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The map label for this gene is sigB [H]

Identifier: 218902186

GI number: 218902186

Start: 1027222

End: 1027995

Strand: Direct

Name: sigB [H]

Synonym: BCAH820_1068

Alternate gene names: 218902186

Gene position: 1027222-1027995 (Clockwise)

Preceding gene: 218902185

Following gene: 218902187

Centisome position: 19.37

GC content: 35.79

Gene sequence:

>774_bases
ATGGAAATCCAATCTCAACCTACGAATCTTACTAAAGAAGACGTTATTAAACTAATTGCAGAATTTCAACAAAACCAATG
TGGTGAAGCGCAGGAAAGGTTAGTTGATCATTATAAAAATCTCGTATATTCCATTGCATATCGCTATTCAAAAGGCGGGC
CAATGCATGAGGATATTATACAAGTAGGCATGTTAGGGCTCTTAGGCGCAATAAGAAGGTATGATTATTCGATAGGGAAT
GCTTTTGAGCCTTTTGCAATACCTACAATAGTAGGGGAAATAAAGAAGTATTTACGCGATAAAACTTGGGGCATTCACGT
TCCAAGACGAATTAAAGATTTAGGCGGAAAAATTAAACTTGCGATAGAGGAACTAACAGATCATTTGCAACGTTCACCGA
AGATTATAGAGATTGCGGATCATTTAGGGCTATCCGAAGAGGAAGTGCTAGAAATTATGGATGCGAAAAATAATTATCGA
GTATCTTCCTTAGATGATGTAGTTGAAAATGCATCTGATGGTAGTTCGGTAGCGAGAATTGAATCTGTAGGTGAAGTAGA
GCAAGGATATGAAGAGACAGAAAGGCGTCTCGTTTTAAAGGATATTTTTAACGTATTAAATGAAACGGAAAAGAGTGTTA
TTCATTATATATTTGAAGAAAATTTAAATCAAAAAGATACAGGGGAACGGTTAGGTATTTCACAAATGCACGTTTCTCGT
ATTAAAAGACAAGCGATAAGTAAGCTGAAACAAGCCGCATTTTTAGATACATAA

Upstream 100 bases:

>100_bases
TATTTAATCAATACGTTAATGGATGATATACAAATCATGCATGATGAGGGCATGACGGTTTTAATGACAAAATATATACA
AAGAGAGCAGGTGGAGAATG

Downstream 100 bases:

>100_bases
AAAATTTAAAATAATGATGTTTAAAACATGAGAAAAGGGGTACTTATTAAGTATAGGAGGAGATATGAAATGTCACACGA
TGTGAAAGAACTAATCGAAG

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MEIQSQPTNLTKEDVIKLIAEFQQNQCGEAQERLVDHYKNLVYSIAYRYSKGGPMHEDIIQVGMLGLLGAIRRYDYSIGN
AFEPFAIPTIVGEIKKYLRDKTWGIHVPRRIKDLGGKIKLAIEELTDHLQRSPKIIEIADHLGLSEEEVLEIMDAKNNYR
VSSLDDVVENASDGSSVARIESVGEVEQGYEETERRLVLKDIFNVLNETEKSVIHYIFEENLNQKDTGERLGISQMHVSR
IKRQAISKLKQAAFLDT

Sequences:

>Translated_257_residues
MEIQSQPTNLTKEDVIKLIAEFQQNQCGEAQERLVDHYKNLVYSIAYRYSKGGPMHEDIIQVGMLGLLGAIRRYDYSIGN
AFEPFAIPTIVGEIKKYLRDKTWGIHVPRRIKDLGGKIKLAIEELTDHLQRSPKIIEIADHLGLSEEEVLEIMDAKNNYR
VSSLDDVVENASDGSSVARIESVGEVEQGYEETERRLVLKDIFNVLNETEKSVIHYIFEENLNQKDTGERLGISQMHVSR
IKRQAISKLKQAAFLDT
>Mature_257_residues
MEIQSQPTNLTKEDVIKLIAEFQQNQCGEAQERLVDHYKNLVYSIAYRYSKGGPMHEDIIQVGMLGLLGAIRRYDYSIGN
AFEPFAIPTIVGEIKKYLRDKTWGIHVPRRIKDLGGKIKLAIEELTDHLQRSPKIIEIADHLGLSEEEVLEIMDAKNNYR
VSSLDDVVENASDGSSVARIESVGEVEQGYEETERRLVLKDIFNVLNETEKSVIHYIFEENLNQKDTGERLGISQMHVSR
IKRQAISKLKQAAFLDT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=196, Percent_Identity=30.1020408163265, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789098, Length=241, Percent_Identity=24.0663900414938, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29303; Mature: 29303

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]