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The map label for this gene is thiD [H]

Identifier: 218884025

GI number: 218884025

Start: 684814

End: 686178

Strand: Direct

Name: thiD [H]

Synonym: DKAM_0714

Alternate gene names: 218884025

Gene position: 684814-686178 (Clockwise)

Preceding gene: 218884023

Following gene: 218884027

Centisome position: 50.16

GC content: 51.94

Gene sequence:

>1365_bases
ATGGAGGAGACACACTTGAAACCCATTCCGGTCGCGTTAACCATAGCTGGAAGCGATTCAGGCGGCGGAGCAGGGATAGA
GGCGGATCTCAAGACATTCACAGCTCTAGGCGTTCACGGTGCTGTAGCAGTCACCAGTGTAACAGCGCAGAACACAGTGG
CTGTCACGGCTATCCATGATATTCCGCCTGAGATCGTTTATAAGCAAATAGAAGCCGTGGCCGAGGACATGGGGGTTGAT
GCTGCCAAGACAGGTATGCTGAGTAATAGCGGGATCATAGAGGTCGTGGCTAGAGCGGTGAATAGATATGGGTTCCCACT
CGTAGTAGATCCAGTCATGATAGCTAAAACCGGGGCCAGGCTCCTCAGAGAGGATGCTGTTAGAGCACTCGTCGAGAAGC
TACTTCCCCTGGCTAAAGTAGCCACCCCGAATAAGCCTGAGGCAGAGGTGTTGGCGGGCATGGAGATAAGGAGCCTTGAG
GATGCTAAGAGGGCTGCTAGGGTAATAGCTGTTGAAACAGGTGTCGAGGCGGTTGTGGTGAAAGGCGGGCATATGGAGGG
TGATGAGTCAATAGATGTCCTCTACTGGCGCGGCGGGTATAGGGAGTACAGGGCTTCAAGGGTGAGCCAGCCCTGCAACC
ATGGAGGTGGATGCAGCTTCTCAGCGGCTATAGCCGCTGGGCTGGCCAAGGGACTCGAGATCCCTGAGGCAGTGAAGTTA
GCCAAGGAATTCATATCCACGGCTATAAGCTACGGGTATAGTGTGGGGAAGGGATATTGTCCAGTGAACCCTGCAGCATG
GCTCGCTATACCTGCTGAAAAATACTGGGCTATAATGGATGTAGAGGAAGCCGTTGAACTAGTCCTAGAGAACAGGGATG
TATTCAAGCATCTGGTCCCCGAGGTAGGAATGAATATAGCTAGGATCATAAACCCCCTGTACGCCAGGGGAATAAAAGAC
GTTGTAGCAGTGGCAGGTAGGATAGTACGGTACATGGGCACGGTGAAGCAGGTGGGCCCCGTGAAGCTGGGGGCGTCGAG
CCACCTGGCAAGAGCTCTCCTAGAGGCAGTGAAGCACAACCCAGAGTTAAGGGCAGTAGTTAACGTAGCCTACAGTAGGG
AGTTCATTGAGAAAGCAGCTAGGAAAGGATACAGGGTAGTCTACATAGATAGAACACGTGAACCGGTAGAAGTAAAGCAG
AAAGAGGGTGCGTCAATACCATGGATAATAAGGGAGGCATTCTCGATAGACAGCAAGGCAGACATAGTCTACGATACAGG
AGATATTGGTAAAGAAGCAATGATCAGGGTATTCGCGGGAACAGCAGTACACGCAGTAATGAAAATACTAGACGTAGCGA
GCTGA

Upstream 100 bases:

>100_bases
CTCGGGTTATATGGGATTCAAGGCTCATGGTTTCACCACTTGTTTCTATCCTAGGTTAAACTTATAAATTTGTTATTGTA
CCATGTAATACTTGGTTATC

Downstream 100 bases:

>100_bases
GTGTTCATCCGAGCTATGTCCTCATGTTACATAAGCACGTAGCATCAGCCTGTGCGGCATAGAAAACATGTTTTAATACA
TATAGCGCGCCACCACTTCT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MEETHLKPIPVALTIAGSDSGGGAGIEADLKTFTALGVHGAVAVTSVTAQNTVAVTAIHDIPPEIVYKQIEAVAEDMGVD
AAKTGMLSNSGIIEVVARAVNRYGFPLVVDPVMIAKTGARLLREDAVRALVEKLLPLAKVATPNKPEAEVLAGMEIRSLE
DAKRAARVIAVETGVEAVVVKGGHMEGDESIDVLYWRGGYREYRASRVSQPCNHGGGCSFSAAIAAGLAKGLEIPEAVKL
AKEFISTAISYGYSVGKGYCPVNPAAWLAIPAEKYWAIMDVEEAVELVLENRDVFKHLVPEVGMNIARIINPLYARGIKD
VVAVAGRIVRYMGTVKQVGPVKLGASSHLARALLEAVKHNPELRAVVNVAYSREFIEKAARKGYRVVYIDRTREPVEVKQ
KEGASIPWIIREAFSIDSKADIVYDTGDIGKEAMIRVFAGTAVHAVMKILDVAS

Sequences:

>Translated_454_residues
MEETHLKPIPVALTIAGSDSGGGAGIEADLKTFTALGVHGAVAVTSVTAQNTVAVTAIHDIPPEIVYKQIEAVAEDMGVD
AAKTGMLSNSGIIEVVARAVNRYGFPLVVDPVMIAKTGARLLREDAVRALVEKLLPLAKVATPNKPEAEVLAGMEIRSLE
DAKRAARVIAVETGVEAVVVKGGHMEGDESIDVLYWRGGYREYRASRVSQPCNHGGGCSFSAAIAAGLAKGLEIPEAVKL
AKEFISTAISYGYSVGKGYCPVNPAAWLAIPAEKYWAIMDVEEAVELVLENRDVFKHLVPEVGMNIARIINPLYARGIKD
VVAVAGRIVRYMGTVKQVGPVKLGASSHLARALLEAVKHNPELRAVVNVAYSREFIEKAARKGYRVVYIDRTREPVEVKQ
KEGASIPWIIREAFSIDSKADIVYDTGDIGKEAMIRVFAGTAVHAVMKILDVAS
>Mature_454_residues
MEETHLKPIPVALTIAGSDSGGGAGIEADLKTFTALGVHGAVAVTSVTAQNTVAVTAIHDIPPEIVYKQIEAVAEDMGVD
AAKTGMLSNSGIIEVVARAVNRYGFPLVVDPVMIAKTGARLLREDAVRALVEKLLPLAKVATPNKPEAEVLAGMEIRSLE
DAKRAARVIAVETGVEAVVVKGGHMEGDESIDVLYWRGGYREYRASRVSQPCNHGGGCSFSAAIAAGLAKGLEIPEAVKL
AKEFISTAISYGYSVGKGYCPVNPAAWLAIPAEKYWAIMDVEEAVELVLENRDVFKHLVPEVGMNIARIINPLYARGIKD
VVAVAGRIVRYMGTVKQVGPVKLGASSHLARALLEAVKHNPELRAVVNVAYSREFIEKAARKGYRVVYIDRTREPVEVKQ
KEGASIPWIIREAFSIDSKADIVYDTGDIGKEAMIRVFAGTAVHAVMKILDVAS

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=256, Percent_Identity=39.84375, Blast_Score=177, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6325378, Length=281, Percent_Identity=34.5195729537367, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324997, Length=282, Percent_Identity=36.5248226950355, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324517, Length=282, Percent_Identity=35.1063829787234, Blast_Score=146, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 48614; Mature: 48614

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETHLKPIPVALTIAGSDSGGGAGIEADLKTFTALGVHGAVAVTSVTAQNTVAVTAIHD
CCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHEEEEECCCEEEEEEECC
IPPEIVYKQIEAVAEDMGVDAAKTGMLSNSGIIEVVARAVNRYGFPLVVDPVMIAKTGAR
CCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEECHHHHHHHHHH
LLREDAVRALVEKLLPLAKVATPNKPEAEVLAGMEIRSLEDAKRAARVIAVETGVEAVVV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHEEEEEECCCEEEEE
KGGHMEGDESIDVLYWRGGYREYRASRVSQPCNHGGGCSFSAAIAAGLAKGLEIPEAVKL
ECCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
AKEFISTAISYGYSVGKGYCPVNPAAWLAIPAEKYWAIMDVEEAVELVLENRDVFKHLVP
HHHHHHHHHHHCHHCCCCCCCCCCCCEEEECHHHHEEEECHHHHHHHHHCCHHHHHHHHH
EVGMNIARIINPLYARGIKDVVAVAGRIVRYMGTVKQVGPVKLGASSHLARALLEAVKHN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCC
PELRAVVNVAYSREFIEKAARKGYRVVYIDRTREPVEVKQKEGASIPWIIREAFSIDSKA
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHHHCCCCCC
DIVYDTGDIGKEAMIRVFAGTAVHAVMKILDVAS
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEETHLKPIPVALTIAGSDSGGGAGIEADLKTFTALGVHGAVAVTSVTAQNTVAVTAIHD
CCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHEEEEECCCEEEEEEECC
IPPEIVYKQIEAVAEDMGVDAAKTGMLSNSGIIEVVARAVNRYGFPLVVDPVMIAKTGAR
CCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEECHHHHHHHHHH
LLREDAVRALVEKLLPLAKVATPNKPEAEVLAGMEIRSLEDAKRAARVIAVETGVEAVVV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHEEEEEECCCEEEEE
KGGHMEGDESIDVLYWRGGYREYRASRVSQPCNHGGGCSFSAAIAAGLAKGLEIPEAVKL
ECCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
AKEFISTAISYGYSVGKGYCPVNPAAWLAIPAEKYWAIMDVEEAVELVLENRDVFKHLVP
HHHHHHHHHHHCHHCCCCCCCCCCCCEEEECHHHHEEEECHHHHHHHHHCCHHHHHHHHH
EVGMNIARIINPLYARGIKDVVAVAGRIVRYMGTVKQVGPVKLGASSHLARALLEAVKHN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCC
PELRAVVNVAYSREFIEKAARKGYRVVYIDRTREPVEVKQKEGASIPWIIREAFSIDSKA
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHHHCCCCCC
DIVYDTGDIGKEAMIRVFAGTAVHAVMKILDVAS
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]