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The map label for this gene is rsmF [H]

Identifier: 218883897

GI number: 218883897

Start: 547535

End: 548557

Strand: Reverse

Name: rsmF [H]

Synonym: DKAM_0586

Alternate gene names: 218883897

Gene position: 548557-547535 (Counterclockwise)

Preceding gene: 218883898

Following gene: 218883896

Centisome position: 40.18

GC content: 45.75

Gene sequence:

>1023_bases
TTGAGGCTCTATGAGATATATCGCGACATATACTTGGAGCCATCTAAACAGGCAGTCGAGTTGGCGTTAAGATACGGGTA
TCTCCCATACATGGTTCAAAGATACCTTGAGATGCTTGGCCCAGATGAAGCCCTGGAACTTCTTGAAGCATTCGAAAAAC
CTGTTAAACCAGTTGTCAGAGTTAACACGTTACTTATAAGGCCCGGTGAATTAATGGAGAGACTAGAATCCCTGGGATTC
CAGTTAGAGGAAATACCATGGGCTCCCGCTTCATTCTGGGTAGTTAAAACCCCTAGAAGCCCCACTATAGGCTCGACACA
TGAATACTTAAAGGGCTACTACTATATCCACAGAGATGCCTCTAGCCTGATACCAGTGTTACTCCTACTACATAACTATG
AAGGAGACGTCCTCGACGCCTGTGCCGCCCCAGGCGGTAAGGCAACGTTTATGGCTCAAATCCTTAAGGAGCGGGGAAAA
GGTATTGTATACGCTAACGACTATGTCTTATACAGGTTAAAGACCCTGGTGGGGCATCTATTAAGGATGAAACTAGATAA
CGTAGTAGTAGCATGGAGTAATGCGTTAAACCTTCCGGGGAAACTAGGTAGAAGATTTAAGAGGATCCTCCTGGACGCAC
CATGTAGTGGGGAGGGAAGGATATCTGTGGATCCAGGGCGGAAAACACGAACAAGTATCTTAGACCTGGCCATGCTTGCC
AAGAGAGAGATATTGTTGCTGGATAGATTAATAGATCTACTGGATGAAAATGGGGTACTAGCGTACTCAACGTGCAGTAT
TGCACCGGAGGAAAACGAGTACGTTCTTAGTAGGATACTGAATTCAAGGAGCGATATCGAGATAATTGAGCCGGAATTAA
AACTGTTCAATTATAGCCCCTGGCTCACCCATTATAGGTCAATGGAGTTCCCCAGGGACCTTGAGAAATGCATCAGGGTA
TGGCCTCATAGACACGGCTTATTCGGGTTTACCACATGCCTGATCAGGAGGATTAAGTCATGA

Upstream 100 bases:

>100_bases
AGCCCGAGAAAGTATATGTGTTCTTCAATAACGATCACTGGATGCTTGAAAATGCCCGCTCACTAATGGGGATGCTTAGG
GGTTAAGAGGGGTCCTCTGC

Downstream 100 bases:

>100_bases
AGGGGAAGATAAAGTTTAAGACGGGAAGAATTGGGAAAACCCTGTACAACAGGCTCTATAATCTATTTAAGAACACATTC
AACGTTAACCCTGAAGAATA

Product: putative RNA methylase, NOL1/NOP2/sun family

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRLYEIYRDIYLEPSKQAVELALRYGYLPYMVQRYLEMLGPDEALELLEAFEKPVKPVVRVNTLLIRPGELMERLESLGF
QLEEIPWAPASFWVVKTPRSPTIGSTHEYLKGYYYIHRDASSLIPVLLLLHNYEGDVLDACAAPGGKATFMAQILKERGK
GIVYANDYVLYRLKTLVGHLLRMKLDNVVVAWSNALNLPGKLGRRFKRILLDAPCSGEGRISVDPGRKTRTSILDLAMLA
KREILLLDRLIDLLDENGVLAYSTCSIAPEENEYVLSRILNSRSDIEIIEPELKLFNYSPWLTHYRSMEFPRDLEKCIRV
WPHRHGLFGFTTCLIRRIKS

Sequences:

