Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 218905049

GI number: 218905049

Start: 3773991

End: 3774923

Strand: Reverse

Name: mraW

Synonym: BCAH820_3933

Alternate gene names: 218905049

Gene position: 3774923-3773991 (Counterclockwise)

Preceding gene: 218905050

Following gene: 218905048

Centisome position: 71.19

GC content: 37.83

Gene sequence:

>933_bases
ATGTTTAATCACGTAACAGTGCTTTTGAAGGAAACAGTAGATGGCTTAGATATAAAGCCAGATGGTACATATGTAGATTG
TACACTGGGGGGAGGAGGACATAGTTCTTATTTATTATCCCAATTAACTGAAGGCGGAAGATTAATCGCGTTTGATCAAG
ATGAGATAGCAATTCAAAATGCGAAAGAAAAATTCTCTTCATACGGTGAACAGTTCATAACAGTAAAGAGTAATTTCCGT
TACCTATCTGAAAAACTACAGGAATTAGGTATAACGGAAGTAGACGGTATTTTATTTGATTTAGGTGTTTCATCCCCGCA
ATTAGATACGCCAGAGCGTGGCTTTAGTTACCATCATGATGCACCGTTAGATATGCGAATGGATCAAGATGCCCCATTAA
CAGCATATGATGTTGTAAATAGTTGGTCATATGAACAACTTGTAAGAATTTTCTTCCAGTATGGCGAAGAGAAATTTTCA
AAACAAATAGCAAGGAAAATTGAAGCGTATCGTGAGAATAAAGCTATTGAAACGACAGGGGAATTAGTAGAACTAATTAA
AGAGGGTATTCCAGCTCCTGCTCGTAGAACGGGTGGACATCCTGCGAAGAGAGTGTTCCAAGCAATCCGTATTGCAGTAA
ATGATGAATTGAAAGTATTTGAAGAAGCGTTGGAATCTGCAATTGAGATGGTCAAGCCAGGCGGAAGAGTTAGTGTTATA
ACATTCCATTCTTTAGAAGACCGTATTTGCAAAACGACGTTTAAACGAAATAGTACAACGCCGCAATTGCCGCCAGGATT
ACCAATTATTCCTGATGAATTTAAGCCGAAGTTAAAGCTTATTACAAGAAAACCGATTTTACCTTCTGATATAGAGTTAG
AAGAAAATAATCGAGCGCGTTCTGCGAAATTGAGAATCGCTGAAAAACGATAA

Upstream 100 bases:

>100_bases
AGTCGTTCTTTCATAGTATTTCGTTCTCTAGTCGTGAAATATGCGTTTAGAAGAGGAGATACATTCAACATAATTAATTG
AAAAAAGAGTAGGTGCAACA

Downstream 100 bases:

>100_bases
AAAGGGGAGAGAGGTATGAGTAACTTAGCTGTAAAGTACAAACAACAAGCGCAAGAAGAGGTTCAGATTCAAACGCCCCC
ACAGCAAATGGTTCAGCCAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQNAKEKFSSYGEQFITVKSNFR
YLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFS
KQIARKIEAYRENKAIETTGELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRARSAKLRIAEKR

Sequences:

>Translated_310_residues
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQNAKEKFSSYGEQFITVKSNFR
YLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFS
KQIARKIEAYRENKAIETTGELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRARSAKLRIAEKR
>Mature_310_residues
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQNAKEKFSSYGEQFITVKSNFR
YLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFS
KQIARKIEAYRENKAIETTGELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRARSAKLRIAEKR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=35.3982300884956, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI165377202, Length=191, Percent_Identity=38.2198952879581, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=45.5128205128205, Blast_Score=253, Evalue=8e-69,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=37.5757575757576, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=37.5757575757576, Blast_Score=171, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_BACAA (C3P688)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868154.1
- ProteinModelPortal:   C3P688
- EnsemblBacteria:   EBBACT00000127205
- GeneID:   7850154
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4083
- GeneTree:   EBGT00050000002010
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34959; Mature: 34959

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 79-79 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQN
CCCHHEEHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHHH
AKEKFSSYGEQFITVKSNFRYLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHD
HHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFSKQIARKIEAYRENKAIETTG
CCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRAR
EECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCC
SAKLRIAEKR
HHEEEEECCC
>Mature Secondary Structure
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQN
CCCHHEEHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHHH
AKEKFSSYGEQFITVKSNFRYLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHD
HHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFSKQIARKIEAYRENKAIETTG
CCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRAR
EECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCC
SAKLRIAEKR
HHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA