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The map label for this gene is alsS [H]

Identifier: 218902076

GI number: 218902076

Start: 914050

End: 915738

Strand: Direct

Name: alsS [H]

Synonym: BCAH820_0958

Alternate gene names: 218902076

Gene position: 914050-915738 (Clockwise)

Preceding gene: 218902073

Following gene: 218902077

Centisome position: 17.24

GC content: 40.85

Gene sequence:

>1689_bases
TTGAGTACAGGTGTAAAAGCAAACGACGTGAAGACAAAAACAAAAGGAGCAGATCTTGTTGTTGATTGTTTAATTAAACA
AGGTGTTACACATGTTTTCGGTATTCCAGGAGCGAAGATTGACTCTGTATTTGATGTACTGCAAGAAAGAGGACCAGAGT
TAATTGTTTGTCGTCATGAACAAAATGCAGCATTTATGGCAGCTGCGATTGGTAGATTAACAGGAAAACCGGGCGTATGT
CTTGTAACTTCAGGGCCAGGGACATCAAATTTAGCGACAGGTCTTGTTACTGCGAATGCGGAGAGTGATCCCGTTGTTGC
TTTAGCTGGTGCAGTTCCGCGTACAGATCGATTAAAACGTACGCACCAATCTATGGATAATGCTGCACTATTCGAACCAA
TCACAAAATATAGCGTAGAAGTAGAGCATCCTGATAATGTGCCAGAAGCATTATCAAATGCATTCAGAAGTGCAACTTCT
ACAAATCCAGGCGCTACTTTAGTAAGTTTGCCGCAAGACGTTATGACTGCGGAAACAACTGTAGAGTCTATCGGTGCACT
TTCTAAGCCACAGCTTGGAATCGCTCCCACACATGATATTACATACGTAGTAGAAAAAATAAAAGCAGCGAAATTACCAG
TTATTTTACTCGGTATGAGAGCGAGTACGAATGAAGTGACGAAAGCTGTTCGTGCATTAATTGCGGATACAGAGCTTCCT
GTCGTTGAAACATATCAAGCAGCTGGTGCGATTTCACGTGAGTTAGAAGATCATTTCTTCGGCCGCGTTGGATTATTCCG
TAACCAACCAGGTGATATTTTACTAGAAGAAGCGGACCTTGTTATTTCTATCGGTTATGACCCAATTGAGTATGATCCGA
AATTCTGGAATAAACTAGGGGACAGAACGATTATTCATCTTGATGACCATCAAGCAGATATCGATCATGATTACCAACCA
GAGCGTGAATTAATTGGTGATATTGCCTTAACGGTAAATAGCATCGCAGAAAGATTACCGAAACTTGTGTTAAGTACGAA
ATCAGAAGCAGTGTTAGAACGATTACGCGCGAAATTATCAGAACAAGCAGAAGTTCCAAACCGTGATTCAGAAGGTGTTA
CGCATCCACTTCAAGTGATTCGTACACTTCGTTCTTTAATTAGTGACGATACAACCGTTACATGTGACATCGGTTCCCAT
TCTATTTGGATGGCGAGATGTTTCCGTTCTTATGAACCACGTAGATTATTATTTAGTAACGGTATGCAAACGTTAGGTGT
TGCACTTCCTTGGGCAATCGCAGCTACTTTAGTAGAACCAGGTAAAAAAGTAGTTTCTGTATCAGGTGACGGTGGTTTCT
TATTCTCAGCGATGGAGTTAGAAACGGCGGTACGTTTAAATTCTCCAATCGTCCATCTCGTTTGGAGAGACGGCACATAT
GATATGGTTGCATTCCAACAAATGATGAAATACGGCAGAACATCAGCTACAGAGTTTGGTGATGTTGATCTTGTGAAATA
TGCGGAAAGTTTCGGTGCGACAGGTCTTCGCGTTAACACGCCTGATGAATTAGAAGGTGTATTAAAATCCGCATTAGCAG
CAGACGGTCCTGTCATTATTGATATTCCAATTGACTATCGTGACAATATTAAATTAAGCGAAAAATTATTACCAAACCAA
TTAAACTAA

Upstream 100 bases:

>100_bases
ATTTGTACTATAATGATGAATAATACTTCACCACATTAATTAACTAGGTGAAGTTTAAAGTCTTTATTACACACATTATG
AAATAAAGGAATGATTAGTT

Downstream 100 bases:

>100_bases
TGGAGGCAGATTTGAGATGACCGTTGCGCAATTAATTGATATTGATGCAAAAAGAACGAAAACGAATAACGAAGTATATC
AAACATCTACAATGCTTGCG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKPGVC
LVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATS
TNPGATLVSLPQDVMTAETTVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRALIADTELP
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHDYQP
ERELIGDIALTVNSIAERLPKLVLSTKSEAVLERLRAKLSEQAEVPNRDSEGVTHPLQVIRTLRSLISDDTTVTCDIGSH
SIWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
DMVAFQQMMKYGRTSATEFGDVDLVKYAESFGATGLRVNTPDELEGVLKSALAADGPVIIDIPIDYRDNIKLSEKLLPNQ
LN

Sequences:

>Translated_562_residues
MSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKPGVC
LVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATS
TNPGATLVSLPQDVMTAETTVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRALIADTELP
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHDYQP
ERELIGDIALTVNSIAERLPKLVLSTKSEAVLERLRAKLSEQAEVPNRDSEGVTHPLQVIRTLRSLISDDTTVTCDIGSH
SIWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
DMVAFQQMMKYGRTSATEFGDVDLVKYAESFGATGLRVNTPDELEGVLKSALAADGPVIIDIPIDYRDNIKLSEKLLPNQ
LN
>Mature_561_residues
STGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKPGVCL
VTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATST
NPGATLVSLPQDVMTAETTVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRALIADTELPV
VETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHDYQPE
RELIGDIALTVNSIAERLPKLVLSTKSEAVLERLRAKLSEQAEVPNRDSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHS
IWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTYD
MVAFQQMMKYGRTSATEFGDVDLVKYAESFGATGLRVNTPDELEGVLKSALAADGPVIIDIPIDYRDNIKLSEKLLPNQL
N

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=26.7256637168142, Blast_Score=192, Evalue=1e-48,
Organism=Homo sapiens, GI21361361, Length=595, Percent_Identity=25.0420168067227, Blast_Score=150, Evalue=3e-36,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=29.2253521126761, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=29.3260473588342, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1786717, Length=576, Percent_Identity=27.7777777777778, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1787096, Length=537, Percent_Identity=26.4432029795158, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=27.158273381295, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=577, Percent_Identity=26.5164644714038, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=551, Percent_Identity=26.8602540834846, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=554, Percent_Identity=27.0758122743682, Blast_Score=148, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=27.3049645390071, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6320816, Length=539, Percent_Identity=27.6437847866419, Blast_Score=143, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323163, Length=548, Percent_Identity=23.3576642335766, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922626, Length=557, Percent_Identity=26.2118491921005, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60970; Mature: 60839

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHE
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCEEEEECC
QNAAFMAAAIGRLTGKPGVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKR
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHH
THQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATSTNPGATLVSLPQDVMTAETT
HHHHCCCHHHHCCHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
VESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRALIADTELP
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCC
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCHHHHHHCC
DRTIIHLDDHQADIDHDYQPERELIGDIALTVNSIAERLPKLVLSTKSEAVLERLRAKLS
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EQAEVPNRDSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHSIWMARCFRSYEPRRLLFSN
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHC
GMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCH
DMVAFQQMMKYGRTSATEFGDVDLVKYAESFGATGLRVNTPDELEGVLKSALAADGPVII
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEEE
DIPIDYRDNIKLSEKLLPNQLN
EEECCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
STGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHE
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCEEEEECC
QNAAFMAAAIGRLTGKPGVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKR
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHH
THQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATSTNPGATLVSLPQDVMTAETT
HHHHCCCHHHHCCHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
VESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRALIADTELP
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCC
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCHHHHHHCC
DRTIIHLDDHQADIDHDYQPERELIGDIALTVNSIAERLPKLVLSTKSEAVLERLRAKLS
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EQAEVPNRDSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHSIWMARCFRSYEPRRLLFSN
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHC
GMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCH
DMVAFQQMMKYGRTSATEFGDVDLVKYAESFGATGLRVNTPDELEGVLKSALAADGPVII
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEEE
DIPIDYRDNIKLSEKLLPNQLN
EEECCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]