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The map label for this gene is dhbE [H]

Identifier: 220904606

GI number: 220904606

Start: 1605018

End: 1606688

Strand: Reverse

Name: dhbE [H]

Synonym: Ddes_1338

Alternate gene names: 220904606

Gene position: 1606688-1605018 (Counterclockwise)

Preceding gene: 220904607

Following gene: 220904605

Centisome position: 55.92

GC content: 53.98

Gene sequence:

>1671_bases
ATGCTTTATGCGCGTGATTTGGGGTGGCCACAGGATATTATTAACAAATACCGTGCGGAAGATTATTGGCGCGATGAAAC
GTTCAATACCCTTCTGCAGGATGTTGCCGGGCGCAAGGGAGACTGCATGGCAGTTGTCGATGGCAACGTTCGCCTCACCT
ATGCAGAATTCAAGGAAATGGTTGACAGAATTGCGGGAGGTTTTGCCGCTCTTGGGCTCGGGGCCGGCGACCACGTTGTG
GTGCAGCTTCCCAACTGCTGGCAGTTTCTTGTTGTTTTTTTCGGCCTGTCACGGCTGGGAGTCGCTCCGGTTCTGGCCCT
GCCTGCGCACAGGCTATCAGAAATTTCCTCCTTCACAGGGGTAACTGAGGCAAAAGCCTATATTTGCGCTGCCAGGTCGG
CCGGTTTTGATTACCGGCAGCTGGCGCGTGAACTGCAGGAAAGGCACCCGGGCCTGAAGCACGTAATCGTGAGCGGCGAG
CCGGAAGAATTCATTTCCCTTGAAGGTCTGTTGGCGTCATCGACCGATCTTCCGCAAGATCCTGTTTCGTCAGATTCACT
GCTCTGTTTTCTTCTTTCTGGCGGCACGACCAGCGTTCCCAAGCTGATTGCCCGCGTTCATTGCGAATATTCGTGCGTCA
CCCGTTACAGTGCCGCCGCCAACGGTTTTGATGAAACCACGGTATATCTGGCTGCACTGCCAATGGCACACAACTTTCCG
CTCGGCGCTCCCGGTGTGCTGGGAACGCTTCAATGCGGCGGTACCGTGGTCATCGCCGAAACCCCTGAACCGGATATGTG
CTTTGACCTTATTGACAGGAACATGGTTACCCATACCGCTCTTGTGCCGCCCGCAGCTATACTCTGGTGCGACATGGCCG
AATTGTTGGAGCGCCAGAATACCTTTCCAAGTTTGAGCAACATCCAGGTTGGTGGTTCACGGGTTGGCGAAGACCTTGCC
CAACGCACTACAGAAATCTTTGGCTGCCGCTTTCAGAATGTTTTTGGCATGTCAGAAGGTCTGATCAGTATGACCCGTAT
GGATATGGATGAGGAAATAGTCCGCAATACGCAGGGATTGCCCGTGTGCCCGGCTGACGAGTTTCGCATCGTTGATCCTG
CTGGCCGTGAAGTGCCTGATGGAGTCGTGGGTGGCCTGCAGATTCGTGGACCGTACACCATACATACTTACTTCAAACGG
CCCGAAGCCAATGCGGAATCATTCACCGGGGACGGATTCTTTTGTACCGGGGACCTTGCGCGGCGCAGGTCTGATGGTTG
CCTGGTGCTTGAAGGACGTGAAAAAGATCAGATCCAGCGGGGAGGCGAAAAGATCATTCCCGAAGAAGTGGAAAGTGTTC
TTGTCGCCCACAGGGAAGTCCGGGATGCGGTTCTTCTGGGCATTCCCGACAAGCTGCTTGGCGAAAAGATCTGCGCCTTC
ATCTTTGTGCATGAACACAGGGATGAAGAAGAGGTCAATGACCGCGCCCTCCGGCGCTTCCTCCAGAGTGAAGGGCTGAG
TACGTTCAAGATTCCTGATCAGTTTATTTTTGTTACGTCTTTTCCAAGTACGGCAGTGGGCAAAAATAACCGCCGCAAAC
TGAGGGAGCAGCTTCTTCAGCAATACTACCGGAGCGTCAGTGTTCCTGCAGGAGTGGAAAATGCCAGTTGA

Upstream 100 bases:

>100_bases
CAGTCACGCGCCGTCAGGGGGTGAGCTTTCACCAGCCTGCCCTGTTCAACCATGGGGTGATTCGACTGCACGGCGCGTCG
ATTGGCAAAGAGGTACGGTT

Downstream 100 bases:

>100_bases
AGCGCCAATTACCTCGCGCGAAGGCCTGCGTGCCTGGGTGGCAGCCGCGCTCAATTGCTCTCCGGAGGCAATCGACGAGA
ATGATTCGCTTATCGAACTG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MLYARDLGWPQDIINKYRAEDYWRDETFNTLLQDVAGRKGDCMAVVDGNVRLTYAEFKEMVDRIAGGFAALGLGAGDHVV
VQLPNCWQFLVVFFGLSRLGVAPVLALPAHRLSEISSFTGVTEAKAYICAARSAGFDYRQLARELQERHPGLKHVIVSGE
PEEFISLEGLLASSTDLPQDPVSSDSLLCFLLSGGTTSVPKLIARVHCEYSCVTRYSAAANGFDETTVYLAALPMAHNFP
LGAPGVLGTLQCGGTVVIAETPEPDMCFDLIDRNMVTHTALVPPAAILWCDMAELLERQNTFPSLSNIQVGGSRVGEDLA
QRTTEIFGCRFQNVFGMSEGLISMTRMDMDEEIVRNTQGLPVCPADEFRIVDPAGREVPDGVVGGLQIRGPYTIHTYFKR
PEANAESFTGDGFFCTGDLARRRSDGCLVLEGREKDQIQRGGEKIIPEEVESVLVAHREVRDAVLLGIPDKLLGEKICAF
IFVHEHRDEEEVNDRALRRFLQSEGLSTFKIPDQFIFVTSFPSTAVGKNNRRKLREQLLQQYYRSVSVPAGVENAS

Sequences:

>Translated_556_residues
MLYARDLGWPQDIINKYRAEDYWRDETFNTLLQDVAGRKGDCMAVVDGNVRLTYAEFKEMVDRIAGGFAALGLGAGDHVV
VQLPNCWQFLVVFFGLSRLGVAPVLALPAHRLSEISSFTGVTEAKAYICAARSAGFDYRQLARELQERHPGLKHVIVSGE
PEEFISLEGLLASSTDLPQDPVSSDSLLCFLLSGGTTSVPKLIARVHCEYSCVTRYSAAANGFDETTVYLAALPMAHNFP
LGAPGVLGTLQCGGTVVIAETPEPDMCFDLIDRNMVTHTALVPPAAILWCDMAELLERQNTFPSLSNIQVGGSRVGEDLA
QRTTEIFGCRFQNVFGMSEGLISMTRMDMDEEIVRNTQGLPVCPADEFRIVDPAGREVPDGVVGGLQIRGPYTIHTYFKR
PEANAESFTGDGFFCTGDLARRRSDGCLVLEGREKDQIQRGGEKIIPEEVESVLVAHREVRDAVLLGIPDKLLGEKICAF
IFVHEHRDEEEVNDRALRRFLQSEGLSTFKIPDQFIFVTSFPSTAVGKNNRRKLREQLLQQYYRSVSVPAGVENAS
>Mature_556_residues
MLYARDLGWPQDIINKYRAEDYWRDETFNTLLQDVAGRKGDCMAVVDGNVRLTYAEFKEMVDRIAGGFAALGLGAGDHVV
VQLPNCWQFLVVFFGLSRLGVAPVLALPAHRLSEISSFTGVTEAKAYICAARSAGFDYRQLARELQERHPGLKHVIVSGE
PEEFISLEGLLASSTDLPQDPVSSDSLLCFLLSGGTTSVPKLIARVHCEYSCVTRYSAAANGFDETTVYLAALPMAHNFP
LGAPGVLGTLQCGGTVVIAETPEPDMCFDLIDRNMVTHTALVPPAAILWCDMAELLERQNTFPSLSNIQVGGSRVGEDLA
QRTTEIFGCRFQNVFGMSEGLISMTRMDMDEEIVRNTQGLPVCPADEFRIVDPAGREVPDGVVGGLQIRGPYTIHTYFKR
PEANAESFTGDGFFCTGDLARRRSDGCLVLEGREKDQIQRGGEKIIPEEVESVLVAHREVRDAVLLGIPDKLLGEKICAF
IFVHEHRDEEEVNDRALRRFLQSEGLSTFKIPDQFIFVTSFPSTAVGKNNRRKLREQLLQQYYRSVSVPAGVENAS

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI115511026, Length=564, Percent_Identity=25, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI156151445, Length=178, Percent_Identity=35.9550561797753, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI38505220, Length=518, Percent_Identity=24.1312741312741, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI157311624, Length=539, Percent_Identity=23.3766233766234, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI157311622, Length=539, Percent_Identity=23.3766233766234, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI187761345, Length=382, Percent_Identity=23.2984293193717, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI187761343, Length=382, Percent_Identity=23.2984293193717, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI58082049, Length=551, Percent_Identity=23.7749546279492, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI28416953, Length=540, Percent_Identity=21.1111111111111, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI122937307, Length=548, Percent_Identity=23.1751824817518, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI42544132, Length=522, Percent_Identity=20.6896551724138, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1786810, Length=530, Percent_Identity=39.2452830188679, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI145693145, Length=541, Percent_Identity=25.1386321626617, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI221142682, Length=541, Percent_Identity=26.0628465804067, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788107, Length=567, Percent_Identity=24.8677248677249, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1786801, Length=466, Percent_Identity=23.6051502145923, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790505, Length=531, Percent_Identity=22.5988700564972, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1786529, Length=524, Percent_Identity=21.1832061068702, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538037, Length=536, Percent_Identity=26.1194029850746, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=560, Percent_Identity=25.3571428571429, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=535, Percent_Identity=26.5420560747664, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71994703, Length=525, Percent_Identity=25.1428571428571, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=525, Percent_Identity=25.1428571428571, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=525, Percent_Identity=25.1428571428571, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17559526, Length=554, Percent_Identity=23.826714801444, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=479, Percent_Identity=24.6346555323591, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=514, Percent_Identity=21.9844357976654, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=516, Percent_Identity=22.093023255814, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115534440, Length=550, Percent_Identity=23.0909090909091, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=511, Percent_Identity=22.1135029354207, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=565, Percent_Identity=22.8318584070796, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=533, Percent_Identity=23.0769230769231, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17507435, Length=528, Percent_Identity=24.0530303030303, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=547, Percent_Identity=26.508226691042, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323182, Length=545, Percent_Identity=24.0366972477064, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24581924, Length=552, Percent_Identity=25.5434782608696, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI18859661, Length=550, Percent_Identity=24, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922652, Length=536, Percent_Identity=23.6940298507463, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24656500, Length=505, Percent_Identity=22.7722772277228, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356947, Length=514, Percent_Identity=21.2062256809339, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI20130357, Length=502, Percent_Identity=24.1035856573705, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24667955, Length=315, Percent_Identity=27.9365079365079, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24667959, Length=312, Percent_Identity=27.5641025641026, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI62472339, Length=312, Percent_Identity=27.5641025641026, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355181, Length=520, Percent_Identity=20.9615384615385, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.-

Molecular weight: Translated: 61340; Mature: 61340

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYARDLGWPQDIINKYRAEDYWRDETFNTLLQDVAGRKGDCMAVVDGNVRLTYAEFKEM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHH
VDRIAGGFAALGLGAGDHVVVQLPNCWQFLVVFFGLSRLGVAPVLALPAHRLSEISSFTG
HHHHHCCHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCC
VTEAKAYICAARSAGFDYRQLARELQERHPGLKHVIVSGEPEEFISLEGLLASSTDLPQD
CHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCC
PVSSDSLLCFLLSGGTTSVPKLIARVHCEYSCVTRYSAAANGFDETTVYLAALPMAHNFP
CCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHEEEEEEECCCCCCC
LGAPGVLGTLQCGGTVVIAETPEPDMCFDLIDRNMVTHTALVPPAAILWCDMAELLERQN
CCCCCCEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCC
TFPSLSNIQVGGSRVGEDLAQRTTEIFGCRFQNVFGMSEGLISMTRMDMDEEIVRNTQGL
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHCCCCC
PVCPADEFRIVDPAGREVPDGVVGGLQIRGPYTIHTYFKRPEANAESFTGDGFFCTGDLA
CCCCCCCEEEECCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCCEEEECHHH
RRRSDGCLVLEGREKDQIQRGGEKIIPEEVESVLVAHREVRDAVLLGIPDKLLGEKICAF
HCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHH
IFVHEHRDEEEVNDRALRRFLQSEGLSTFKIPDQFIFVTSFPSTAVGKNNRRKLREQLLQ
EEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCHHCCCCHHHHHHHHHHH
QYYRSVSVPAGVENAS
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLYARDLGWPQDIINKYRAEDYWRDETFNTLLQDVAGRKGDCMAVVDGNVRLTYAEFKEM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHH
VDRIAGGFAALGLGAGDHVVVQLPNCWQFLVVFFGLSRLGVAPVLALPAHRLSEISSFTG
HHHHHCCHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCC
VTEAKAYICAARSAGFDYRQLARELQERHPGLKHVIVSGEPEEFISLEGLLASSTDLPQD
CHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCC
PVSSDSLLCFLLSGGTTSVPKLIARVHCEYSCVTRYSAAANGFDETTVYLAALPMAHNFP
CCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHEEEEEEECCCCCCC
LGAPGVLGTLQCGGTVVIAETPEPDMCFDLIDRNMVTHTALVPPAAILWCDMAELLERQN
CCCCCCEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCC
TFPSLSNIQVGGSRVGEDLAQRTTEIFGCRFQNVFGMSEGLISMTRMDMDEEIVRNTQGL
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHCCCCC
PVCPADEFRIVDPAGREVPDGVVGGLQIRGPYTIHTYFKRPEANAESFTGDGFFCTGDLA
CCCCCCCEEEECCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCCEEEECHHH
RRRSDGCLVLEGREKDQIQRGGEKIIPEEVESVLVAHREVRDAVLLGIPDKLLGEKICAF
HCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHH
IFVHEHRDEEEVNDRALRRFLQSEGLSTFKIPDQFIFVTSFPSTAVGKNNRRKLREQLLQ
EEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCHHCCCCHHHHHHHHHHH
QYYRSVSVPAGVENAS
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]