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The map label for this gene is pcm

Identifier: 220904460

GI number: 220904460

Start: 1403072

End: 1403713

Strand: Direct

Name: pcm

Synonym: Ddes_1190

Alternate gene names: 220904460

Gene position: 1403072-1403713 (Clockwise)

Preceding gene: 220904457

Following gene: 220904461

Centisome position: 48.83

GC content: 63.08

Gene sequence:

>642_bases
ATGGTGGACCCCAAACGCCTGCGACGACGCATGGTGCGTGAACAACTTGAAGCCAGAGGCATAAACGATGCCGAGGTGCT
GGCCGCCATGTCGGCCGTGCCGCGTCATCTGTTTGTGCAGGAGGCGCTGCGCGCCCAGGCGTATGAGGATACCCCGCTGC
CCATCGGCTACGGTCAGACCATTTCACAGCCTTATGTGGTGGCACTGATGAGCCAGTTGCTTGAAGTGCGCCGCGGCATG
CGCGTGCTGGAAATAGGCACTGGCTCGGGATACCAGGCCGCGGTGCTCGCGACTATGGGCTGCACTGTCTTTACAGTGGA
GCGTTTGCGCGAACTGTACCAGGATACAGGAAATCTCTTGCGGCAACTGGGCCTGCGCGGCGTACACATGCAGCGTCGGG
ACGGCACGCTGGGCATGCCCGAGGCCGCCCCCTTTGACAGAATCATTGTTACGGCGGGCGGGCCGGAAGTGCCGCGCCCC
CTGACAGACCAGCTTGACGAAGGCGGCATTCTGCTTATTCCCGTGGGACCACGCCCCCGAGCACAGCGGCTCATGCGTTT
TCGCAAGGAGCAGGGCCGCATGACCGGGGAAGACCTCGGTCCCGCTATTTTTGTGGACCTGGTGGGCGACCACGGCTGGT
AG

Upstream 100 bases:

>100_bases
AGAGCGCATTGACTTGACGGGCAAAATCTGTTTTCACTTCCGCAGTATGCAACACGCCAAGTACACAGGCCAAAGAGCGC
CGGAACGCACACGTAGCCCC

Downstream 100 bases:

>100_bases
CACGGCCTCTCGCCATCAAAAACAAGGAGATGTCATGGCTTCCTGTTCCTCCTGTTCTTCATCTTCCTCCTGTCCAAGCG
CCACGGGCAAGGGTGGAGAC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MVDPKRLRRRMVREQLEARGINDAEVLAAMSAVPRHLFVQEALRAQAYEDTPLPIGYGQTISQPYVVALMSQLLEVRRGM
RVLEIGTGSGYQAAVLATMGCTVFTVERLRELYQDTGNLLRQLGLRGVHMQRRDGTLGMPEAAPFDRIIVTAGGPEVPRP
LTDQLDEGGILLIPVGPRPRAQRLMRFRKEQGRMTGEDLGPAIFVDLVGDHGW

Sequences:

>Translated_213_residues
MVDPKRLRRRMVREQLEARGINDAEVLAAMSAVPRHLFVQEALRAQAYEDTPLPIGYGQTISQPYVVALMSQLLEVRRGM
RVLEIGTGSGYQAAVLATMGCTVFTVERLRELYQDTGNLLRQLGLRGVHMQRRDGTLGMPEAAPFDRIIVTAGGPEVPRP
LTDQLDEGGILLIPVGPRPRAQRLMRFRKEQGRMTGEDLGPAIFVDLVGDHGW
>Mature_213_residues
MVDPKRLRRRMVREQLEARGINDAEVLAAMSAVPRHLFVQEALRAQAYEDTPLPIGYGQTISQPYVVALMSQLLEVRRGM
RVLEIGTGSGYQAAVLATMGCTVFTVERLRELYQDTGNLLRQLGLRGVHMQRRDGTLGMPEAAPFDRIIVTAGGPEVPRP
LTDQLDEGGILLIPVGPRPRAQRLMRFRKEQGRMTGEDLGPAIFVDLVGDHGW

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=198, Percent_Identity=35.3535353535354, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1789100, Length=195, Percent_Identity=49.2307692307692, Blast_Score=167, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI71983477, Length=190, Percent_Identity=33.6842105263158, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=188, Percent_Identity=32.9787234042553, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17981723, Length=201, Percent_Identity=29.8507462686567, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_DESDA (B8J017)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479772.1
- ProteinModelPortal:   B8J017
- GeneID:   7284872
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1190
- HOGENOM:   HBG699907
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23684; Mature: 23684

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDPKRLRRRMVREQLEARGINDAEVLAAMSAVPRHLFVQEALRAQAYEDTPLPIGYGQT
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ISQPYVVALMSQLLEVRRGMRVLEIGTGSGYQAAVLATMGCTVFTVERLRELYQDTGNLL
CCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQLGLRGVHMQRRDGTLGMPEAAPFDRIIVTAGGPEVPRPLTDQLDEGGILLIPVGPRPR
HHHCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEEECCCCHH
AQRLMRFRKEQGRMTGEDLGPAIFVDLVGDHGW
HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MVDPKRLRRRMVREQLEARGINDAEVLAAMSAVPRHLFVQEALRAQAYEDTPLPIGYGQT
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ISQPYVVALMSQLLEVRRGMRVLEIGTGSGYQAAVLATMGCTVFTVERLRELYQDTGNLL
CCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQLGLRGVHMQRRDGTLGMPEAAPFDRIIVTAGGPEVPRPLTDQLDEGGILLIPVGPRPR
HHHCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEEECCCCHH
AQRLMRFRKEQGRMTGEDLGPAIFVDLVGDHGW
HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA