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The map label for this gene is folD [H]

Identifier: 218905317

GI number: 218905317

Start: 4011939

End: 4012799

Strand: Reverse

Name: folD [H]

Synonym: BCAH820_4201

Alternate gene names: 218905317

Gene position: 4012799-4011939 (Counterclockwise)

Preceding gene: 218905318

Following gene: 218905316

Centisome position: 75.67

GC content: 37.86

Gene sequence:

>861_bases
ATGGTAGCAGTAATCATCAAAGGAAATGAAGTTGCGGAGAAAAAACGAGCACAATTAAAAGAAGAAGTTGTGAAGTTAAA
AGAGCAAGGGATTGTACCAGGATTAGCAGTTATTTTAGTTGGAGAAGATCCAGCATCTCGTTCTTATGTAAAAGGAAAAG
AAAAGGGCTGTGAACAAGTAGGAATCTATTCAGAGCTAATTGAATTTCCTGAAACAATTACGGAGGAGCGTTTGCTTGCT
GAAATCGATCGCTTAAATGGCGATGACCGCATTAATGGCATATTAGTGCAATTACCTTTACCAAAACATATTGAAGAAAA
AGCTATCATTGAAAGAATTTCACCAGAAAAAGATGTAGATGGATTTCACCCAATCAGCGTGGGACGTATGATGACGGGAC
AAGATACATTCCTTCCATGTACACCGCATGGTATTGTAGAGTTAGTAAAAGAAACAAATCTTGATATCTCAGGAAAACAT
GTTGTAGTAATTGGAAGAAGTAATATTGTCGGCAAGCCAGTGGGACAATTGTTCTTAAATGAAAATGCAACTGTCACATA
CTGTCATTCTAAGACGCAAAACATGAAAGAGTTAACGAAGTTAGCTGATATTTTAATCGTAGCAGTAGGACGACCTAAAA
TGGTAACAGCTGATTATATCAAAGAAGGAGCAGTTGTAATCGACGTTGGTGTAAACCGTTTAGAAACAGGAAAACTTTGT
GGTGATGTTGACTTTGACAACGTGTTAGATGTTGCAGGTTACATTACACCTGTACCAAAGGGTGTTGGTCCAATGACAAT
CACGATGCTTCTTCATAATACTGTTGAATCGGCTAAGCGTGCAGGTGTCGTTTGTAAATAA

Upstream 100 bases:

>100_bases
TTGCTAAAACATTTGGGGATGAGGAATCTCGTCGTTTTATTAACGGCGTTTTATCTAATATAAAAGATACACTGTAATTT
CTTTAAGGGGGAATCTTAAA

Downstream 100 bases:

>100_bases
TTTGAGTCGGCTATGAGGAGAGAGAAATGGAGAAACAATATTTAACCGTTACAGCGTTAACACGCTATATAAAAACAAAA
ATAGAATATGATCCGCATTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQVGIYSELIEFPETITEERLLA
EIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVDGFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKH
VVVIGRSNIVGKPVGQLFLNENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
GDVDFDNVLDVAGYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK

Sequences:

>Translated_286_residues
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQVGIYSELIEFPETITEERLLA
EIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVDGFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKH
VVVIGRSNIVGKPVGQLFLNENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
GDVDFDNVLDVAGYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK
>Mature_286_residues
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQVGIYSELIEFPETITEERLLA
EIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVDGFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKH
VVVIGRSNIVGKPVGQLFLNENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
GDVDFDNVLDVAGYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=49.6598639455782, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=46.8965517241379, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=47.2972972972973, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI36796743, Length=229, Percent_Identity=23.5807860262009, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=50, Blast_Score=275, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=46.5753424657534, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=45.3287197231834, Blast_Score=248, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=41.9463087248322, Blast_Score=232, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6322933, Length=319, Percent_Identity=27.5862068965517, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=46.3667820069204, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=46.3667820069204, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=46.3667820069204, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=46.3667820069204, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=43.3898305084746, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=43.3898305084746, Blast_Score=234, Evalue=6e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31152; Mature: 31152

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQV
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHCCHHHCHHHH
GIYSELIEFPETITEERLLAEIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKHVVVIGRSNIVGKPVGQLFLN
CCCCCCCCCEECCCCCEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEEC
ENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
CCCEEEEECCCCCCHHHHHHHHHHEEEECCCCCEEEHHHHHCCCEEEEECCCEECCCCCC
GDVDFDNVLDVAGYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYVKGKEKGCEQV
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHCCHHHCHHHH
GIYSELIEFPETITEERLLAEIDRLNGDDRINGILVQLPLPKHIEEKAIIERISPEKDVD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPISVGRMMTGQDTFLPCTPHGIVELVKETNLDISGKHVVVIGRSNIVGKPVGQLFLN
CCCCCCCCCEECCCCCEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEEC
ENATVTYCHSKTQNMKELTKLADILIVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
CCCEEEEECCCCCCHHHHHHHHHHEEEECCCCCEEEHHHHHCCCEEEEECCCEECCCCCC
GDVDFDNVLDVAGYITPVPKGVGPMTITMLLHNTVESAKRAGVVCK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA