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The map label for this gene is glkA [H]

Identifier: 218883857

GI number: 218883857

Start: 512276

End: 513265

Strand: Reverse

Name: glkA [H]

Synonym: DKAM_0546

Alternate gene names: 218883857

Gene position: 513265-512276 (Counterclockwise)

Preceding gene: 218883858

Following gene: 218883855

Centisome position: 37.6

GC content: 47.78

Gene sequence:

>990_bases
ATGGCCAGGTATCTGGCGGTGGATCTGGGTGCAACTAAAACCAGGATAGCTCTGTGCGGTCAGGACAGGATACTTGATAA
AATAGTTTTTCCCACACCTAAAACCGGTGATAATACAACGATAGCTGAGTCAATAGTTGCTAAAGCTAGAGAGAAATGGA
GTAGTATACTAGACAGTGTAGAAGCAGTTGGCGTGGCATCAATAGGTCCACTAGATTTAAGGAGGGGAGTCATTGTTAAG
CCGCCTAATCTATTATTCAATGAAGTAAAGCTACTAGAACCCCTGGAAAGAATGCTCGGTAAGAGAGTATACGTTGTAAA
CGATGCTGTAGCCGCTGCTTGGGGAGAGAAGCACTTTGGCTCTGGAAGAAGCTTCGAGAACCTGCTCTACGTGACGCTGA
GTACTGGTGTGGGGGGCGGTGTAATAGTGAATAATCATCTCTTACTAGGCAAGCAGGGAAACGCACATGAAATAGGCCAC
ATCGTGGTTGACTACAACTCGGAACTCAAGTGCGGTTGCGGCGGCTACGGGCACTGGGAGGCCTACGTTGGTGGAAGAAA
CTTACCAAGAGTAGCGAGGTGGCTACTTGAGAGGAACCCAGGGCTAGCCTCAGGTAGTCTCCTCGCCAAGAGGATCTCAA
TAGGGGAGCAGGTGACAAGTATAGATATATTTACACTATACAGGAGCAATGACCCCCTCGCCTTAGAGGTGGTGAAGCAA
TTTATTAAGGCAACGGGAGCTGGACTCGCTTCAGCGATAAACTCATATGATCCAGAGGTAGTCATCATCGGTGGAGGAGT
ATTCATCAATAACATTGATATACTGCTTGAACCCATAAGGAGGGAAGCAGAAAGAAACATTGTGACAACCCCGCCTCTGA
TACAGCCAACAACGCTTGGAGACGATGTGGGATTATACGGGGCATTAGCGCTGGTTATCGAGCCCCCGGAGGAACTCTTG
AAGCTACAGTCATCAATAACTACTCAGTAA

Upstream 100 bases:

>100_bases
AGTGAACATGGAGCCCGGGAAAACTGAGAGATACACCATCACTGATGCACCAATACTCTTTAGAAAAATCCAGGTTAAAC
AAGAGGGATAGATGGTAGGA

Downstream 100 bases:

>100_bases
ACGCTTCCCGCTGGACAACCCTATATAGTTGACGCTCCACTCTAGAGGTGGCTTCTACTAGTAGTTGGACCACGTACCTG
TATTATGGTGATCGCCTCTA

Product: glucokinase (ROK family protein)

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MARYLAVDLGATKTRIALCGQDRILDKIVFPTPKTGDNTTIAESIVAKAREKWSSILDSVEAVGVASIGPLDLRRGVIVK
PPNLLFNEVKLLEPLERMLGKRVYVVNDAVAAAWGEKHFGSGRSFENLLYVTLSTGVGGGVIVNNHLLLGKQGNAHEIGH
IVVDYNSELKCGCGGYGHWEAYVGGRNLPRVARWLLERNPGLASGSLLAKRISIGEQVTSIDIFTLYRSNDPLALEVVKQ
FIKATGAGLASAINSYDPEVVIIGGGVFINNIDILLEPIRREAERNIVTTPPLIQPTTLGDDVGLYGALALVIEPPEELL
KLQSSITTQ

Sequences:

>Translated_329_residues
MARYLAVDLGATKTRIALCGQDRILDKIVFPTPKTGDNTTIAESIVAKAREKWSSILDSVEAVGVASIGPLDLRRGVIVK
PPNLLFNEVKLLEPLERMLGKRVYVVNDAVAAAWGEKHFGSGRSFENLLYVTLSTGVGGGVIVNNHLLLGKQGNAHEIGH
IVVDYNSELKCGCGGYGHWEAYVGGRNLPRVARWLLERNPGLASGSLLAKRISIGEQVTSIDIFTLYRSNDPLALEVVKQ
FIKATGAGLASAINSYDPEVVIIGGGVFINNIDILLEPIRREAERNIVTTPPLIQPTTLGDDVGLYGALALVIEPPEELL
KLQSSITTQ
>Mature_328_residues
ARYLAVDLGATKTRIALCGQDRILDKIVFPTPKTGDNTTIAESIVAKAREKWSSILDSVEAVGVASIGPLDLRRGVIVKP
PNLLFNEVKLLEPLERMLGKRVYVVNDAVAAAWGEKHFGSGRSFENLLYVTLSTGVGGGVIVNNHLLLGKQGNAHEIGHI
VVDYNSELKCGCGGYGHWEAYVGGRNLPRVARWLLERNPGLASGSLLAKRISIGEQVTSIDIFTLYRSNDPLALEVVKQF
IKATGAGLASAINSYDPEVVIIGGGVFINNIDILLEPIRREAERNIVTTPPLIQPTTLGDDVGLYGALALVIEPPEELLK
LQSSITTQ

Specific function: Required for glucose repression of many different genes [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566315, Length=283, Percent_Identity=29.3286219081272, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI298566325, Length=283, Percent_Identity=29.3286219081272, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4885285, Length=283, Percent_Identity=29.3286219081272, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI190014632, Length=283, Percent_Identity=29.3286219081272, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1786891, Length=297, Percent_Identity=26.5993265993266, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081733, Length=327, Percent_Identity=25.0764525993884, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787878, Length=211, Percent_Identity=26.5402843601896, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787363, Length=322, Percent_Identity=23.6024844720497, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082230, Length=269, Percent_Identity=30.4832713754647, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 35482; Mature: 35351

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYLAVDLGATKTRIALCGQDRILDKIVFPTPKTGDNTTIAESIVAKAREKWSSILDSV
CCEEEEEECCCCEEEEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EAVGVASIGPLDLRRGVIVKPPNLLFNEVKLLEPLERMLGKRVYVVNDAVAAAWGEKHFG
HHCCCCCCCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHCCCHHCC
SGRSFENLLYVTLSTGVGGGVIVNNHLLLGKQGNAHEIGHIVVDYNSELKCGCGGYGHWE
CCCCCCEEEEEEEECCCCCCEEEECEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCEE
AYVGGRNLPRVARWLLERNPGLASGSLLAKRISIGEQVTSIDIFTLYRSNDPLALEVVKQ
EEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHH
FIKATGAGLASAINSYDPEVVIIGGGVFINNIDILLEPIRREAERNIVTTPPLIQPTTLG
HHHHHCCHHHHHHHCCCCCEEEEECCEEEECHHHHHHHHHHHHHCCEECCCCCCCCCCCC
DDVGLYGALALVIEPPEELLKLQSSITTQ
CCCCCCEEEEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARYLAVDLGATKTRIALCGQDRILDKIVFPTPKTGDNTTIAESIVAKAREKWSSILDSV
CEEEEEECCCCEEEEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EAVGVASIGPLDLRRGVIVKPPNLLFNEVKLLEPLERMLGKRVYVVNDAVAAAWGEKHFG
HHCCCCCCCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHCCCHHCC
SGRSFENLLYVTLSTGVGGGVIVNNHLLLGKQGNAHEIGHIVVDYNSELKCGCGGYGHWE
CCCCCCEEEEEEEECCCCCCEEEECEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCEE
AYVGGRNLPRVARWLLERNPGLASGSLLAKRISIGEQVTSIDIFTLYRSNDPLALEVVKQ
EEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHH
FIKATGAGLASAINSYDPEVVIIGGGVFINNIDILLEPIRREAERNIVTTPPLIQPTTLG
HHHHHCCHHHHHHHCCCCCEEEEECCEEEECHHHHHHHHHHHHHCCEECCCCCCCCCCCC
DDVGLYGALALVIEPPEELLKLQSSITTQ
CCCCCCEEEEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435260; 12000953 [H]