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The map label for this gene is metG [H]

Identifier: 218883858

GI number: 218883858

Start: 513275

End: 514771

Strand: Reverse

Name: metG [H]

Synonym: DKAM_0547

Alternate gene names: 218883858

Gene position: 514771-513275 (Counterclockwise)

Preceding gene: 218883859

Following gene: 218883857

Centisome position: 37.71

GC content: 45.02

Gene sequence:

>1497_bases
GTGACTACACCGATATATTACCCTAATGCACCACCGCATATAGGGCACGCGTATACAACAGTATTCGCTGACGTGCTTGC
AAGATTCCACAGGTTGACTGGTGGCAAGGTCTTCTTCCTAACCGGTAATGATGAACATGGATTGAAGATCCAGAGGGTTG
CAGAGAAGGCGGGAAAACACCCCAAGGAATATGTTGATGAAATGGCCAACCTGTACAGGGAGTACTGGAGAATATTGAAT
ATCTCGTACGACTATTTTATAAGGACAACTGATCCCTACCATGAGAAAGCCGTTAAGGAAGCATTTAACTACATATACGG
GAAGGGCTTGATATATAAGGCGAAGTATAGTGGGATGTATTGCGTGGACTGTGAGAAGTATTATTCACCGGGCGAATACA
CGGAGGTTGAGGGCAAGCCCTACTGTCCAATCCATAACAAGCCCTTGGAGTACCTGGAGGAGGAGACGTATTACTTCAAG
TTATCGGAGTTCAAGGATTACTTACTGGATGTACTTGAGAACCAAGATATAGTGTATCCGTCCCAGTATGCTAGAGAAGT
AGCCTCCAAGATAAGGAATGAGGGACTAAGGGATGTCTCGGTGGCTAGGCCTGTTGAAAGAGTATGGTGGGGTATCCCGG
TTCCATTCGATGACAAATACGTTATCTATGTATGGTTCGATGCTTTAATGAACTATCTCAGCGGGATAGGGTATCCTGAT
GACAAGGAGAAGAACACGCTCTACTGGAGCAACGTGCATCATATAATAGGCAAGGACATACTATGGTTCCATACAGCCGT
ATGGTTCTCCATGCTGAAAGCACTAGATATGCCTCCTCCGAGGAGGGTAATAGTTCACTCATATCTGATCAGCAAGGGCC
TAAAGATAAGTAAGAGCATCGGCAATGTGATATCTATAGAGGAACTAGTGGAGAGATATAATGGTAGCGACGGCGTGAGA
TACCTACTCTCAAGGATATTTAACACTGATAAGGACAGCGAGGTCTCCTTCGAGTTACTTGACAGCATCTATAACACGGA
GCTCGCCGACACATTTGGAAACCTGGTTAGGAGAGTTGGGTCGCTAGCATTAAAAAAGATGGGTGGCAAGGTATATAAGA
GGAGTATTGATGAAAATGTAGCCGATGTAATTAGCAGTGGTATCGGGGCATACCTCGATGCAATGAGGAGTTACGAGGTC
TCTAAGGCTCTAGAGCATGTGATGAACATAGCTAGATACGCTAACCAATATATTAATGAAACCAAGCCCTGGGAAAAGTC
TAATCCAAGCAAGGAGCTGTATACGTTACTGGAGCTGATCAAGGTTGCAACAATACTGCTTCACCCGTTCACGCCAATGG
CATCTTCGAAACTAGCCTCCGCATTCGGGTTTGAAATAGAGAACCCAGTGAACATGGAGCCCGGGAAAACTGAGAGATAC
ACCATCACTGATGCACCAATACTCTTTAGAAAAATCCAGGTTAAACAAGAGGGATAG

Upstream 100 bases:

>100_bases
TATGCCGGCAGGCTTGAGGAAATAATTAAAAAGTGAGACAACATTAATCAACTCTCAATGAATAAGGATTCAGTGGCGGT
CTAACATGAGTGTGTTTTAT

Downstream 100 bases:

>100_bases
ATGGTAGGAATGGCCAGGTATCTGGCGGTGGATCTGGGTGCAACTAAAACCAGGATAGCTCTGTGCGGTCAGGACAGGAT
ACTTGATAAAATAGTTTTTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTTPIYYPNAPPHIGHAYTTVFADVLARFHRLTGGKVFFLTGNDEHGLKIQRVAEKAGKHPKEYVDEMANLYREYWRILN
ISYDYFIRTTDPYHEKAVKEAFNYIYGKGLIYKAKYSGMYCVDCEKYYSPGEYTEVEGKPYCPIHNKPLEYLEEETYYFK
LSEFKDYLLDVLENQDIVYPSQYAREVASKIRNEGLRDVSVARPVERVWWGIPVPFDDKYVIYVWFDALMNYLSGIGYPD
DKEKNTLYWSNVHHIIGKDILWFHTAVWFSMLKALDMPPPRRVIVHSYLISKGLKISKSIGNVISIEELVERYNGSDGVR
YLLSRIFNTDKDSEVSFELLDSIYNTELADTFGNLVRRVGSLALKKMGGKVYKRSIDENVADVISSGIGAYLDAMRSYEV
SKALEHVMNIARYANQYINETKPWEKSNPSKELYTLLELIKVATILLHPFTPMASSKLASAFGFEIENPVNMEPGKTERY
TITDAPILFRKIQVKQEG

Sequences:

