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The map label for this gene is bmr3 [H]

Identifier: 218905134

GI number: 218905134

Start: 3847254

End: 3848795

Strand: Direct

Name: bmr3 [H]

Synonym: BCAH820_4018

Alternate gene names: 218905134

Gene position: 3847254-3848795 (Clockwise)

Preceding gene: 218905132

Following gene: 218905135

Centisome position: 72.55

GC content: 38.39

Gene sequence:

>1542_bases
TTGGAATCTCAATTATTAAAAAAAGACTCCAACAAGACAAAATTTGTTGTTGCTGGTTTACTACTTGGTATTTTAATGGC
GGCAATGGATAATACAATTGTTGCAACAGCAATGGCAACAATCGTTGGAGATCTAGGAGGATTTGACAAGTTCGTTTGGG
TCACGTCTGCATATATGGTAGCAACAATGGCAGGGATGCCTATCTTCGGAAAACTTTCTGATATGTATGGACGGAAACGC
TTTTATATTGGTGGCCTTATTCTTTTCTTGTTCGGCTCTGCACTTTGCGGAACAGCATCTAGTATTGAGCAACTAAGTAT
TTATAGAGCAATTCAAGGAATTGGTGGCGGCGCTCTTATGCCTATCGCCTTCACAATTATGTACGATATATTCCCACCAG
AAAAGCGTGGAAAAATGACAGGGCTATTCGGTGCTGTTTTCGGGACATCTAGTGTATTCGGTCCTTTATTAGGTGCGTAT
ATTACAGACTATATAAGTTGGCACTGGGTCTTCTATATTAATATCCCATTAGGACTTATCTCCTTCTTCTTCATTACGAA
ATACTACAGTGAATCTCTAGAATTTAGAAAACAAAAAATTGATTGGGCTGGCGCTATTACACTTGTTATTAGTATCGTTT
GCTTAATGTTCGCCTTAGAACTTGGTGGTAAAGAATACGCATGGAATTCCACTGTAATTATCGGCTTATTTACAACCGTT
GTTATTATGCTTATTATTTTCTTCTTCGTAGAACGAAAAGCAACCGAGCCTATCATTTCTTTCCATCTATTTAAAAAACC
TTTATTCGCAGCTAGCCAAGGTGTTGCCTTTTTCTATGGTGCCGCTTTTATCATCTGTACAGTTTATATCCCAATCTTCG
TTCAAGGTGTTCTCGGTGGTTCAGCCTCAAATGCTGGATTGATATTAACACCTATGATGGTTGGCTCTGTAATTGGAAGT
CAAACAGGTGGACAGTTAGCTTCTCGAACAAGTTATCGTAACATTATGATGGTATCTGGAGTTTTCTTCGTTCTTGGTAT
TTATTTACTAAGCACTTTAACAATGGACACACCTCGCCTACTCGTAACACTATTTATGGTTCTCGCTGGGCTTGGAGTTG
GCTTCTCATTCTCAGTTTTAAGTATGTCTTCTATCCACAAATTAGAAATGAGAGACCGTGGTTCTGCTACATCAACAAAC
TCATTCTTCCGTTCACTCGGTATGACTCTTGGTGTAACGATTTTTGGTACAATTCAAAATCATATTTTTACAGATAAACT
AAATACTTTGTTCCCTCCTGAACTAGCTAAATTAGCTCCAAAGGGCGGAGACACAAGTTTCTTATTATCACCAGGTGCTA
CCGATAAAATACCAGCTGAAATATTAACTGGTATTAAAGAAGCTCTCGCTACATCTATTGCCAGTACATTCTTCTGGGCA
CTTATCCCGGCTGTACTAAGCATTATTTGCATTTTACTTATGGGAAATGAGCGTCTTTTTACAGGACCAAAAACGAAACA
AAAACAGAAGCAGGTAAGTTAA

Upstream 100 bases:

>100_bases
AGTGCACTTGTATAAATTGATAAAAAAACTATACTTTTAATTTTTATGCGCTGCACTCAAAACTTAATATAGTTTACTTA
TTATTAAAGGAGGGTTATCT

Downstream 100 bases:

>100_bases
TATATAGAAAAGAAACGGCATATCCCTTTATGAAAACGGATATGCCGTTTATAATATGTATAAAGTTTTTATATATAGGA
GTTGAAACAGCATGAGTATG

Product: drug resistance transporter, EmrB/QacA family

Products: NA

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MESQLLKKDSNKTKFVVAGLLLGILMAAMDNTIVATAMATIVGDLGGFDKFVWVTSAYMVATMAGMPIFGKLSDMYGRKR
FYIGGLILFLFGSALCGTASSIEQLSIYRAIQGIGGGALMPIAFTIMYDIFPPEKRGKMTGLFGAVFGTSSVFGPLLGAY
ITDYISWHWVFYINIPLGLISFFFITKYYSESLEFRKQKIDWAGAITLVISIVCLMFALELGGKEYAWNSTVIIGLFTTV
VIMLIIFFFVERKATEPIISFHLFKKPLFAASQGVAFFYGAAFIICTVYIPIFVQGVLGGSASNAGLILTPMMVGSVIGS
QTGGQLASRTSYRNIMMVSGVFFVLGIYLLSTLTMDTPRLLVTLFMVLAGLGVGFSFSVLSMSSIHKLEMRDRGSATSTN
SFFRSLGMTLGVTIFGTIQNHIFTDKLNTLFPPELAKLAPKGGDTSFLLSPGATDKIPAEILTGIKEALATSIASTFFWA
LIPAVLSIICILLMGNERLFTGPKTKQKQKQVS

Sequences:

>Translated_513_residues
MESQLLKKDSNKTKFVVAGLLLGILMAAMDNTIVATAMATIVGDLGGFDKFVWVTSAYMVATMAGMPIFGKLSDMYGRKR
FYIGGLILFLFGSALCGTASSIEQLSIYRAIQGIGGGALMPIAFTIMYDIFPPEKRGKMTGLFGAVFGTSSVFGPLLGAY
ITDYISWHWVFYINIPLGLISFFFITKYYSESLEFRKQKIDWAGAITLVISIVCLMFALELGGKEYAWNSTVIIGLFTTV
VIMLIIFFFVERKATEPIISFHLFKKPLFAASQGVAFFYGAAFIICTVYIPIFVQGVLGGSASNAGLILTPMMVGSVIGS
QTGGQLASRTSYRNIMMVSGVFFVLGIYLLSTLTMDTPRLLVTLFMVLAGLGVGFSFSVLSMSSIHKLEMRDRGSATSTN
SFFRSLGMTLGVTIFGTIQNHIFTDKLNTLFPPELAKLAPKGGDTSFLLSPGATDKIPAEILTGIKEALATSIASTFFWA
LIPAVLSIICILLMGNERLFTGPKTKQKQKQVS
>Mature_513_residues
MESQLLKKDSNKTKFVVAGLLLGILMAAMDNTIVATAMATIVGDLGGFDKFVWVTSAYMVATMAGMPIFGKLSDMYGRKR
FYIGGLILFLFGSALCGTASSIEQLSIYRAIQGIGGGALMPIAFTIMYDIFPPEKRGKMTGLFGAVFGTSSVFGPLLGAY
ITDYISWHWVFYINIPLGLISFFFITKYYSESLEFRKQKIDWAGAITLVISIVCLMFALELGGKEYAWNSTVIIGLFTTV
VIMLIIFFFVERKATEPIISFHLFKKPLFAASQGVAFFYGAAFIICTVYIPIFVQGVLGGSASNAGLILTPMMVGSVIGS
QTGGQLASRTSYRNIMMVSGVFFVLGIYLLSTLTMDTPRLLVTLFMVLAGLGVGFSFSVLSMSSIHKLEMRDRGSATSTN
SFFRSLGMTLGVTIFGTIQNHIFTDKLNTLFPPELAKLAPKGGDTSFLLSPGATDKIPAEILTGIKEALATSIASTFFWA
LIPAVLSIICILLMGNERLFTGPKTKQKQKQVS

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=412, Percent_Identity=27.9126213592233, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789042, Length=414, Percent_Identity=28.743961352657, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788710, Length=413, Percent_Identity=28.8135593220339, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790195, Length=425, Percent_Identity=27.2941176470588, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788392, Length=431, Percent_Identity=27.1461716937355, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788509, Length=117, Percent_Identity=28.2051282051282, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790146, Length=133, Percent_Identity=29.3233082706767, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=482, Percent_Identity=24.896265560166, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319770, Length=390, Percent_Identity=30, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6323735, Length=523, Percent_Identity=24.0917782026769, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319783, Length=396, Percent_Identity=24.4949494949495, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321663, Length=458, Percent_Identity=23.5807860262009, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325455, Length=455, Percent_Identity=22.6373626373626, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324954, Length=394, Percent_Identity=23.6040609137056, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55701; Mature: 55701

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQLLKKDSNKTKFVVAGLLLGILMAAMDNTIVATAMATIVGDLGGFDKFVWVTSAYMV
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ATMAGMPIFGKLSDMYGRKRFYIGGLILFLFGSALCGTASSIEQLSIYRAIQGIGGGALM
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHH
PIAFTIMYDIFPPEKRGKMTGLFGAVFGTSSVFGPLLGAYITDYISWHWVFYINIPLGLI
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEEECHHHHH
SFFFITKYYSESLEFRKQKIDWAGAITLVISIVCLMFALELGGKEYAWNSTVIIGLFTTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHH
VIMLIIFFFVERKATEPIISFHLFKKPLFAASQGVAFFYGAAFIICTVYIPIFVQGVLGG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SASNAGLILTPMMVGSVIGSQTGGQLASRTSYRNIMMVSGVFFVLGIYLLSTLTMDTPRL
CCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LVTLFMVLAGLGVGFSFSVLSMSSIHKLEMRDRGSATSTNSFFRSLGMTLGVTIFGTIQN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEHHHH
HIFTDKLNTLFPPELAKLAPKGGDTSFLLSPGATDKIPAEILTGIKEALATSIASTFFWA
HHHHHHHCCCCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIPAVLSIICILLMGNERLFTGPKTKQKQKQVS
HHHHHHHHHHHHHCCCCEEECCCCCHHHHHCCC
>Mature Secondary Structure
MESQLLKKDSNKTKFVVAGLLLGILMAAMDNTIVATAMATIVGDLGGFDKFVWVTSAYMV
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ATMAGMPIFGKLSDMYGRKRFYIGGLILFLFGSALCGTASSIEQLSIYRAIQGIGGGALM
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHH
PIAFTIMYDIFPPEKRGKMTGLFGAVFGTSSVFGPLLGAYITDYISWHWVFYINIPLGLI
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEEECHHHHH
SFFFITKYYSESLEFRKQKIDWAGAITLVISIVCLMFALELGGKEYAWNSTVIIGLFTTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHH
VIMLIIFFFVERKATEPIISFHLFKKPLFAASQGVAFFYGAAFIICTVYIPIFVQGVLGG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SASNAGLILTPMMVGSVIGSQTGGQLASRTSYRNIMMVSGVFFVLGIYLLSTLTMDTPRL
CCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LVTLFMVLAGLGVGFSFSVLSMSSIHKLEMRDRGSATSTNSFFRSLGMTLGVTIFGTIQN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEHHHH
HIFTDKLNTLFPPELAKLAPKGGDTSFLLSPGATDKIPAEILTGIKEALATSIASTFFWA
HHHHHHHCCCCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIPAVLSIICILLMGNERLFTGPKTKQKQKQVS
HHHHHHHHHHHHHCCCCEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]