>Translated_340_residues
MRLYEIYRDIYLEPSKQAVELALRYGYLPYMVQRYLEMLGPDEALELLEAFEKPVKPVVRVNTLLIRPGELMERLESLGF
QLEEIPWAPASFWVVKTPRSPTIGSTHEYLKGYYYIHRDASSLIPVLLLLHNYEGDVLDACAAPGGKATFMAQILKERGK
GIVYANDYVLYRLKTLVGHLLRMKLDNVVVAWSNALNLPGKLGRRFKRILLDAPCSGEGRISVDPGRKTRTSILDLAMLA
KREILLLDRLIDLLDENGVLAYSTCSIAPEENEYVLSRILNSRSDIEIIEPELKLFNYSPWLTHYRSMEFPRDLEKCIRV
WPHRHGLFGFTTCLIRRIKS
>Mature_340_residues
MRLYEIYRDIYLEPSKQAVELALRYGYLPYMVQRYLEMLGPDEALELLEAFEKPVKPVVRVNTLLIRPGELMERLESLGF
QLEEIPWAPASFWVVKTPRSPTIGSTHEYLKGYYYIHRDASSLIPVLLLLHNYEGDVLDACAAPGGKATFMAQILKERGK
GIVYANDYVLYRLKTLVGHLLRMKLDNVVVAWSNALNLPGKLGRRFKRILLDAPCSGEGRISVDPGRKTRTSILDLAMLA
KREILLLDRLIDLLDENGVLAYSTCSIAPEENEYVLSRILNSRSDIEIIEPELKLFNYSPWLTHYRSMEFPRDLEKCIRV
WPHRHGLFGFTTCLIRRIKS

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=316, Percent_Identity=35.126582278481, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI76150623, Length=316, Percent_Identity=35.126582278481, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI39995082, Length=328, Percent_Identity=27.1341463414634, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI301336155, Length=297, Percent_Identity=27.6094276094276, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI23199998, Length=238, Percent_Identity=29.8319327731092, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI270288818, Length=238, Percent_Identity=29.8319327731092, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI11545785, Length=230, Percent_Identity=31.304347826087, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI8922322, Length=238, Percent_Identity=29.8319327731092, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI270288816, Length=238, Percent_Identity=29.8319327731092, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI32698918, Length=186, Percent_Identity=31.7204301075269, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI87081985, Length=233, Percent_Identity=33.4763948497854, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI2367212, Length=173, Percent_Identity=32.9479768786127, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536757, Length=349, Percent_Identity=33.2378223495702, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25143471, Length=327, Percent_Identity=26.605504587156, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324268, Length=319, Percent_Identity=33.8557993730408, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319447, Length=374, Percent_Identity=25.668449197861, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI22024126, Length=324, Percent_Identity=33.641975308642, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI21355201, Length=274, Percent_Identity=28.1021897810219, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 39160; Mature: 39160

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYEIYRDIYLEPSKQAVELALRYGYLPYMVQRYLEMLGPDEALELLEAFEKPVKPVVR
CHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHH
VNTLLIRPGELMERLESLGFQLEEIPWAPASFWVVKTPRSPTIGSTHEYLKGYYYIHRDA
HHHEEECCHHHHHHHHHHCCEEHHCCCCCCCEEEEECCCCCCCCCHHHHHCCEEEEECCH
SSLIPVLLLLHNYEGDVLDACAAPGGKATFMAQILKERGKGIVYANDYVLYRLKTLVGHL
HHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHH
LRMKLDNVVVAWSNALNLPGKLGRRFKRILLDAPCSGEGRISVDPGRKTRTSILDLAMLA
HHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHH
KREILLLDRLIDLLDENGVLAYSTCSIAPEENEYVLSRILNSRSDIEIIEPELKLFNYSP
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEECCCHHEECCCH
WLTHYRSMEFPRDLEKCIRVWPHRHGLFGFTTCLIRRIKS
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLYEIYRDIYLEPSKQAVELALRYGYLPYMVQRYLEMLGPDEALELLEAFEKPVKPVVR
CHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHH
VNTLLIRPGELMERLESLGFQLEEIPWAPASFWVVKTPRSPTIGSTHEYLKGYYYIHRDA
HHHEEECCHHHHHHHHHHCCEEHHCCCCCCCEEEEECCCCCCCCCHHHHHCCEEEEECCH
SSLIPVLLLLHNYEGDVLDACAAPGGKATFMAQILKERGKGIVYANDYVLYRLKTLVGHL
HHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHH
LRMKLDNVVVAWSNALNLPGKLGRRFKRILLDAPCSGEGRISVDPGRKTRTSILDLAMLA
HHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHH
KREILLLDRLIDLLDENGVLAYSTCSIAPEENEYVLSRILNSRSDIEIIEPELKLFNYSP
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEECCCHHEECCCH
WLTHYRSMEFPRDLEKCIRVWPHRHGLFGFTTCLIRRIKS
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA