDTYTS
GGAYINFAAAVEMVLRNGRMKKYGDQKLGVETGDPRSFTTWDQFWNAYVEQHLLFLKTAFTQQYIINKLRAEHFAQPMGS
AMHDLCMKHCIDLHQEQIPEGINLGYFEYMGLGTVVDSLAAVKKLVFEEKKLSMDKLIAAIDADFEGYEDVRALLRSAPC
YGNNDEYADAIGRDIDRISVEYGNKYSMSDLGIHNDVRYVPFTSHVPFGKVVSATPNGRTDGFPLSDGSSASHGADVNGP
TAVLLSNCTTKNMGLRDRAARMLNIKFTPKCVEGEQGTEKLVSFIRTFCDLKLWHVQFNVVNKGTLVAAQKDPQKYRNLI
VRIAGYSAYFVDLSPDLQNDLIARTEHDVM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=800, Percent_Identity=31.625, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1790388, Length=806, Percent_Identity=32.5062034739454, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1787131, Length=643, Percent_Identity=23.4836702954899, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI48994926, Length=647, Percent_Identity=24.8840803709428, Blast_Score=113, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 94549; Mature: 94418

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTTCECRSPQEQRLYDKIEGREDRFRKTHPRVFRLLERFEGQKPRIDIERALYFTQSM
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QETEGQPLVLRWAKALMHIARNMTVYVQEDQLLLGRAGCDGRYGILYPELDGDFLDIAVR
HHCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEEEECCCCCEEEHHHH
DLPTRKTSPATITPEDARRVVEEIAPYWKGKTYHEALNAALPAEVHKLTYDDPEGLISRF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
IVNETSSFRSSIQWVHDYEKILKRGFNSIKKEAREKLAALDPLSAKDDREKRPFLEAVMI
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
VCDAIVLWAKRHAVLAREMAEKESDPVRKAELLRMAENAEHVPGEPARDFWEACQSQWFT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
QMFSRIEQKTGTTISNGRMDQYFQPYYKQDREAGKITEAQAMELLECMWVGMAEFIDMYI
HHHHHHHHHCCCCCCCCCCHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
SPTGGAFNEGYAHWEAVTVGGQTPDGRDASNDLTYLILKSKREFPLHYPDLAARIHSRAP
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC
ERYLWDVAETIKYGSGFPKLINDEEIVPLYVSKGATFEEALDYAVSGCTEARMPNRDTYT
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHCCCCCCCCCC
SGGAYINFAAAVEMVLRNGRMKKYGDQKLGVETGDPRSFTTWDQFWNAYVEQHLLFLKTA
CCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FTQQYIINKLRAEHFAQPMGSAMHDLCMKHCIDLHQEQIPEGINLGYFEYMGLGTVVDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AAVKKLVFEEKKLSMDKLIAAIDADFEGYEDVRALLRSAPCYGNNDEYADAIGRDIDRIS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHEE
VEYGNKYSMSDLGIHNDVRYVPFTSHVPFGKVVSATPNGRTDGFPLSDGSSASHGADVNG
HCCCCEECCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PTAVLLSNCTTKNMGLRDRAARMLNIKFTPKCVEGEQGTEKLVSFIRTFCDLKLWHVQFN
CCEEEEECCCCCCCCCHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEE
VVNKGTLVAAQKDPQKYRNLIVRIAGYSAYFVDLSPDLQNDLIARTEHDVM
EECCCEEEEECCCHHHHHHHHHHEECCEEEEEECCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SMTTCECRSPQEQRLYDKIEGREDRFRKTHPRVFRLLERFEGQKPRIDIERALYFTQSM
CCCCCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QETEGQPLVLRWAKALMHIARNMTVYVQEDQLLLGRAGCDGRYGILYPELDGDFLDIAVR
HHCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEEEECCCCCEEEHHHH
DLPTRKTSPATITPEDARRVVEEIAPYWKGKTYHEALNAALPAEVHKLTYDDPEGLISRF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
IVNETSSFRSSIQWVHDYEKILKRGFNSIKKEAREKLAALDPLSAKDDREKRPFLEAVMI
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
VCDAIVLWAKRHAVLAREMAEKESDPVRKAELLRMAENAEHVPGEPARDFWEACQSQWFT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
QMFSRIEQKTGTTISNGRMDQYFQPYYKQDREAGKITEAQAMELLECMWVGMAEFIDMYI
HHHHHHHHHCCCCCCCCCCHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
SPTGGAFNEGYAHWEAVTVGGQTPDGRDASNDLTYLILKSKREFPLHYPDLAARIHSRAP
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC
ERYLWDVAETIKYGSGFPKLINDEEIVPLYVSKGATFEEALDYAVSGCTEARMPNRDTYT
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHCCCCCCCCCC
SGGAYINFAAAVEMVLRNGRMKKYGDQKLGVETGDPRSFTTWDQFWNAYVEQHLLFLKTA
CCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FTQQYIINKLRAEHFAQPMGSAMHDLCMKHCIDLHQEQIPEGINLGYFEYMGLGTVVDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AAVKKLVFEEKKLSMDKLIAAIDADFEGYEDVRALLRSAPCYGNNDEYADAIGRDIDRIS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHEE
VEYGNKYSMSDLGIHNDVRYVPFTSHVPFGKVVSATPNGRTDGFPLSDGSSASHGADVNG
HCCCCEECCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PTAVLLSNCTTKNMGLRDRAARMLNIKFTPKCVEGEQGTEKLVSFIRTFCDLKLWHVQFN
CCEEEEECCCCCCCCCHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEE
VVNKGTLVAAQKDPQKYRNLIVRIAGYSAYFVDLSPDLQNDLIARTEHDVM
EECCCEEEEECCCHHHHHHHHHHEECCEEEEEECCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]