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The map label for this gene is def

Identifier: 218905105

GI number: 218905105

Start: 3823568

End: 3824122

Strand: Direct

Name: def

Synonym: BCAH820_3989

Alternate gene names: 218905105

Gene position: 3823568-3824122 (Clockwise)

Preceding gene: 218905104

Following gene: 218905107

Centisome position: 72.11

GC content: 36.94

Gene sequence:

>555_bases
ATGCTTACAATGAAAGATGTAATTCGCGAAGGAGATCCTATTTTGCGAAACGTTGCAGAAGAGGTAGTAATACCAGCGAG
CGAAGAAGATACAAATACCCTTAAAGAAATGATTGAATTTGTAATAAATAGCCAAGATCCTGAAATGGCTGAAAAATATA
GTTTACGCCCTGGAATCGGATTAGCGGCTCCGCAAATCGGTATTTCAAAGAAAATGATTGCAGTTCACGTAACAGATACG
GACGGTACGTTATATAGTCATGCATTATTCAATCCAAAAATCATTAGCCATTCTGTTGAACGTACATATTTACAAAGTGG
TGAAGGCTGTCTATCAGTAGACCGTGAAGTACCTGGTTATGTACCTCGTTATACAAGAATTACAGTGAAAGCAACTTCTA
TCAACGGCGAAGAAGTAAAATTACGTTTAAAAGGTTTACCAGCAATTGTATTCCAACATGAAATTGACCATTTAAATGGT
GTTATGTTCTATGACCATATTAATAAAGAAAATCCATTTGCTGCTCCTGACGGTTCAAAACCTCTGGAGCGATAA

Upstream 100 bases:

>100_bases
GTTTCTTTTTATTCCTACATAACCGACCTATCTTTCCCTTACTTGTCGATGACTTTTTCCATCTCCTTTGGTAGACTAAA
GGAGAAAGGAGAGATTACAT

Downstream 100 bases:

>100_bases
TAAAAAAAGGCGGAAGATGATTCTCCGCCTTTTTACTATTATGAAATGGGTTCCATATCCACTTATAACAATCCAGCCCA
TTTTAATCCATGATATATGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGISKKMIAVHVTDT
DGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNG
VMFYDHINKENPFAAPDGSKPLER

Sequences:

>Translated_184_residues
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGISKKMIAVHVTDT
DGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNG
VMFYDHINKENPFAAPDGSKPLER
>Mature_184_residues
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGISKKMIAVHVTDT
DGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNG
VMFYDHINKENPFAAPDGSKPLER

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=122, Percent_Identity=36.8852459016393, Blast_Score=71, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24645728, Length=174, Percent_Identity=28.1609195402299, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_BACAN (Q81MQ9)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_846423.1
- RefSeq:   YP_020831.1
- RefSeq:   YP_030134.1
- ProteinModelPortal:   Q81MQ9
- SMR:   Q81MQ9
- EnsemblBacteria:   EBBACT00000010470
- EnsemblBacteria:   EBBACT00000016119
- EnsemblBacteria:   EBBACT00000020183
- GeneID:   1088919
- GeneID:   2818161
- GeneID:   2850259
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4187
- KEGG:   bar:GBAA_4187
- KEGG:   bat:BAS3884
- TIGR:   BA_4187
- TIGR:   GBAA_4187
- GeneTree:   EBGT00050000001175
- HOGENOM:   HBG665227
- OMA:   DIIKEGH
- ProtClustDB:   PRK00150
- BioCyc:   BANT260799:BAS3884-MONOMER
- BioCyc:   BANT261594:GBAA4187-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20516; Mature: 20516

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIG
CCCHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
LAAPQIGISKKMIAVHVTDTDGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGY
CCCCCCCCCCEEEEEEEECCCCCEEEHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCC
VPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNGVMFYDHINKENPFAAPDGSK
CCCEEEEEEEEEECCCCEEEEEECCCCEEEEEHHHHHCCCEEEEEECCCCCCCCCCCCCC
PLER
CCCC
>Mature Secondary Structure
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIG
CCCHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
LAAPQIGISKKMIAVHVTDTDGTLYSHALFNPKIISHSVERTYLQSGEGCLSVDREVPGY
CCCCCCCCCCEEEEEEEECCCCCEEEHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCC
VPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNGVMFYDHINKENPFAAPDGSK
CCCEEEEEEEEEECCCCEEEEEECCCCEEEEEHHHHHCCCEEEEEECCCCCCCCCCCCCC
PLER
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629