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The map label for this gene is pcnB2 [H]

Identifier: 218884730

GI number: 218884730

Start: 1316942

End: 1317691

Strand: Direct

Name: pcnB2 [H]

Synonym: DKAM_1419

Alternate gene names: 218884730

Gene position: 1316942-1317691 (Clockwise)

Preceding gene: 218884729

Following gene: 218884731

Centisome position: 96.46

GC content: 45.47

Gene sequence:

>750_bases
ATGAGGCTTAAGTTTAGTGACGCAGTTGCATGGAGATATGCTATAACATCCATCTCTAAGATTATTGATGAAGCAAACTT
TAGGATTAGCGGTGAAGACGGATTATTATTGAAAGCTATGGATCCCAGTACTGTTGTACTTGTAGAGTTCGCAATACCGA
AGGAGGCTTTTTCAGAGTACAGTGTGGAAAGGGAACTTGTAGTAGGGGTAAGAATGGATGATCTGACCAAGGTGTTGAAG
AGGGCTAGGAGAGGTGATGAACTCGTTTTAGAAGTGCTAGAAGACGGCAGGCTAAGCGTAGTCTTCGAGGGTAGGGGTGT
CAGAAGATTTACCCTGCCAAGCATAGAGATGACTTACGAGGAGATACCGGAGATTAGTTTCGAAGTAGGGTTTAAGGGGA
AGCTTCTTCCCAAGTTGTTTAGAGACATAGTTAGGGAGGTAGAGCCCATCAGCGATTCCATCGAGTTTTCAGCCAGCAAG
GACAGCGATAAGCTGACTGTCTCATCGAGGAGTGAAATAGCTGAGGCTGAAATAGAGCTCTCAGCCTCAGATGGTGCGTT
AATCGAGTACCAGGTGCTAAGTGATGCAAGGGCTAAATACACGATCGACTATCTTATCGATATATCGGTTGCTTCTCAGG
CTGCTGAATTAATGGAGATAGAATTCGGTAATGGAACACCGCTCAAGCTTACATACGAGATACCCGGCGGAGGAAGACTA
GTGTTTTATGTTGCCCCACGGGAAGACTAG

Upstream 100 bases:

>100_bases
TACAAAGTGCGGTTACACTTGGAGAGAGTATGCTTAGCATCCTCTGTACTTGATAATTTTATTTATTCGGATAACGGTAA
AAGTATAGTAGGTGGTTCGA

Downstream 100 bases:

>100_bases
CGACTTCTTGAAGAAGGTCATAAGGGACTACTATAGGTTCAGACCTCTCGAAGAACCAGGCAACCTACATATGAGAGAGA
TTGCTTTAGAGAGCCTTGAG

Product: DNA polymerase sliding clamp subunit B2 (PCNA-like protein)

Products: NA

Alternate protein names: Proliferating cell nuclear antigen homolog B2; PCNA B2 [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRLKFSDAVAWRYAITSISKIIDEANFRISGEDGLLLKAMDPSTVVLVEFAIPKEAFSEYSVERELVVGVRMDDLTKVLK
RARRGDELVLEVLEDGRLSVVFEGRGVRRFTLPSIEMTYEEIPEISFEVGFKGKLLPKLFRDIVREVEPISDSIEFSASK
DSDKLTVSSRSEIAEAEIELSASDGALIEYQVLSDARAKYTIDYLIDISVASQAAELMEIEFGNGTPLKLTYEIPGGGRL
VFYVAPRED

Sequences:

>Translated_249_residues
MRLKFSDAVAWRYAITSISKIIDEANFRISGEDGLLLKAMDPSTVVLVEFAIPKEAFSEYSVERELVVGVRMDDLTKVLK
RARRGDELVLEVLEDGRLSVVFEGRGVRRFTLPSIEMTYEEIPEISFEVGFKGKLLPKLFRDIVREVEPISDSIEFSASK
DSDKLTVSSRSEIAEAEIELSASDGALIEYQVLSDARAKYTIDYLIDISVASQAAELMEIEFGNGTPLKLTYEIPGGGRL
VFYVAPRED
>Mature_249_residues
MRLKFSDAVAWRYAITSISKIIDEANFRISGEDGLLLKAMDPSTVVLVEFAIPKEAFSEYSVERELVVGVRMDDLTKVLK
RARRGDELVLEVLEDGRLSVVFEGRGVRRFTLPSIEMTYEEIPEISFEVGFKGKLLPKLFRDIVREVEPISDSIEFSASK
DSDKLTVSSRSEIAEAEIELSASDGALIEYQVLSDARAKYTIDYLIDISVASQAAELMEIEFGNGTPLKLTYEIPGGGRL
VFYVAPRED

Specific function: Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication [H]

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PCNA family [H]

Homologues:

Organism=Homo sapiens, GI33239451, Length=248, Percent_Identity=25.8064516129032, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4505641, Length=248, Percent_Identity=25.8064516129032, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319564, Length=256, Percent_Identity=25, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17136790, Length=258, Percent_Identity=22.093023255814, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45552759, Length=258, Percent_Identity=22.093023255814, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24585303, Length=251, Percent_Identity=23.1075697211155, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000730
- InterPro:   IPR022649
- InterPro:   IPR022648 [H]

Pfam domain/function: PF02747 PCNA_C; PF00705 PCNA_N [H]

EC number: NA

Molecular weight: Translated: 27861; Mature: 27861

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKFSDAVAWRYAITSISKIIDEANFRISGEDGLLLKAMDPSTVVLVEFAIPKEAFSEY
CEEECCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCEEEEEEEECCHHHHHHH
SVERELVVGVRMDDLTKVLKRARRGDELVLEVLEDGRLSVVFEGRGVRRFTLPSIEMTYE
CCCEEEEEEECHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCEEEEECCCEEECHH
EIPEISFEVGFKGKLLPKLFRDIVREVEPISDSIEFSASKDSDKLTVSSRSEIAEAEIEL
HCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEECCCHHHEEEEEEE
SASDGALIEYQVLSDARAKYTIDYLIDISVASQAAELMEIEFGNGTPLKLTYEIPGGGRL
ECCCCCEEEEEEECCCCCEEEEEEEEEEHHHHHHHEEEEEECCCCCCEEEEEECCCCCEE
VFYVAPRED
EEEEECCCC
>Mature Secondary Structure
MRLKFSDAVAWRYAITSISKIIDEANFRISGEDGLLLKAMDPSTVVLVEFAIPKEAFSEY
CEEECCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCEEEEEEEECCHHHHHHH
SVERELVVGVRMDDLTKVLKRARRGDELVLEVLEDGRLSVVFEGRGVRRFTLPSIEMTYE
CCCEEEEEEECHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCEEEEECCCEEECHH
EIPEISFEVGFKGKLLPKLFRDIVREVEPISDSIEFSASKDSDKLTVSSRSEIAEAEIEL
HCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEECCCHHHEEEEEEE
SASDGALIEYQVLSDARAKYTIDYLIDISVASQAAELMEIEFGNGTPLKLTYEIPGGGRL
ECCCCCEEEEEEECCCCCEEEEEEEEEEHHHHHHHEEEEEECCCCCCEEEEEECCCCCEE
VFYVAPRED
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]