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The map label for this gene is glpK [H]

Identifier: 218884193

GI number: 218884193

Start: 845058

End: 846632

Strand: Reverse

Name: glpK [H]

Synonym: DKAM_0882

Alternate gene names: 218884193

Gene position: 846632-845058 (Counterclockwise)

Preceding gene: 218884195

Following gene: 218884192

Centisome position: 62.01

GC content: 48.13

Gene sequence:

>1575_bases
TTGAGTGATGGCTATATTTTATCCATAGATCAAGGCACTACAGGCACTAGGGCAATCCTCTTCGATCATGAAGGTCTACC
TGTTAAAATGGCTTACAGAGAACATAGACAAATATATCCGAGGCCTGGCTGGGTCGAGCATGATCCCCTGGAGATATGGG
TTAACACGAGGTATGTTATCAAGCAGGTTCTCGAGGGTAGCGGTGTTAATCCAGGTGAGATTAAGGCTATAGGGGTCACG
AATCAGCGGGAAACCATACTCCTCTGGGATCCCGAGACAGGTAATCCTGCCTACAATGCTATTGTGTGGCAGGATAGGAG
GACTGCTAGGGAGGTTGAATACTTAAAGAGTAAATACATAGATCTCATACGCTCTAAGACCGGGCTTGTACCCGACAGCT
ACTTCTCTAGCACTAAGATATGGTGGATCCTAGACAACGTGCCTGGTGTAAGGGAAAAGGCGGTAAGCGGCCGGGTTATA
GCCGGCACCATTGATACGTGGATTATATGGAATCTGACAAGGAATTCAAGAGATGTGTTGACACCAGAGAAGAAGGGGGC
TCATGTAACCGATTACTCAAACGCTTCTAGAACCATGCTCTTCGACATACAGAGGATCGATTGGGACAGGGAGCTACTTG
AGGTACAGGGTAGGATACCCTTGGAAATACTCCCGCTTCCACGCCCCTCAAGCGATAAAAGTCTTTACGGCTATGTGGGT
CCAGAGATCGCTGATGAATTATTCCATGGATACAGGGTACCTATAGCTGGAGCTGTGGGTGATCAGCAGGCTGCATTATT
TGGTCAAGCTGGATTCGAGCAAGGCGATATAAAGTGTACTTATGGCACCGGGAACTTCATACTGGTTAACACTGGGGCTA
CACCAGTTTTCTCTAAGCATGGATTGATCACAACGATCTACTATAGTATTGAACCTGGTAAAGCAGTATACGCCCTAGAG
GGAAGCATATTTGTAACCGGGGCGGCGATACAATGGCTGCGCGATGGGTTAAAGATAATAGAGGTCTCAGCCGAGGTCAA
CCCGCTTGCAGAATCGGTGAGCGATACAGGTGGAGTATACTTTGTCCCAGCGTTCACCGGCCTAGGTGCACCCTACTGGG
ATCAATACGCTAGAGGCCTCATAATTGGTATAACAAGAGGCACCGAGAGGAAGCACCTGGCAAGAGCGGTACTGGAATCC
ATAGCCTACTTAACCAGGGATGTTGTTGAAATAGTGGAGCTTGAGACAGGTTCGGAGATAAAGGTCTTAAAGGCTGATGG
TGGTGCATCTAGAAGCGACTTCCTGATGCAGTTCCAAGCGGATATATTGAATACAAAAGTAGTTAGGCCAATGGTGTATG
AGACTACATCGCTTGGTGCAGCATATCTAGCAGGACTAGCTGTCGACTACTGGAGAAGCATTGATGAACTACGTAGATAC
TGGAGGGCCGAGAAAGAGTTCATCCCGAGAATCAGTGAGGAGATAAGGGAAAGGCTCTACAAGGGGTGGAAGGAGGCTGT
TAAGAGGGCGTTAGGATGGGCCCGTGAAGTACCATGGGCCTATGGATATGAGTAA

Upstream 100 bases:

>100_bases
GTATTTCATCCTTAGCAAATATATTAGCTTTTACTACACGGGTTCTTTGATACTGTTTAAATTCCTCAGGTGTTGATCTA
GATATCAGGAGGTACCGGTC

Downstream 100 bases:

>100_bases
AGACGTTTAATCCCTTGCCTGATTATAGACTGATCATAGATTACGGGAGGATTCGTGAATGAGGATAGTTATCATTGGAG
CCGGTGTAGTAGGGTTATCC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSDGYILSIDQGTTGTRAILFDHEGLPVKMAYREHRQIYPRPGWVEHDPLEIWVNTRYVIKQVLEGSGVNPGEIKAIGVT
NQRETILLWDPETGNPAYNAIVWQDRRTAREVEYLKSKYIDLIRSKTGLVPDSYFSSTKIWWILDNVPGVREKAVSGRVI
AGTIDTWIIWNLTRNSRDVLTPEKKGAHVTDYSNASRTMLFDIQRIDWDRELLEVQGRIPLEILPLPRPSSDKSLYGYVG
PEIADELFHGYRVPIAGAVGDQQAALFGQAGFEQGDIKCTYGTGNFILVNTGATPVFSKHGLITTIYYSIEPGKAVYALE
GSIFVTGAAIQWLRDGLKIIEVSAEVNPLAESVSDTGGVYFVPAFTGLGAPYWDQYARGLIIGITRGTERKHLARAVLES
IAYLTRDVVEIVELETGSEIKVLKADGGASRSDFLMQFQADILNTKVVRPMVYETTSLGAAYLAGLAVDYWRSIDELRRY
WRAEKEFIPRISEEIRERLYKGWKEAVKRALGWAREVPWAYGYE

Sequences:

>Translated_524_residues
MSDGYILSIDQGTTGTRAILFDHEGLPVKMAYREHRQIYPRPGWVEHDPLEIWVNTRYVIKQVLEGSGVNPGEIKAIGVT
NQRETILLWDPETGNPAYNAIVWQDRRTAREVEYLKSKYIDLIRSKTGLVPDSYFSSTKIWWILDNVPGVREKAVSGRVI
AGTIDTWIIWNLTRNSRDVLTPEKKGAHVTDYSNASRTMLFDIQRIDWDRELLEVQGRIPLEILPLPRPSSDKSLYGYVG
PEIADELFHGYRVPIAGAVGDQQAALFGQAGFEQGDIKCTYGTGNFILVNTGATPVFSKHGLITTIYYSIEPGKAVYALE
GSIFVTGAAIQWLRDGLKIIEVSAEVNPLAESVSDTGGVYFVPAFTGLGAPYWDQYARGLIIGITRGTERKHLARAVLES
IAYLTRDVVEIVELETGSEIKVLKADGGASRSDFLMQFQADILNTKVVRPMVYETTSLGAAYLAGLAVDYWRSIDELRRY
WRAEKEFIPRISEEIRERLYKGWKEAVKRALGWAREVPWAYGYE
>Mature_523_residues
SDGYILSIDQGTTGTRAILFDHEGLPVKMAYREHRQIYPRPGWVEHDPLEIWVNTRYVIKQVLEGSGVNPGEIKAIGVTN
QRETILLWDPETGNPAYNAIVWQDRRTAREVEYLKSKYIDLIRSKTGLVPDSYFSSTKIWWILDNVPGVREKAVSGRVIA
GTIDTWIIWNLTRNSRDVLTPEKKGAHVTDYSNASRTMLFDIQRIDWDRELLEVQGRIPLEILPLPRPSSDKSLYGYVGP
EIADELFHGYRVPIAGAVGDQQAALFGQAGFEQGDIKCTYGTGNFILVNTGATPVFSKHGLITTIYYSIEPGKAVYALEG
SIFVTGAAIQWLRDGLKIIEVSAEVNPLAESVSDTGGVYFVPAFTGLGAPYWDQYARGLIIGITRGTERKHLARAVLESI
AYLTRDVVEIVELETGSEIKVLKADGGASRSDFLMQFQADILNTKVVRPMVYETTSLGAAYLAGLAVDYWRSIDELRRYW
RAEKEFIPRISEEIRERLYKGWKEAVKRALGWAREVPWAYGYE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI42794763, Length=519, Percent_Identity=44.1233140655106, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI4504007, Length=517, Percent_Identity=43.9071566731141, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI189409120, Length=517, Percent_Identity=43.9071566731141, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI41393575, Length=516, Percent_Identity=42.2480620155039, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI88196792, Length=528, Percent_Identity=32.9545454545455, Blast_Score=299, Evalue=4e-81,
Organism=Escherichia coli, GI1790361, Length=510, Percent_Identity=52.156862745098, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1789987, Length=509, Percent_Identity=24.3614931237721, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1787789, Length=527, Percent_Identity=24.0986717267552, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=523, Percent_Identity=40.5353728489484, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71986140, Length=537, Percent_Identity=26.6294227188082, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=31.3333333333333, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321755, Length=562, Percent_Identity=34.3416370106762, Blast_Score=300, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24655555, Length=521, Percent_Identity=40.4990403071017, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655560, Length=521, Percent_Identity=40.4990403071017, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655570, Length=521, Percent_Identity=40.4990403071017, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655574, Length=521, Percent_Identity=40.4990403071017, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655565, Length=521, Percent_Identity=40.4990403071017, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17864214, Length=529, Percent_Identity=38.7523629489603, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24654573, Length=449, Percent_Identity=40.7572383073497, Blast_Score=324, Evalue=9e-89,
Organism=Drosophila melanogaster, GI19922058, Length=548, Percent_Identity=33.7591240875912, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24652871, Length=513, Percent_Identity=33.9181286549708, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24656367, Length=524, Percent_Identity=30.1526717557252, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI21357867, Length=536, Percent_Identity=28.3582089552239, Blast_Score=232, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24656372, Length=357, Percent_Identity=32.2128851540616, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI45552909, Length=301, Percent_Identity=29.9003322259136, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 58984; Mature: 58853

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGYILSIDQGTTGTRAILFDHEGLPVKMAYREHRQIYPRPGWVEHDPLEIWVNTRYVI
CCCCEEEEECCCCCCCEEEEEECCCCCEEEEEHHHHHCCCCCCCCCCCCEEEEECCHHHH
KQVLEGSGVNPGEIKAIGVTNQRETILLWDPETGNPAYNAIVWQDRRTAREVEYLKSKYI
HHHHCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
DLIRSKTGLVPDSYFSSTKIWWILDNVPGVREKAVSGRVIAGTIDTWIIWNLTRNSRDVL
HHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCEEEEEEECEEEEEEEECCCCCCC
TPEKKGAHVTDYSNASRTMLFDIQRIDWDRELLEVQGRIPLEILPLPRPSSDKSLYGYVG
CCCCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCCCCCEEECCC
PEIADELFHGYRVPIAGAVGDQQAALFGQAGFEQGDIKCTYGTGNFILVNTGATPVFSKH
HHHHHHHHCCCCCEEECCCCCCHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCEECC
GLITTIYYSIEPGKAVYALEGSIFVTGAAIQWLRDGLKIIEVSAEVNPLAESVSDTGGVY
CEEEEEEEEECCCCEEEEECCCEEEEHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCEE
FVPAFTGLGAPYWDQYARGLIIGITRGTERKHLARAVLESIAYLTRDVVEIVELETGSEI
EEECCCCCCCCHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
KVLKADGGASRSDFLMQFQADILNTKVVRPMVYETTSLGAAYLAGLAVDYWRSIDELRRY
EEEECCCCCCHHHHHHHHHHHHHHHHHHCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
WRAEKEFIPRISEEIRERLYKGWKEAVKRALGWAREVPWAYGYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDGYILSIDQGTTGTRAILFDHEGLPVKMAYREHRQIYPRPGWVEHDPLEIWVNTRYVI
CCCEEEEECCCCCCCEEEEEECCCCCEEEEEHHHHHCCCCCCCCCCCCEEEEECCHHHH
KQVLEGSGVNPGEIKAIGVTNQRETILLWDPETGNPAYNAIVWQDRRTAREVEYLKSKYI
HHHHCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
DLIRSKTGLVPDSYFSSTKIWWILDNVPGVREKAVSGRVIAGTIDTWIIWNLTRNSRDVL
HHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCEEEEEEECEEEEEEEECCCCCCC
TPEKKGAHVTDYSNASRTMLFDIQRIDWDRELLEVQGRIPLEILPLPRPSSDKSLYGYVG
CCCCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCCCCCEEECCC
PEIADELFHGYRVPIAGAVGDQQAALFGQAGFEQGDIKCTYGTGNFILVNTGATPVFSKH
HHHHHHHHCCCCCEEECCCCCCHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCEECC
GLITTIYYSIEPGKAVYALEGSIFVTGAAIQWLRDGLKIIEVSAEVNPLAESVSDTGGVY
CEEEEEEEEECCCCEEEEECCCEEEEHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCEE
FVPAFTGLGAPYWDQYARGLIIGITRGTERKHLARAVLESIAYLTRDVVEIVELETGSEI
EEECCCCCCCCHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
KVLKADGGASRSDFLMQFQADILNTKVVRPMVYETTSLGAAYLAGLAVDYWRSIDELRRY
EEEECCCCCCHHHHHHHHHHHHHHHHHHCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
WRAEKEFIPRISEEIRERLYKGWKEAVKRALGWAREVPWAYGYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]