>Translated_498_residues
MTTPIYYPNAPPHIGHAYTTVFADVLARFHRLTGGKVFFLTGNDEHGLKIQRVAEKAGKHPKEYVDEMANLYREYWRILN
ISYDYFIRTTDPYHEKAVKEAFNYIYGKGLIYKAKYSGMYCVDCEKYYSPGEYTEVEGKPYCPIHNKPLEYLEEETYYFK
LSEFKDYLLDVLENQDIVYPSQYAREVASKIRNEGLRDVSVARPVERVWWGIPVPFDDKYVIYVWFDALMNYLSGIGYPD
DKEKNTLYWSNVHHIIGKDILWFHTAVWFSMLKALDMPPPRRVIVHSYLISKGLKISKSIGNVISIEELVERYNGSDGVR
YLLSRIFNTDKDSEVSFELLDSIYNTELADTFGNLVRRVGSLALKKMGGKVYKRSIDENVADVISSGIGAYLDAMRSYEV
SKALEHVMNIARYANQYINETKPWEKSNPSKELYTLLELIKVATILLHPFTPMASSKLASAFGFEIENPVNMEPGKTERY
TITDAPILFRKIQVKQEG
>Mature_497_residues
TTPIYYPNAPPHIGHAYTTVFADVLARFHRLTGGKVFFLTGNDEHGLKIQRVAEKAGKHPKEYVDEMANLYREYWRILNI
SYDYFIRTTDPYHEKAVKEAFNYIYGKGLIYKAKYSGMYCVDCEKYYSPGEYTEVEGKPYCPIHNKPLEYLEEETYYFKL
SEFKDYLLDVLENQDIVYPSQYAREVASKIRNEGLRDVSVARPVERVWWGIPVPFDDKYVIYVWFDALMNYLSGIGYPDD
KEKNTLYWSNVHHIIGKDILWFHTAVWFSMLKALDMPPPRRVIVHSYLISKGLKISKSIGNVISIEELVERYNGSDGVRY
LLSRIFNTDKDSEVSFELLDSIYNTELADTFGNLVRRVGSLALKKMGGKVYKRSIDENVADVISSGIGAYLDAMRSYEVS
KALEHVMNIARYANQYINETKPWEKSNPSKELYTLLELIKVATILLHPFTPMASSKLASAFGFEIENPVNMEPGKTERYT
ITDAPILFRKIQVKQEG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=498, Percent_Identity=34.9397590361446, Blast_Score=281, Evalue=9e-76,
Organism=Homo sapiens, GI14043022, Length=511, Percent_Identity=26.2230919765166, Blast_Score=145, Evalue=7e-35,
Organism=Escherichia coli, GI1788432, Length=511, Percent_Identity=25.2446183953033, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI212645096, Length=502, Percent_Identity=32.8685258964143, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI71992781, Length=396, Percent_Identity=30.050505050505, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17540794, Length=514, Percent_Identity=23.7354085603113, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321610, Length=523, Percent_Identity=31.3575525812619, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6321704, Length=553, Percent_Identity=24.5931283905967, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358483, Length=484, Percent_Identity=35.1239669421488, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24655311, Length=524, Percent_Identity=24.0458015267176, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57600; Mature: 57469

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPIYYPNAPPHIGHAYTTVFADVLARFHRLTGGKVFFLTGNDEHGLKIQRVAEKAGKH
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCC
PKEYVDEMANLYREYWRILNISYDYFIRTTDPYHEKAVKEAFNYIYGKGLIYKAKYSGMY
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCEE
CVDCEKYYSPGEYTEVEGKPYCPIHNKPLEYLEEETYYFKLSEFKDYLLDVLENQDIVYP
EEEHHHHCCCCCCEECCCCCCCCCCCCCHHHHCCCHHEEEHHHHHHHHHHHHCCCCCCCC
SQYAREVASKIRNEGLRDVSVARPVERVWWGIPVPFDDKYVIYVWFDALMNYLSGIGYPD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCC
DKEKNTLYWSNVHHIIGKDILWFHTAVWFSMLKALDMPPPRRVIVHSYLISKGLKISKSI
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
GNVISIEELVERYNGSDGVRYLLSRIFNTDKDSEVSFELLDSIYNTELADTFGNLVRRVG
CCEECHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SLALKKMGGKVYKRSIDENVADVISSGIGAYLDAMRSYEVSKALEHVMNIARYANQYINE
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TKPWEKSNPSKELYTLLELIKVATILLHPFTPMASSKLASAFGFEIENPVNMEPGKTERY
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEE
TITDAPILFRKIQVKQEG
EECCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TTPIYYPNAPPHIGHAYTTVFADVLARFHRLTGGKVFFLTGNDEHGLKIQRVAEKAGKH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCC
PKEYVDEMANLYREYWRILNISYDYFIRTTDPYHEKAVKEAFNYIYGKGLIYKAKYSGMY
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCEE
CVDCEKYYSPGEYTEVEGKPYCPIHNKPLEYLEEETYYFKLSEFKDYLLDVLENQDIVYP
EEEHHHHCCCCCCEECCCCCCCCCCCCCHHHHCCCHHEEEHHHHHHHHHHHHCCCCCCCC
SQYAREVASKIRNEGLRDVSVARPVERVWWGIPVPFDDKYVIYVWFDALMNYLSGIGYPD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCC
DKEKNTLYWSNVHHIIGKDILWFHTAVWFSMLKALDMPPPRRVIVHSYLISKGLKISKSI
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
GNVISIEELVERYNGSDGVRYLLSRIFNTDKDSEVSFELLDSIYNTELADTFGNLVRRVG
CCEECHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SLALKKMGGKVYKRSIDENVADVISSGIGAYLDAMRSYEVSKALEHVMNIARYANQYINE
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TKPWEKSNPSKELYTLLELIKVATILLHPFTPMASSKLASAFGFEIENPVNMEPGKTERY
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEE
TITDAPILFRKIQVKQEG
EECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]