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The map label for this gene is atpB [H]

Identifier: 218884309

GI number: 218884309

Start: 944392

End: 945795

Strand: Direct

Name: atpB [H]

Synonym: DKAM_0998

Alternate gene names: 218884309

Gene position: 944392-945795 (Clockwise)

Preceding gene: 218884308

Following gene: 218884310

Centisome position: 69.17

GC content: 44.16

Gene sequence:

>1404_bases
ATGTCGGTGAAATTAACCACAAGGATCTCAAGTAAACTACTTAAAGCACAGGGAGGACTATTATTCGCTGAGACAATACC
TGGTGTAACATATGGTGAAATAGTAGAGGTAACGCTTCCAACAAGCGAGGTGAAACTAGGACAGGTAATAGATGTCAGTA
AAGGGGTGACAGTAATACAGGTATTTGGTGGAGTAGCCGACGTTGATCTATCTAGGTCAAGAGTAAAGTACCGCGGGGAG
ACGCTTAAAATACCTGTAAGCATTGACATGCTTGGAAGAATATTTGATGGATTAGGCAGACCAATAGATGGGGGTCCGAG
GATCATCCCGGAGGATTATCTTGACATAAATGGGGCGCCATTGAACCCAGCTTCGAGGTTACCGCCCTCAGAGTTCATTG
AAACCGGTATTTCAGCTATTGATGGATTATTCAGTATTGTAAGAGGGCAGAAACTCCCAATATTCAGCGGCTCAGGCCTA
CCCCATAATAGGATCGCCGCCCAGATAGTTAGACAGGCAAGAGTTCTCGGTAAAGAGGAGAAGTTCGCCATAGTGTTTGC
AGCTATAGGAGTACCATATGATGATGCAATGTTCTTCATAGATAACTTCAAGAAGACCGGTGCCCTTGAGAGAACTGTTG
CATTAATCAATACAGCTAGTTCACCAGTCATAGAGAGAATTGCAACACCCCGTGTAGCATTAACCATAGCTGAGCACCTA
GCGTGGACGCATGACATGCATGTACTTGTAATATTAACAGACATGACCTTCTACTGTGAAGCCCTTAGAGAACTCAGTGC
CGCAAGAGAAGAAGTCCCAGGTAGAAGAGGATACCCTGGCTACATGTATACTGATTTGGCAACAATATACGAGCGTGCCG
GGCGCGTCCACAGTAAGAAGGGTAGTATAACACAGCTACCAATACTCACCATGCCAGATGATGATATTACACACCCAATA
CCGGATCTCACAGGGTATATAACAGAGGGTCAGCTAGTTCTCTCGAGACAGCTATGGTTGAAGGGTATATATCCACCATT
CGATCCACTAATGAGTCTTTCAAGGTTGATGAAGGATGGAATTGGTGAAGGAAAGACAAGAGAGGACCACAATAATGTAT
TTATGCAGTTAATAAGCGCCTATGCTGAAGGTATAAGACTGAGGGAGCTCTCCGTAATAGTTGGTACGGAAACACTTACT
CAGAGGGATAGGAAGTACCTCGAGTTTGCTGACAGGTTTGAAAGAGAGTTCATTGGACAGGGAGAATATGAGCGGAGGAA
GATAGAGGAGACGCTGGATAAGGGATGGGAGCTTCTAGCTATTCTACCCGAAGAAGAATTAAAGATGATTAAAGTAGAAT
ACATAAGGAAATATCATCCCAAATATAAAGGTGGCAACCCATAG

Upstream 100 bases:

>100_bases
GAATGAAGCATATACCTTTTGGAGATAACACCAAGGAATTTGATAGTATACTTATAGAAATGGAGGAAGAATTCAAGAAT
CTTAGGGAGGAGAGTTAGAT

Downstream 100 bases:

>100_bases
TTGGATACGTGTATACAATCCTTCCACCTAATAGAAATAATTTATATGAACACCTTTTCTCAATAATCTCATGTTTAACG
GTGGCAATTAATGAAGGCCA

Product: V-type ATP synthase subunit B

Products: ADP; phosphate; H+

Alternate protein names: V-ATPase subunit B [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSVKLTTRISSKLLKAQGGLLFAETIPGVTYGEIVEVTLPTSEVKLGQVIDVSKGVTVIQVFGGVADVDLSRSRVKYRGE
TLKIPVSIDMLGRIFDGLGRPIDGGPRIIPEDYLDINGAPLNPASRLPPSEFIETGISAIDGLFSIVRGQKLPIFSGSGL
PHNRIAAQIVRQARVLGKEEKFAIVFAAIGVPYDDAMFFIDNFKKTGALERTVALINTASSPVIERIATPRVALTIAEHL
AWTHDMHVLVILTDMTFYCEALRELSAAREEVPGRRGYPGYMYTDLATIYERAGRVHSKKGSITQLPILTMPDDDITHPI
PDLTGYITEGQLVLSRQLWLKGIYPPFDPLMSLSRLMKDGIGEGKTREDHNNVFMQLISAYAEGIRLRELSVIVGTETLT
QRDRKYLEFADRFEREFIGQGEYERRKIEETLDKGWELLAILPEEELKMIKVEYIRKYHPKYKGGNP

Sequences:

>Translated_467_residues
MSVKLTTRISSKLLKAQGGLLFAETIPGVTYGEIVEVTLPTSEVKLGQVIDVSKGVTVIQVFGGVADVDLSRSRVKYRGE
TLKIPVSIDMLGRIFDGLGRPIDGGPRIIPEDYLDINGAPLNPASRLPPSEFIETGISAIDGLFSIVRGQKLPIFSGSGL
PHNRIAAQIVRQARVLGKEEKFAIVFAAIGVPYDDAMFFIDNFKKTGALERTVALINTASSPVIERIATPRVALTIAEHL
AWTHDMHVLVILTDMTFYCEALRELSAAREEVPGRRGYPGYMYTDLATIYERAGRVHSKKGSITQLPILTMPDDDITHPI
PDLTGYITEGQLVLSRQLWLKGIYPPFDPLMSLSRLMKDGIGEGKTREDHNNVFMQLISAYAEGIRLRELSVIVGTETLT
QRDRKYLEFADRFEREFIGQGEYERRKIEETLDKGWELLAILPEEELKMIKVEYIRKYHPKYKGGNP
>Mature_466_residues
SVKLTTRISSKLLKAQGGLLFAETIPGVTYGEIVEVTLPTSEVKLGQVIDVSKGVTVIQVFGGVADVDLSRSRVKYRGET
LKIPVSIDMLGRIFDGLGRPIDGGPRIIPEDYLDINGAPLNPASRLPPSEFIETGISAIDGLFSIVRGQKLPIFSGSGLP
HNRIAAQIVRQARVLGKEEKFAIVFAAIGVPYDDAMFFIDNFKKTGALERTVALINTASSPVIERIATPRVALTIAEHLA
WTHDMHVLVILTDMTFYCEALRELSAAREEVPGRRGYPGYMYTDLATIYERAGRVHSKKGSITQLPILTMPDDDITHPIP
DLTGYITEGQLVLSRQLWLKGIYPPFDPLMSLSRLMKDGIGEGKTREDHNNVFMQLISAYAEGIRLRELSVIVGTETLTQ
RDRKYLEFADRFEREFIGQGEYERRKIEETLDKGWELLAILPEEELKMIKVEYIRKYHPKYKGGNP

Specific function: Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit [H]

COG id: COG1156

COG function: function code C; Archaeal/vacuolar-type H+-ATPase subunit B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family [H]

Homologues:

Organism=Homo sapiens, GI19913428, Length=453, Percent_Identity=56.0706401766004, Blast_Score=514, Evalue=1e-146,
Organism=Homo sapiens, GI19913426, Length=457, Percent_Identity=54.0481400437637, Blast_Score=500, Evalue=1e-141,
Organism=Homo sapiens, GI32189394, Length=353, Percent_Identity=27.1954674220963, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI50345984, Length=288, Percent_Identity=28.8194444444444, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4757810, Length=288, Percent_Identity=28.8194444444444, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI19913424, Length=318, Percent_Identity=25.4716981132075, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788251, Length=299, Percent_Identity=31.7725752508361, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790170, Length=404, Percent_Identity=26.7326732673267, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790172, Length=296, Percent_Identity=26.3513513513513, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17570191, Length=459, Percent_Identity=55.3376906318083, Blast_Score=511, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17510931, Length=453, Percent_Identity=55.1876379690949, Blast_Score=511, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71988063, Length=430, Percent_Identity=26.2790697674419, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71988080, Length=290, Percent_Identity=28.6206896551724, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25144756, Length=348, Percent_Identity=26.4367816091954, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71988074, Length=423, Percent_Identity=25.0591016548463, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17565854, Length=327, Percent_Identity=25.0764525993884, Blast_Score=92, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6319603, Length=451, Percent_Identity=56.7627494456763, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6322581, Length=347, Percent_Identity=28.2420749279539, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319370, Length=338, Percent_Identity=30.1775147928994, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320016, Length=227, Percent_Identity=25.9911894273128, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281361666, Length=454, Percent_Identity=55.2863436123348, Blast_Score=503, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24646341, Length=454, Percent_Identity=55.2863436123348, Blast_Score=503, Evalue=1e-142,
Organism=Drosophila melanogaster, GI17136796, Length=454, Percent_Identity=55.2863436123348, Blast_Score=503, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24638766, Length=348, Percent_Identity=27.0114942528736, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24658560, Length=288, Percent_Identity=28.4722222222222, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28574560, Length=351, Percent_Identity=28.2051282051282, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129479, Length=320, Percent_Identity=25, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583992, Length=318, Percent_Identity=24.8427672955975, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583988, Length=320, Percent_Identity=25, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24583986, Length=320, Percent_Identity=25, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24583984, Length=320, Percent_Identity=25, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR000793
- InterPro:   IPR018538
- InterPro:   IPR022879 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF00306 ATP-synt_ab_C; PF09378 HAS-barrel [H]

EC number: 3.6.3.14

Molecular weight: Translated: 52133; Mature: 52002

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKLTTRISSKLLKAQGGLLFAETIPGVTYGEIVEVTLPTSEVKLGQVIDVSKGVTVIQ
CCEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCEEECCCCCEEEE
VFGGVADVDLSRSRVKYRGETLKIPVSIDMLGRIFDGLGRPIDGGPRIIPEDYLDINGAP
ECCCCCCCCCCHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
LNPASRLPPSEFIETGISAIDGLFSIVRGQKLPIFSGSGLPHNRIAAQIVRQARVLGKEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCC
KFAIVFAAIGVPYDDAMFFIDNFKKTGALERTVALINTASSPVIERIATPRVALTIAEHL
CEEEEEEEECCCCCCHHHHHHCCHHCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHH
AWTHDMHVLVILTDMTFYCEALRELSAAREEVPGRRGYPGYMYTDLATIYERAGRVHSKK
HHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
GSITQLPILTMPDDDITHPIPDLTGYITEGQLVLSRQLWLKGIYPPFDPLMSLSRLMKDG
CCEEEEEEEECCCCCCCCCCCCHHCEECCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHCC
IGEGKTREDHNNVFMQLISAYAEGIRLRELSVIVGTETLTQRDRKYLEFADRFEREFIGQ
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
GEYERRKIEETLDKGWELLAILPEEELKMIKVEYIRKYHPKYKGGNP
CHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVKLTTRISSKLLKAQGGLLFAETIPGVTYGEIVEVTLPTSEVKLGQVIDVSKGVTVIQ
CEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCEEECCCCCEEEE
VFGGVADVDLSRSRVKYRGETLKIPVSIDMLGRIFDGLGRPIDGGPRIIPEDYLDINGAP
ECCCCCCCCCCHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
LNPASRLPPSEFIETGISAIDGLFSIVRGQKLPIFSGSGLPHNRIAAQIVRQARVLGKEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCC
KFAIVFAAIGVPYDDAMFFIDNFKKTGALERTVALINTASSPVIERIATPRVALTIAEHL
CEEEEEEEECCCCCCHHHHHHCCHHCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHH
AWTHDMHVLVILTDMTFYCEALRELSAAREEVPGRRGYPGYMYTDLATIYERAGRVHSKK
HHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
GSITQLPILTMPDDDITHPIPDLTGYITEGQLVLSRQLWLKGIYPPFDPLMSLSRLMKDG
CCEEEEEEEECCCCCCCCCCCCHHCEECCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHCC
IGEGKTREDHNNVFMQLISAYAEGIRLRELSVIVGTETLTQRDRKYLEFADRFEREFIGQ
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
GEYERRKIEETLDKGWELLAILPEEELKMIKVEYIRKYHPKYKGGNP
CHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Borate; Diphosphate; HCO3- [C]

Metal ions: Co2+; Fe2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; H+

Specific reaction: ATP + H2O + H+(in) = ADP + phosphate + H+(out)

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Ca2+; CN-; Efrapeptin; Ethidiumbromide; Guanidines various reactions catalyzed by ATPase, analogs; Oligomycin; Quercetin; Trialkyl tinderivatives; Venturicidin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA