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The map label for this gene is appB [H]

Identifier: 220904484

GI number: 220904484

Start: 1427385

End: 1428419

Strand: Direct

Name: appB [H]

Synonym: Ddes_1214

Alternate gene names: 220904484

Gene position: 1427385-1428419 (Clockwise)

Preceding gene: 220904483

Following gene: 220904485

Centisome position: 49.68

GC content: 56.23

Gene sequence:

>1035_bases
ATGCCTCCCCTGCCCTCACATGCCCGCCGTCACGGTCGGCTGACCGCTATCGCTGCCAACGTCGCACGCAAGACGTTGTG
GATGTTGCTGGTCCTGTGGGGGATTACGCTTATCAGCTTCTGGGTCATTCATCTGGCTCCAGGGTCTCCTACGGATATGG
AGACAACGCTCAACCCCCTCGCAGGCGAGGCTGCCCGGCAAAGGCTTGAAGCGCTGTACGGACTTGACCGCCCGCTTCAC
GTCCAATATGCAGACTGGCTGCTGCGCTTGCTGCATTTTGACTTCGGCAACTCCATGTCTGCTGATTCACGACCCGTACT
GACCAAGATTATGGAGCGCCTGCCCCTCACAGTGGGCATGAATGTCACCGCCATGGTGCTGACTCTGCTCATTGCCATAC
CGGTGGGAATAATTTCGGCCTGCCGCCGCAACTCTCTGCTGGACCGCTCTGTGACCGTGCTGGTTTTTCTGGGCTTTGCC
ATGCCTTCATTCTGGCTTGCGTTGCTGCTCATGTTGCTGTTTGGCATTGAGTTGCAATGGTTACCCATCTCCGGCCTTAC
ATCCATGCACTATGAGCAGCTGAGCCTATGGGGAAAGTTTTGTGATCTGGCAAAACATCTGGCTCTGCCCATACTTGTCT
ATACCGTGGGCGGTCTTGCGGGCATGTCACGCTATATGCGGGCGTGCATGTTGGAGGTTCTTCAACAGGATTACATCCTT
ACGGCACGGGCCAAAGGCCTTGGCGAAAAGGCCATTATCTGGCGTCACGCGCTGCGCAATGCGCTATTGCCCGTCATTAC
CCTGCTGGGACTTTCAGTGCCTGGACTCATCGGCGGCAGCGTCATTATTGAGTCCATTTTTGCCCTGCCCGGCCTCGGTC
AGCTCTTTTATGCAGCTGTTATGGCCCGGGATTACACTATGATTATGGGCAACCTTGTTTTGGGGGCCGTGCTTACCCTC
TTTGGCAATCTGCTGGCCGACATCTGCTATGGCCTGGCTGACCCGCGTATACGCAGTGCAAAGGATAACGCCTGA

Upstream 100 bases:

>100_bases
CTGTACGCGCGCTTTCTTGCTCTGCGTCTTTCTCCACAGTGGCAGAAAGAAGTGGACGACATGCGCCAGTGGATGACCCG
TACCGAGTAGAGAGCCGCTC

Downstream 100 bases:

>100_bases
TGCTTCCCCGCGCTGTCAAAAATGCGCTTGGGCGTCATGCCATGCTGCTGATCGGGCTTTTTATTGTCCTTGGCATGTCC
CTGGCCGCCCTGCTGGCTCC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MPPLPSHARRHGRLTAIAANVARKTLWMLLVLWGITLISFWVIHLAPGSPTDMETTLNPLAGEAARQRLEALYGLDRPLH
VQYADWLLRLLHFDFGNSMSADSRPVLTKIMERLPLTVGMNVTAMVLTLLIAIPVGIISACRRNSLLDRSVTVLVFLGFA
MPSFWLALLLMLLFGIELQWLPISGLTSMHYEQLSLWGKFCDLAKHLALPILVYTVGGLAGMSRYMRACMLEVLQQDYIL
TARAKGLGEKAIIWRHALRNALLPVITLLGLSVPGLIGGSVIIESIFALPGLGQLFYAAVMARDYTMIMGNLVLGAVLTL
FGNLLADICYGLADPRIRSAKDNA

Sequences:

>Translated_344_residues
MPPLPSHARRHGRLTAIAANVARKTLWMLLVLWGITLISFWVIHLAPGSPTDMETTLNPLAGEAARQRLEALYGLDRPLH
VQYADWLLRLLHFDFGNSMSADSRPVLTKIMERLPLTVGMNVTAMVLTLLIAIPVGIISACRRNSLLDRSVTVLVFLGFA
MPSFWLALLLMLLFGIELQWLPISGLTSMHYEQLSLWGKFCDLAKHLALPILVYTVGGLAGMSRYMRACMLEVLQQDYIL
TARAKGLGEKAIIWRHALRNALLPVITLLGLSVPGLIGGSVIIESIFALPGLGQLFYAAVMARDYTMIMGNLVLGAVLTL
FGNLLADICYGLADPRIRSAKDNA
>Mature_343_residues
PPLPSHARRHGRLTAIAANVARKTLWMLLVLWGITLISFWVIHLAPGSPTDMETTLNPLAGEAARQRLEALYGLDRPLHV
QYADWLLRLLHFDFGNSMSADSRPVLTKIMERLPLTVGMNVTAMVLTLLIAIPVGIISACRRNSLLDRSVTVLVFLGFAM
PSFWLALLLMLLFGIELQWLPISGLTSMHYEQLSLWGKFCDLAKHLALPILVYTVGGLAGMSRYMRACMLEVLQQDYILT
ARAKGLGEKAIIWRHALRNALLPVITLLGLSVPGLIGGSVIIESIFALPGLGQLFYAAVMARDYTMIMGNLVLGAVLTLF
GNLLADICYGLADPRIRSAKDNA

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=324, Percent_Identity=37.037037037037, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1787497, Length=318, Percent_Identity=35.5345911949685, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789888, Length=324, Percent_Identity=38.2716049382716, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1788504, Length=360, Percent_Identity=31.3888888888889, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1787053, Length=318, Percent_Identity=33.3333333333333, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1787761, Length=333, Percent_Identity=36.036036036036, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1787550, Length=266, Percent_Identity=32.3308270676692, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37854; Mature: 37723

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PPLPSHARRHGRLTAIAANVARKTLWMLLVLWGITLISFWVIHLAPGSPTDMETTLNPL
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
AGEAARQRLEALYGLDRPLHVQYADWLLRLLHFDFGNSMSADSRPVLTKIMERLPLTVGM
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCC
NVTAMVLTLLIAIPVGIISACRRNSLLDRSVTVLVFLGFAMPSFWLALLLMLLFGIELQW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LPISGLTSMHYEQLSLWGKFCDLAKHLALPILVYTVGGLAGMSRYMRACMLEVLQQDYIL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TARAKGLGEKAIIWRHALRNALLPVITLLGLSVPGLIGGSVIIESIFALPGLGQLFYAAV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
MARDYTMIMGNLVLGAVLTLFGNLLADICYGLADPRIRSAKDNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]