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The map label for this gene is frvX [C]

Identifier: 218883900

GI number: 218883900

Start: 550849

End: 551958

Strand: Reverse

Name: frvX [C]

Synonym: DKAM_0589

Alternate gene names: 218883900

Gene position: 551958-550849 (Counterclockwise)

Preceding gene: 218883901

Following gene: 218883899

Centisome position: 40.43

GC content: 46.22

Gene sequence:

>1110_bases
GTGAACACCTTGGAATGGAGGGGCCAGTACATAGAGTTAATTAAGAAGCTAACCAGCCTTCATGCTCCTAGTGGAAGAGA
AGACCCGGTGAAAGACCTCGTTGCCGAGTTGATGAAGTCTCATGTAGATAAACTATGGATAGACGTATGGGGGAACGTAG
TTGGATATAGGAAGGGCTCTAAGGGCTCTGGTAAAATCATGATCGCAGCCCACATGGATGAAATAGGGTTATTCATCTCT
CACATCGAGGATGACGGGTTTCTAAGGGTTATACCCATAGGTGGAGTACTCGAGAGGACTCTTCTCTACCAAAGAGTAGT
TGTTAGGACGAGGGATGGGAGACTATACAGGGGTGTGATAGGGTTAAAGCCTCCTCATGTAATTAAGCCGGAGGAAGCCC
AGAAAGTACCGGAGCTACGGGAACTCTTCATAGATGTTGGTGCGTCATCAAAGGAGGAAGTGGAGAAGATGGGTATAAGA
GTCGGCGATATAGCTGTATTTGATAGAGAGGTAGCTGAGTTAGGCTGGAACAGGATAACCGGTAAGGCGTTCGATGACAG
GGTTGGCGTCGTGGTTATGTTAAAGGCTCTTGAAATGCTTGAGAAACACGATGTAGACGTTTATTTCGTTGCAACAGTCC
AGGAGGAAGTAGGGTTGAAGGGGGCTAAGACGTCGGCTTACGGTATCTCACCGGATGTGGCATTAGCGATAGATGTAACC
ATAGCAAGCGATGTACCAGGAGTAGCTAAAAGCGAGTGGTTCACTCGGCTAGGATATGGTCCAGCTATAAAGATAGTTGA
TGGGAGAAATGCAGGTGGGTTGATAGCGCATCCAAAGGTGGTTGAGTTCCTGGTGAGCATTGCCGAGAAAAAGAGGATAC
CTTATCAATTAGATGTGATCTCAGGTGGTACAACCGATGCCTCAACAATAGCTTTAAACAAGGAGGGTGTTGCTGCTGGA
ACGATCTCTATTCCCTCAAGGTATATACATAGCCCTGTTGAAGTAGTGGATTTAAGAGACCTCTACAATGCATCATTACT
AGCTAAGGCCTTCATAGAGGAGGCAACGCCTGAGTGGATTCAAAGCATAAAGGGAGTAGTAATAAAATAA

Upstream 100 bases:

>100_bases
AAACTATAATAAGCTACTTGATACAGAGAAAGTATTGAGCATTCTCTTCCACTGATCAAGGTATATTTTTATTCTACATG
TAAACTATATATCCACACTG

Downstream 100 bases:

>100_bases
GCTATGAGGAGCCTGTTGTGGGCTCTAAATTATTTTTAAATTCTTAATATTTTTGAACACGTTTTATTCTCTCGACGCCT
CTACTCGCTTTCCTTGATGA

Product: Aminopeptidase from family M42

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNTLEWRGQYIELIKKLTSLHAPSGREDPVKDLVAELMKSHVDKLWIDVWGNVVGYRKGSKGSGKIMIAAHMDEIGLFIS
HIEDDGFLRVIPIGGVLERTLLYQRVVVRTRDGRLYRGVIGLKPPHVIKPEEAQKVPELRELFIDVGASSKEEVEKMGIR
VGDIAVFDREVAELGWNRITGKAFDDRVGVVVMLKALEMLEKHDVDVYFVATVQEEVGLKGAKTSAYGISPDVALAIDVT
IASDVPGVAKSEWFTRLGYGPAIKIVDGRNAGGLIAHPKVVEFLVSIAEKKRIPYQLDVISGGTTDASTIALNKEGVAAG
TISIPSRYIHSPVEVVDLRDLYNASLLAKAFIEEATPEWIQSIKGVVIK

Sequences:

>Translated_369_residues
MNTLEWRGQYIELIKKLTSLHAPSGREDPVKDLVAELMKSHVDKLWIDVWGNVVGYRKGSKGSGKIMIAAHMDEIGLFIS
HIEDDGFLRVIPIGGVLERTLLYQRVVVRTRDGRLYRGVIGLKPPHVIKPEEAQKVPELRELFIDVGASSKEEVEKMGIR
VGDIAVFDREVAELGWNRITGKAFDDRVGVVVMLKALEMLEKHDVDVYFVATVQEEVGLKGAKTSAYGISPDVALAIDVT
IASDVPGVAKSEWFTRLGYGPAIKIVDGRNAGGLIAHPKVVEFLVSIAEKKRIPYQLDVISGGTTDASTIALNKEGVAAG
TISIPSRYIHSPVEVVDLRDLYNASLLAKAFIEEATPEWIQSIKGVVIK
>Mature_369_residues
MNTLEWRGQYIELIKKLTSLHAPSGREDPVKDLVAELMKSHVDKLWIDVWGNVVGYRKGSKGSGKIMIAAHMDEIGLFIS
HIEDDGFLRVIPIGGVLERTLLYQRVVVRTRDGRLYRGVIGLKPPHVIKPEEAQKVPELRELFIDVGASSKEEVEKMGIR
VGDIAVFDREVAELGWNRITGKAFDDRVGVVVMLKALEMLEKHDVDVYFVATVQEEVGLKGAKTSAYGISPDVALAIDVT
IASDVPGVAKSEWFTRLGYGPAIKIVDGRNAGGLIAHPKVVEFLVSIAEKKRIPYQLDVISGGTTDASTIALNKEGVAAG
TISIPSRYIHSPVEVVDLRDLYNASLLAKAFIEEATPEWIQSIKGVVIK

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=353, Percent_Identity=33.9943342776204, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI87082417, Length=345, Percent_Identity=33.6231884057971, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1788727, Length=357, Percent_Identity=31.0924369747899, Blast_Score=152, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 40528; Mature: 40528

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLEWRGQYIELIKKLTSLHAPSGREDPVKDLVAELMKSHVDKLWIDVWGNVVGYRKGS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCC
KGSGKIMIAAHMDEIGLFISHIEDDGFLRVIPIGGVLERTLLYQRVVVRTRDGRLYRGVI
CCCCEEEEEEECHHHHHHHEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEE
GLKPPHVIKPEEAQKVPELRELFIDVGASSKEEVEKMGIRVGDIAVFDREVAELGWNRIT
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEHHHHHHHHCCCCCCC
GKAFDDRVGVVVMLKALEMLEKHDVDVYFVATVQEEVGLKGAKTSAYGISPDVALAIDVT
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEEEE
IASDVPGVAKSEWFTRLGYGPAIKIVDGRNAGGLIAHPKVVEFLVSIAEKKRIPYQLDVI
EECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
SGGTTDASTIALNKEGVAAGTISIPSRYIHSPVEVVDLRDLYNASLLAKAFIEEATPEWI
CCCCCCCEEEEECCCCCEEEEEECCHHHHCCCHHEEEHHHHHHHHHHHHHHHHHCCHHHH
QSIKGVVIK
HHHCCEECC
>Mature Secondary Structure
MNTLEWRGQYIELIKKLTSLHAPSGREDPVKDLVAELMKSHVDKLWIDVWGNVVGYRKGS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCC
KGSGKIMIAAHMDEIGLFISHIEDDGFLRVIPIGGVLERTLLYQRVVVRTRDGRLYRGVI
CCCCEEEEEEECHHHHHHHEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEE
GLKPPHVIKPEEAQKVPELRELFIDVGASSKEEVEKMGIRVGDIAVFDREVAELGWNRIT
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEHHHHHHHHCCCCCCC
GKAFDDRVGVVVMLKALEMLEKHDVDVYFVATVQEEVGLKGAKTSAYGISPDVALAIDVT
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEEEE
IASDVPGVAKSEWFTRLGYGPAIKIVDGRNAGGLIAHPKVVEFLVSIAEKKRIPYQLDVI
EECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
SGGTTDASTIALNKEGVAAGTISIPSRYIHSPVEVVDLRDLYNASLLAKAFIEEATPEWI
CCCCCCCEEEEECCCCCEEEEEECCHHHHCCCHHEEEHHHHHHHHHHHHHHHHHCCHHHH
QSIKGVVIK
HHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]