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The map label for this gene is ykwC [H]

Identifier: 218905179

GI number: 218905179

Start: 3888321

End: 3889202

Strand: Reverse

Name: ykwC [H]

Synonym: BCAH820_4063

Alternate gene names: 218905179

Gene position: 3889202-3888321 (Counterclockwise)

Preceding gene: 218905180

Following gene: 218905178

Centisome position: 73.34

GC content: 36.85

Gene sequence:

>882_bases
ATGGAACATAAAACTTTATCAATAGGTTTCATCGGTATTGGTGTAATGGGAAAAAGTATGGTTCATCATTTAATGCAAGA
AGGTCATAAGGTATATGTATATAATAGAACGAAAGCGAAAACAGATTCTTTAGTGCAAGATGGTGCAAATTGGTGTGATA
CACCGAAAGACTTAGTAAAGCAAGTGGATGTTGTAATGACTATGGTAGGATATCCACATGATGTAGAAGAAGTGTATTTT
GGGATAGATGGGATTTTGGAGAATGCAAATGAAGGTACAATTACAATTGATTTCACGACATCTACACCAACATTGGCAAA
ACGTATTAATGAAGCGGGTAAAAGTAAGAATATATATACGTTAGATGCGCCTGTATCTGGAGGAGACGTCGGTGCGAAAG
AGGCAAGACTCGCAATTATGGTCGGTGGCGAGAAAGAAATATATGAAAAATGTTTACCGTTATTTGAAAAACTAGGAACA
AATATTCAGTTGCAAGGACCAGCTGGAAGTGGACAACATACAAAAATGTGCAATCAAATTGCAATTGCTTCTAATATGAT
TGGAGTATGTGAAGCGGTTGCTTATGCGAAAAAAGCTGGACTAGATCCAGATAAAGTATTAGAGAGTATTTCAACGGGGG
CAGCAGGTAGCTGGTCATTGAGTAATTTAGCTCCTCGCATGTTAAAGGGAGATTTTGAACCGGGGTTTTATGTAAAGCAT
TTTATGAAAGATATGAAGATTGCTTTAGATGAGGCTGAAAAATTACAATTACCAGTACCAGGTTTAAGTTTGGCGAAGGA
ATTGTATGAAGAGCTAATAAAAGATGGAGAAGAGAATAGCGGAACACAAGTGTTATACAAAAAATACATAGGGGGTAAGT
GA

Upstream 100 bases:

>100_bases
ATGTAATGAGAAGTTTTGGTTGGAGATAAAGAACGTGTTATATTGAATAAAGAGTTCTGAAATATAGTAAGAGAGTTATT
AGAACAAAGGGGATGAGAAA

Downstream 100 bases:

>100_bases
GATGGATCTTCAAAAATTTGATGAGATGATTGATGCTGTACAACGAGCAACTTGTGTACAAATTAATGAAAAACAAATAG
AAGCTTTTAAACAAAAATAT

Product: 2-hydroxy-3-oxopropionate reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVYF
GIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYTLDAPVSGGDVGAKEARLAIMVGGEKEIYEKCLPLFEKLGT
NIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKAGLDPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
FMKDMKIALDEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK

Sequences:

>Translated_293_residues
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVYF
GIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYTLDAPVSGGDVGAKEARLAIMVGGEKEIYEKCLPLFEKLGT
NIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKAGLDPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
FMKDMKIALDEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK
>Mature_293_residues
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVYF
GIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYTLDAPVSGGDVGAKEARLAIMVGGEKEIYEKCLPLFEKLGT
NIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKAGLDPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
FMKDMKIALDEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=27.208480565371, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI23308751, Length=276, Percent_Identity=29.3478260869565, Blast_Score=138, Evalue=7e-33,
Organism=Escherichia coli, GI145693186, Length=286, Percent_Identity=35.6643356643357, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1786719, Length=275, Percent_Identity=37.4545454545455, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1790315, Length=267, Percent_Identity=28.8389513108614, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789092, Length=264, Percent_Identity=25.3787878787879, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=28.3636363636364, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321695, Length=245, Percent_Identity=26.9387755102041, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24655230, Length=289, Percent_Identity=33.9100346020761, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI19922568, Length=289, Percent_Identity=33.9100346020761, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI28574115, Length=286, Percent_Identity=27.6223776223776, Blast_Score=131, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31852; Mature: 31852

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVK
CCCCEEEEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHH
QVDVVMTMVGYPHDVEEVYFGIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYT
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEE
LDAPVSGGDVGAKEARLAIMVGGEKEIYEKCLPLFEKLGTNIQLQGPAGSGQHTKMCNQI
EECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
AIASNMIGVCEAVAYAKKAGLDPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHCHHHHCCCCCCCHHHHH
FMKDMKIALDEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK
HHHHHHHHHCCHHHEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVK
CCCCEEEEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHH
QVDVVMTMVGYPHDVEEVYFGIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYT
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEE
LDAPVSGGDVGAKEARLAIMVGGEKEIYEKCLPLFEKLGTNIQLQGPAGSGQHTKMCNQI
EECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
AIASNMIGVCEAVAYAKKAGLDPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHCHHHHCCCCCCCHHHHH
FMKDMKIALDEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK
HHHHHHHHHCCHHHEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]